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Reviewed, UniProtKB/Swiss-Prot O35304 (VACHT_MOUSE)

Last modified October 13, 2009. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Vesicular acetylcholine transporter
      Short name=VAChT
Alternative name(s):
    Solute carrier family 18 member 3
Gene names
Name: Slc18a3
Synonyms: Vacht
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Involved in acetylcholine transport into synaptic vesicles By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in the spinal cord, brain (excluding the cerebellum), brain stem and cholinergic tissues. Not expressed in peripheral tissues such as liver and kidney. Ref.1

Sequence similarities

Belongs to the major facilitator superfamily. Vesicular transporter family.

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Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processneurotransmitter transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

terminal button

Inferred from direct assay. Source: MGI

   Molecular functionmonoamine transmembrane transporter activity Ref.1

Traceable author statement. Source: MGI

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 530530Vesicular acetylcholine transporter
PRO_0000127519

Regions

Topological domain1 – 3333Cytoplasmic Potential
Transmembrane34 – 5421 Potential
Topological domain55 – 12571Lumenal, vesicle Potential
Transmembrane126 – 14621 Potential
Topological domain147 – 1526Cytoplasmic Potential
Transmembrane153 – 17321 Potential
Topological domain174 – 1829Lumenal, vesicle Potential
Transmembrane183 – 20321 Potential
Topological domain204 – 21310Cytoplasmic Potential
Transmembrane214 – 23421 Potential
Topological domain235 – 2428Lumenal, vesicle Potential
Transmembrane243 – 26321 Potential
Topological domain264 – 28825Cytoplasmic Potential
Transmembrane289 – 30921 Potential
Topological domain310 – 32516Lumenal, vesicle Potential
Transmembrane326 – 34621 Potential
Topological domain347 – 35610Cytoplasmic Potential
Transmembrane357 – 37721 Potential
Topological domain378 – 38811Lumenal, vesicle Potential
Transmembrane389 – 40921 Potential
Topological domain410 – 42213Cytoplasmic Potential
Transmembrane423 – 44321 Potential
Topological domain444 – 4474Lumenal, vesicle Potential
Transmembrane448 – 46821 Potential
Topological domain469 – 53062Cytoplasmic Potential

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
O35304-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 08526F29B4E8EEDD

FASTA53056,615
        10         20         30         40         50         60 
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY 

        70         80         90        100        110        120 
IAHMRGGSES PTLISEVWEP TLPPPTLANA SAYLANTSAS PTAAGSARSI LRPRYPTESE 

       130        140        150        160        170        180 
DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVMFA FAEDYATFFA 

       190        200        210        220        230        240 
ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG 

       250        260        270        280        290        300 
KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT 

       310        320        330        340        350        360 
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG 

       370        380        390        400        410        420 
ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV 

       430        440        450        460        470        480 
YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS 

       490        500        510        520        530 
ERDVLLDEPP QGLYDAVRLR EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS

« Hide

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References

[1]"Molecular characterization of the mouse vesicular acetylcholine transporter gene."
Naciff J.M., Misawa H., Dedman J.R.
NeuroReport 8:3467-3473(1997) [PubMed: 9427309] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: BALB/c.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF019045 Genomic DNA. Translation: AAD09151.1.
IPIIPI00129630.
UniGeneMm.190503

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteO35304.

Proteomic databases

PRIDEO35304.

Organism-specific databases

MGIMGI:1101061. Slc18a3.

Phylogenomic databases

HOVERGENO35304.

Gene expression databases

GenevestigatorO35304.

Family and domain databases

InterProIPR011701. MFS_1.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameVACHT_MOUSE
AccessionPrimary (citable) accession number: O35304
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: January 1, 1998
Last modified: October 13, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents