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Protein

Eosinophil cationic-type ribonuclease 5

Gene

Ear5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331SubstrateBy similarity
Active sitei38 – 381Proton acceptorBy similarity
Binding sitei87 – 871SubstrateBy similarity
Active sitei150 – 1501Proton donorBy similarity

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Eosinophil cationic-type ribonuclease 5
Alternative name(s):
MR-5
Gene namesi
Name:Ear5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:1858598. Ear5.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 155130Eosinophil cationic-type ribonuclease 5PRO_0000030870Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 106By similarity
Disulfide bondi61 ↔ 118By similarity
Disulfide bondi79 ↔ 133By similarity
Disulfide bondi86 ↔ 94By similarity
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Nitration

Proteomic databases

PRIDEiO35292.

PTM databases

PhosphoSiteiO35292.

Expressioni

Gene expression databases

ExpressionAtlasiO35292. differential.
GenevestigatoriO35292.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075074.

Structurei

3D structure databases

ProteinModelPortaliO35292.
SMRiO35292. Positions 29-154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 665Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG39501.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiO35292.
OMAiPKCDDAM.
OrthoDBiEOG7KDFCP.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35292-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLKLLESRL CLLLLLGLVL TLVSCQRPTP SQKFDIQHIY KKSSPKCDDA
60 70 80 90 100
MRVVNKYTGK CKDLNTFLHT TFADVVRVCH NPPKTCKDGT SPNCHDSSSK
110 120 130 140 150
VSVTICKLTK RARNYTHCRY KTTGAKKSYT VACNPRTPKD RPTYPVVPVH

LDRLF
Length:155
Mass (Da):17,514
Last modified:October 3, 2012 - v2
Checksum:iE22D48C15634065A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351D → A in AAC53491. (PubMed:9336452)Curated
Sequence conflicti125 – 1251A → G in AAC53491. (PubMed:9336452)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017260 Genomic DNA. Translation: AAC53491.1.
AF512013 Genomic DNA. Translation: AAM44856.1.
AC163664 Genomic DNA. No translation available.
CH466605 Genomic DNA. Translation: EDL20848.1.
BC117776 mRNA. Translation: AAI17777.1.
BC119506 mRNA. Translation: AAI19507.1.
CCDSiCCDS27039.1.
RefSeqiNP_062271.2. NM_019398.2.
UniGeneiMm.377125.

Genome annotation databases

EnsembliENSMUST00000075648; ENSMUSP00000075074; ENSMUSG00000059606.
GeneIDi54159.
KEGGimmu:54159.
UCSCiuc007tmt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017260 Genomic DNA. Translation: AAC53491.1.
AF512013 Genomic DNA. Translation: AAM44856.1.
AC163664 Genomic DNA. No translation available.
CH466605 Genomic DNA. Translation: EDL20848.1.
BC117776 mRNA. Translation: AAI17777.1.
BC119506 mRNA. Translation: AAI19507.1.
CCDSiCCDS27039.1.
RefSeqiNP_062271.2. NM_019398.2.
UniGeneiMm.377125.

3D structure databases

ProteinModelPortaliO35292.
SMRiO35292. Positions 29-154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075074.

PTM databases

PhosphoSiteiO35292.

Proteomic databases

PRIDEiO35292.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075648; ENSMUSP00000075074; ENSMUSG00000059606.
GeneIDi54159.
KEGGimmu:54159.
UCSCiuc007tmt.2. mouse.

Organism-specific databases

CTDi54159.
MGIiMGI:1858598. Ear5.

Phylogenomic databases

eggNOGiNOG39501.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiO35292.
OMAiPKCDDAM.
OrthoDBiEOG7KDFCP.
TreeFamiTF333393.

Miscellaneous databases

NextBioi310996.
PROiO35292.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiO35292. differential.
GenevestigatoriO35292.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of four novel murine ribonuclease genes: unusual expansion within the ribonuclease A gene family."
    Batten D., Dyer K.D., Domachowske J.B., Rosenberg H.F.
    Nucleic Acids Res. 25:4235-4239(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Fibroblast.
  2. "Transcriptional activation of the mouse eosinophil-associated ribonuclease 2 (Ear2) gene by an intronic enhancer element that binds NFATc1."
    Moreau J.M., McDevitt A.L., Rosenberg H.F., Dyer K.D.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NITRATION.

Entry informationi

Entry nameiECP5_MOUSE
AccessioniPrimary (citable) accession number: O35292
Secondary accession number(s): Q8K416
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 3, 2012
Last modified: February 4, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.