Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Platelet-activating factor acetylhydrolase IB subunit beta

Gene

Pafah1b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.

Catalytic activityi

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48By similarity1
Active sitei193By similarity1
Active sitei196By similarity1

GO - Molecular functioni

  • 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
  • platelet-activating factor acetyltransferase activity Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.47. 5301.
ReactomeiR-RNO-6798695. Neutrophil degranulation.
R-RNO-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase IB subunit beta (EC:3.1.1.47)
Alternative name(s):
PAF acetylhydrolase 30 kDa subunit
Short name:
PAF-AH 30 kDa subunit
PAF-AH subunit beta
Short name:
PAFAH subunit beta
Platelet-activating factor acetylhydrolase alpha 2 subunit
Short name:
PAF-AH alpha 2
Gene namesi
Name:Pafah1b2
Synonyms:Pafahb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi620332. Pafah1b2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000581532 – 229Platelet-activating factor acetylhydrolase IB subunit betaAdd BLAST228

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Modified residuei64PhosphoserineCombined sources1
Modified residuei220PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO35264.
PRIDEiO35264.

PTM databases

iPTMnetiO35264.
PhosphoSitePlusiO35264.

Expressioni

Tissue specificityi

Expressed in heart, brain, spleen, lung, liver, kidney and testis. Not expressed in skeletal muscle.

Gene expression databases

BgeeiENSRNOG00000057102.
GenevisibleiO35264. RN.

Interactioni

Subunit structurei

Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Binary interactionsi

WithEntry#Exp.IntActNotes
PAFAH1B1P430332EBI-915500,EBI-1007886From Bos taurus.

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

IntActiO35264. 2 interactors.
STRINGi10116.ENSRNOP00000024828.

Structurei

3D structure databases

ProteinModelPortaliO35264.
SMRiO35264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IMK6. Eukaryota.
ENOG410XPWQ. LUCA.
GeneTreeiENSGT00390000016520.
HOGENOMiHOG000232143.
HOVERGENiHBG053477.
InParanoidiO35264.
KOiK16795.
OMAiRGQQPNK.
OrthoDBiEOG091G0OII.
PhylomeDBiO35264.
TreeFamiTF323955.

Family and domain databases

InterProiView protein in InterPro
IPR013830. SGNH_hydro.
PfamiView protein in Pfam
PF13472. Lipase_GDSL_2. 1 hit.
SUPFAMiSSF52266. SSF52266. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQGDSNPAA IPHAAEDIQG DDRWMSQHNR FVLDCKDKEP DVLFVGDSMV
60 70 80 90 100
QLMQQYEIWR ELFSPLHALN FGIGGDTTRH VLWRLKNGEL ENIKPKVIVV
110 120 130 140 150
WVGTNNHENT AEEVAGGIEA IVQLINTRQP QAKIIVLGLL PRGEKPNPLR
160 170 180 190 200
QKNAKVNQLL KVSLPKLANV QLLDIDGGFV HSDGAISCHD MFDFLHLTGG
210 220
GYAKICKPLH ELIMQLLEET PEEKQTTIA
Length:229
Mass (Da):25,581
Last modified:January 1, 1998 - v1
Checksum:iB4D24048621AB182
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016048 mRNA. Translation: AAC27974.1.
BC081714 mRNA. Translation: AAH81714.1.
RefSeqiNP_071782.1. NM_022387.3.
XP_006243020.1. XM_006242958.3.
UniGeneiRn.24751.

Genome annotation databases

EnsembliENSRNOT00000082508; ENSRNOP00000068672; ENSRNOG00000057102.
GeneIDi64189.
KEGGirno:64189.
UCSCiRGD:620332. rat.

Similar proteinsi

Entry informationi

Entry nameiPA1B2_RAT
AccessioniPrimary (citable) accession number: O35264
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: August 30, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families