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Protein

Importin subunit alpha-7

Gene

Kpna6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

GO - Molecular functioni

GO - Biological processi

  • maternal process involved in female pregnancy Source: MGI
  • NLS-bearing protein import into nucleus Source: GO_Central
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • protein import into nucleus Source: MGI

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit alpha-7
Alternative name(s):
Importin alpha-S2
Karyopherin subunit alpha-6
Gene namesi
Name:Kpna6
Synonyms:Kpna5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1100836 Kpna6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207301 – 536Importin subunit alpha-7Add BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei113PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35345
PaxDbiO35345
PeptideAtlasiO35345
PRIDEiO35345

PTM databases

iPTMnetiO35345
PhosphoSitePlusiO35345

Expressioni

Tissue specificityi

Only slightly detected in Ehrlich ascites tumor cells, thymus and skeletal muscle, clearly detected in kidney, spleen, liver, heart, and lung. High expression in testis.

Gene expression databases

BgeeiENSMUSG00000003731
CleanExiMM_KPNA6
ExpressionAtlasiO35345 baseline and differential
GenevisibleiO35345 MM

Interactioni

Subunit structurei

Forms a complex with importin subunit beta-1 (By similarity). Interacts with ZIC3.By similarity1 Publication

Protein-protein interaction databases

BioGridi201010, 3 interactors
DIPiDIP-48616N
IntActiO35345, 2 interactors
STRINGi10090.ENSMUSP00000099650

Structurei

3D structure databases

ProteinModelPortaliO35345
SMRiO35345
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 60IBBPROSITE-ProRule annotationAdd BLAST60
Repeati76 – 115ARM 1; truncatedAdd BLAST40
Repeati116 – 159ARM 2Add BLAST44
Repeati160 – 204ARM 3Add BLAST45
Repeati205 – 243ARM 4Add BLAST39
Repeati244 – 288ARM 5Add BLAST45
Repeati289 – 328ARM 6Add BLAST40
Repeati329 – 370ARM 7Add BLAST42
Repeati371 – 410ARM 8Add BLAST40
Repeati411 – 453ARM 9Add BLAST43
Repeati457 – 502ARM 10; atypicalAdd BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 239NLS binding site (major)By similarityAdd BLAST93
Regioni316 – 404NLS binding site (minor)By similarityAdd BLAST89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi45 – 54Nuclear localization signalBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi28 – 31Poly-Arg4

Domaini

Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins (By similarity).By similarity
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the importin alpha family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166 Eukaryota
COG5064 LUCA
GeneTreeiENSGT00760000119094
HOGENOMiHOG000167616
HOVERGENiHBG001846
InParanoidiO35345
OMAiPYAITIE
OrthoDBiEOG091G095Z
PhylomeDBiO35345
TreeFamiTF354205

Family and domain databases

Gene3Di1.20.5.690, 1 hit
1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR032413 Arm_3
IPR000225 Armadillo
IPR002652 Importin-a_IBB
IPR036975 Importin-a_IBB_sf
IPR024931 Importing_su_alpha
PfamiView protein in Pfam
PF00514 Arm, 8 hits
PF16186 Arm_3, 1 hit
PF01749 IBB, 1 hit
PIRSFiPIRSF005673 Importin_alpha, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 8 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50176 ARM_REPEAT, 2 hits
PS51214 IBB, 1 hit

Sequencei

Sequence statusi: Complete.

O35345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METMASPGKD NYRMKSYKNN ALNPEEMRRR REEEGIQLRK QKREQQLFKR
60 70 80 90 100
RNVELINEEA AMFDSLLMDS YVSSTTGESV ITREMVEMLF SDDSDLQLAT
110 120 130 140 150
TQKFRKLLSK EPSPPIDEVI NTPGVVDRFV EFLKRNENCT LQFEAAWALT
160 170 180 190 200
NIASGTSQQT KIVIEAGAVP IFIELLNSDF EDVQEQAVWA LGNIAGDSSL
210 220 230 240 250
CRDYVLNCSI LNPLLTLLTK STRLTMTRNA VWALSNLCRG KNPPPEFAKV
260 270 280 290 300
SPCLPVLSRL LFSSDSDLLA DACWALSYLS DGPNEKIQAV IDSGVCRRLV
310 320 330 340 350
ELLMHNDYKV ASPALRAVGN IVTGDDIQTQ VILNCSALPC LLHLLSSSKE
360 370 380 390 400
SIRKEACWTI SNITAGNRAQ IQAVIDANIF PVLIEILQKA EFRTRKEAAW
410 420 430 440 450
AITNATSGGT PEQIRYLVSL GCIKPLCDLL TVMDSKIVQV ALNGLENILR
460 470 480 490 500
LGEQESKRSG SGVNPYCGLI EEAYGLDKIE FLQSHENQEI YQKAFDLIEH
510 520 530
YFGVEDDDSS LAPQVDETQQ QFIFQQPEAP MEGFQL
Length:536
Mass (Da):59,964
Last modified:June 12, 2007 - v2
Checksum:i553A6265023052AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86V → E in BAE32900 (PubMed:16141072).Curated1
Sequence conflicti280S → A in BAE32900 (PubMed:16141072).Curated1
Sequence conflicti435S → T in BAB24841 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007053 mRNA Translation: BAB24841.1
AK154882 mRNA Translation: BAE32900.1
BC004833 mRNA Translation: AAH04833.2
AF020773 mRNA Translation: AAC53373.1
CCDSiCCDS18701.1
RefSeqiNP_032494.3, NM_008468.4
UniGeneiMm.202358

Genome annotation databases

EnsembliENSMUST00000102590; ENSMUSP00000099650; ENSMUSG00000003731
GeneIDi16650
KEGGimmu:16650
UCSCiuc008uxz.2 mouse

Similar proteinsi

Entry informationi

Entry nameiIMA7_MOUSE
AccessioniPrimary (citable) accession number: O35345
Secondary accession number(s): Q3U388, Q9CVP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 12, 2007
Last modified: March 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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