Reviewed, UniProtKB/Swiss-Prot O35244 (PRDX6_RAT)

Names and origin

Protein names

Recommended name:
    Peroxiredoxin-6
    EC=1.11.1.15
Alternative name(s):
    Antioxidant protein 2
    1-Cys peroxiredoxin
      Short name=1-Cys PRX
    Acidic calcium-independent phospholipase A2
      Short name=aiPLA2
    EC=3.1.1.-
    Non-selenium glutathione peroxidase
      Short name=NSGPx
    EC=1.11.1.7
    Thiol-specific antioxidant protein

Gene names

Name:	Prdx6
Synonyms:	Aipla2, Aop2, Tsa

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in redox regulation of the cell. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. Donor + H2O2 = oxidized donor + 2 H2O.
Subunit structure	Homotetramer. May interact with HTR2A. Interacts with STH By similarity.
Subcellular location	Cytoplasm. Lysosome. Note: Also found in lung secretory organelles.
Miscellaneous	The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin By similarity. Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO3H) upon oxidative stress By similarity.
Sequence similarities	Belongs to the ahpC/TSA family. Rehydrin subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Lysosome
Domain	Redox-active center
Molecular function	Antioxidant Hydrolase Oxidoreductase Peroxidase
PTM	Disulfide bond Phosphoprotein
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro hydrogen peroxide catabolic process Inferred from direct assay. Source: RGD lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from direct assay. Source: RGD hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 224	223	Peroxiredoxin-6		PRO_0000135104
Regions
	Domain	5 – 169	165	Thioredoxin
Sites
	Active site	32	1	For phospholipase activity By similarity
	Active site	47	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Modified residue	44	1	Phosphothreonine By similarity
	Modified residue	89	1	Phosphotyrosine By similarity
	Disulfide bond	47		Interchain; in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35244-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: EE41D9079A708FD9	FASTA	224	24,819
	10 20 30 40 50 60 MPGGLLLGDE APNFEANTTI GHIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE 70 80 90 100 110 120 FAKRNVKLIA LSIDSVEDHF AWSKDINAYN GAAPTEKLPF PIIDDKDRDL AILLGMLDPA 130 140 150 160 170 180 EKDEKGMPVT ARVVFIFGPD KKLKLSILYP ATTGRNFDEI LRVVDSLQLT ASNPVATPVD 190 200 210 220 WKKGESVMVL PTLPEEEAKQ LFPKGVFTKE LPSGKKYLRY TPQP

References

[1]	Kim T.-S., Feinstein S.I., Dodia C., Hennigan B.B., Fisher A.B. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Lung.
[2]	Andreeva S. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Olfactory epithelium.
[3]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-41; 57-84; 98-118; 133-142; 156-162 AND 183-199, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
[4]	"Identification of a human cDNA clone for lysosomal type Ca2+-independent phospholipase A2 and properties of the expressed protein." Kim T.-S., Sundaresh C.S., Feinstein S.I., Dodia C., Skach W.R., Jain M.K., Nagase T., Seki N., Ishikawa K., Nomura N., Fisher A.B. J. Biol. Chem. 272:2542-2550(1997) [PubMed: 8999971] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-42 AND 146-163.
[5]	Erratum Kim T.-S., Sundaresh C.S., Feinstein S.I., Dodia C., Skach W.R., Jain M.K., Nagase T., Seki N., Ishikawa K., Nomura N., Fisher A.B. J. Biol. Chem. 272:10981-10981(1997)

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF014009 mRNA. Translation: AAB66341.1. Y17295 mRNA. Translation: CAA76732.1.
IPI	IPI00231260.
RefSeq	NP_446028.1.
UniGene	Rn.42
3D structure databases
HSSP	HSSP built from PDB template 1PRX based on UniProtKB P30041.
SMR	O35244. Positions 5-224.
ModBase	Search...
Protein family/group databases
PeroxiBase	4427. Rno1CysPrx.
PTM databases
PhosphoSite	O35244.
Proteomic databases
PRIDE	O35244.
Genome annotation databases
Ensembl	ENSRNOG00000002896. Rattus norvegicus. [Contig view]
GeneID	94167.
KEGG	rno:94167.
Organism-specific databases
RGD	71005. Prdx6.
Phylogenomic databases
HOVERGEN	O35244.
OMA	O35244. TARVVFI.
Enzyme and pathway databases
BRENDA	1.11.1.15. 248. 1.11.1.7. 248.
Gene expression databases
ArrayExpress	O35244.
GermOnline	ENSRNOG00000002896. Rattus norvegicus.
Family and domain databases
InterPro	IPR000866. Alkyl_hydroperoxide_Rdtase. IPR019479. Peroxiredoxin_C. IPR017936. Thioredoxin-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF10417. 1-cysPrx_C. 1 hit. PF00578. AhpC-TSA. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	617808.

Entry information

Entry name

PRDX6_RAT

Accession

Primary (citable) accession number: O35244

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)