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O35250 (EXOC7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exocyst complex component 7
Alternative name(s):
Exocyst complex component Exo70
Gene names
Name:Exoc7
Synonyms:Exo70
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length697 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion. Ref.3

Subunit structure

The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with RAB11FIP3 By similarity. Interacts with ARHQ in a GTP-dependent manner. Ref.3

Subcellular location

Cytoplasmcytosol. Cell membrane; Peripheral membrane protein. Note: Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.

Domain

The C-terminus is required for translocation to the plasma membrane.

Sequence similarities

Belongs to the EXO70 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Exoc4O353822EBI-775332,EBI-772648

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35250-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35250-2)

The sequence of this isoform differs from the canonical sequence as follows:
     270-300: Missing.
     477-489: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 697697Exocyst complex component 7
PRO_0000118961

Regions

Region1 – 384384SEC8 and ARHQ binding
Coiled coil5 – 4238 Potential
Coiled coil63 – 8523 Potential

Natural variations

Alternative sequence270 – 30031Missing in isoform 2.
VSP_001484
Alternative sequence477 – 48913Missing in isoform 2.
VSP_001485

Experimental info

Sequence conflict401R → K in AAB69345. Ref.1
Sequence conflict1651V → I in AAB69345. Ref.1
Sequence conflict4331K → E in AAB69345. Ref.1
Sequence conflict5291Y → F in AAB69345. Ref.1
Sequence conflict610 – 6112ER → DP in AAB69345. Ref.1

Secondary structure

.......................................................... 697
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2002. Version 2.
Checksum: 0A78F00E1D5D575A

FASTA69779,960
        10         20         30         40         50         60 
MIPPQEASAR RREIEDKLKQ EEETLSFIRD SLEKSDQLTR NMVSILSSFE SRLMKLENSI 

        70         80         90        100        110        120 
IPVHKQTENL QRLQENVEKT LSCLDHVISY YHVASDTEKI IREGPTGRLE EYLGSMAKIQ 

       130        140        150        160        170        180 
KAVEYFQDNS PDSPELNKVK LLFERGKESL ESEFRSLMTR HSKVVSPVLL LDLISADDEL 

       190        200        210        220        230        240 
EVQEDVVLEH LPESVLRDVV RISRWLVEYG RNQDFMNVYY QIRSSQLDRS IKGLKEHFRK 

       250        260        270        280        290        300 
SSSSSGVPYS PAIPNKRKDT PTKKPIKRPG TIRKAQNLLK QYSQHGLDGK KGGSNLIPLE 

       310        320        330        340        350        360 
GRDDMLDVET DAYIHCVSAF VKLAQSEYRL LMEIIPEHHQ KKTFDSLIQD ALDGLMLEGE 

       370        380        390        400        410        420 
NIVSAARKAI IRHDFSTVLT VFPILRHLKQ TKPEFDQVLQ GTAASTKNKL PGLITSMETI 

       430        440        450        460        470        480 
GAKALEDFAD NIKNDPDKEY NMPKDGTVHE LTSNAILFLQ QLLDFQETAG AMLASQVLGD 

       490        500        510        520        530        540 
TYNIPLDPRE TSSSATSYSS EFSKRLLSTY ICKVLGNLQL NLLSKSKVYE DPALSAIFLH 

       550        560        570        580        590        600 
NNYNYILKSL EKSELIQLVA VTQKTAERSY REHIEQQIQT YQRSWLKVTD YIAEKNLPVF 

       610        620        630        640        650        660 
QPGVKLRDKE RQMIKERFKG FNDGLEELCK IQKVWAIPDT EQRDKIRQAQ KDIVKETYGA 

       670        680        690 
FLHRYGSVPF TKNPEKYIKY RVEQVGDMID RLFDTSA 

« Hide

Isoform 2 [UniParc].

Checksum: 40BB7E7298E82D53
Show »

FASTA65375,159

References

« Hide 'large scale' references
[1]Guo W., Roth D., De Camilli P., Novick P.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: FVB/N.
Tissue: Colon.
[3]"The exocyst complex is required for targeting of Glut4 to the plasma membrane by insulin."
Inoue M., Chang L., Hwang J., Chiang S.-H., Saltiel A.R.
Nature 422:629-633(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ARHQ.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF014461 mRNA. Translation: AAB69345.1.
BC028927 mRNA. Translation: AAH28927.1.
PIRT03722.
RefSeqNP_001156344.1. NM_001162872.1.
NP_058553.2. NM_016857.2.
UniGeneMm.22530.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2PFTX-ray2.25A85-697[»]
ProteinModelPortalO35250.
SMRO35250. Positions 85-696.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35250. 7 interactions.
MINTMINT-1897779.

PTM databases

PhosphoSiteO35250.

Proteomic databases

PaxDbO35250.
PRIDEO35250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021147; ENSMUSP00000021147; ENSMUSG00000020792. [O35250-1]
ENSMUST00000106411; ENSMUSP00000102019; ENSMUSG00000020792. [O35250-2]
GeneID53413.
KEGGmmu:53413.
UCSCuc007mks.2. mouse. [O35250-1]

Organism-specific databases

CTD23265.
MGIMGI:1859270. Exoc7.

Phylogenomic databases

eggNOGNOG245075.
HOGENOMHOG000231194.
HOVERGENHBG051515.
InParanoidO35250.
KOK07195.
OMAADIIPEH.
OrthoDBEOG7S4X64.
PhylomeDBO35250.
TreeFamTF324243.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.

Gene expression databases

ArrayExpressO35250.
BgeeO35250.
CleanExMM_EXOC7.
GenevestigatorO35250.

Family and domain databases

InterProIPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view]
PANTHERPTHR12542. PTHR12542. 1 hit.
PfamPF03081. Exo70. 1 hit.
[Graphical view]
SUPFAMSSF74788. SSF74788. 2 hits.
ProtoNetSearch...

Other

ChiTaRSEXOC7. mouse.
EvolutionaryTraceO35250.
NextBio310225.
PROO35250.
SOURCESearch...

Entry information

Entry nameEXOC7_MOUSE
AccessionPrimary (citable) accession number: O35250
Secondary accession number(s): Q8K121
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: December 6, 2002
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot