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O35284

- BATF_MOUSE

UniProt

O35284 - BATF_MOUSE

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Protein

Basic leucine zipper transcriptional factor ATF-like

Gene

Batf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8+ dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8+ dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8+ T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs.11 Publications

GO - Molecular functioni

  1. sequence-specific DNA binding Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: UniProtKB
  2. cytokine production Source: UniProtKB
  3. defense response to protozoan Source: UniProtKB
  4. DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  5. hematopoietic stem cell differentiation Source: UniProtKB
  6. isotype switching Source: UniProtKB
  7. lymphoid progenitor cell differentiation Source: UniProtKB
  8. myeloid dendritic cell differentiation Source: UniProtKB
  9. T-helper 17 cell differentiation Source: UniProtKB
  10. T-helper 17 cell lineage commitment Source: UniProtKB
  11. T-helper 2 cell differentiation Source: UniProtKB
  12. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Basic leucine zipper transcriptional factor ATF-like
Alternative name(s):
B-cell-activating transcription factor
Short name:
B-ATF
Gene namesi
Name:Batf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1859147. Batf.

Subcellular locationi

Nucleus. Cytoplasm
Note: Present in the nucleus and cytoplasm, but shows increased nuclear translocation after activation of T-cells.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are fertile and healthy. They display a normal thymus, spleen and lymph node development, and normal CD4+ and CD8+ T-cell development. They also show normal development of natural killer T-cells, B-cells, and conventional and plasmacytoid dendritic cells. They however show defects in T-helper 17 cells (Th17) differentiation and are resistant to experimental autoimmune encephalomyelitis. Loss of follicular T-helper cells (TfH), as well as less production of antibodies with switched isotypes in B-cells is also observed.4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi43 – 431S → A: Decreased phosphorylation; when associated with A-48. 1 Publication
Mutagenesisi43 – 431S → D: Retains the ability to dimerize with JUNB and localization in the nucleus but abolishes DNA-binding. 1 Publication
Mutagenesisi48 – 481T → A: Decreased phosphorylation; when associated with A-43. 1 Publication
Mutagenesisi55 – 551H → Q: Impairs interaction with IRF4 and the recruitment of IRF4 to AICE motifs, leading to defects in mediate differentiation of Th17 cells. Loss of function; when associated with A-56; D-63 and K-77. 2 Publications
Mutagenesisi56 – 561L → A: Loss of function; when associated with Q-55; D-63 and K-77. 1 Publication
Mutagenesisi63 – 631K → D: Retains 50% of activity; when associated with 69-Q-T-70 and K-77. Loss of function; when associated with Q-55; A-56 and K-77. 1 Publication
Mutagenesisi69 – 702RK → QT: Retains 50% of activity; when associated with D-63 and K-77. 1 Publication
Mutagenesisi77 – 771E → K: Does not affect interaction with IRF4 and ability to mediate differentiation of Th17 cells. Retains 50% of activity; when associated with D63 and 69-Q-T-70. Loss of function; when associated with Q-55; A-56 and D-63. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 125125Basic leucine zipper transcriptional factor ATF-likePRO_0000076596Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431Phosphoserine1 Publication
Modified residuei48 – 481Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO35284.

PTM databases

PhosphoSiteiO35284.

Expressioni

Tissue specificityi

Detected in postnatal and adult lymphoid tissues such as thymus, spleen and lymph nodes. In thymus most concentrated expression is found in the immediate cortical layer. Differentially expressed during T-cell development in thymus. Highly expressed in Th17, Th1 and Th2 cells and in activated B-cells.3 Publications

Inductioni

Up-regulated by STAT3 in response to DNA damage. Induces by IL12 at late effector stage. Down-regulated by STAT5 in follicular T-helper cells (TfH).4 Publications

Gene expression databases

BgeeiO35284.
GenevestigatoriO35284.

Interactioni

Subunit structurei

Heterodimer; mainly heterodimerizes with JUNB. The BATF-JUNB heterodimer interacts with IRF4 and IRF8. Interacts (via bZIP domain) with IRF4 and IRF8; the interaction is direct. Also forms heterodimers with JUN and JUND. Also Interacts with IFI35.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Irf4Q642877EBI-6398523,EBI-6398485

Protein-protein interaction databases

BioGridi207278. 1 interaction.
IntActiO35284. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO35284.
SMRiO35284. Positions 24-84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 8964bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni28 – 5023Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni54 – 7522Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the bZIP family.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257556.
GeneTreeiENSGT00390000016869.
HOGENOMiHOG000236325.
HOVERGENiHBG039173.
InParanoidiO35284.
KOiK09034.
OMAiEEMKHFT.
OrthoDBiEOG7H794S.
PhylomeDBiO35284.
TreeFamiTF332340.

Family and domain databases

InterProiIPR000837. AP-1.
IPR004827. bZIP.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
PRINTSiPR00042. LEUZIPPRFOS.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35284-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPHSSDSSDS SFSRSPPPGK QDSSDDVRKV QRREKNRIAA QKSRQRQTQK
60 70 80 90 100
ADTLHLESED LEKQNAALRK EIKQLTEELK YFTSVLSSHE PLCSVLASGT
110 120
PSPPEVVYSA HAFHQPHISS PRFQP
Length:125
Mass (Da):14,065
Last modified:January 1, 1998 - v1
Checksum:iA23B00D6E0827938
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF017021 mRNA. Translation: AAB70251.1.
AK018587 mRNA. Translation: BAB31294.1.
BC132410 mRNA. Translation: AAI32411.1.
BC132412 mRNA. Translation: AAI32413.1.
CCDSiCCDS26061.1.
RefSeqiNP_058047.1. NM_016767.2.
UniGeneiMm.6672.

