O35292 (ECP5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eosinophil cationic-type ribonuclease 5

Alternative name(s):
MR-5

Gene names

Name:

Ear5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein Nitration
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 155	130	Eosinophil cationic-type ribonuclease 5		PRO_0000030870
Regions
	Region	62 – 66	5	Substrate binding By similarity
Sites
	Active site	38	1	Proton acceptor By similarity
	Active site	150	1	Proton donor By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
Amino acid modifications
	Modified residue	57	1	Nitrated tyrosine By similarity
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 106		By similarity
	Disulfide bond	61 ↔ 118		By similarity
	Disulfide bond	79 ↔ 133		By similarity
	Disulfide bond	86 ↔ 94		By similarity
Experimental info
	Sequence conflict	35	1	D → A in AAC53491. Ref.1
	Sequence conflict	125	1	A → G in AAC53491. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O35292 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: E22D48C15634065A	FASTA	155	17,514
	10 20 30 40 50 60 MGLKLLESRL CLLLLLGLVL TLVSCQRPTP SQKFDIQHIY KKSSPKCDDA MRVVNKYTGK 70 80 90 100 110 120 CKDLNTFLHT TFADVVRVCH NPPKTCKDGT SPNCHDSSSK VSVTICKLTK RARNYTHCRY 130 140 150 KTTGAKKSYT VACNPRTPKD RPTYPVVPVH LDRLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of four novel murine ribonuclease genes: unusual expansion within the ribonuclease A gene family." Batten D., Dyer K.D., Domachowske J.B., Rosenberg H.F. Nucleic Acids Res. 25:4235-4239(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Fibroblast.
[2]	"Transcriptional activation of the mouse eosinophil-associated ribonuclease 2 (Ear2) gene by an intronic enhancer element that binds NFATc1." Moreau J.M., McDevitt A.L., Rosenberg H.F., Dyer K.D. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase." Ulrich M., Petre A., Youhnovski N., Proemm F., Schirle M., Schumm M., Pero R.S., Doyle A., Checkel J., Kita H., Thiyagarajan N., Acharya K.R., Schmid-Grendelmeier P., Simon H.-U., Schwarz H., Tsutsui M., Shimokawa H., Bellon G. , Lee J.J., Przybylski M., Doering G. J. Biol. Chem. 283:28629-28640(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NITRATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017260 Genomic DNA. Translation: AAC53491.1. AF512013 Genomic DNA. Translation: AAM44856.1. AC163664 Genomic DNA. No translation available. CH466605 Genomic DNA. Translation: EDL20848.1. BC117776 mRNA. Translation: AAI17777.1. BC119506 mRNA. Translation: AAI19507.1.
IPI	IPI00128866. IPI00944128.
RefSeq	NP_062271.2. NM_019398.2.
UniGene	Mm.377125.
3D structure databases
ProteinModelPortal	O35292.
SMR	O35292. Positions 29-154.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000075074.
PTM databases
PhosphoSite	O35292.
Proteomic databases
PRIDE	O35292.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000075648; ENSMUSP00000075074; ENSMUSG00000059606.
GeneID	54159.
KEGG	mmu:54159.
Organism-specific databases
CTD	54159.
MGI	MGI:1858598. Ear5.
Phylogenomic databases
eggNOG	NOG39501.
GeneTree	ENSGT00390000007840.
HOGENOM	HOG000276882.
HOVERGEN	HBG008396.
InParanoid	O35292.
OMA	PKCDDAM.
OrthoDB	EOG4GXFP0.
Gene expression databases
Genevestigator	O35292.
GermOnline	ENSMUSG00000059606. Mus musculus.
Family and domain databases
Gene3D	3.10.130.10. 1 hit.
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
PANTHER	PTHR11437. PTHR11437. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	310996.
SOURCE	Search...

Entry information

Entry name

ECP5_MOUSE

Accession

Primary (citable) accession number: O35292
Secondary accession number(s): Q8K416

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program