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O35291

- RNAS2_MOUSE

UniProt

O35291 - RNAS2_MOUSE

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Protein

Non-secretory ribonuclease

Gene

Rnase2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331SubstrateBy similarity
Active sitei38 – 381Proton acceptorBy similarity
Active sitei150 – 1501Proton donorBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. pancreatic ribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Non-secretory ribonuclease (EC:3.1.27.5)
Alternative name(s):
Eosinophil cationic-type ribonuclease 4
MR-4
Ribonuclease 2
Short name:
RNase 2
Gene namesi
Name:Rnase2
Synonyms:Ear4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1858238. Ear4.

Subcellular locationi

Lysosome Curated. Cytoplasmic granule
Note: Matrix of eosinophil's large specific granule.

GO - Cellular componenti

  1. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 155130Non-secretory ribonucleasePRO_0000030882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi47 ↔ 106By similarity
Modified residuei57 – 571Nitrated tyrosineBy similarity
Disulfide bondi61 ↔ 118By similarity
Disulfide bondi79 ↔ 133By similarity
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi86 ↔ 94By similarity
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Nitration

Proteomic databases

PRIDEiO35291.

PTM databases

PhosphoSiteiO35291.

Expressioni

Gene expression databases

GenevestigatoriO35291.

Interactioni

Subunit structurei

Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056293.

Structurei

3D structure databases

ProteinModelPortaliO35291.
SMRiO35291. Positions 31-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 665Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG39501.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiO35291.
PhylomeDBiO35291.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35291-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGPKLLESRL CLLLLLGLVL MLASCQAQIL SQKFYTQHIY NSTYPRCDAV
60 70 80 90 100
MRVVNRYRPR CKDINTFLHT SFADVVAVCG HPNITCNNLT RKNCHASSFQ
110 120 130 140 150
VFITFCNLTM PTRICTQCRY QTTGSVKYYR VACENRTPQD TPMYPVVPVH

LDGTF
Length:155
Mass (Da):17,723
Last modified:January 1, 1998 - v1
Checksum:i36879AAF1CCFB961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017259 Genomic DNA. Translation: AAC53490.1.
RefSeqiNP_001017422.1. NM_001017422.1.
UniGeneiMm.377124.

Genome annotation databases

GeneIDi53877.
KEGGimmu:53877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017259 Genomic DNA. Translation: AAC53490.1 .
RefSeqi NP_001017422.1. NM_001017422.1.
UniGenei Mm.377124.

3D structure databases

ProteinModelPortali O35291.
SMRi O35291. Positions 31-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000056293.

PTM databases

PhosphoSitei O35291.

Proteomic databases

PRIDEi O35291.

Protocols and materials databases

DNASUi 53877.
Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 53877.
KEGGi mmu:53877.

Organism-specific databases

CTDi 53877.
MGIi MGI:1858238. Ear4.

Phylogenomic databases

eggNOGi NOG39501.
HOGENOMi HOG000276882.
HOVERGENi HBG008396.
InParanoidi O35291.
PhylomeDBi O35291.

Miscellaneous databases

NextBioi 310745.
PROi O35291.
SOURCEi Search...

Gene expression databases

Genevestigatori O35291.

Family and domain databases

Gene3Di 3.10.130.10. 1 hit.
InterProi IPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view ]
PANTHERi PTHR11437. PTHR11437. 1 hit.
Pfami PF00074. RnaseA. 1 hit.
[Graphical view ]
PRINTSi PR00794. RIBONUCLEASE.
ProDomi PD000535. RNaseA. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00092. RNAse_Pc. 1 hit.
[Graphical view ]
SUPFAMi SSF54076. SSF54076. 1 hit.
PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of four novel murine ribonuclease genes: unusual expansion within the ribonuclease A gene family."
    Batten D., Dyer K.D., Domachowske J.B., Rosenberg H.F.
    Nucleic Acids Res. 25:4235-4239(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Fibroblast.
  2. Cited for: NITRATION.

Entry informationi

Entry nameiRNAS2_MOUSE
AccessioniPrimary (citable) accession number: O35291
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3