Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Exocyst complex component 7

Gene

Exoc7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-264876. Insulin processing.
R-MMU-5620916. VxPx cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Exocyst complex component 7
Alternative name(s):
Exocyst complex component Exo70
Gene namesi
Name:Exoc7
Synonyms:Exo70
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1859270. Exoc7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001189611 – 697Exocyst complex component 7Add BLAST697

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei133PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35250.
PeptideAtlasiO35250.
PRIDEiO35250.

PTM databases

iPTMnetiO35250.
PhosphoSitePlusiO35250.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020792.
CleanExiMM_EXOC7.
ExpressionAtlasiO35250. baseline and differential.
GenevisibleiO35250. MM.

Interactioni

Subunit structurei

The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with RAB11FIP3 (By similarity). Interacts with ARHQ in a GTP-dependent manner.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Exoc4O353822EBI-775332,EBI-772648

Protein-protein interaction databases

IntActiO35250. 8 interactors.
MINTiMINT-1897779.
STRINGi10090.ENSMUSP00000021147.

Structurei

Secondary structure

1697
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi89 – 91Combined sources3
Helixi93 – 103Combined sources11
Helixi109 – 129Combined sources21
Helixi134 – 161Combined sources28
Helixi167 – 176Combined sources10
Helixi193 – 209Combined sources17
Helixi214 – 240Combined sources27
Helixi307 – 334Combined sources28
Helixi337 – 339Combined sources3
Helixi340 – 371Combined sources32
Turni377 – 380Combined sources4
Helixi381 – 398Combined sources18
Turni399 – 401Combined sources3
Helixi404 – 407Combined sources4
Helixi409 – 434Combined sources26
Helixi439 – 441Combined sources3
Helixi450 – 464Combined sources15
Helixi466 – 474Combined sources9
Helixi500 – 526Combined sources27
Helixi532 – 547Combined sources16
Turni548 – 552Combined sources5
Helixi555 – 560Combined sources6
Helixi566 – 582Combined sources17
Helixi583 – 585Combined sources3
Helixi586 – 589Combined sources4
Turni590 – 592Combined sources3
Helixi594 – 596Combined sources3
Helixi608 – 632Combined sources25
Helixi640 – 666Combined sources27
Helixi674 – 677Combined sources4
Helixi682 – 690Combined sources9
Beta strandi692 – 694Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PFTX-ray2.25A85-697[»]
ProteinModelPortaliO35250.
SMRiO35250.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35250.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 384SEC8 and ARHQ bindingAdd BLAST384

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili5 – 42Sequence analysisAdd BLAST38
Coiled coili63 – 85Sequence analysisAdd BLAST23

Domaini

The C-terminus is required for translocation to the plasma membrane.

Sequence similaritiesi

Belongs to the EXO70 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2344. Eukaryota.
ENOG410YIGI. LUCA.
GeneTreeiENSGT00390000003595.
HOGENOMiHOG000231194.
HOVERGENiHBG051515.
InParanoidiO35250.
KOiK07195.
OMAiPGPWINA.
OrthoDBiEOG091G02QC.
PhylomeDBiO35250.
TreeFamiTF324243.

Family and domain databases

InterProiIPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view]
PANTHERiPTHR12542. PTHR12542. 2 hits.
PfamiPF03081. Exo70. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35250-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIPPQEASAR RREIEDKLKQ EEETLSFIRD SLEKSDQLTR NMVSILSSFE
60 70 80 90 100
SRLMKLENSI IPVHKQTENL QRLQENVEKT LSCLDHVISY YHVASDTEKI
110 120 130 140 150
IREGPTGRLE EYLGSMAKIQ KAVEYFQDNS PDSPELNKVK LLFERGKESL
160 170 180 190 200
ESEFRSLMTR HSKVVSPVLL LDLISADDEL EVQEDVVLEH LPESVLRDVV
210 220 230 240 250
RISRWLVEYG RNQDFMNVYY QIRSSQLDRS IKGLKEHFRK SSSSSGVPYS
260 270 280 290 300
PAIPNKRKDT PTKKPIKRPG TIRKAQNLLK QYSQHGLDGK KGGSNLIPLE
310 320 330 340 350
GRDDMLDVET DAYIHCVSAF VKLAQSEYRL LMEIIPEHHQ KKTFDSLIQD
360 370 380 390 400
ALDGLMLEGE NIVSAARKAI IRHDFSTVLT VFPILRHLKQ TKPEFDQVLQ
410 420 430 440 450
GTAASTKNKL PGLITSMETI GAKALEDFAD NIKNDPDKEY NMPKDGTVHE
460 470 480 490 500
LTSNAILFLQ QLLDFQETAG AMLASQVLGD TYNIPLDPRE TSSSATSYSS
510 520 530 540 550
EFSKRLLSTY ICKVLGNLQL NLLSKSKVYE DPALSAIFLH NNYNYILKSL
560 570 580 590 600
EKSELIQLVA VTQKTAERSY REHIEQQIQT YQRSWLKVTD YIAEKNLPVF
610 620 630 640 650
QPGVKLRDKE RQMIKERFKG FNDGLEELCK IQKVWAIPDT EQRDKIRQAQ
660 670 680 690
KDIVKETYGA FLHRYGSVPF TKNPEKYIKY RVEQVGDMID RLFDTSA
Length:697
Mass (Da):79,960
Last modified:December 6, 2002 - v2
Checksum:i0A78F00E1D5D575A
GO
Isoform 2 (identifier: O35250-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-300: Missing.
     477-489: Missing.

