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Protein

Nucleus accumbens-associated protein 1

Gene

Nacc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcriptional repressor. Isoform 1 is a stronger transcriptional repressor than isoform 2. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.4 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleus accumbens-associated protein 1
Short name:
NAC-1
Alternative name(s):
BTB/POZ domain-containing protein 14B
Gene namesi
Name:Nacc1
Synonyms:Btbd14b, Nac1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi621003. Nacc1.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Punctate distribution in nucleus. In differentiated PC12 cells diffusely distributed in the cytoplasm and is dependent on phosphorylation.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231Q → L: Abrogates transcriptional repression. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Nucleus accumbens-associated protein 1PRO_0000274043Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki167 – 167Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki167 – 167Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei187 – 1871PhosphoserineBy similarity
Modified residuei245 – 2451Phosphoserine; by PKC1 Publication
Modified residuei492 – 4921PhosphoserineBy similarity
Modified residuei496 – 4961PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by protein kinase C (PKC).1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO35260.
PRIDEiO35260.

PTM databases

iPTMnetiO35260.
PhosphoSiteiO35260.

Expressioni

Tissue specificityi

Highly expressed in the hippocampus, brain cortex, cerebellum and brainstem. Expressed in the nucleus accumbens, olfactory tubercle, the striatum, frontal and parietal cortex and ventral pallidum. Weakly expressed in the heart, liver, kidney, spleen, testis, and skeletal muscle. Isoform 2 is expressed in the brain and liver, less abundantly expressed in the brain than isoform 1.1 Publication

Developmental stagei

Expressed in the brain at E16, E18, P2, P8 and P90.2 Publications

Inductioni

Expression is increased by cocaine, selectively in the nucleus accumbens. mRNA is present at increased levels in the nucleus accumbens 3 weeks after withdrawal from cocaine self-administration.1 Publication

Gene expression databases

GenevisibleiO35260. RN.

Interactioni

Subunit structurei

Homooligomer; mediated by the BTB domain (By similarity). Both isoforms interact with HDAC3 and HDAC4. Interacts (via BTB domain) with CUL3, PSMD7 AND RCOR1.By similarity3 Publications

Protein-protein interaction databases

BioGridi251261. 1 interaction.
STRINGi10116.ENSRNOP00000003884.

Structurei

3D structure databases

ProteinModelPortaliO35260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 9465BTBPROSITE-ProRule annotationAdd
BLAST
Domaini360 – 45798BENPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BEN domain.PROSITE-ProRule annotation
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000119063.
HOGENOMiHOG000231834.
HOVERGENiHBG052875.
InParanoidiO35260.
KOiK10486.
OMAiNMGDQFL.
OrthoDBiEOG7WT419.
PhylomeDBiO35260.

Family and domain databases

InterProiIPR018379. BEN_domain.
IPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF10523. BEN. 1 hit.
PF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM01025. BEN. 1 hit.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS51457. BEN. 1 hit.
PS50097. BTB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35260-1) [UniParc]FASTAAdd to basket

Also known as: lNAC1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQTLQMEIP NFGNSILECL NEQRLQGLYC DVSVVVKGHA FKAHRAVLAA
60 70 80 90 100
SSSYFRDLFN SSRSAVVELP AAVQPQSFQQ ILTFCYTGRL SMNMGDQFLL
110 120 130 140 150
IYTAGFLQIQ EIMEKGTEFF LKVSSPSCDS QGLHPEEAPS SEPQSPVAQI
160 170 180 190 200
LGWPACSTPL PLVSRVKTEQ ELDSVQCTPM AKRLWDSSQK EAGGSGGNNG
210 220 230 240 250
SRKMAKFSTP DLAPNRMPQP VSVATATAAV AVVAVGGCVS GPSMSERTSP
260 270 280 290 300
GTSSAYTSDS PSSYHNEEDE EEDAGEEGTD EQYRQICNMY TMYSMLNVGQ
310 320 330 340 350
TVEKVEALPE QVVLESHSRI RVRQDLASLP AELINQIGNR CHPKLYDEGD
360 370 380 390 400
PSEKLELVTG TNVYITRAQL MNCHVSAGTR HKVLLRRLLA SFFDRNTLAN
410 420 430 440 450
SCGTGIRSST NDPRRKPLDS RVLHAVKYYC QNFAPNFKES EMNAIAADMC
460 470 480 490 500
TNARRVVRKS WLPKTKPLHL VEGDNYSSFI SDTGKIEPDM MSMEHSFETA
510
SHDGEAGPSA EVLQ
Length:514
Mass (Da):56,450
Last modified:January 1, 1998 - v1
Checksum:i5980514174B4BFF5
GO
Isoform 2 (identifier: O35260-2) [UniParc]FASTAAdd to basket

