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Protein

Protein FAN

Gene

Nsmaf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.

GO - Molecular functioni

  • death receptor binding Source: MGI
  • phospholipid binding Source: GO_Central
  • sphingomyelin phosphodiesterase activator activity Source: Ensembl

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-5626978. TNFR1-mediated ceramide production.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein FAN
Alternative name(s):
Factor associated with neutral sphingomyelinase activation
Short name:
Factor associated with N-SMase activation
Gene namesi
Name:Nsmaf
Synonyms:Fan
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1341864. Nsmaf.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Protein FANPRO_0000050976Add
BLAST

Proteomic databases

EPDiO35242.
MaxQBiO35242.
PaxDbiO35242.
PeptideAtlasiO35242.
PRIDEiO35242.

PTM databases

iPTMnetiO35242.
PhosphoSiteiO35242.

Expressioni

Gene expression databases

BgeeiO35242.
ExpressionAtlasiO35242. baseline and differential.
GenevisibleiO35242. MM.

Interactioni

GO - Molecular functioni

  • death receptor binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029910.

Structurei

3D structure databases

ProteinModelPortaliO35242.
SMRiO35242. Positions 200-919.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini176 – 24772GRAMAdd
BLAST
Domaini189 – 28698BEACH-type PHPROSITE-ProRule annotationAdd
BLAST
Domaini290 – 575286BEACHPROSITE-ProRule annotationAdd
BLAST
Repeati631 – 66131WD 1Add
BLAST
Repeati673 – 70331WD 2Add
BLAST
Repeati715 – 74329WD 3Add
BLAST
Repeati764 – 79431WD 4Add
BLAST
Repeati806 – 83631WD 5Add
BLAST
Repeati887 – 91731WD 6Add
BLAST

Sequence similaritiesi

Contains 1 BEACH domain.PROSITE-ProRule annotation
Contains 1 BEACH-type PH domain.PROSITE-ProRule annotation
Contains 1 GRAM domain.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1786. Eukaryota.
ENOG410XNQC. LUCA.
GeneTreeiENSGT00760000119083.
HOGENOMiHOG000231605.
HOVERGENiHBG005639.
InParanoidiO35242.
KOiK18953.
OMAiAPYKIER.
OrthoDBiEOG70CR68.
TreeFamiTF324912.

Family and domain databases

Gene3Di1.10.1540.10. 1 hit.
2.130.10.10. 1 hit.
2.30.29.40. 1 hit.
InterProiIPR000409. BEACH_dom.
IPR004182. GRAM.
IPR023362. PH-BEACH_dom.
IPR011993. PH_dom-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02138. Beach. 1 hit.
PF02893. GRAM. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM01026. Beach. 1 hit.
SM00568. GRAM. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF50978. SSF50978. 1 hit.
SSF81837. SSF81837. 1 hit.
PROSITEiPS50197. BEACH. 1 hit.
PS51783. PH_BEACH. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFTRKRQRE QQLQLYSKER FSLLLLNLEE YYFEQHTAFH VQHQGSQEER
60 70 80 90 100
KIRGSLKICS KSVIFEPDAI SQPILKIPLR DCLKIGKHGE NGANKHFAKA
110 120 130 140 150
KSWGISLIFS QIYFIKEHNI VAPYKIERGK MEYVFELEVS GKVEDVVETL
160 170 180 190 200
LQLHRASCLD KLGDQMAMIT AILQSRLART SFDKNRFQSV SEKLHMECKA
210 220 230 240 250
EMVTPLVTNP GHVCITDTSL YFQPLNGYPK PVVQITLQDV RRIYKRRHGL
260 270 280 290 300
MPLGLEVFCT DDDLCSDIYL KFYEPQDRDD LYFYIATYLE HHAAEHTAES
310 320 330 340 350
YMLQWQRGHL SNYQYLLHLN NLADRSCNDL SQYPVFPWII SDYSSPELDL
360 370 380 390 400
SNPATFRDLS KPVGALNAER LERLLTRYQE MPEPRFMYGS HYSSPGYVLF
410 420 430 440 450
YLVRIAPEYM LCLQNGRFDN ADRMFNSIAE TWKNCLDGAT DFKELIPEFY
460 470 480 490 500
DEDVSFLVNS LKLDLGKRQG GQMVDDVDLP AWASSPQDFL QKNKDALESG
510 520 530 540 550
YVSEHLHEWI DLIFGYKQKG SEAIGAHNVF HPLTYEGGVD LNSIEDPDEK
560 570 580 590 600
VAMLTQILEF GQTPKQLFVT PHPRRITPKF KSLSQASSYN ASLTDSPVSP
610 620 630 640 650
GEESFEDLTE ESRTLAWSNI AKLQLHEQYK IHKEAVTGIA VSCNGSSVFT
660 670 680 690 700
TSQDSTLKMF SKESKMLQRS ISFSNMALSS CLLLPGDTTV ISSSWDNNVY
710 720 730 740 750
FYSIAFGRRQ DTLMGHDDAV SKICWHNDRL YSGSWDSTVK VWSGVPAEMP
760 770 780 790 800
GTKRHQFDLL AELEHDVSVN TINLNAVSTL LVSGTKEGMV NIWDLTTATL
810 820 830 840 850
LHQTSCHSGT VCDAAFSPDS RHILSTGVDG CLNVIDVQTG MLISSMASEE
860 870 880 890 900
PQRCFVWDGN SVLSGSRSGE LLVWDLLGAK VSERIQGHTG AVTCMWMNEQ
910 920
CSSIITGGED RQIMFWKLQY
Length:920
Mass (Da):104,546
Last modified:June 28, 2011 - v2
Checksum:iBB50AD4AB77CE124
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti385 – 3862RF → KS in AAB65394 (PubMed:10228161).Curated
Sequence conflicti390 – 3901S → N in AAB65394 (PubMed:10228161).Curated
Sequence conflicti849 – 8491E → D in AAB65394 (PubMed:10228161).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013632 mRNA. Translation: AAB65394.1.
BX510318, AL772306 Genomic DNA. Translation: CAM20130.1.
AL772306, BX510318 Genomic DNA. Translation: CAM27232.1.
CH466538 Genomic DNA. Translation: EDL05686.1.
BC145961 mRNA. Translation: AAI45962.1.
BC145963 mRNA. Translation: AAI45964.1.
CCDSiCCDS17952.1.
RefSeqiNP_035075.2. NM_010945.2.
UniGeneiMm.3059.