Genome annotation databases

EnsembliENSMUST00000040536; ENSMUSP00000040706; ENSMUSG00000034266.
GeneIDi53314.
KEGGimmu:53314.
UCSCiuc007ohd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF017021 mRNA. Translation: AAB70251.1 .
AK018587 mRNA. Translation: BAB31294.1 .
BC132410 mRNA. Translation: AAI32411.1 .
BC132412 mRNA. Translation: AAI32413.1 .
CCDSi CCDS26061.1.
RefSeqi NP_058047.1. NM_016767.2.
UniGenei Mm.6672.

3D structure databases

ProteinModelPortali O35284.
SMRi O35284. Positions 24-84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207278. 1 interaction.
IntActi O35284. 1 interaction.

PTM databases

PhosphoSitei O35284.

Proteomic databases

PRIDEi O35284.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040536 ; ENSMUSP00000040706 ; ENSMUSG00000034266 .
GeneIDi 53314.
KEGGi mmu:53314.
UCSCi uc007ohd.1. mouse.

Organism-specific databases

CTDi 10538.
MGIi MGI:1859147. Batf.

Phylogenomic databases

eggNOGi NOG257556.
GeneTreei ENSGT00390000016869.
HOGENOMi HOG000236325.
HOVERGENi HBG039173.
InParanoidi O35284.
KOi K09034.
OMAi EEMKHFT.
OrthoDBi EOG7H794S.
PhylomeDBi O35284.
TreeFami TF332340.

Miscellaneous databases

NextBioi 310101.
PROi O35284.
SOURCEi Search...

Gene expression databases

Bgeei O35284.
Genevestigatori O35284.

Family and domain databases

InterProi IPR000837. AP-1.
IPR004827. bZIP.
[Graphical view ]
Pfami PF00170. bZIP_1. 1 hit.
[Graphical view ]
PRINTSi PR00042. LEUZIPPRFOS.
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
PROSITEi PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of murine BATF: a negative regulator of activator protein-1 activity in the thymus."
    Williams K.L., Nanda I., Lyons G.E., Kuo C.T., Schmid M., Leiden J.M., Kaplan M.H., Taparowsky E.J.
    Eur. J. Immunol. 31:1620-1627(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cecum.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. "Stat3-dependent induction of BATF in M1 mouse myeloid leukemia cells."
    Senga T., Iwamoto T., Humphrey S.E., Yokota T., Taparowsky E.J., Hamaguchi M.
    Oncogene 21:8186-8191(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY STAT3.
  5. "Phosphorylation of BATF regulates DNA binding: a novel mechanism for AP-1 (activator protein-1) regulation."
    Deppmann C.D., Thornton T.M., Utama F.E., Taparowsky E.J.
    Biochem. J. 374:423-431(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DNA-BINDING, INTERACTION WITH JUNB, PHOSPHORYLATION AT SER-43 AND THR-48, MUTAGENESIS OF SER-43 AND THR-48.
  6. "BATF transgenic mice reveal a role for activator protein-1 in NKT cell development."
    Williams K.L., Zullo A.J., Kaplan M.H., Brutkiewicz R.R., Deppmann C.D., Vinson C., Taparowsky E.J.
    J. Immunol. 170:2417-2426(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH JUNB.
  8. "Batf coordinates multiple aspects of B and T cell function required for normal antibody responses."
    Betz B.C., Jordan-Williams K.L., Wang C., Kang S.G., Liao J., Logan M.R., Kim C.H., Taparowsky E.J.
    J. Exp. Med. 207:933-942(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  9. "The transcription factor BATF controls the global regulators of class-switch recombination in both B cells and T cells."
    Ise W., Kohyama M., Schraml B.U., Zhang T., Schwer B., Basu U., Alt F.W., Tang J., Oltz E.M., Murphy T.L., Murphy K.M.
    Nat. Immunol. 12:536-543(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, DISRUPTION PHENOTYPE.
  10. "Transcription factor c-Maf mediates the TGF-beta-dependent suppression of IL-22 production in T(H)17 cells."
    Rutz S., Noubade R., Eidenschenk C., Ota N., Zeng W., Zheng Y., Hackney J., Ding J., Singh H., Ouyang W.
    Nat. Immunol. 12:1238-1245(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Basic leucine zipper transcription factor, ATF-like (BATF) regulates epigenetically and energetically effector CD8 T-cell differentiation via Sirt1 expression."
    Kuroda S., Yamazaki M., Abe M., Sakimura K., Takayanagi H., Iwai Y.
    Proc. Natl. Acad. Sci. U.S.A. 108:14885-14889(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, INDUCTION.
  12. Cited for: FUNCTION, INDUCTION BY STAT3.
  13. Cited for: FUNCTION.
  14. "STAT5 protein negatively regulates T follicular helper (Tfh) cell generation and function."
    Nurieva R.I., Podd A., Chen Y., Alekseev A.M., Yu M., Qi X., Huang H., Wen R., Wang J., Li H.S., Watowich S.S., Qi H., Dong C., Wang D.
    J. Biol. Chem. 287:11234-11239(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY STAT5.
  15. Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH IRF4; IRF8 AND JUNB, MUTAGENESIS OF HIS-55; LEU-56; LYS-63; 69-ARG-LYS-70 AND GLU-77.
  16. "BATF-JUN is critical for IRF4-mediated transcription in T cells."
    Li P., Spolski R., Liao W., Wang L., Murphy T.L., Murphy K.M., Leonard W.J.
    Nature 490:543-546(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH IRF4 AND JUNB.
  17. Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH IRF4 AND JUNB, MUTAGENESIS OF HIS-55 AND GLU-77.

Entry informationi

Entry nameiBATF_MOUSE
AccessioniPrimary (citable) accession number: O35284
Secondary accession number(s): A2RT86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3