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Protein

Transcriptional activator protein Pur-beta

Gene

Purb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity). Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts.By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi37 – 263Add BLAST227

GO - Molecular functioni

  • double-stranded DNA binding Source: MGI
  • double-stranded telomeric DNA binding Source: UniProtKB
  • mRNA binding Source: UniProtKB
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • single-stranded DNA binding Source: UniProtKB
  • SMAD binding Source: MGI
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • cell differentiation Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • DNA unwinding involved in DNA replication Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein Pur-beta
Alternative name(s):
Purine-rich element-binding protein B
Vascular actin single-stranded DNA-binding factor 2 p44 component
Gene namesi
Name:Purb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1338779. Purb.

Subcellular locationi

GO - Cellular componenti

  • DNA replication factor A complex Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002256162 – 324Transcriptional activator protein Pur-betaAdd BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei8PhosphoserineCombined sources1
Modified residuei28Omega-N-methylarginineCombined sources1
Modified residuei43PhosphothreonineCombined sources1
Modified residuei113PhosphoserineCombined sources1
Modified residuei164Omega-N-methylarginineCombined sources1
Modified residuei279N6-acetyllysineCombined sources1
Modified residuei306Omega-N-methylarginineCombined sources1
Modified residuei310PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO35295.
MaxQBiO35295.
PaxDbiO35295.
PeptideAtlasiO35295.
PRIDEiO35295.

PTM databases

iPTMnetiO35295.
PhosphoSitePlusiO35295.

Expressioni

Gene expression databases

BgeeiENSMUSG00000094483.
CleanExiMM_PURB.
GenevisibleiO35295. MM.

Interactioni

Subunit structurei

Homodimer, heterodimer with PURA and heterotrimer with PURA and YBX1/Y-box protein 1.1 Publication

GO - Molecular functioni

  • SMAD binding Source: MGI
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi202516. 2 interactors.
IntActiO35295. 6 interactors.
MINTiMINT-4104684.
STRINGi10090.ENSMUSP00000136957.

Structurei

3D structure databases

ProteinModelPortaliO35295.
SMRiO35295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 21Poly-GlyAdd BLAST11
Compositional biasi29 – 39Poly-GlyAdd BLAST11
Compositional biasi165 – 234Gly-richAdd BLAST70

Sequence similaritiesi

Belongs to the PUR DNA-binding protein family.Curated

Phylogenomic databases

eggNOGiKOG3074. Eukaryota.
ENOG410ZKKR. LUCA.
GeneTreeiENSGT00390000015406.
HOGENOMiHOG000232132.
HOVERGENiHBG006888.
InParanoidiO35295.
OMAiENQRGRY.
OrthoDBiEOG091G0JS7.
PhylomeDBiO35295.
TreeFamiTF313701.

Family and domain databases

InterProiIPR006628. PUR-bd_fam.
IPR030499. PURbeta.
[Graphical view]
PANTHERiPTHR12611. PTHR12611. 1 hit.
PTHR12611:SF4. PTHR12611:SF4. 1 hit.
PfamiPF04845. PurA. 1 hit.
[Graphical view]
SMARTiSM00712. PUR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGDSGSER GGGGGGGGGP GGFQPAPRGG GGGGGGPGGE QETQELASKR
60 70 80 90 100
LDIQNKRFYL DVKQNAKGRF LKIAEVGAGG SKSRLTLSMA VAAEFRDSLG
110 120 130 140 150
DFIEHYAQLG PSSPEQLAAG AEEGGGPRRA LKSEFLVREN RKYYLDLKEN
160 170 180 190 200
QRGRFLRIRQ TVNRGGGGFG GGPGPGGLQS GQTIALPAQG LIEFRDALAK
210 220 230 240 250
LIDDYGGDED ELAGGPGGGA GGPGGGLYGE LPEGTSITVD SKRFFFDVGC
260 270 280 290 300
NKYGVFLRVS EVKPSYRNAI TVPFKAWGKF GGAFCRYADE MKEIQERQRD
310 320
KLYERRGGGS GGGDESEGEE VDED
Length:324
Mass (Da):33,901
Last modified:January 23, 2007 - v3
Checksum:i5CB70CCE3FDB7913
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017630 mRNA. Translation: AAB71859.1.
AL646020 Genomic DNA. Translation: CAI26011.1.
CCDSiCCDS24420.1.
RefSeqiNP_035351.1. NM_011221.3.
UniGeneiMm.296150.
Mm.390332.
Mm.474938.

Genome annotation databases

EnsembliENSMUST00000179343; ENSMUSP00000136957; ENSMUSG00000094483.
GeneIDi19291.
KEGGimmu:19291.
UCSCiuc007hyr.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017630 mRNA. Translation: AAB71859.1.
AL646020 Genomic DNA. Translation: CAI26011.1.
CCDSiCCDS24420.1.
RefSeqiNP_035351.1. NM_011221.3.
UniGeneiMm.296150.
Mm.390332.
Mm.474938.

3D structure databases

ProteinModelPortaliO35295.
SMRiO35295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202516. 2 interactors.
IntActiO35295. 6 interactors.
MINTiMINT-4104684.
STRINGi10090.ENSMUSP00000136957.

PTM databases

iPTMnetiO35295.
PhosphoSitePlusiO35295.

Proteomic databases

EPDiO35295.
MaxQBiO35295.
PaxDbiO35295.
PeptideAtlasiO35295.
PRIDEiO35295.

Protocols and materials databases

DNASUi19291.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000179343; ENSMUSP00000136957; ENSMUSG00000094483.
GeneIDi19291.
KEGGimmu:19291.
UCSCiuc007hyr.3. mouse.

Organism-specific databases

CTDi5814.
MGIiMGI:1338779. Purb.

Phylogenomic databases

eggNOGiKOG3074. Eukaryota.
ENOG410ZKKR. LUCA.
GeneTreeiENSGT00390000015406.
HOGENOMiHOG000232132.
HOVERGENiHBG006888.
InParanoidiO35295.
OMAiENQRGRY.
OrthoDBiEOG091G0JS7.
PhylomeDBiO35295.
TreeFamiTF313701.

Miscellaneous databases

ChiTaRSiPurb. mouse.
PROiO35295.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000094483.
CleanExiMM_PURB.
GenevisibleiO35295. MM.

Family and domain databases

InterProiIPR006628. PUR-bd_fam.
IPR030499. PURbeta.
[Graphical view]
PANTHERiPTHR12611. PTHR12611. 1 hit.
PTHR12611:SF4. PTHR12611:SF4. 1 hit.
PfamiPF04845. PurA. 1 hit.
[Graphical view]
SMARTiSM00712. PUR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPURB_MOUSE
AccessioniPrimary (citable) accession number: O35295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.