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Reviewed, UniProtKB/Swiss-Prot O35245 (PKD2_MOUSE)

Last modified June 16, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Polycystin-2
Alternative name(s):
    Polycystic kidney disease 2 protein homolog
Gene names
Name: Pkd2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length966 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Functions as a calcium permeable cation channel. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis By similarity.

Subunit structure

Forms homooligomers. Interacts with PKD1. PKD1 requires the presence of PKD2 for stable expression. Interacts with CD2AP By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Expressed in mesenchymally derived structures in the developing embryo at day 12.5. In adult, mostly expressed in kidney. Ref.2

Domain

The C-terminal coiled-coil domain binds calcium and undergoes a calcium-induced conformation change. It is implicated in oligomerization and the interaction with PKD1 By similarity.

Sequence similarities

Belongs to the polycystin family.

Contains 1 EF-hand domain.

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Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentMembrane
   DomainCoiled coil
Transmembrane
   LigandCalcium
   Molecular functionIonic channel
   PTMGlycoprotein
Phosphoprotein
Gene Ontology (GO)
   Biological processJAK-STAT cascade

Inferred from direct assay. Source: MGI

cell cycle arrest

Inferred from direct assay. Source: MGI

detection of mechanical stimulus

Inferred from mutant phenotype. Source: MGI

determination of left/right symmetry

Inferred from mutant phenotype. Source: MGI

embryonic placenta development

Inferred from mutant phenotype. Source: MGI

heart development

Inferred from mutant phenotype. Source: MGI

kidney development

Inferred from mutant phenotype. Source: MGI

negative regulation of ryanodine-sensitive calcium-release channel activity

Inferred from direct assay. Source: MGI

regulation of cAMP metabolic process

Inferred from mutant phenotype. Source: MGI

release of sequestered calcium ion into cytosol

Inferred from mutant phenotype. Source: MGI

   Cellular componentendoplasmic reticulum

Inferred from direct assay. Source: MGI

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

motile primary cilium

Inferred from direct assay. Source: MGI

nonmotile primary cilium

Inferred from direct assay. Source: MGI

plasma membrane

Inferred from direct assay. Source: MGI

polycystin complex

Inferred from direct assay. Source: MGI

   Molecular functionATPase binding

Inferred from physical interaction. Source: MGI

calcium channel activity

Inferred from direct assay. Source: MGI

calcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

potassium channel activity

Inferred from direct assay. Source: MGI

receptor binding

Inferred from physical interaction. Source: MGI

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 966966Polycystin-2
PRO_0000164357

Regions

Topological domain1 – 221221Cytoplasmic Potential
Transmembrane222 – 24221 Potential
Topological domain243 – 466224Extracellular Potential
Transmembrane467 – 48721 Potential
Topological domain488 – 50316Cytoplasmic Potential
Transmembrane504 – 52421 Potential
Topological domain525 – 54824Extracellular Potential
Transmembrane549 – 56921 Potential
Topological domain570 – 59627Cytoplasmic Potential
Transmembrane597 – 61721 Potential
Topological domain618 – 65639Extracellular Potential
Transmembrane657 – 67721 Potential
Topological domain678 – 966289Cytoplasmic Potential
Domain748 – 78336EF-hand
Calcium binding761 – 77212 By similarity
Region702 – 79291EF-hand domain By similarity
Region801 – 82020Linker By similarity
Region821 – 966146C-terminal coiled coil domain By similarity
Coiled coil837 – 91781 Potential
Motif314 – 32613Polycystin motif
Compositional bias95 – 995Poly-Asp
Compositional bias151 – 1544Poly-Arg

Amino acid modifications

Modified residue1641Phosphoserine By similarity
Modified residue2521Phosphoserine By similarity
Modified residue2581Phosphothreonine By similarity
Modified residue2611Phosphoserine By similarity
Modified residue8501Phosphoserine By similarity
Modified residue8561Phosphoserine By similarity
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation3261N-linked (GlcNAc...) Potential
Glycosylation3601N-linked (GlcNAc...) Potential
Glycosylation3731N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3651I → M in CAA73727. Ref.2
Sequence conflict3651I → M in CAA74551. Ref.2
Sequence conflict3651I → M in CAA74552. Ref.2
Sequence conflict3701R → K in CAA73727. Ref.2
Sequence conflict3701R → K in CAA74551. Ref.2
Sequence conflict3701R → K in CAA74552. Ref.2
Sequence conflict5601A → S in CAA73727. Ref.2
Sequence conflict5601A → S in CAA74551. Ref.2
Sequence conflict688 – 6892DL → SV in CAA74551. Ref.2
Sequence conflict7461K → E in CAA73727. Ref.2
Sequence conflict7461K → E in CAA74551. Ref.2
Sequence conflict9421S → P in CAA73727. Ref.2
Sequence conflict9421S → P in CAA74551. Ref.2
Sequence conflict9571S → G in CAA73727. Ref.2
Sequence conflict9571S → G in CAA74551. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
O35245-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 88BDDDFC9049935F

