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O35261

- E2F3_MOUSE

UniProt

O35261 - E2F3_MOUSE

Protein

Transcription factor E2F3

Gene

E2f3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi147 – 23791Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. core promoter binding Source: Ensembl
    2. DNA binding Source: MGI
    3. protein binding Source: MGI
    4. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. positive regulation of cell proliferation Source: MGI
    3. positive regulation of transcription, DNA-templated Source: MGI
    4. regulation of transcription, DNA-templated Source: MGI
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_227891. G2 Phase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor E2F3
    Short name:
    E2F-3
    Gene namesi
    Name:E2f3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1096340. E2f3.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 457457Transcription factor E2F3PRO_0000219467Add
    BLAST

    Proteomic databases

    PRIDEiO35261.

    PTM databases

    PhosphoSiteiO35261.

    Expressioni

    Developmental stagei

    In the developing nervous system, high levels expressed in both ventral and dorsal regions of the spinal cord from 13.5 dpc. Also expressed in dorsal root and cranial ganglia in 11.5-18.5 dpc embryos. Only low levels of expression in developing brain. In the developing retina (15.5 dpc), expression of E2F3 is localized to the ganglion cell layer. In other developing tissues, expressed in liver, lung and heart. Weak expression in developing kidney and skeletal muscle. Absent from the developing choroid plexus, thymus and developing skin. Low levels of expression in the developing intestinal epithelium and mesenchyme in 12.5-18.5 dpc embryos.1 Publication

    Gene expression databases

    ArrayExpressiO35261.
    BgeeiO35261.
    CleanExiMM_E2F3.
    GenevestigatoriO35261.

    Interactioni

    Subunit structurei

    Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.1 Publication

    Protein-protein interaction databases

    BioGridi199351. 2 interactions.
    DIPiDIP-59314N.

    Structurei

    3D structure databases

    ProteinModelPortaliO35261.
    SMRiO35261. Positions 169-233, 249-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni96 – 14550Cyclin A/CDK2 bindingSequence AnalysisAdd
    BLAST
    Regioni196 – 21722Leucine-zipperAdd
    BLAST
    Regioni238 – 32992DimerizationSequence AnalysisAdd
    BLAST
    Regioni383 – 45775TransactivationSequence AnalysisAdd
    BLAST
    Regioni424 – 44118Retinoblastoma protein bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi201 – 23737DEF boxAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 4928Ala-richAdd
    BLAST
    Compositional biasi115 – 1217Poly-Gly

    Sequence similaritiesi

    Belongs to the E2F/DP family.Curated

    Phylogenomic databases

    eggNOGiNOG289227.
    HOGENOMiHOG000232045.
    HOVERGENiHBG002227.
    InParanoidiO35261.
    KOiK06620.
    OMAiLLYTTPH.
    OrthoDBiEOG738058.
    PhylomeDBiO35261.
    TreeFamiTF105566.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR015633. E2F.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR12081. PTHR12081. 1 hit.
    PfamiPF02319. E2F_TDP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O35261-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAP    50
    GTYIQILTTN PSTTSCATSL QSGALTAGPL LPSVPGTEPA ASSLYTTPQG 100
    PSSRVGLLQQ PPAPGRGGGG GPPAKRRLEL GESGHQYLSD GLKTPKGKGR 150
    AALRSPDSPK TPKSPSEKTR YDTSLGLLTK KFIQLLSQSP DGVLDLNKAA 200
    EVLKVQKRRI YDITNVLEGI HLIKKKSKNN VQWMGCSLSE DGGMLAQCQG 250
    LSKEVTELSQ EEKKLDELIQ SCTLDLKLLT EDSENQRLAY VTYQDIRKIS 300
    GLKDQTVIVV KAPPETRLEV PDSIESLQIH LASTQGPIEV YLCPEETETH 350
    RPMKTNNQDH NGNIPKPTSK DLASNNSGHS DCSVSTANLS PLASPANLLQ 400
    QTEDQIPSNL EGPFVNLLPP LLQEDYLLSL GEEEGISDLF DAYDLEKLPL 450
    VEDFMCS 457
    Length:457
    Mass (Da):48,757
    Last modified:August 30, 2005 - v2
    Checksum:i076AC9F360101075
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1098SSRVGLLQ → CSSPTLLE in AAB71671. (PubMed:9376316)Curated
    Sequence conflicti309 – 3091V → L in AAB71671. (PubMed:9376316)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL513025 Genomic DNA. Translation: CAI24679.1.
    AF015948 mRNA. Translation: AAB71671.1.
    CCDSiCCDS26413.1.
    RefSeqiNP_034223.1. NM_010093.3.
    UniGeneiMm.268356.

    Genome annotation databases

    EnsembliENSMUST00000102948; ENSMUSP00000100012; ENSMUSG00000016477.
    GeneIDi13557.
    KEGGimmu:13557.
    UCSCiuc007pyq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL513025 Genomic DNA. Translation: CAI24679.1 .
    AF015948 mRNA. Translation: AAB71671.1 .
    CCDSi CCDS26413.1.
    RefSeqi NP_034223.1. NM_010093.3.
    UniGenei Mm.268356.

    3D structure databases

    ProteinModelPortali O35261.
    SMRi O35261. Positions 169-233, 249-346.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199351. 2 interactions.
    DIPi DIP-59314N.

    PTM databases

    PhosphoSitei O35261.

    Proteomic databases

    PRIDEi O35261.

    Protocols and materials databases

    DNASUi 13557.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102948 ; ENSMUSP00000100012 ; ENSMUSG00000016477 .
    GeneIDi 13557.
    KEGGi mmu:13557.
    UCSCi uc007pyq.2. mouse.

    Organism-specific databases

    CTDi 1871.
    MGIi MGI:1096340. E2f3.

    Phylogenomic databases

    eggNOGi NOG289227.
    HOGENOMi HOG000232045.
    HOVERGENi HBG002227.
    InParanoidi O35261.
    KOi K06620.
    OMAi LLYTTPH.
    OrthoDBi EOG738058.
    PhylomeDBi O35261.
    TreeFami TF105566.

    Enzyme and pathway databases

    Reactomei REACT_227891. G2 Phase.

    Miscellaneous databases

    NextBioi 284174.
    PROi O35261.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35261.
    Bgeei O35261.
    CleanExi MM_E2F3.
    Genevestigatori O35261.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR015633. E2F.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR12081. PTHR12081. 1 hit.
    Pfami PF02319. E2F_TDP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
      Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
      Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 102-457.
      Strain: Swiss albino.
      Tissue: Fibroblast.
    3. "Expression patterns of the E2F family of transcription factors during murine epithelial development."
      Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
      Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    4. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
      Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
      Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EAPP.

    Entry informationi

    Entry nameiE2F3_MOUSE
    AccessioniPrimary (citable) accession number: O35261
    Secondary accession number(s): Q5T0I6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3