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Protein

Vesicular acetylcholine transporter

Gene

Slc18a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in acetylcholine transport into synaptic vesicles.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular acetylcholine transporter
Short name:
VAChT
Alternative name(s):
Solute carrier family 18 member 3
Gene namesi
Name:Slc18a3
Synonyms:Vacht
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1101061. Slc18a3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 125Lumenal, vesicleSequence analysisAdd BLAST71
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 152CytoplasmicSequence analysis6
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 182Lumenal, vesicleSequence analysis9
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 213CytoplasmicSequence analysis10
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Topological domaini235 – 242Lumenal, vesicleSequence analysis8
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 288CytoplasmicSequence analysisAdd BLAST25
Transmembranei289 – 309HelicalSequence analysisAdd BLAST21
Topological domaini310 – 325Lumenal, vesicleSequence analysisAdd BLAST16
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Topological domaini347 – 356CytoplasmicSequence analysis10
Transmembranei357 – 377HelicalSequence analysisAdd BLAST21
Topological domaini378 – 388Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 422CytoplasmicSequence analysisAdd BLAST13
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 447Lumenal, vesicleSequence analysis4
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 530CytoplasmicSequence analysisAdd BLAST62

GO - Cellular componenti

  • AP-1 adaptor complex Source: GO_Central
  • AP-2 adaptor complex Source: GO_Central
  • clathrin-sculpted acetylcholine transport vesicle membrane Source: UniProtKB
  • cytoplasm Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • neuron projection Source: MGI
  • synaptic vesicle membrane Source: GO_Central
  • terminal bouton Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275191 – 530Vesicular acetylcholine transporterAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO35304.

PTM databases

iPTMnetiO35304.
PhosphoSitePlusiO35304.

Expressioni

Tissue specificityi

Expressed in the spinal cord, brain (excluding the cerebellum), brain stem and cholinergic tissues. Not expressed in peripheral tissues such as liver and kidney.1 Publication

Interactioni

Subunit structurei

Interacts with SEC14L1.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO35304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni471 – 530Mediates interaction with SEC14L11 PublicationAdd BLAST60

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG055082.
InParanoidiO35304.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35304-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY
60 70 80 90 100
MVIVPIVPDY IAHMRGGSES PTLISEVWEP TLPPPTLANA SAYLANTSAS
110 120 130 140 150
PTAAGSARSI LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS
160 170 180 190 200
YDVPLLIGLG VMFASTVMFA FAEDYATFFA ARSLQGLGSA FADTSGIAMI
210 220 230 240 250
ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA
260 270 280 290 300
VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT
310 320 330 340 350
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA
360 370 380 390 400
RYPHLQWLYG ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA
410 420 430 440 450
LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ
460 470 480 490 500
LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR
510 520 530
EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS
Length:530
Mass (Da):56,615
Last modified:January 1, 1998 - v1
Checksum:i08526F29B4E8EEDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019045 Genomic DNA. Translation: AAD09151.1.
CCDSiCCDS79291.1.
UniGeneiMm.190503.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019045 Genomic DNA. Translation: AAD09151.1.
CCDSiCCDS79291.1.
UniGeneiMm.190503.

3D structure databases

ProteinModelPortaliO35304.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiO35304.
PhosphoSitePlusiO35304.

Proteomic databases

PRIDEiO35304.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1101061. Slc18a3.

Phylogenomic databases

HOVERGENiHBG055082.
InParanoidiO35304.

Miscellaneous databases

PROiO35304.
SOURCEiSearch...

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVACHT_MOUSE
AccessioniPrimary (citable) accession number: O35304
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.