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Protein

Transcriptional activator protein Pur-beta

Gene

Purb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity). Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts.By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi37 – 263Add BLAST227

GO - Molecular functioni

  • DNA binding transcription factor activity Source: MGI
  • double-stranded DNA binding Source: MGI
  • double-stranded telomeric DNA binding Source: UniProtKB
  • mRNA binding Source: UniProtKB
  • RNA binding Source: MGI
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • single-stranded DNA binding Source: UniProtKB
  • SMAD binding Source: MGI
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • cell differentiation Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • DNA unwinding involved in DNA replication Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: NTNU_SB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein Pur-beta
Alternative name(s):
Purine-rich element-binding protein B
Vascular actin single-stranded DNA-binding factor 2 p44 component
Gene namesi
Name:Purb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1338779 Purb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002256162 – 324Transcriptional activator protein Pur-betaAdd BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei8PhosphoserineCombined sources1
Modified residuei28Omega-N-methylarginineCombined sources1
Modified residuei43PhosphothreonineCombined sources1
Modified residuei113PhosphoserineCombined sources1
Modified residuei164Omega-N-methylarginineCombined sources1
Modified residuei279N6-acetyllysineCombined sources1
Modified residuei306Omega-N-methylarginineCombined sources1
Modified residuei310PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO35295
MaxQBiO35295
PaxDbiO35295
PeptideAtlasiO35295
PRIDEiO35295

PTM databases

iPTMnetiO35295
PhosphoSitePlusiO35295

Expressioni

Gene expression databases

BgeeiENSMUSG00000094483
CleanExiMM_PURB
GenevisibleiO35295 MM

Interactioni

Subunit structurei

Homodimer, heterodimer with PURA and heterotrimer with PURA and YBX1/Y-box protein 1.1 Publication

GO - Molecular functioni

  • SMAD binding Source: MGI
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi202516, 5 interactors
IntActiO35295, 8 interactors
MINTiO35295
STRINGi10090.ENSMUSP00000136957

Structurei

3D structure databases

ProteinModelPortaliO35295
SMRiO35295
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 21Poly-GlyAdd BLAST11
Compositional biasi29 – 39Poly-GlyAdd BLAST11
Compositional biasi165 – 234Gly-richAdd BLAST70

Sequence similaritiesi

Belongs to the PUR DNA-binding protein family.Curated

Phylogenomic databases

eggNOGiKOG3074 Eukaryota
ENOG410ZKKR LUCA
GeneTreeiENSGT00390000015406
HOGENOMiHOG000232132
HOVERGENiHBG006888
InParanoidiO35295
KOiK21799
OMAiFQPAPRG
OrthoDBiEOG091G0JS7
PhylomeDBiO35295
TreeFamiTF313701

Family and domain databases

InterProiView protein in InterPro
IPR006628 PUR-bd_fam
IPR030499 PURbeta
PANTHERiPTHR12611 PTHR12611, 1 hit
PTHR12611:SF4 PTHR12611:SF4, 1 hit
PfamiView protein in Pfam
PF04845 PurA, 1 hit
SMARTiView protein in SMART
SM00712 PUR, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGDSGSER GGGGGGGGGP GGFQPAPRGG GGGGGGPGGE QETQELASKR
60 70 80 90 100
LDIQNKRFYL DVKQNAKGRF LKIAEVGAGG SKSRLTLSMA VAAEFRDSLG
110 120 130 140 150
DFIEHYAQLG PSSPEQLAAG AEEGGGPRRA LKSEFLVREN RKYYLDLKEN
160 170 180 190 200
QRGRFLRIRQ TVNRGGGGFG GGPGPGGLQS GQTIALPAQG LIEFRDALAK
210 220 230 240 250
LIDDYGGDED ELAGGPGGGA GGPGGGLYGE LPEGTSITVD SKRFFFDVGC
260 270 280 290 300
NKYGVFLRVS EVKPSYRNAI TVPFKAWGKF GGAFCRYADE MKEIQERQRD
310 320
KLYERRGGGS GGGDESEGEE VDED
Length:324
Mass (Da):33,901
Last modified:January 23, 2007 - v3
Checksum:i5CB70CCE3FDB7913
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017630 mRNA Translation: AAB71859.1
AL646020 Genomic DNA No translation available.
CCDSiCCDS24420.1
RefSeqiNP_035351.1, NM_011221.3
UniGeneiMm.296150
Mm.390332
Mm.474938

Genome annotation databases

EnsembliENSMUST00000179343; ENSMUSP00000136957; ENSMUSG00000094483
GeneIDi19291
KEGGimmu:19291
UCSCiuc007hyr.3 mouse

Similar proteinsi

Entry informationi

Entry nameiPURB_MOUSE
AccessioniPrimary (citable) accession number: O35295
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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