Also known as: sNAC1

The sequence of this isoform differs from the canonical sequence as follows:
     275-301: Missing.

Show »
Length:487
Mass (Da):53,305
Checksum:iC9B61758169AA895
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei275 – 30127Missing in isoform 2. CuratedVSP_022613Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015911 mRNA. Translation: AAB69864.1.
RefSeqiNP_599240.1. NM_134413.2. [O35260-1]
XP_008770568.1. XM_008772346.1. [O35260-1]
UniGeneiRn.108186.

Genome annotation databases

EnsembliENSRNOT00000003884; ENSRNOP00000003884; ENSRNOG00000002864. [O35260-1]
ENSRNOT00000091670; ENSRNOP00000071927; ENSRNOG00000002864. [O35260-1]
GeneIDi171454.
KEGGirno:171454.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015911 mRNA. Translation: AAB69864.1.
RefSeqiNP_599240.1. NM_134413.2. [O35260-1]
XP_008770568.1. XM_008772346.1. [O35260-1]
UniGeneiRn.108186.

3D structure databases

ProteinModelPortaliO35260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251261. 1 interaction.
STRINGi10116.ENSRNOP00000003884.

PTM databases

iPTMnetiO35260.
PhosphoSiteiO35260.

Proteomic databases

PaxDbiO35260.
PRIDEiO35260.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003884; ENSRNOP00000003884; ENSRNOG00000002864. [O35260-1]
ENSRNOT00000091670; ENSRNOP00000071927; ENSRNOG00000002864. [O35260-1]
GeneIDi171454.
KEGGirno:171454.

Organism-specific databases

CTDi112939.
RGDi621003. Nacc1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000119063.
HOGENOMiHOG000231834.
HOVERGENiHBG052875.
InParanoidiO35260.
KOiK10486.
OMAiNMGDQFL.
OrthoDBiEOG7WT419.
PhylomeDBiO35260.

Miscellaneous databases

PROiO35260.

Gene expression databases

GenevisibleiO35260. RN.

Family and domain databases

InterProiIPR018379. BEN_domain.
IPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF10523. BEN. 1 hit.
PF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM01025. BEN. 1 hit.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS51457. BEN. 1 hit.
PS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "NAC-1, a rat brain mRNA, is increased in the nucleus accumbens three weeks after chronic cocaine self-administration."
    Cha X.-Y., Pierce R.C., Kalivas P.W., Mackler S.A.
    J. Neurosci. 17:6864-6871(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Differences in expression, actions and cocaine regulation of two isoforms for the brain transcriptional regulator NAC1."
    Korutla L., Wang P.J., Lewis D.M., Neustadter J.H., Stromberg M.F., Mackler S.A.
    Neuroscience 110:421-429(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, ALTERNATIVE SPLICING.
  3. "The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal-like cultures."
    Korutla L., Wang P.J., Mackler S.A.
    J. Neurochem. 94:786-793(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HDAC3 AND HDAC4.
  4. Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-245.
  5. "NAC1, a cocaine-regulated POZ/BTB protein interacts with CoREST."
    Korutla L., Degnan R., Wang P., Mackler S.A.
    J. Neurochem. 101:611-618(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RCOR1, MUTAGENESIS OF GLN-23.
  6. "NAC1 regulates the recruitment of the proteasome complex into dendritic spines."
    Shen H., Korutla L., Champtiaux N., Toda S., LaLumiere R., Vallone J., Klugmann M., Blendy J.A., Mackler S.A., Kalivas P.W.
    J. Neurosci. 27:8903-8913(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CUL3 AND PSMD7, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNACC1_RAT
AccessioniPrimary (citable) accession number: O35260
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.