Genome annotation databases

EnsembliENSMUST00000029910; ENSMUSP00000029910; ENSMUSG00000028245.
GeneIDi18201.
KEGGimmu:18201.
UCSCiuc008rxp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013632 mRNA. Translation: AAB65394.1.
BX510318, AL772306 Genomic DNA. Translation: CAM20130.1.
AL772306, BX510318 Genomic DNA. Translation: CAM27232.1.
CH466538 Genomic DNA. Translation: EDL05686.1.
BC145961 mRNA. Translation: AAI45962.1.
BC145963 mRNA. Translation: AAI45964.1.
CCDSiCCDS17952.1.
RefSeqiNP_035075.2. NM_010945.2.
UniGeneiMm.3059.

3D structure databases

ProteinModelPortaliO35242.
SMRiO35242. Positions 200-919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029910.

PTM databases

iPTMnetiO35242.
PhosphoSiteiO35242.

Proteomic databases

EPDiO35242.
MaxQBiO35242.
PaxDbiO35242.
PeptideAtlasiO35242.
PRIDEiO35242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029910; ENSMUSP00000029910; ENSMUSG00000028245.
GeneIDi18201.
KEGGimmu:18201.
UCSCiuc008rxp.2. mouse.

Organism-specific databases

CTDi8439.
MGIiMGI:1341864. Nsmaf.

Phylogenomic databases

eggNOGiKOG1786. Eukaryota.
ENOG410XNQC. LUCA.
GeneTreeiENSGT00760000119083.
HOGENOMiHOG000231605.
HOVERGENiHBG005639.
InParanoidiO35242.
KOiK18953.
OMAiAPYKIER.
OrthoDBiEOG70CR68.
TreeFamiTF324912.

Enzyme and pathway databases

ReactomeiR-MMU-5626978. TNFR1-mediated ceramide production.

Miscellaneous databases

ChiTaRSiNsmaf. mouse.
PROiO35242.
SOURCEiSearch...

Gene expression databases

BgeeiO35242.
ExpressionAtlasiO35242. baseline and differential.
GenevisibleiO35242. MM.

Family and domain databases

Gene3Di1.10.1540.10. 1 hit.
2.130.10.10. 1 hit.
2.30.29.40. 1 hit.
InterProiIPR000409. BEACH_dom.
IPR004182. GRAM.
IPR023362. PH-BEACH_dom.
IPR011993. PH_dom-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02138. Beach. 1 hit.
PF02893. GRAM. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM01026. Beach. 1 hit.
SM00568. GRAM. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF50978. SSF50978. 1 hit.
SSF81837. SSF81837. 1 hit.
PROSITEiPS50197. BEACH. 1 hit.
PS51783. PH_BEACH. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Impaired neutral sphingomyelinase activation and cutaneous barrier repair in FAN-deficient mice."
    Kreder D., Krut O., Adam-Klages S., Wiegmann K., Scherer G., Plitz T., Jensen J.M., Proksch E., Steinmann J., Pfeffer K., Kroenke M.
    EMBO J. 18:2472-2479(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiFAN_MOUSE
AccessioniPrimary (citable) accession number: O35242
Secondary accession number(s): A2AKK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 28, 2011
Last modified: July 6, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.