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Protein

Transcription factor E2F3

Gene

E2f3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (PubMed:20176812).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi147 – 23791Sequence AnalysisAdd
BLAST

GO - Molecular functioni

  • core promoter binding Source: MGI
  • DNA binding Source: MGI
  • sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • negative regulation of fat cell proliferation Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_283368. G2 Phase.
REACT_289448. Cyclin D associated events in G1.
REACT_297337. CDC6 association with the ORC:origin complex.
REACT_349855. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F3
Short name:
E2F-3
Gene namesi
Name:E2f3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1096340. E2f3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Transcription factor E2F3PRO_0000219467Add
BLAST

Proteomic databases

MaxQBiO35261.
PRIDEiO35261.

PTM databases

PhosphoSiteiO35261.

Expressioni

Developmental stagei

In the developing nervous system, high levels expressed in both ventral and dorsal regions of the spinal cord from 13.5 dpc. Also expressed in dorsal root and cranial ganglia in 11.5-18.5 dpc embryos. Only low levels of expression in developing brain. In the developing retina (15.5 dpc), expression of E2F3 is localized to the ganglion cell layer. In other developing tissues, expressed in liver, lung and heart. Weak expression in developing kidney and skeletal muscle. Absent from the developing choroid plexus, thymus and developing skin. Low levels of expression in the developing intestinal epithelium and mesenchyme in 12.5-18.5 dpc embryos.1 Publication

Gene expression databases

BgeeiO35261.
CleanExiMM_E2F3.
ExpressionAtlasiO35261. baseline and differential.
GenevisibleiO35261. MM.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.1 Publication

Protein-protein interaction databases

BioGridi199351. 2 interactions.
DIPiDIP-59314N.
STRINGi10090.ENSMUSP00000100012.

Structurei

3D structure databases

ProteinModelPortaliO35261.
SMRiO35261. Positions 169-233, 249-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni96 – 14550Cyclin A/CDK2 bindingSequence AnalysisAdd
BLAST
Regioni196 – 21722Leucine-zipperAdd
BLAST
Regioni238 – 32992DimerizationSequence AnalysisAdd
BLAST
Regioni383 – 45775TransactivationSequence AnalysisAdd
BLAST
Regioni424 – 44118Retinoblastoma protein bindingSequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi201 – 23737DEF boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 4928Ala-richAdd
BLAST
Compositional biasi115 – 1217Poly-Gly

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiNOG289227.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiO35261.
KOiK06620.
OMAiLLYTTPH.
OrthoDBiEOG738058.
PhylomeDBiO35261.
TreeFamiTF105566.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF02319. E2F_TDP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAP
60 70 80 90 100
GTYIQILTTN PSTTSCATSL QSGALTAGPL LPSVPGTEPA ASSLYTTPQG
110 120 130 140 150
PSSRVGLLQQ PPAPGRGGGG GPPAKRRLEL GESGHQYLSD GLKTPKGKGR
160 170 180 190 200
AALRSPDSPK TPKSPSEKTR YDTSLGLLTK KFIQLLSQSP DGVLDLNKAA
210 220 230 240 250
EVLKVQKRRI YDITNVLEGI HLIKKKSKNN VQWMGCSLSE DGGMLAQCQG
260 270 280 290 300
LSKEVTELSQ EEKKLDELIQ SCTLDLKLLT EDSENQRLAY VTYQDIRKIS
310 320 330 340 350
GLKDQTVIVV KAPPETRLEV PDSIESLQIH LASTQGPIEV YLCPEETETH
360 370 380 390 400
RPMKTNNQDH NGNIPKPTSK DLASNNSGHS DCSVSTANLS PLASPANLLQ
410 420 430 440 450
QTEDQIPSNL EGPFVNLLPP LLQEDYLLSL GEEEGISDLF DAYDLEKLPL

VEDFMCS
Length:457
Mass (Da):48,757
Last modified:August 30, 2005 - v2
Checksum:i076AC9F360101075
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1098SSRVGLLQ → CSSPTLLE in AAB71671 (PubMed:9376316).Curated
Sequence conflicti309 – 3091V → L in AAB71671 (PubMed:9376316).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513025 Genomic DNA. Translation: CAI24679.1.
AF015948 mRNA. Translation: AAB71671.1.
CCDSiCCDS26413.1.
RefSeqiNP_034223.1. NM_010093.3.
UniGeneiMm.268356.

Genome annotation databases

EnsembliENSMUST00000102948; ENSMUSP00000100012; ENSMUSG00000016477.
GeneIDi13557.
KEGGimmu:13557.
UCSCiuc007pyq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513025 Genomic DNA. Translation: CAI24679.1.
AF015948 mRNA. Translation: AAB71671.1.
CCDSiCCDS26413.1.
RefSeqiNP_034223.1. NM_010093.3.
UniGeneiMm.268356.

3D structure databases

ProteinModelPortaliO35261.
SMRiO35261. Positions 169-233, 249-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199351. 2 interactions.
DIPiDIP-59314N.
STRINGi10090.ENSMUSP00000100012.

PTM databases

PhosphoSiteiO35261.

Proteomic databases

MaxQBiO35261.
PRIDEiO35261.

Protocols and materials databases

DNASUi13557.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102948; ENSMUSP00000100012; ENSMUSG00000016477.
GeneIDi13557.
KEGGimmu:13557.
UCSCiuc007pyq.2. mouse.

Organism-specific databases

CTDi1871.
MGIiMGI:1096340. E2f3.

Phylogenomic databases

eggNOGiNOG289227.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiO35261.
KOiK06620.
OMAiLLYTTPH.
OrthoDBiEOG738058.
PhylomeDBiO35261.
TreeFamiTF105566.

Enzyme and pathway databases

ReactomeiREACT_283368. G2 Phase.
REACT_289448. Cyclin D associated events in G1.
REACT_297337. CDC6 association with the ORC:origin complex.
REACT_349855. Association of licensing factors with the pre-replicative complex.

Miscellaneous databases

ChiTaRSiE2f3. mouse.
NextBioi284174.
PROiO35261.
SOURCEiSearch...

Gene expression databases

BgeeiO35261.
CleanExiMM_E2F3.
ExpressionAtlasiO35261. baseline and differential.
GenevisibleiO35261. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF02319. E2F_TDP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 102-457.
    Strain: Swiss albino.
    Tissue: Fibroblast.
  3. "Expression patterns of the E2F family of transcription factors during murine epithelial development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  4. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
    Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
    Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EAPP.
  5. "Repression of transcriptional activity of C/EBPalpha by E2F-dimerization partner complexes."
    Zaragoza K., Begay V., Schuetz A., Heinemann U., Leutz A.
    Mol. Cell. Biol. 30:2293-2304(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiE2F3_MOUSE
AccessioniPrimary (citable) accession number: O35261
Secondary accession number(s): Q5T0I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 30, 2005
Last modified: June 24, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.