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Protein

Golgi reassembly-stacking protein 1

Gene

Gorasp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes.2 Publications

GO - Biological processi

  • Golgi organization Source: UniProtKB
  • negative regulation of dendrite morphogenesis Source: UniProtKB
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 1
Alternative name(s):
Golgi peripheral membrane protein p65
Golgi reassembly-stacking protein of 65 kDa1 Publication
Short name:
GRASP651 Publication
Gene namesi
Name:Gorasp1
Synonyms:Grasp651 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621122. Gorasp1.

Subcellular locationi

  • Golgi apparatuscis-Golgi network membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

  • Note: Undergoes rapid exchange with the cytosol.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi320 – 3201D → A: Blocks the caspase-mediated cleavage. 1 Publication
Mutagenesisi375 – 3751D → A: Blocks the caspase-mediated cleavage. 1 Publication
Mutagenesisi393 – 3931D → A: Blocks the caspase-mediated cleavage. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCurated
Chaini2 – 451450Golgi reassembly-stacking protein 1PRO_0000087572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Modified residuei216 – 2161PhosphoserineCombined sources
Modified residuei220 – 2201PhosphothreonineCombined sources
Modified residuei224 – 2241PhosphothreonineBy similarity
Modified residuei365 – 3651PhosphoserineCombined sources
Modified residuei367 – 3671PhosphoserineCombined sources
Modified residuei376 – 3761PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking (By similarity).By similarity
Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiO35254.
PRIDEiO35254.

PTM databases

iPTMnetiO35254.
PhosphoSiteiO35254.

Interactioni

Subunit structurei

Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GOLGA2/GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. The complex binds to the vesicle docking protein p115/USO1. Interacts with TMED2 and TMED3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Golga2Q628392EBI-4422879,EBI-618335
Tmed2Q635243EBI-4422879,EBI-918600

Protein-protein interaction databases

DIPiDIP-60188N.
IntActiO35254. 3 interactions.
MINTiMINT-4578765.
STRINGi10116.ENSRNOP00000024408.

Structurei

Secondary structure

1
451
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 216Combined sources
Helixi26 – 294Combined sources
Turni34 – 363Combined sources
Beta strandi37 – 426Combined sources
Beta strandi49 – 524Combined sources
Helixi53 – 608Combined sources
Turni61 – 633Combined sources
Beta strandi66 – 727Combined sources
Turni73 – 753Combined sources
Beta strandi78 – 836Combined sources
Beta strandi89 – 957Combined sources
Beta strandi97 – 1015Combined sources
Helixi104 – 1096Combined sources
Beta strandi112 – 1176Combined sources
Helixi122 – 1265Combined sources
Turni130 – 1323Combined sources
Beta strandi133 – 1397Combined sources
Helixi148 – 1547Combined sources
Turni155 – 1573Combined sources
Beta strandi160 – 1667Combined sources
Turni167 – 1704Combined sources
Beta strandi171 – 1777Combined sources
Beta strandi183 – 1897Combined sources
Beta strandi191 – 1944Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KFVX-ray2.20A1-210[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7470PDZAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 20113Essential for the interaction with GOLGA2/GM130Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi298 – 3014Poly-Pro
Compositional biasi314 – 35542Ser-richAdd
BLAST
Compositional biasi351 – 3555Poly-Ser

Sequence similaritiesi

Belongs to the GORASP family.Curated
Contains 1 PDZ (DHR) domain.Curated

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
HOGENOMiHOG000054196.
HOVERGENiHBG051826.
InParanoidiO35254.
PhylomeDBiO35254.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLGASSEQP AGGEGFHLHG VQENSPAQQA GLEPYFDFII TIGHSRLNKE
60 70 80 90 100
NDTLKALLKA NVEKPVKLEV FNMKTMRVRE VEVVPSNMWG GQGLLGASVR
110 120 130 140 150
FCSFRRASEH VWHVLDVEPS SPAALAGLRP YTDYIVGSDQ ILQESEDFFT
160 170 180 190 200
LIESHEGKPL KLMVYNSESD SCREVTVTPN AAWGGEGSLG CGIGYGYLHR
210 220 230 240 250
IPTQPSSQYK KPPSASSPGT PAKTPQPNAF PLGAPPPWPI PQDSSGPELG
260 270 280 290 300
SRQSDYMEAL PQVPGGFMEE QLPGPGSPGH GTADYGGCLH SMEIPLQPPP
310 320 330 340 350
PVQRVMDPGF LDVSGMSLLD SNNTSVCPSL SSSSLLTPTA VSALGPEDIG
360 370 380 390 400
SSSSSHERGG EATWSGSEFE ISFPDSPGSQ AQVDHLPRLT LPDGLTSAAS
410 420 430 440 450
PEEGLSAELL EAQTEEPAHT ASLDCMAQTE GPAGQVQAAP DPEPGLCEGP

W
Length:451
Mass (Da):47,673
Last modified:January 23, 2007 - v4
Checksum:i5B38FADF2E255EA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015264 mRNA. Translation: AAB81355.2.
UniGeneiRn.144458.

Genome annotation databases

UCSCiRGD:621122. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015264 mRNA. Translation: AAB81355.2.
UniGeneiRn.144458.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KFVX-ray2.20A1-210[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60188N.
IntActiO35254. 3 interactions.
MINTiMINT-4578765.
STRINGi10116.ENSRNOP00000024408.

PTM databases

iPTMnetiO35254.
PhosphoSiteiO35254.

Proteomic databases

PaxDbiO35254.
PRIDEiO35254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621122. rat.

Organism-specific databases

RGDi621122. Gorasp1.

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
HOGENOMiHOG000054196.
HOVERGENiHBG051826.
InParanoidiO35254.
PhylomeDBiO35254.

Miscellaneous databases

PROiO35254.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GRASP65, a protein involved in the stacking of Golgi cisternae."
    Barr F.A., Puype M., Vandekerckhove J., Warren G.
    Cell 91:253-262(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GOLGA2, PHOSPHORYLATION, MYRISTOYLATION AT GLY-2.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. Barr F.A.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus."
    Barr F.A., Preisinger C., Kopajtich R., Koerner R.
    J. Cell Biol. 155:885-891(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TMED2 AND TMED3.
  4. "Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis."
    Lane J.D., Lucocq J., Pryde J., Barr F.A., Woodman P.G., Allan V.J., Lowe M.
    J. Cell Biol. 156:495-509(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CASPASE-MEDIATED CLEAVAGE, MUTAGENESIS OF ASP-320; ASP-375 AND ASP-393.
  5. "Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae."
    Barr F.A., Nakamura N., Warren G.
    EMBO J. 17:3258-3268(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GOLGA2.
  6. "Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells."
    Suetterlin C., Hsu P., Mallabiabarrena A., Malhotra V.
    Cell 109:359-369(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN GOLGI FRAGMENTATION.
  7. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; THR-220; SER-365 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGORS1_RAT
AccessioniPrimary (citable) accession number: O35254
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 106 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.