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O35291

- RNAS2_MOUSE

UniProt

O35291 - RNAS2_MOUSE

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Protein
Non-secretory ribonuclease
Gene
Rnase2, Ear4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331Substrate By similarity
Active sitei38 – 381Proton acceptor By similarity
Active sitei150 – 1501Proton donor By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. pancreatic ribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-secretory ribonuclease (EC:3.1.27.5)
    Alternative name(s):
    Eosinophil cationic-type ribonuclease 4
    MR-4
    Ribonuclease 2
    Short name:
    RNase 2
    Gene namesi
    Name:Rnase2
    Synonyms:Ear4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1858238. Ear4.

    Subcellular locationi

    Lysosome Inferred. Cytoplasmic granule
    Note: Matrix of eosinophil's large specific granule.

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525 Reviewed prediction
    Add
    BLAST
    Chaini26 – 155130Non-secretory ribonuclease
    PRO_0000030882Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi41 – 411N-linked (GlcNAc...) Reviewed prediction
    Disulfide bondi47 ↔ 106 By similarity
    Modified residuei57 – 571Nitrated tyrosine By similarity
    Disulfide bondi61 ↔ 118 By similarity
    Disulfide bondi79 ↔ 133 By similarity
    Glycosylationi83 – 831N-linked (GlcNAc...) Reviewed prediction
    Disulfide bondi86 ↔ 94 By similarity
    Glycosylationi88 – 881N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi107 – 1071N-linked (GlcNAc...) Reviewed prediction

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Nitration

    Proteomic databases

    PRIDEiO35291.

    PTM databases

    PhosphoSiteiO35291.

    Expressioni

    Gene expression databases

    GenevestigatoriO35291.

    Interactioni

    Subunit structurei

    Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur By similarity.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000056293.

    Structurei

    3D structure databases

    ProteinModelPortaliO35291.
    SMRiO35291. Positions 31-152.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni62 – 665Substrate binding By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG39501.
    HOGENOMiHOG000276882.
    HOVERGENiHBG008396.
    InParanoidiO35291.
    PhylomeDBiO35291.

    Family and domain databases

    Gene3Di3.10.130.10. 1 hit.
    InterProiIPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view]
    PANTHERiPTHR11437. PTHR11437. 1 hit.
    PfamiPF00074. RnaseA. 1 hit.
    [Graphical view]
    PRINTSiPR00794. RIBONUCLEASE.
    ProDomiPD000535. RNaseA. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00092. RNAse_Pc. 1 hit.
    [Graphical view]
    SUPFAMiSSF54076. SSF54076. 1 hit.
    PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35291-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGPKLLESRL CLLLLLGLVL MLASCQAQIL SQKFYTQHIY NSTYPRCDAV    50
    MRVVNRYRPR CKDINTFLHT SFADVVAVCG HPNITCNNLT RKNCHASSFQ 100
    VFITFCNLTM PTRICTQCRY QTTGSVKYYR VACENRTPQD TPMYPVVPVH 150
    LDGTF 155
    Length:155
    Mass (Da):17,723
    Last modified:January 1, 1998 - v1
    Checksum:i36879AAF1CCFB961
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017259 Genomic DNA. Translation: AAC53490.1.
    RefSeqiNP_001017422.1. NM_001017422.1.
    UniGeneiMm.377124.

    Genome annotation databases

    GeneIDi53877.
    KEGGimmu:53877.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017259 Genomic DNA. Translation: AAC53490.1 .
    RefSeqi NP_001017422.1. NM_001017422.1.
    UniGenei Mm.377124.

    3D structure databases

    ProteinModelPortali O35291.
    SMRi O35291. Positions 31-152.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000056293.

    PTM databases

    PhosphoSitei O35291.

    Proteomic databases

    PRIDEi O35291.

    Protocols and materials databases

    DNASUi 53877.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 53877.
    KEGGi mmu:53877.

    Organism-specific databases

    CTDi 53877.
    MGIi MGI:1858238. Ear4.

    Phylogenomic databases

    eggNOGi NOG39501.
    HOGENOMi HOG000276882.
    HOVERGENi HBG008396.
    InParanoidi O35291.
    PhylomeDBi O35291.

    Miscellaneous databases

    NextBioi 310745.
    PROi O35291.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori O35291.

    Family and domain databases

    Gene3Di 3.10.130.10. 1 hit.
    InterProi IPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view ]
    PANTHERi PTHR11437. PTHR11437. 1 hit.
    Pfami PF00074. RnaseA. 1 hit.
    [Graphical view ]
    PRINTSi PR00794. RIBONUCLEASE.
    ProDomi PD000535. RNaseA. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00092. RNAse_Pc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54076. SSF54076. 1 hit.
    PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of four novel murine ribonuclease genes: unusual expansion within the ribonuclease A gene family."
      Batten D., Dyer K.D., Domachowske J.B., Rosenberg H.F.
      Nucleic Acids Res. 25:4235-4239(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Fibroblast.
    2. Cited for: NITRATION.

    Entry informationi

    Entry nameiRNAS2_MOUSE
    AccessioniPrimary (citable) accession number: O35291
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: April 16, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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