O35304 (VACHT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicular acetylcholine transporter
Short name=VAChT

Alternative name(s):
Solute carrier family 18 member 3

Gene names

Name:	Slc18a3
Synonyms:	Vacht

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in acetylcholine transport into synaptic vesicles By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in the spinal cord, brain (excluding the cerebellum), brain stem and cholinergic tissues. Not expressed in peripheral tissues such as liver and kidney. Ref.1
Sequence similarities	Belongs to the major facilitator superfamily. Vesicular transporter family.

Ontologies

Keywords
Biological process	Neurotransmitter transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	clathrin sculpted acetylcholine transport vesicle membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetylcholine transmembrane transporter activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Vesicular acetylcholine transporter		PRO_0000127519
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 54	21	Helical; Potential
	Topological domain	55 – 125	71	Lumenal, vesicle Potential
	Transmembrane	126 – 146	21	Helical; Potential
	Topological domain	147 – 152	6	Cytoplasmic Potential
	Transmembrane	153 – 173	21	Helical; Potential
	Topological domain	174 – 182	9	Lumenal, vesicle Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Topological domain	204 – 213	10	Cytoplasmic Potential
	Transmembrane	214 – 234	21	Helical; Potential
	Topological domain	235 – 242	8	Lumenal, vesicle Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Topological domain	264 – 288	25	Cytoplasmic Potential
	Transmembrane	289 – 309	21	Helical; Potential
	Topological domain	310 – 325	16	Lumenal, vesicle Potential
	Transmembrane	326 – 346	21	Helical; Potential
	Topological domain	347 – 356	10	Cytoplasmic Potential
	Transmembrane	357 – 377	21	Helical; Potential
	Topological domain	378 – 388	11	Lumenal, vesicle Potential
	Transmembrane	389 – 409	21	Helical; Potential
	Topological domain	410 – 422	13	Cytoplasmic Potential
	Transmembrane	423 – 443	21	Helical; Potential
	Topological domain	444 – 447	4	Lumenal, vesicle Potential
	Transmembrane	448 – 468	21	Helical; Potential
	Topological domain	469 – 530	62	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	96	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O35304 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 08526F29B4E8EEDD	FASTA	530	56,615
	10 20 30 40 50 60 MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY 70 80 90 100 110 120 IAHMRGGSES PTLISEVWEP TLPPPTLANA SAYLANTSAS PTAAGSARSI LRPRYPTESE 130 140 150 160 170 180 DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVMFA FAEDYATFFA 190 200 210 220 230 240 ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG 250 260 270 280 290 300 KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT 310 320 330 340 350 360 CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG 370 380 390 400 410 420 ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV 430 440 450 460 470 480 YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS 490 500 510 520 530 ERDVLLDEPP QGLYDAVRLR EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS

References

[1]	"Molecular characterization of the mouse vesicular acetylcholine transporter gene." Naciff J.M., Misawa H., Dedman J.R. NeuroReport 8:3467-3473(1997) [PubMed: 9427309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019045 Genomic DNA. Translation: AAD09151.1.
IPI	IPI00129630.
UniGene	Mm.190503.
3D structure databases
ProteinModelPortal	O35304.
ModBase	Search...
PTM databases
PhosphoSite	O35304.
Proteomic databases
PRIDE	O35304.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:1101061. Slc18a3.
Phylogenomic databases
HOVERGEN	HBG055082.
Gene expression databases
Genevestigator	O35304.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

VACHT_MOUSE

Accession

Primary (citable) accession number: O35304

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	January 1, 1998
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program