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O35250

- EXOC7_MOUSE

UniProt

O35250 - EXOC7_MOUSE

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Protein

Exocyst complex component 7

Gene

Exoc7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.1 Publication

GO - Biological processi

  1. exocytosis Source: UniProtKB-KW
  2. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.
REACT_207837. Insulin processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Exocyst complex component 7
Alternative name(s):
Exocyst complex component Exo70
Gene namesi
Name:Exoc7
Synonyms:Exo70
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1859270. Exoc7.

Subcellular locationi

Cytoplasmcytosol. Cell membrane; Peripheral membrane protein
Note: Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.

GO - Cellular componenti

  1. centriolar satellite Source: Ensembl
  2. exocyst Source: InterPro
  3. growth cone membrane Source: MGI
  4. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 697697Exocyst complex component 7PRO_0000118961Add
BLAST

Proteomic databases

MaxQBiO35250.
PaxDbiO35250.
PRIDEiO35250.

PTM databases

PhosphoSiteiO35250.

Expressioni

Gene expression databases

BgeeiO35250.
CleanExiMM_EXOC7.
ExpressionAtlasiO35250. baseline and differential.
GenevestigatoriO35250.

Interactioni

Subunit structurei

The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with RAB11FIP3 By similarity. Interacts with ARHQ in a GTP-dependent manner.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Exoc4O353822EBI-775332,EBI-772648

Protein-protein interaction databases

IntActiO35250. 7 interactions.
MINTiMINT-1897779.

Structurei

Secondary structure

1
697
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi89 – 913
Helixi93 – 10311
Helixi109 – 12921
Helixi134 – 16128
Helixi167 – 17610
Helixi193 – 20917
Helixi214 – 24027
Helixi307 – 33428
Helixi337 – 3393
Helixi340 – 37132
Turni377 – 3804
Helixi381 – 39818
Turni399 – 4013
Helixi404 – 4074
Helixi409 – 43426
Helixi439 – 4413
Helixi450 – 46415
Helixi466 – 4749
Helixi500 – 52627
Helixi532 – 54716
Turni548 – 5525
Helixi555 – 5606
Helixi566 – 58217
Helixi583 – 5853
Helixi586 – 5894
Turni590 – 5923
Helixi594 – 5963
Helixi608 – 63225
Helixi640 – 66627
Helixi674 – 6774
Helixi682 – 6909
Beta strandi692 – 6943

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PFTX-ray2.25A85-697[»]
ProteinModelPortaliO35250.
SMRiO35250. Positions 85-241, 306-696.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35250.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 384384SEC8 and ARHQ bindingAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili5 – 4238Sequence AnalysisAdd
BLAST
Coiled coili63 – 8523Sequence AnalysisAdd
BLAST

Domaini

The C-terminus is required for translocation to the plasma membrane.

Sequence similaritiesi

Belongs to the EXO70 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG245075.
HOGENOMiHOG000231194.
HOVERGENiHBG051515.
InParanoidiO35250.
KOiK07195.
OMAiADIIPEH.
OrthoDBiEOG7S4X64.
PhylomeDBiO35250.
TreeFamiTF324243.

Family and domain databases

InterProiIPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view]
PANTHERiPTHR12542. PTHR12542. 1 hit.
PfamiPF03081. Exo70. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O35250-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIPPQEASAR RREIEDKLKQ EEETLSFIRD SLEKSDQLTR NMVSILSSFE
60 70 80 90 100
SRLMKLENSI IPVHKQTENL QRLQENVEKT LSCLDHVISY YHVASDTEKI
110 120 130 140 150
IREGPTGRLE EYLGSMAKIQ KAVEYFQDNS PDSPELNKVK LLFERGKESL
160 170 180 190 200
ESEFRSLMTR HSKVVSPVLL LDLISADDEL EVQEDVVLEH LPESVLRDVV
210 220 230 240 250
RISRWLVEYG RNQDFMNVYY QIRSSQLDRS IKGLKEHFRK SSSSSGVPYS
260 270 280 290 300
PAIPNKRKDT PTKKPIKRPG TIRKAQNLLK QYSQHGLDGK KGGSNLIPLE
310 320 330 340 350
GRDDMLDVET DAYIHCVSAF VKLAQSEYRL LMEIIPEHHQ KKTFDSLIQD
360 370 380 390 400
ALDGLMLEGE NIVSAARKAI IRHDFSTVLT VFPILRHLKQ TKPEFDQVLQ
410 420 430 440 450
GTAASTKNKL PGLITSMETI GAKALEDFAD NIKNDPDKEY NMPKDGTVHE
460 470 480 490 500
LTSNAILFLQ QLLDFQETAG AMLASQVLGD TYNIPLDPRE TSSSATSYSS
510 520 530 540 550
EFSKRLLSTY ICKVLGNLQL NLLSKSKVYE DPALSAIFLH NNYNYILKSL
560 570 580 590 600
EKSELIQLVA VTQKTAERSY REHIEQQIQT YQRSWLKVTD YIAEKNLPVF
610 620 630 640 650
QPGVKLRDKE RQMIKERFKG FNDGLEELCK IQKVWAIPDT EQRDKIRQAQ
660 670 680 690
KDIVKETYGA FLHRYGSVPF TKNPEKYIKY RVEQVGDMID RLFDTSA
Length:697
Mass (Da):79,960
Last modified:December 6, 2002 - v2
Checksum:i0A78F00E1D5D575A
GO
Isoform 2 (identifier: O35250-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-300: Missing.
     477-489: Missing.

