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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Gene

Dhx15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 1678ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15By similarity (EC:3.6.4.13By similarity)
Alternative name(s):
DEAH box protein 15
Gene namesi
Name:Dhx15
Synonyms:Ddx15, Deah9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1099786. Dhx15.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 795795Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15PRO_0000055140Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei488 – 4881N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO35286.
PaxDbiO35286.
PRIDEiO35286.

PTM databases

iPTMnetiO35286.
PhosphoSiteiO35286.
SwissPalmiO35286.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiO35286.
CleanExiMM_DHX15.
ExpressionAtlasiO35286. baseline and differential.
GenevisibleiO35286. MM.

Interactioni

Subunit structurei

Interacts with SSB/La. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Identified in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient. Interacts with TFIP11; indicative for a recruitment to the IL complex. Interacts with GPATCH2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tfip11Q9ERA63EBI-8322087,EBI-8338752

Protein-protein interaction databases

BioGridi199083. 2 interactions.
IntActiO35286. 5 interactions.
MINTiMINT-4093075.
STRINGi10090.ENSMUSP00000031061.

Structurei

3D structure databases

ProteinModelPortaliO35286.
SMRiO35286. Positions 115-787.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 313167Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini338 – 518181Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi260 – 2634DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi346 – 3494Poly-Glu
Compositional biasi395 – 3984Poly-Gln
Compositional biasi403 – 4064Poly-Pro

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiO35286.
KOiK12820.
OMAiFKNEMQD.
OrthoDBiEOG7FXZXR.
PhylomeDBiO35286.
TreeFamiTF105735.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRHRLDLG EDYPSGKKRA GTDGKDRERD RDREDRSKDR DRERDRGDRE
60 70 80 90 100
REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS
110 120 130 140 150
THTGHTGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL
160 170 180 190 200
VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS
210 220 230 240 250
VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL
260 270 280 290 300
LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF
310 320 330 340 350
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG
360 370 380 390 400
DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI
410 420 430 440 450
FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR
460 470 480 490 500
IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP
510 520 530 540 550
EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN
560 570 580 590 600
DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF
610 620 630 640 650
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN
660 670 680 690 700
YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ
710 720 730 740 750
VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC
760 770 780 790
TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY
Length:795
Mass (Da):91,007
Last modified:June 6, 2003 - v2
Checksum:i25A97254318E497A
GO

Sequence cautioni

The sequence BC003745 differs from that shown. Reason: Frameshift at position 409. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti388 – 3892LY → FS (PubMed:9342318).Curated
Sequence conflicti391 – 3933TLP → YTS (PubMed:9342318).Curated
Sequence conflicti757 – 7582WL → CW (PubMed:9342318).Curated
Sequence conflicti759 – 79537Missing (PubMed:9342318).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017153 mRNA. Translation: AAC36129.1.
BC003745 mRNA. No translation available.
CCDSiCCDS39085.1.
RefSeqiNP_031865.2. NM_007839.3.
UniGeneiMm.993.

Genome annotation databases

EnsembliENSMUST00000031061; ENSMUSP00000031061; ENSMUSG00000029169.
GeneIDi13204.
KEGGimmu:13204.
UCSCiuc008xkf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017153 mRNA. Translation: AAC36129.1.
BC003745 mRNA. No translation available.
CCDSiCCDS39085.1.
RefSeqiNP_031865.2. NM_007839.3.
UniGeneiMm.993.

3D structure databases

ProteinModelPortaliO35286.
SMRiO35286. Positions 115-787.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199083. 2 interactions.
IntActiO35286. 5 interactions.
MINTiMINT-4093075.
STRINGi10090.ENSMUSP00000031061.

PTM databases

iPTMnetiO35286.
PhosphoSiteiO35286.
SwissPalmiO35286.

Proteomic databases

EPDiO35286.
PaxDbiO35286.
PRIDEiO35286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031061; ENSMUSP00000031061; ENSMUSG00000029169.
GeneIDi13204.
KEGGimmu:13204.
UCSCiuc008xkf.2. mouse.

Organism-specific databases

CTDi1665.
MGIiMGI:1099786. Dhx15.

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiO35286.
KOiK12820.
OMAiFKNEMQD.
OrthoDBiEOG7FXZXR.
PhylomeDBiO35286.
TreeFamiTF105735.

Miscellaneous databases

NextBioi283358.
PROiO35286.
SOURCEiSearch...

Gene expression databases

BgeeiO35286.
CleanExiMM_DHX15.
ExpressionAtlasiO35286. baseline and differential.
GenevisibleiO35286. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43."
    Gee S., Krauss S.W., Miller E., Aoyagi K., Arenas J., Conboy J.G.
    Proc. Natl. Acad. Sci. U.S.A. 94:11803-11807(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiDHX15_MOUSE
AccessioniPrimary (citable) accession number: O35286
Secondary accession number(s): Q99L91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 6, 2003
Last modified: May 11, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.