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O35264

- PA1B2_RAT

UniProt

O35264 - PA1B2_RAT

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Protein

Platelet-activating factor acetylhydrolase IB subunit beta

Gene

Pafah1b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.

Catalytic activityi

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei48 – 481By similarity
Active sitei193 – 1931By similarity
Active sitei196 – 1961By similarity

GO - Molecular functioni

  1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
  2. platelet-activating factor acetyltransferase activity Source: RGD
  3. protein heterodimerization activity Source: RGD
  4. protein homodimerization activity Source: RGD

GO - Biological processi

  1. brain development Source: RGD
  2. lipid catabolic process Source: UniProtKB-KW
  3. positive regulation of macroautophagy Source: Ensembl
  4. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase IB subunit beta (EC:3.1.1.47)
Alternative name(s):
PAF acetylhydrolase 30 kDa subunit
Short name:
PAF-AH 30 kDa subunit
PAF-AH subunit beta
Short name:
PAFAH subunit beta
Platelet-activating factor acetylhydrolase alpha 2 subunit
Short name:
PAF-AH alpha 2
Gene namesi
Name:Pafah1b2
Synonyms:Pafahb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi620332. Pafah1b2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: RGD
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleolus Source: Ensembl
  4. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 229228Platelet-activating factor acetylhydrolase IB subunit betaPRO_0000058153Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO35264.
PRIDEiO35264.

PTM databases

PhosphoSiteiO35264.

Expressioni

Tissue specificityi

Expressed in heart, brain, spleen, lung, liver, kidney and testis. Not expressed in skeletal muscle.

Gene expression databases

GenevestigatoriO35264.

Interactioni

Subunit structurei

Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Binary interactionsi

WithEntry#Exp.IntActNotes
PAFAH1B1P430332EBI-915500,EBI-1007886From a different organism.

Protein-protein interaction databases

IntActiO35264. 2 interactions.
STRINGi10116.ENSRNOP00000024828.

Structurei

3D structure databases

ProteinModelPortaliO35264.
SMRiO35264. Positions 6-217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG69837.
GeneTreeiENSGT00390000016520.
HOGENOMiHOG000232143.
HOVERGENiHBG053477.
InParanoidiO35264.
KOiK16795.
OMAiNIRPKVV.
OrthoDBiEOG7HB5BS.
PhylomeDBiO35264.
TreeFamiTF323955.

Family and domain databases

Gene3Di3.40.50.1110. 1 hit.
InterProiIPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35264-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQGDSNPAA IPHAAEDIQG DDRWMSQHNR FVLDCKDKEP DVLFVGDSMV
60 70 80 90 100
QLMQQYEIWR ELFSPLHALN FGIGGDTTRH VLWRLKNGEL ENIKPKVIVV
110 120 130 140 150
WVGTNNHENT AEEVAGGIEA IVQLINTRQP QAKIIVLGLL PRGEKPNPLR
160 170 180 190 200
QKNAKVNQLL KVSLPKLANV QLLDIDGGFV HSDGAISCHD MFDFLHLTGG
210 220
GYAKICKPLH ELIMQLLEET PEEKQTTIA
Length:229
Mass (Da):25,581
Last modified:January 1, 1998 - v1
Checksum:iB4D24048621AB182
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016048 mRNA. Translation: AAC27974.1.
BC081714 mRNA. Translation: AAH81714.1.
RefSeqiNP_071782.1. NM_022387.3.
XP_006243019.1. XM_006242957.2.
XP_006243020.1. XM_006242958.2.
XP_008764394.1. XM_008766172.1.
UniGeneiRn.24751.

Genome annotation databases

EnsembliENSRNOT00000024828; ENSRNOP00000024828; ENSRNOG00000018032.
GeneIDi64189.
KEGGirno:64189.
UCSCiRGD:620332. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016048 mRNA. Translation: AAC27974.1 .
BC081714 mRNA. Translation: AAH81714.1 .
RefSeqi NP_071782.1. NM_022387.3.
XP_006243019.1. XM_006242957.2.
XP_006243020.1. XM_006242958.2.
XP_008764394.1. XM_008766172.1.
UniGenei Rn.24751.

3D structure databases

ProteinModelPortali O35264.
SMRi O35264. Positions 6-217.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O35264. 2 interactions.
STRINGi 10116.ENSRNOP00000024828.

PTM databases

PhosphoSitei O35264.

Proteomic databases

PaxDbi O35264.
PRIDEi O35264.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000024828 ; ENSRNOP00000024828 ; ENSRNOG00000018032 .
GeneIDi 64189.
KEGGi rno:64189.
UCSCi RGD:620332. rat.

Organism-specific databases

CTDi 5049.
RGDi 620332. Pafah1b2.

Phylogenomic databases

eggNOGi NOG69837.
GeneTreei ENSGT00390000016520.
HOGENOMi HOG000232143.
HOVERGENi HBG053477.
InParanoidi O35264.
KOi K16795.
OMAi NIRPKVV.
OrthoDBi EOG7HB5BS.
PhylomeDBi O35264.
TreeFami TF323955.

Miscellaneous databases

NextBioi 612844.
PROi O35264.

Gene expression databases

Genevestigatori O35264.

Family and domain databases

Gene3Di 3.40.50.1110. 1 hit.
InterProi IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of cDNAs encoding alpha1, alpha2, and beta subunits of rat brain platelet-activating factor acetylhydrolase."
    Watanabe M., Aoki J., Manya H., Arai H., Inoue K.
    Biochim. Biophys. Acta 1401:73-79(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Lubec G., Chen W.-Q., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 61-79 AND 134-142, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry nameiPA1B2_RAT
AccessioniPrimary (citable) accession number: O35264
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3