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O35250

- EXOC7_MOUSE

UniProt

O35250 - EXOC7_MOUSE

Protein

Exocyst complex component 7

Gene

Exoc7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (06 Dec 2002)
      Previous versions | rss
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    Functioni

    Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. exocytosis Source: UniProtKB-KW
    2. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_207837. Insulin processing.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exocyst complex component 7
    Alternative name(s):
    Exocyst complex component Exo70
    Gene namesi
    Name:Exoc7
    Synonyms:Exo70
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1859270. Exoc7.

    Subcellular locationi

    Cytoplasmcytosol. Cell membrane; Peripheral membrane protein
    Note: Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.

    GO - Cellular componenti

    1. centriolar satellite Source: Ensembl
    2. cytosol Source: UniProtKB-SubCell
    3. exocyst Source: InterPro
    4. growth cone membrane Source: MGI
    5. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 697697Exocyst complex component 7PRO_0000118961Add
    BLAST

    Proteomic databases

    MaxQBiO35250.
    PaxDbiO35250.
    PRIDEiO35250.

    PTM databases

    PhosphoSiteiO35250.

    Expressioni

    Gene expression databases

    ArrayExpressiO35250.
    BgeeiO35250.
    CleanExiMM_EXOC7.
    GenevestigatoriO35250.

    Interactioni

    Subunit structurei

    The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with RAB11FIP3 By similarity. Interacts with ARHQ in a GTP-dependent manner.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Exoc4O353822EBI-775332,EBI-772648

    Protein-protein interaction databases

    IntActiO35250. 7 interactions.
    MINTiMINT-1897779.

    Structurei

    Secondary structure

    1
    697
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi89 – 913
    Helixi93 – 10311
    Helixi109 – 12921
    Helixi134 – 16128
    Helixi167 – 17610
    Helixi193 – 20917
    Helixi214 – 24027
    Helixi307 – 33428
    Helixi337 – 3393
    Helixi340 – 37132
    Turni377 – 3804
    Helixi381 – 39818
    Turni399 – 4013
    Helixi404 – 4074
    Helixi409 – 43426
    Helixi439 – 4413
    Helixi450 – 46415
    Helixi466 – 4749
    Helixi500 – 52627
    Helixi532 – 54716
    Turni548 – 5525
    Helixi555 – 5606
    Helixi566 – 58217
    Helixi583 – 5853
    Helixi586 – 5894
    Turni590 – 5923
    Helixi594 – 5963
    Helixi608 – 63225
    Helixi640 – 66627
    Helixi674 – 6774
    Helixi682 – 6909
    Beta strandi692 – 6943

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2PFTX-ray2.25A85-697[»]
    ProteinModelPortaliO35250.
    SMRiO35250. Positions 85-241, 306-696.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO35250.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 384384SEC8 and ARHQ bindingAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili5 – 4238Sequence AnalysisAdd
    BLAST
    Coiled coili63 – 8523Sequence AnalysisAdd
    BLAST

    Domaini

    The C-terminus is required for translocation to the plasma membrane.

    Sequence similaritiesi

    Belongs to the EXO70 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG245075.
    HOGENOMiHOG000231194.
    HOVERGENiHBG051515.
    InParanoidiO35250.
    KOiK07195.
    OMAiADIIPEH.
    OrthoDBiEOG7S4X64.
    PhylomeDBiO35250.
    TreeFamiTF324243.

    Family and domain databases

    InterProiIPR016159. Cullin_repeat-like_dom.
    IPR004140. Exo70.
    [Graphical view]
    PANTHERiPTHR12542. PTHR12542. 1 hit.
    PfamiPF03081. Exo70. 1 hit.
    [Graphical view]
    SUPFAMiSSF74788. SSF74788. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O35250-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIPPQEASAR RREIEDKLKQ EEETLSFIRD SLEKSDQLTR NMVSILSSFE    50
    SRLMKLENSI IPVHKQTENL QRLQENVEKT LSCLDHVISY YHVASDTEKI 100
    IREGPTGRLE EYLGSMAKIQ KAVEYFQDNS PDSPELNKVK LLFERGKESL 150
    ESEFRSLMTR HSKVVSPVLL LDLISADDEL EVQEDVVLEH LPESVLRDVV 200
    RISRWLVEYG RNQDFMNVYY QIRSSQLDRS IKGLKEHFRK SSSSSGVPYS 250
    PAIPNKRKDT PTKKPIKRPG TIRKAQNLLK QYSQHGLDGK KGGSNLIPLE 300
    GRDDMLDVET DAYIHCVSAF VKLAQSEYRL LMEIIPEHHQ KKTFDSLIQD 350
    ALDGLMLEGE NIVSAARKAI IRHDFSTVLT VFPILRHLKQ TKPEFDQVLQ 400
    GTAASTKNKL PGLITSMETI GAKALEDFAD NIKNDPDKEY NMPKDGTVHE 450
    LTSNAILFLQ QLLDFQETAG AMLASQVLGD TYNIPLDPRE TSSSATSYSS 500
    EFSKRLLSTY ICKVLGNLQL NLLSKSKVYE DPALSAIFLH NNYNYILKSL 550
    EKSELIQLVA VTQKTAERSY REHIEQQIQT YQRSWLKVTD YIAEKNLPVF 600
    QPGVKLRDKE RQMIKERFKG FNDGLEELCK IQKVWAIPDT EQRDKIRQAQ 650
    KDIVKETYGA FLHRYGSVPF TKNPEKYIKY RVEQVGDMID RLFDTSA 697
    Length:697
    Mass (Da):79,960
    Last modified:December 6, 2002 - v2
    Checksum:i0A78F00E1D5D575A
    GO
    Isoform 2 (identifier: O35250-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         270-300: Missing.
         477-489: Missing.

    Show »
    Length:653
    Mass (Da):75,159
    Checksum:i40BB7E7298E82D53
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 401R → K in AAB69345. 1 PublicationCurated
    Sequence conflicti165 – 1651V → I in AAB69345. 1 PublicationCurated
    Sequence conflicti433 – 4331K → E in AAB69345. 1 PublicationCurated
    Sequence conflicti529 – 5291Y → F in AAB69345. 1 PublicationCurated
    Sequence conflicti610 – 6112ER → DP in AAB69345. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei270 – 30031Missing in isoform 2. 1 PublicationVSP_001484Add
    BLAST
    Alternative sequencei477 – 48913Missing in isoform 2. 1 PublicationVSP_001485Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014461 mRNA. Translation: AAB69345.1.
    BC028927 mRNA. Translation: AAH28927.1.
    CCDSiCCDS25664.1. [O35250-1]
    CCDS48983.1. [O35250-2]
    PIRiT03722.
    RefSeqiNP_001156344.1. NM_001162872.1. [O35250-2]
    NP_058553.2. NM_016857.2. [O35250-1]
    UniGeneiMm.22530.

    Genome annotation databases

    EnsembliENSMUST00000021147; ENSMUSP00000021147; ENSMUSG00000020792. [O35250-1]
    ENSMUST00000106411; ENSMUSP00000102019; ENSMUSG00000020792. [O35250-2]
    GeneIDi53413.
    KEGGimmu:53413.
    UCSCiuc007mks.2. mouse. [O35250-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014461 mRNA. Translation: AAB69345.1 .
    BC028927 mRNA. Translation: AAH28927.1 .
    CCDSi CCDS25664.1. [O35250-1 ]
    CCDS48983.1. [O35250-2 ]
    PIRi T03722.
    RefSeqi NP_001156344.1. NM_001162872.1. [O35250-2 ]
    NP_058553.2. NM_016857.2. [O35250-1 ]
    UniGenei Mm.22530.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2PFT X-ray 2.25 A 85-697 [» ]
    ProteinModelPortali O35250.
    SMRi O35250. Positions 85-241, 306-696.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35250. 7 interactions.
    MINTi MINT-1897779.

    PTM databases

    PhosphoSitei O35250.

    Proteomic databases

    MaxQBi O35250.
    PaxDbi O35250.
    PRIDEi O35250.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021147 ; ENSMUSP00000021147 ; ENSMUSG00000020792 . [O35250-1 ]
    ENSMUST00000106411 ; ENSMUSP00000102019 ; ENSMUSG00000020792 . [O35250-2 ]
    GeneIDi 53413.
    KEGGi mmu:53413.
    UCSCi uc007mks.2. mouse. [O35250-1 ]

    Organism-specific databases

    CTDi 23265.
    MGIi MGI:1859270. Exoc7.

    Phylogenomic databases

    eggNOGi NOG245075.
    HOGENOMi HOG000231194.
    HOVERGENi HBG051515.
    InParanoidi O35250.
    KOi K07195.
    OMAi ADIIPEH.
    OrthoDBi EOG7S4X64.
    PhylomeDBi O35250.
    TreeFami TF324243.

    Enzyme and pathway databases

    Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_207837. Insulin processing.

    Miscellaneous databases

    ChiTaRSi EXOC7. mouse.
    EvolutionaryTracei O35250.
    NextBioi 310225.
    PROi O35250.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35250.
    Bgeei O35250.
    CleanExi MM_EXOC7.
    Genevestigatori O35250.

    Family and domain databases

    InterProi IPR016159. Cullin_repeat-like_dom.
    IPR004140. Exo70.
    [Graphical view ]
    PANTHERi PTHR12542. PTHR12542. 1 hit.
    Pfami PF03081. Exo70. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74788. SSF74788. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Guo W., Roth D., De Camilli P., Novick P.
      Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: FVB/N.
      Tissue: Colon.
    3. "The exocyst complex is required for targeting of Glut4 to the plasma membrane by insulin."
      Inoue M., Chang L., Hwang J., Chiang S.-H., Saltiel A.R.
      Nature 422:629-633(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ARHQ.

    Entry informationi

    Entry nameiEXOC7_MOUSE
    AccessioniPrimary (citable) accession number: O35250
    Secondary accession number(s): Q8K121
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2002
    Last sequence update: December 6, 2002
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3