FASTA966108,996
        10         20         30         40         50         60 
MVNSRRVQPQ PPGDAGRSPA PRASGPGRLV AGGAGLAVPG GLGEQRGLEI EMERIRQAAA 

        70         80         90        100        110        120 
RDPPAGASAS PSPPLSSCSR QAWSRDNPGF EAEEDDDDDE VEGEEGGMVV EMDVEWRPGS 

       130        140        150        160        170        180 
RRSASSSAVS SVGARGRGLG SYRGAAHLSG RRRRLEDQGA QCPSPAGGGD PLHRHLPLEG 

       190        200        210        220        230        240 
QPPRVAWAER LVRGLRGLWG TRLMEESNAN REKYLKSVLR ELVTYLFFLV VLCILTYGMM 

       250        260        270        280        290        300 
SSNVYYYTRT LSQLFIDTPV SKTEKTNFKT LSSMEDFWKF TEGSFLDGLY WKAQTSNHTQ 

       310        320        330        340        350        360 
ADNRSFIFYE NLLLGVPRLR QLRVRNGSCS IPQDLRDEIK ECYDVYSVSS EDRAPFGPRN 

       370        380        390        400        410        420 
GTAWIYTSER ELNGSSHWGI IASYSGAGYY LDLSRTREET AAQLAGLRRN FWLDRGTRAA 

       430        440        450        460        470        480 
FIDFSVYNAN INLFCVVRLL AEFPATGGVV PSWQFQPVKL IRYVTAFDFF LAACEIIFCF 

       490        500        510        520        530        540 
FIIYYVVEEI LEIRIHRLSY FRSFWNCLDV VIVVLSVVAM VINIYRMSNA EGLLQFLEDQ 

       550        560        570        580        590        600 
NSFPNFEHVA YWQIQFNNIA AVMVFLVWIK LFKFINFNRT MSQLSTTMSR CAKDLFGFTI 

       610        620        630        640        650        660 
MFSIIFLAYA QLAYLVFGTQ VDDFSTFQEC IFTQFRIILG DINFAEIEEA NRVLGPLYFT 

       670        680        690        700        710        720 
TFVFFMFFIL LNMFLAIIND SYSEVKSDLA QQKAEMELSD LIRKGCQKAL VKLKLKRNTV 

       730        740        750        760        770        780 
DAISESLRQG GGKLNFDELR QDLKGKGHTD AEIEAIFTKY DQDGDQELTE REHQQMRDDL 

       790        800        810        820        830        840 
EKEREDLDLE HSSLPRPMSS RSFPRSLDDS EEEDDEDSGH SSRRRGSISS GVSYEEFQVL 

       850        860        870        880        890        900 
VRRVDRMEHS IGSIVSKIDA VIVKLEIMER AKLKRREVLG RLLDGVAEDA RLGRDSEIHR 

       910        920        930        940        950        960 
EQMERLVREE LERWESDDAA SQTGHGVSTQ VGLGGQPHPR NSRPPSSQSA EGLEGGSGNG 


SANVHA

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References

[1]"Molecular cloning, cDNA sequence analysis, and chromosomal localization of mouse Pkd2."
Wu G.Q., Mochizuki T., Le T.C., Cai Y., Hayashi T., Reynolds D.M., Somlo S.
Genomics 45:220-223(1997) [PubMed: 9339380] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization of the murine polycystic kidney disease (Pkd2) gene."
Pennekamp P., Bogdanova N., Wilda M., Markoff A., Hameister H., Horst J., Dworniczak B.
Mamm. Genome 9:749-752(1998) [PubMed: 9716661] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF014010 mRNA. Translation: AAC53388.1.
Y13278 mRNA. Translation: CAA73727.1.
Y14105 expand/collapse EMBL AC list , Y14106, Y14107, Y14108, Y14109, Y14110, Y14111, Y14112, Y14113, Y14114, Y14115, Y14116, Y14117, Y14118, Y14119 Genomic DNA. Translation: CAA74551.1.
Y14120 mRNA. Translation: CAA74552.1.
IPIIPI00314352.
UniGeneMm.476406
Mm.6442

3D structure databases

HSSPHSSP built from PDB template 1CB1 based on UniProtKB P02632.
ModBaseSearch...

PTM databases

PhosphoSiteO35245.

Proteomic databases

PRIDEO35245.

Genome annotation databases

EnsemblENSMUSG00000034462. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:1099818. Pkd2.

Phylogenomic databases

HOGENOMO35245.
HOVERGENO35245.

Gene expression databases

ArrayExpressO35245.
BgeeO35245.
CleanExMM_PKD2.
GermOnlineENSMUSG00000034462. Mus musculus.

Family and domain databases

InterProIPR011992. EF-Hand_type.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR013122. PKD1_2_channel.
IPR003915. PKD_2.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PfamPF08016. PKD_channel. 1 hit.
[Graphical view]
PRINTSPR01433. POLYCYSTIN2.
PROSITEPS00018. EF_HAND_1. False negative.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry namePKD2_MOUSE
AccessionPrimary (citable) accession number: O35245
Secondary accession number(s): Q9QWP0, Q9Z193, Q9Z194
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents