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Protein

Ribonuclease 2B

Gene

Rnase2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38Proton acceptorBy similarity1
Active sitei150Proton donorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 2BImported (EC:3.1.27.5By similarity)
Alternative name(s):
Eosinophil cationic-type ribonuclease 5Curated
MR-5Curated
Gene namesi
Name:Rnase2bImported
Synonyms:Ear5Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1858598. Rnase2b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000003087026 – 155Ribonuclease 2BCuratedAdd BLAST130

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 106By similarity
Disulfide bondi61 ↔ 118By similarity
Disulfide bondi79 ↔ 133By similarity
Disulfide bondi86 ↔ 94By similarity
Glycosylationi114N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Nitration

Proteomic databases

PaxDbiO35292.
PRIDEiO35292.

PTM databases

iPTMnetiO35292.
PhosphoSitePlusiO35292.

Expressioni

Gene expression databases

BgeeiENSMUSG00000059606.
ExpressionAtlasiO35292. baseline and differential.
GenevisibleiO35292. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075074.

Structurei

3D structure databases

ProteinModelPortaliO35292.
SMRiO35292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 66Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JDT3. Eukaryota.
ENOG411136R. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiO35292.
OMAiNQSIRCP.
OrthoDBiEOG091G0SFK.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35292-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLKLLESRL CLLLLLGLVL TLVSCQRPTP SQKFDIQHIY KKSSPKCDDA
60 70 80 90 100
MRVVNKYTGK CKDLNTFLHT TFADVVRVCH NPPKTCKDGT SPNCHDSSSK
110 120 130 140 150
VSVTICKLTK RARNYTHCRY KTTGAKKSYT VACNPRTPKD RPTYPVVPVH

LDRLF
Length:155
Mass (Da):17,514
Last modified:October 3, 2012 - v2
Checksum:iE22D48C15634065A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35D → A in AAC53491 (PubMed:9336452).Curated1
Sequence conflicti125A → G in AAC53491 (PubMed:9336452).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017260 Genomic DNA. Translation: AAC53491.1.
AF512013 Genomic DNA. Translation: AAM44856.1.
AC163664 Genomic DNA. No translation available.
CH466605 Genomic DNA. Translation: EDL20848.1.
BC117776 mRNA. Translation: AAI17777.1.
BC119506 mRNA. Translation: AAI19507.1.
CCDSiCCDS27039.1.
RefSeqiNP_062271.2. NM_019398.2.
UniGeneiMm.377125.

Genome annotation databases

EnsembliENSMUST00000075648; ENSMUSP00000075074; ENSMUSG00000059606.
GeneIDi54159.
KEGGimmu:54159.
UCSCiuc007tmt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017260 Genomic DNA. Translation: AAC53491.1.
AF512013 Genomic DNA. Translation: AAM44856.1.
AC163664 Genomic DNA. No translation available.
CH466605 Genomic DNA. Translation: EDL20848.1.
BC117776 mRNA. Translation: AAI17777.1.
BC119506 mRNA. Translation: AAI19507.1.
CCDSiCCDS27039.1.
RefSeqiNP_062271.2. NM_019398.2.
UniGeneiMm.377125.

3D structure databases

ProteinModelPortaliO35292.
SMRiO35292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075074.

PTM databases

iPTMnetiO35292.
PhosphoSitePlusiO35292.

Proteomic databases

PaxDbiO35292.
PRIDEiO35292.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075648; ENSMUSP00000075074; ENSMUSG00000059606.
GeneIDi54159.
KEGGimmu:54159.
UCSCiuc007tmt.2. mouse.

Organism-specific databases

CTDi54159.
MGIiMGI:1858598. Rnase2b.

Phylogenomic databases

eggNOGiENOG410JDT3. Eukaryota.
ENOG411136R. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiO35292.
OMAiNQSIRCP.
OrthoDBiEOG091G0SFK.
TreeFamiTF333393.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-6803157. Antimicrobial peptides.

Miscellaneous databases

PROiO35292.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059606.
ExpressionAtlasiO35292. baseline and differential.
GenevisibleiO35292. MM.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNS2B_MOUSE
AccessioniPrimary (citable) accession number: O35292
Secondary accession number(s): Q8K416
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.