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O35261 (E2F3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor E2F3

Short name=E2F-3
Gene names
Name:E2f3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner.

Subunit structure

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP. Ref.4

Subcellular location

Nucleus.

Developmental stage

In the developing nervous system, high levels expressed in both ventral and dorsal regions of the spinal cord from 13.5 dpc. Also expressed in dorsal root and cranial ganglia in 11.5-18.5 dpc embryos. Only low levels of expression in developing brain. In the developing retina (15.5 dpc), expression of E2F3 is localized to the ganglion cell layer. In other developing tissues, expressed in liver, lung and heart. Weak expression in developing kidney and skeletal muscle. Absent from the developing choroid plexus, thymus and developing skin. Low levels of expression in the developing intestinal epithelium and mesenchyme in 12.5-18.5 dpc embryos. Ref.3

Sequence similarities

Belongs to the E2F/DP family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457Transcription factor E2F3
PRO_0000219467

Regions

DNA binding147 – 23791 Potential
Region96 – 14550Cyclin A/CDK2 binding Potential
Region196 – 21722Leucine-zipper
Region238 – 32992Dimerization Potential
Region383 – 45775Transactivation Potential
Region424 – 44118Retinoblastoma protein binding Potential
Motif201 – 23737DEF box
Compositional bias22 – 4928Ala-rich
Compositional bias115 – 1217Poly-Gly

Experimental info

Sequence conflict102 – 1098SSRVGLLQ → CSSPTLLE in AAB71671. Ref.2
Sequence conflict3091V → L in AAB71671. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O35261 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: 076AC9F360101075

FASTA45748,757
        10         20         30         40         50         60 
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAP GTYIQILTTN 

        70         80         90        100        110        120 
PSTTSCATSL QSGALTAGPL LPSVPGTEPA ASSLYTTPQG PSSRVGLLQQ PPAPGRGGGG 

       130        140        150        160        170        180 
GPPAKRRLEL GESGHQYLSD GLKTPKGKGR AALRSPDSPK TPKSPSEKTR YDTSLGLLTK 

       190        200        210        220        230        240 
KFIQLLSQSP DGVLDLNKAA EVLKVQKRRI YDITNVLEGI HLIKKKSKNN VQWMGCSLSE 

       250        260        270        280        290        300 
DGGMLAQCQG LSKEVTELSQ EEKKLDELIQ SCTLDLKLLT EDSENQRLAY VTYQDIRKIS 

       310        320        330        340        350        360 
GLKDQTVIVV KAPPETRLEV PDSIESLQIH LASTQGPIEV YLCPEETETH RPMKTNNQDH 

       370        380        390        400        410        420 
NGNIPKPTSK DLASNNSGHS DCSVSTANLS PLASPANLLQ QTEDQIPSNL EGPFVNLLPP 

       430        440        450 
LLQEDYLLSL GEEEGISDLF DAYDLEKLPL VEDFMCS 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"Expression patterns of the E2F family of transcription factors during mouse nervous system development."
Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 102-457.
Strain: Swiss albino.
Tissue: Fibroblast.
[3]"Expression patterns of the E2F family of transcription factors during murine epithelial development."
Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[4]"EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EAPP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL513025 Genomic DNA. Translation: CAI24679.1.
AF015948 mRNA. Translation: AAB71671.1.
CCDSCCDS26413.1.
RefSeqNP_034223.1. NM_010093.3.
UniGeneMm.268356.

3D structure databases

ProteinModelPortalO35261.
SMRO35261. Positions 169-233, 249-346.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199351. 2 interactions.
DIPDIP-59314N.

PTM databases

PhosphoSiteO35261.

Proteomic databases

PRIDEO35261.

Protocols and materials databases

DNASU13557.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102948; ENSMUSP00000100012; ENSMUSG00000016477.
GeneID13557.
KEGGmmu:13557.
UCSCuc007pyq.2. mouse.

Organism-specific databases

CTD1871.
MGIMGI:1096340. E2f3.

Phylogenomic databases

eggNOGNOG289227.
HOGENOMHOG000232045.
HOVERGENHBG002227.
InParanoidO35261.
KOK06620.
OMALLYTTPH.
OrthoDBEOG738058.
PhylomeDBO35261.
TreeFamTF105566.

Gene expression databases

ArrayExpressO35261.
BgeeO35261.
CleanExMM_E2F3.
GenevestigatorO35261.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR12081. PTHR12081. 1 hit.
PfamPF02319. E2F_TDP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284174.
PROO35261.
SOURCESearch...

Entry information

Entry nameE2F3_MOUSE
AccessionPrimary (citable) accession number: O35261
Secondary accession number(s): Q5T0I6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 30, 2005
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot