O35231 (KIFC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIFC3

Gene names

Name:

Kifc3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance. Ref.1
Subunit structure	Interacts with annexin XIIIB. Ref.1
Subcellular location	Cytoplasm › cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein Probable. Cell junction › adherens junction By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Note: Localizes along zonula adherens only at mature cell-cell contacts By similarity. Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells. Ref.1
Tissue specificity	Predominant expression in the kidney, testis and ovary. Also expressed in brain, heart, liver, lung and uterus. Ref.1
Sequence similarities	Belongs to the kinesin-like protein family. Contains 1 kinesin-motor domain.
Sequence caution	The sequence AAH70429.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE21409.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasm Cytoplasmic vesicle Cytoskeleton Membrane Microtubule
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi organization Inferred from mutant phenotype PubMed 12135985. Source: MGI epithelial cell-cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB microtubule-based movement Inferred from electronic annotation. Source: InterPro microtubule-based process Inferred from direct assay PubMed 12135985. Source: MGI zonula adherens maintenance Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 12135985. Source: MGI centrosome Inferred from sequence or structural similarity. Source: UniProtKB cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell kinesin complex Inferred from direct assay PubMed 12135985. Source: MGI microtubule Inferred from electronic annotation. Source: UniProtKB-KW zonula adherens Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW minus-end-directed microtubule motor activity Traceable author statement PubMed 12135985. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O35231-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O35231-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.
     116-125: QEVSRLRSEL → MPPLGPGSPQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 824	824	Kinesin-like protein KIFC3		PRO_0000125432
Regions
	Domain	440 – 697	258	Kinesin-motor
	Nucleotide binding	526 – 533	8	ATP By similarity
	Coiled coil	100 – 360	261	Potential
	Coiled coil	393 – 430	38	Potential
Natural variations
	Alternative sequence	1 – 115	115	Missing in isoform 2.		VSP_022363
	Alternative sequence	116 – 125	10	QEVSRLRSEL → MPPLGPGSPQ in isoform 2.		VSP_022364
Experimental info
	Sequence conflict	67 – 74	8	EDSTSRTA → HCQPTFPT in BAE21409. Ref.4
	Sequence conflict	76 – 83	8	RPSLAQCR → GLRAQHQP in BAE21409. Ref.4
	Sequence conflict	86 – 103	18	SVDWP…YLTVQ → ETAKVRTSALGVVRRCGE in BAE21409. Ref.4
	Sequence conflict	122	1	R → Q in BAE92567. Ref.1
	Sequence conflict	214 – 215	2	EL → DV in AAC39967. Ref.2
	Sequence conflict	237	1	D → Y in BAE92567. Ref.1
	Sequence conflict	573	1	A → R in AAC39967. Ref.2
	Sequence conflict	659	1	S → R in AAC39967. Ref.2
	Sequence conflict	690	1	R → Q in BAE92567. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: EE7EF66140725547	FASTA	824	92,556
	10 20 30 40 50 60 MVPSRRTWNL GATPSLRGLW RVGRVQEPKP GMARPAPASP AARPFPHTGQ GRLRTGRGKD 70 80 90 100 110 120 ILPSGEEDST SRTAARPSLA QCRALSVDWP GPRSPHRLYL TVQVENLKEK LISQAQEVSR 130 140 150 160 170 180 LRSELGGTDA EKHRDRLMVE NEQLRQELRR CEVELQELRA QPVVPCEGCE HSQESSQLRD 190 200 210 220 230 240 KLSQLQLEVA ENKGMLSELN LEVQQKTDRL AEVELRLKDC LAEKAQEEER LSRRLRDSHE 250 260 270 280 290 300 TIASLRAQSP PVKYVIKTVE VESSKTKQAL SESQTRNQHL QEQVAMQRQV LKEMEQQLQN 310 320 330 340 350 360 SHQLTVQLRA QIAMYEAELE RAHGQMLEEM QSLEEDKNRA IEEAFARAQV EMKAVHENLA 370 380 390 400 410 420 GVRTNLLTLQ PALRTLTNDY NGLKRQVRGF PLLLQEALRS