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O35263 (PA1B3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Platelet-activating factor acetylhydrolase IB subunit gamma
EC=3.1.1.47
Alternative name(s):
PAF acetylhydrolase 29 kDa subunit
Short name=PAF-AH 29 kDa subunit
PAF-AH subunit gamma
Short name=PAFAH subunit gamma
Platelet-activating factor acetylhydrolase alpha 1 subunit
Short name=PAF-AH alpha 1
Gene names
Name:Pafah1b3
Synonyms:Pafahg
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.

Catalytic activity

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Subunit structure

Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Expressed in brain, spleen, lung, liver, kidney and testis. Not expressed in heart and skeletal muscle.

Sequence similarities

Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 232232Platelet-activating factor acetylhydrolase IB subunit gamma
PRO_0000058157

Sites

Active site481 By similarity
Active site1931 By similarity
Active site1961 By similarity

Sequences

Sequence LengthMass (Da)Tools
O35263 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 5FEC63E2A611670B

FASTA23225,863
        10         20         30         40         50         60 
MSGEGENPAS KPTPVQDVQG DGRWMSLHHR FVADSKDKEP EVVFIGDSLV QLMHQCEIWR 

        70         80         90        100        110        120 
ELFSPLHALN FGIGGDSTQH VLWRLENGEL EHIRPKIVVV WVGTNNHSHT AEQVTGGIKA 

       130        140        150        160        170        180 
IVQLVNKLQP QARVVVLGLL PRGQHPNPLR EKNRQVNELV RAALAGYPRA HFLDADPGFV 

       190        200        210        220        230 
HSDGTISHHD MYDYLHLSRL GYTPVCRALH SLLLRLLAQD QGQGIPLPET AP

« Hide

References

[1]"Molecular cloning of cDNAs encoding alpha1, alpha2, and beta subunits of rat brain platelet-activating factor acetylhydrolase."
Watanabe M., Aoki J., Manya H., Arai H., Inoue K.
Biochim. Biophys. Acta 1401:73-79(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF016047 mRNA. Translation: AAC27973.1.
IPIIPI00207333.
RefSeqNP_446106.1. NM_053654.2.
UniGeneRn.17971.

3D structure databases

ProteinModelPortalO35263.
SMRO35263. Positions 6-216.
ModBaseSearch...

Protein-protein interaction databases

IntActO35263. 1 interaction.
STRING10116.ENSRNOP00000027774.

Proteomic databases

PaxDbO35263.
PRIDEO35263.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027774; ENSRNOP00000027774; ENSRNOG00000020481.
GeneID114113.
KEGGrno:114113.
UCSCRGD:620333. rat.

Organism-specific databases

CTD5050.
RGD620333. Pafah1b3.

Phylogenomic databases

eggNOGNOG69837.
GeneTreeENSGT00390000016520.
HOGENOMHOG000232143.
HOVERGENHBG053477.
InParanoidO35263.
KOK16795.
OMAFAPLHCL.
OrthoDBEOG45QHF4.

Gene expression databases

GenevestigatorO35263.
GermOnlineENSRNOG00000020481. Rattus norvegicus.

Family and domain databases

Gene3D3.40.50.1110. 1 hit.
InterProIPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PROSITEPS01098. LIPASE_GDSL_SER. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio618305.

Entry information

Entry namePA1B3_RAT
AccessionPrimary (citable) accession number: O35263
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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