O35290 (ECP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eosinophil cationic-type ribonuclease 3

Alternative name(s):
MR-3

Gene names

Name:	Ear3
Synonyms:	Rnase3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities

Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 156	131	Eosinophil cationic-type ribonuclease 3		PRO_0000030869
Regions
	Region	62 – 66	5	Substrate binding By similarity
Sites
	Active site	38	1	Proton acceptor By similarity
	Active site	151	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 106		By similarity
	Disulfide bond	61 ↔ 119		By similarity
	Disulfide bond	79 ↔ 134		By similarity
	Disulfide bond	86 ↔ 94		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35290 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C9C2B6ECEB5DA06C	FASTA	156	17,745
	10 20 30 40 50 60 MGPKLLESRL CLLLLLRLVL MLASCLGQTP SRWFAIQHIN NNTNLRCNVE MLRINRFRRT 70 80 90 100 110 120 CKGLNTFLHT SFANAVGVCG NPSGLCSDNI SRNCHNSSSR VHITVCNITS RRRIPYTQCR 130 140 150 YQPRRSVEYY TVACNPRTSL DSPMYPVVPV HLDGTF

References

[1]	"Molecular cloning of four novel murine ribonuclease genes: unusual expansion within the ribonuclease A gene family." Batten D., Dyer K.D., Domachowske J.B., Rosenberg H.F. Nucleic Acids Res. 25:4235-4239(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017258 Genomic DNA. Translation: AAC53489.1.
IPI	IPI00128862.
RefSeq	NP_059084.1. NM_017388.1.
UniGene	Mm.327386.
3D structure databases
ProteinModelPortal	O35290.
SMR	O35290. Positions 29-155.
ModBase	Search...
Proteomic databases
PaxDb	O35290.
PRIDE	O35290.
Protocols and materials databases
DNASU	53876.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	53876.
KEGG	mmu:53876.
Organism-specific databases
CTD	53876.
MGI	MGI:1858237. Ear3.
Phylogenomic databases
eggNOG	NOG39501.
HOVERGEN	HBG008396.
InParanoid	O35290.
Gene expression databases
Genevestigator	O35290.
GermOnline	ENSMUSG00000072596. Mus musculus.
Family and domain databases
Gene3D	3.10.130.10. 1 hit.
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
PANTHER	PTHR11437. PTHR11437. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NR2F1. mouse.
NextBio	310741.
SOURCE	Search...

Entry information

Entry name

ECP3_MOUSE

Accession

Primary (citable) accession number: O35290

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program