Show »
Length:653
Mass (Da):75,159
Checksum:i40BB7E7298E82D53
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401R → K in AAB69345. 1 PublicationCurated
Sequence conflicti165 – 1651V → I in AAB69345. 1 PublicationCurated
Sequence conflicti433 – 4331K → E in AAB69345. 1 PublicationCurated
Sequence conflicti529 – 5291Y → F in AAB69345. 1 PublicationCurated
Sequence conflicti610 – 6112ER → DP in AAB69345. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei270 – 30031Missing in isoform 2. 1 PublicationVSP_001484Add
BLAST
Alternative sequencei477 – 48913Missing in isoform 2. 1 PublicationVSP_001485Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF014461 mRNA. Translation: AAB69345.1.
BC028927 mRNA. Translation: AAH28927.1.
CCDSiCCDS25664.1. [O35250-1]
CCDS48983.1. [O35250-2]
PIRiT03722.
RefSeqiNP_001156344.1. NM_001162872.1. [O35250-2]
NP_058553.2. NM_016857.2. [O35250-1]
UniGeneiMm.22530.

Genome annotation databases

EnsembliENSMUST00000021147; ENSMUSP00000021147; ENSMUSG00000020792. [O35250-1]
ENSMUST00000106411; ENSMUSP00000102019; ENSMUSG00000020792. [O35250-2]
GeneIDi53413.
KEGGimmu:53413.
UCSCiuc007mks.2. mouse. [O35250-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF014461 mRNA. Translation: AAB69345.1 .
BC028927 mRNA. Translation: AAH28927.1 .
CCDSi CCDS25664.1. [O35250-1 ]
CCDS48983.1. [O35250-2 ]
PIRi T03722.
RefSeqi NP_001156344.1. NM_001162872.1. [O35250-2 ]
NP_058553.2. NM_016857.2. [O35250-1 ]
UniGenei Mm.22530.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2PFT X-ray 2.25 A 85-697 [» ]
ProteinModelPortali O35250.
SMRi O35250. Positions 85-241, 306-696.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O35250. 7 interactions.
MINTi MINT-1897779.

PTM databases

PhosphoSitei O35250.

Proteomic databases

MaxQBi O35250.
PaxDbi O35250.
PRIDEi O35250.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021147 ; ENSMUSP00000021147 ; ENSMUSG00000020792 . [O35250-1 ]
ENSMUST00000106411 ; ENSMUSP00000102019 ; ENSMUSG00000020792 . [O35250-2 ]
GeneIDi 53413.
KEGGi mmu:53413.
UCSCi uc007mks.2. mouse. [O35250-1 ]

Organism-specific databases

CTDi 23265.
MGIi MGI:1859270. Exoc7.

Phylogenomic databases

eggNOGi NOG245075.
HOGENOMi HOG000231194.
HOVERGENi HBG051515.
InParanoidi O35250.
KOi K07195.
OMAi ADIIPEH.
OrthoDBi EOG7S4X64.
PhylomeDBi O35250.
TreeFami TF324243.

Enzyme and pathway databases

Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.
REACT_207837. Insulin processing.

Miscellaneous databases

ChiTaRSi EXOC7. mouse.
EvolutionaryTracei O35250.
NextBioi 310225.
PROi O35250.
SOURCEi Search...

Gene expression databases

Bgeei O35250.
CleanExi MM_EXOC7.
ExpressionAtlasi O35250. baseline and differential.
Genevestigatori O35250.

Family and domain databases

InterProi IPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view ]
PANTHERi PTHR12542. PTHR12542. 1 hit.
Pfami PF03081. Exo70. 1 hit.
[Graphical view ]
SUPFAMi SSF74788. SSF74788. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Guo W., Roth D., De Camilli P., Novick P.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Colon.
  3. "The exocyst complex is required for targeting of Glut4 to the plasma membrane by insulin."
    Inoue M., Chang L., Hwang J., Chiang S.-H., Saltiel A.R.
    Nature 422:629-633(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARHQ.

Entry informationi

Entry nameiEXOC7_MOUSE
AccessioniPrimary (citable) accession number: O35250
Secondary accession number(s): Q8K121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: December 6, 2002
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3