Show »
Length:653
Mass (Da):75,159
Checksum:i40BB7E7298E82D53
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40R → K in AAB69345 (Ref. 1) Curated1
Sequence conflicti165V → I in AAB69345 (Ref. 1) Curated1
Sequence conflicti433K → E in AAB69345 (Ref. 1) Curated1
Sequence conflicti529Y → F in AAB69345 (Ref. 1) Curated1
Sequence conflicti610 – 611ER → DP in AAB69345 (Ref. 1) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001484270 – 300Missing in isoform 2. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_001485477 – 489Missing in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014461 mRNA. Translation: AAB69345.1.
BC028927 mRNA. Translation: AAH28927.1.
CCDSiCCDS25664.1. [O35250-1]
CCDS48983.1. [O35250-2]
PIRiT03722.
RefSeqiNP_001156344.1. NM_001162872.1. [O35250-2]
NP_058553.2. NM_016857.2. [O35250-1]
UniGeneiMm.22530.

Genome annotation databases

EnsembliENSMUST00000021147; ENSMUSP00000021147; ENSMUSG00000020792. [O35250-1]
ENSMUST00000106411; ENSMUSP00000102019; ENSMUSG00000020792. [O35250-2]
GeneIDi53413.
KEGGimmu:53413.
UCSCiuc007mks.2. mouse. [O35250-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014461 mRNA. Translation: AAB69345.1.
BC028927 mRNA. Translation: AAH28927.1.
CCDSiCCDS25664.1. [O35250-1]
CCDS48983.1. [O35250-2]
PIRiT03722.
RefSeqiNP_001156344.1. NM_001162872.1. [O35250-2]
NP_058553.2. NM_016857.2. [O35250-1]
UniGeneiMm.22530.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PFTX-ray2.25A85-697[»]
ProteinModelPortaliO35250.
SMRiO35250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35250. 8 interactors.
MINTiMINT-1897779.
STRINGi10090.ENSMUSP00000021147.

PTM databases

iPTMnetiO35250.
PhosphoSitePlusiO35250.

Proteomic databases

PaxDbiO35250.
PeptideAtlasiO35250.
PRIDEiO35250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021147; ENSMUSP00000021147; ENSMUSG00000020792. [O35250-1]
ENSMUST00000106411; ENSMUSP00000102019; ENSMUSG00000020792. [O35250-2]
GeneIDi53413.
KEGGimmu:53413.
UCSCiuc007mks.2. mouse. [O35250-1]

Organism-specific databases

CTDi23265.
MGIiMGI:1859270. Exoc7.

Phylogenomic databases

eggNOGiKOG2344. Eukaryota.
ENOG410YIGI. LUCA.
GeneTreeiENSGT00390000003595.
HOGENOMiHOG000231194.
HOVERGENiHBG051515.
InParanoidiO35250.
KOiK07195.
OMAiPGPWINA.
OrthoDBiEOG091G02QC.
PhylomeDBiO35250.
TreeFamiTF324243.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-264876. Insulin processing.
R-MMU-5620916. VxPx cargo-targeting to cilium.

Miscellaneous databases

ChiTaRSiExoc7. mouse.
EvolutionaryTraceiO35250.
PROiO35250.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020792.
CleanExiMM_EXOC7.
ExpressionAtlasiO35250. baseline and differential.
GenevisibleiO35250. MM.

Family and domain databases

InterProiIPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view]
PANTHERiPTHR12542. PTHR12542. 2 hits.
PfamiPF03081. Exo70. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiEXOC7_MOUSE
AccessioniPrimary (citable) accession number: O35250
Secondary accession number(s): Q8K121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: December 6, 2002
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.