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Protein

Serine/arginine-rich splicing factor 5

Gene

Srsf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be required for progression through G1 and entry into S phase of cell growth. May play a regulatory role in pre-mRNA splicing. Autoregulates its own expression. Plays a role in constitutive splicing and can modulate the selection of alternative splice sites (By similarity).By similarity

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: Ensembl
  • RS domain binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 5
Alternative name(s):
Delayed-early protein HRS
Pre-mRNA-splicing factor SRP40
Splicing factor, arginine/serine-rich 5
Gene namesi
Name:Srsf5
Synonyms:Hrs, Sfrs5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:98287. Srsf5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • nuclear speck Source: UniProtKB
  • nucleolus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Serine/arginine-rich splicing factor 5PRO_0000081928Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei167 – 1671N6-acetyllysineCombined sources
Modified residuei224 – 2241PhosphoserineBy similarity
Modified residuei226 – 2261PhosphoserineBy similarity
Modified residuei230 – 2301PhosphoserineBy similarity
Modified residuei247 – 2471PhosphoserineBy similarity
Modified residuei250 – 2501PhosphoserineBy similarity

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35326.
PaxDbiO35326.
PRIDEiO35326.

PTM databases

iPTMnetiO35326.
PhosphoSiteiO35326.

Expressioni

Gene expression databases

ExpressionAtlasiO35326. baseline and differential.
GenevisibleiO35326. MM.

Interactioni

Subunit structurei

Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2 (By similarity). Interacts (via RS domain) with PHF5A (via N-terminus).By similarity1 Publication

GO - Molecular functioni

  • RS domain binding Source: MGI

Protein-protein interaction databases

BioGridi203190. 1 interaction.
DIPiDIP-48724N.
STRINGi10090.ENSMUSP00000105981.

Structurei

3D structure databases

ProteinModelPortaliO35326.
SMRiO35326. Positions 4-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 7471RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini108 – 18982RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi78 – 836Gly-rich (hinge region)
Compositional biasi190 – 26475Arg/Ser-rich (RS domain)Add
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104103.
HOGENOMiHOG000276234.
HOVERGENiHBG002295.
InParanoidiO35326.
KOiK12893.
OrthoDBiEOG7H1JPR.
TreeFamiTF351335.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGCRVFIGR LNPAAREKDV ERFFKGYGRI RDIDLKRGFG FVEFEDPRDA
60 70 80 90 100
DDAVYELDGK ELCSERVTIE HARARSRGGR GRGRYSDRFS SRRPRNDRRN
110 120 130 140 150
APPVRTENRL IVENLSSRVS WQDLKDFMRQ AGEVTFADAH RPKLNEGVVE
160 170 180 190 200
FASYGDLKNA IEKLSGKEIN GRKIKLIEGS KRHRSRSRSR SRTRSSSRSR
210 220 230 240 250
SRSRSRRSKS YSRSRSRSRS RSKSRSGSRS PVPEKSQKRG SSSRSKSPAS
260
VDRQRSRSRS RSRSVDSGN
Length:269
Mass (Da):30,891
Last modified:October 31, 2012 - v2
Checksum:i0FC90628B64DE845
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020308 Genomic DNA. Translation: AAC39946.1.
AK146624 mRNA. Translation: BAE27313.1.
AC125092 Genomic DNA. No translation available.
CH466590 Genomic DNA. Translation: EDL02685.1.
CH466590 Genomic DNA. Translation: EDL02687.1.
CH466590 Genomic DNA. Translation: EDL02690.1.
CH466590 Genomic DNA. Translation: EDL02692.1.
CH466590 Genomic DNA. Translation: EDL02693.1.
BC082593 mRNA. Translation: AAH82593.1.
CCDSiCCDS36484.1.
RefSeqiNP_001073162.1. NM_001079694.1.
NP_001073163.1. NM_001079695.1.
NP_033185.2. NM_009159.2.
UniGeneiMm.43331.

Genome annotation databases

EnsembliENSMUST00000094693; ENSMUSP00000131323; ENSMUSG00000021134.
ENSMUST00000110352; ENSMUSP00000105981; ENSMUSG00000021134.
ENSMUST00000110356; ENSMUSP00000105985; ENSMUSG00000021134.
GeneIDi20384.
KEGGimmu:20384.
UCSCiuc007obo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020308 Genomic DNA. Translation: AAC39946.1.
AK146624 mRNA. Translation: BAE27313.1.
AC125092 Genomic DNA. No translation available.
CH466590 Genomic DNA. Translation: EDL02685.1.
CH466590 Genomic DNA. Translation: EDL02687.1.
CH466590 Genomic DNA. Translation: EDL02690.1.
CH466590 Genomic DNA. Translation: EDL02692.1.
CH466590 Genomic DNA. Translation: EDL02693.1.
BC082593 mRNA. Translation: AAH82593.1.
CCDSiCCDS36484.1.
RefSeqiNP_001073162.1. NM_001079694.1.
NP_001073163.1. NM_001079695.1.
NP_033185.2. NM_009159.2.
UniGeneiMm.43331.

3D structure databases

ProteinModelPortaliO35326.
SMRiO35326. Positions 4-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203190. 1 interaction.
DIPiDIP-48724N.
STRINGi10090.ENSMUSP00000105981.

PTM databases

iPTMnetiO35326.
PhosphoSiteiO35326.

Proteomic databases

EPDiO35326.
PaxDbiO35326.
PRIDEiO35326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094693; ENSMUSP00000131323; ENSMUSG00000021134.
ENSMUST00000110352; ENSMUSP00000105981; ENSMUSG00000021134.
ENSMUST00000110356; ENSMUSP00000105985; ENSMUSG00000021134.
GeneIDi20384.
KEGGimmu:20384.
UCSCiuc007obo.1. mouse.

Organism-specific databases

CTDi6430.
MGIiMGI:98287. Srsf5.

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104103.
HOGENOMiHOG000276234.
HOVERGENiHBG002295.
InParanoidiO35326.
KOiK12893.
OrthoDBiEOG7H1JPR.
TreeFamiTF351335.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.

Miscellaneous databases

ChiTaRSiSrsf5. mouse.
NextBioi298306.
PROiO35326.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiO35326. baseline and differential.
GenevisibleiO35326. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Alternative splicing and structure of the human and mouse SFRS5/HRS/SRp40 genes."
    Du K., Taub R.
    Gene 204:243-249(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Amnion.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  6. "PHF5A represents a bridge protein between splicing proteins and ATP-dependent helicases and is differentially expressed during mouse spermatogenesis."
    Rzymski T., Grzmil P., Meinhardt A., Wolf S., Burfeind P.
    Cytogenet. Genome Res. 121:232-244(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PHF5A.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-167, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSRSF5_MOUSE
AccessioniPrimary (citable) accession number: O35326
Secondary accession number(s): Q640L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 31, 2012
Last modified: March 16, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.