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O35264

- PA1B2_RAT

UniProt

O35264 - PA1B2_RAT

Protein

Platelet-activating factor acetylhydrolase IB subunit beta

Gene

Pafah1b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.

    Catalytic activityi

    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei48 – 481By similarity
    Active sitei193 – 1931By similarity
    Active sitei196 – 1961By similarity

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
    2. platelet-activating factor acetyltransferase activity Source: RGD
    3. protein binding Source: IntAct
    4. protein heterodimerization activity Source: RGD
    5. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. brain development Source: RGD
    2. lipid catabolic process Source: UniProtKB-KW
    3. positive regulation of macroautophagy Source: Ensembl
    4. spermatogenesis Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase IB subunit beta (EC:3.1.1.47)
    Alternative name(s):
    PAF acetylhydrolase 30 kDa subunit
    Short name:
    PAF-AH 30 kDa subunit
    PAF-AH subunit beta
    Short name:
    PAFAH subunit beta
    Platelet-activating factor acetylhydrolase alpha 2 subunit
    Short name:
    PAF-AH alpha 2
    Gene namesi
    Name:Pafah1b2
    Synonyms:Pafahb
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi620332. Pafah1b2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. nucleolus Source: Ensembl
    3. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 229228Platelet-activating factor acetylhydrolase IB subunit betaPRO_0000058153Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiO35264.
    PRIDEiO35264.

    PTM databases

    PhosphoSiteiO35264.

    Expressioni

    Tissue specificityi

    Expressed in heart, brain, spleen, lung, liver, kidney and testis. Not expressed in skeletal muscle.

    Gene expression databases

    GenevestigatoriO35264.

    Interactioni

    Subunit structurei

    Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PAFAH1B1P430332EBI-915500,EBI-1007886From a different organism.

    Protein-protein interaction databases

    IntActiO35264. 2 interactions.
    STRINGi10116.ENSRNOP00000024828.

    Structurei

    3D structure databases

    ProteinModelPortaliO35264.
    SMRiO35264. Positions 6-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG69837.
    GeneTreeiENSGT00390000016520.
    HOGENOMiHOG000232143.
    HOVERGENiHBG053477.
    InParanoidiO35264.
    KOiK16795.
    OMAiNIRPKVV.
    OrthoDBiEOG7HB5BS.
    PhylomeDBiO35264.
    TreeFamiTF323955.

    Family and domain databases

    Gene3Di3.40.50.1110. 1 hit.
    InterProiIPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35264-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQGDSNPAA IPHAAEDIQG DDRWMSQHNR FVLDCKDKEP DVLFVGDSMV    50
    QLMQQYEIWR ELFSPLHALN FGIGGDTTRH VLWRLKNGEL ENIKPKVIVV 100
    WVGTNNHENT AEEVAGGIEA IVQLINTRQP QAKIIVLGLL PRGEKPNPLR 150
    QKNAKVNQLL KVSLPKLANV QLLDIDGGFV HSDGAISCHD MFDFLHLTGG 200
    GYAKICKPLH ELIMQLLEET PEEKQTTIA 229
    Length:229
    Mass (Da):25,581
    Last modified:January 1, 1998 - v1
    Checksum:iB4D24048621AB182
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF016048 mRNA. Translation: AAC27974.1.
    BC081714 mRNA. Translation: AAH81714.1.
    RefSeqiNP_071782.1. NM_022387.3.
    XP_006243019.1. XM_006242957.1.
    XP_006243020.1. XM_006242958.1.
    UniGeneiRn.24751.

    Genome annotation databases

    EnsembliENSRNOT00000024828; ENSRNOP00000024828; ENSRNOG00000018032.
    GeneIDi64189.
    KEGGirno:64189.
    UCSCiRGD:620332. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF016048 mRNA. Translation: AAC27974.1 .
    BC081714 mRNA. Translation: AAH81714.1 .
    RefSeqi NP_071782.1. NM_022387.3.
    XP_006243019.1. XM_006242957.1.
    XP_006243020.1. XM_006242958.1.
    UniGenei Rn.24751.

    3D structure databases

    ProteinModelPortali O35264.
    SMRi O35264. Positions 6-217.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35264. 2 interactions.
    STRINGi 10116.ENSRNOP00000024828.

    PTM databases

    PhosphoSitei O35264.

    Proteomic databases

    PaxDbi O35264.
    PRIDEi O35264.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024828 ; ENSRNOP00000024828 ; ENSRNOG00000018032 .
    GeneIDi 64189.
    KEGGi rno:64189.
    UCSCi RGD:620332. rat.

    Organism-specific databases

    CTDi 5049.
    RGDi 620332. Pafah1b2.

    Phylogenomic databases

    eggNOGi NOG69837.
    GeneTreei ENSGT00390000016520.
    HOGENOMi HOG000232143.
    HOVERGENi HBG053477.
    InParanoidi O35264.
    KOi K16795.
    OMAi NIRPKVV.
    OrthoDBi EOG7HB5BS.
    PhylomeDBi O35264.
    TreeFami TF323955.

    Miscellaneous databases

    NextBioi 612844.
    PROi O35264.

    Gene expression databases

    Genevestigatori O35264.

    Family and domain databases

    Gene3Di 3.40.50.1110. 1 hit.
    InterProi IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of cDNAs encoding alpha1, alpha2, and beta subunits of rat brain platelet-activating factor acetylhydrolase."
      Watanabe M., Aoki J., Manya H., Arai H., Inoue K.
      Biochim. Biophys. Acta 1401:73-79(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. Lubec G., Chen W.-Q., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 61-79 AND 134-142, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain and Hippocampus.

    Entry informationi

    Entry nameiPA1B2_RAT
    AccessioniPrimary (citable) accession number: O35264
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3