VKAEIGQAIE EVNSNNQELL 430 440 450 460 470 480 RKYRRELQLR KKCHNELVRL KGNIRVIARV RPVTKEDGEG PEATNAVTFD PDDDSIIHLL 490 500 510 520 530 540 HKGKPVSFEL DKVFSPWASQ QDVFQEVQAL ITSCIDGFNV CIFAYGQTGA GKTYTMEGTP 550 560 570 580 590 600 ENPGINQRAL QLLFSEVQEK ASDWQYNITV SAAEIYNEVL RDLLGKEPQE KLEIRLCPDG 610 620 630 640 650 660 SGQLYVPGLT EFQVQSVDDI NKVFEFGYNN RTTEFTNLNE HSSRSHALLI VTVRGVDCST 670 680 690 700 710 720 GLRTTGKLNL VDLAGSERVG KSGAEGNRLR EAQHINRSLS ALGDVIAALR SRQGHVPFRN 730 740 750 760 770 780 SKLTYLLQDS LSGDSKTLMV VQVSPVEKNT SETLYSLRFA ERVRSVELGP GSRRTELGSW 790 800 810 820 SSQEHLEWEP ACQTPQPTAR AHSAPGSGTS SRPGSIRRKL QPSA
	Isoform 2 [UniParc]. Checksum: CF5CF42160DEADE4	FASTA	709	79,740
	10 20 30 40 50 60 MPPLGPGSPQ GGTDAEKHRD RLMVENEQLR QELRRCEVEL QELRAQPVVP CEGCEHSQES 70 80 90 100 110 120 SQLRDKLSQL QLEVAENKGM LSELNLEVQQ KTDRLAEVEL RLKDCLAEKA QEEERLSRRL 130 140 150 160 170 180 RDSHETIASL RAQSPPVKYV IKTVEVESSK TKQALSESQT RNQHLQEQVA MQRQVLKEME 190 200 210 220 230 240 QQLQNSHQLT VQLRAQIAMY EAELERAHGQ MLEEMQSLEE DKNRAIEEAF ARAQVEMKAV 250 260 270 280 290 300 HENLAGVRTN LLTLQPALRT LTNDYNGLKR QVRGFPLLLQ EALRSVKAEI GQAIEEVNSN 310 320 330 340 350 360 NQELLRKYRR ELQLRKKCHN ELVRLKGNIR VIARVRPVTK EDGEGPEATN AVTFDPDDDS 370 380 390 400 410 420 IIHLLHKGKP VSFELDKVFS PWASQQDVFQ EVQALITSCI DGFNVCIFAY GQTGAGKTYT 430 440 450 460 470 480 MEGTPENPGI NQRALQLLFS EVQEKASDWQ YNITVSAAEI YNEVLRDLLG KEPQEKLEIR 490 500 510 520 530 540 LCPDGSGQLY VPGLTEFQVQ SVDDINKVFE FGYNNRTTEF TNLNEHSSRS HALLIVTVRG 550 560 570 580 590 600 VDCSTGLRTT GKLNLVDLAG SERVGKSGAE GNRLREAQHI NRSLSALGDV IAALRSRQGH 610 620 630 640 650 660 VPFRNSKLTY LLQDSLSGDS KTLMVVQVSP VEKNTSETLY SLRFAERVRS VELGPGSRRT 670 680 690 700 ELGSWSSQEH LEWEPACQTP QPTARAHSAP GSGTSSRPGS IRRKLQPSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes." Noda Y., Okada Y., Saito N., Setou M., Xu Y., Zhang Z., Hirokawa N. J. Cell Biol. 155:77-88(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ANNEXIN XIIIB. Strain: ICR. Tissue: Brain.
[2]	"Molecular cloning and functional analysis of mouse C-terminal kinesin motor KifC3." Yang Z., Xia C.H., Roberts E.A., Bush K., Nigam S.K., Goldstein L.S. Mol. Cell. Biol. 21:765-770(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6, Czech II and FVB/N. Tissue: Brain and Mammary tumor.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 66-824 (ISOFORM 1). Strain: C57BL/6J. Tissue: Testis.
[5]	"Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome." Nakagawa T., Tanaka Y., Matsuoka E., Kondo S., Okada Y., Noda Y., Kanai Y., Hirokawa N. Proc. Natl. Acad. Sci. U.S.A. 94:9654-9659(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 522-678. Strain: ICR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49546 mRNA. Translation: BAE92567.1. AF013118 mRNA. Translation: AAC39967.2. BC004069 mRNA. Translation: AAH04069.1. BC016118 mRNA. Translation: AAH16118.1. BC023374 mRNA. Translation: AAH23374.1. BC070429 mRNA. Translation: AAH70429.1. Different initiation. AK132895 mRNA. Translation: BAE21409.1. Different initiation. AK135926 mRNA. Translation: BAE22727.1. AB001457 mRNA. Translation: BAA22399.1.
IPI	IPI00421009. IPI00828544.
RefSeq	NP_001139303.1. NM_001145831.1. NP_001139304.1. NM_001145832.1. NP_034761.3. NM_010631.2.
UniGene	Mm.378951.
3D structure databases
ProteinModelPortal	O35231.
SMR	O35231. Positions 443-764.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000034240.
PTM databases
PhosphoSite	O35231.
Proteomic databases
PaxDb	O35231.
PRIDE	O35231.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034240; ENSMUSP00000034240; ENSMUSG00000031788. ENSMUST00000169353; ENSMUSP00000127427; ENSMUSG00000031788.
GeneID	16582.
KEGG	mmu:16582.
UCSC	uc009mxu.1. mouse. uc009mxv.1. mouse.
Organism-specific databases
CTD	3801.
MGI	MGI:109202. Kifc3.
Phylogenomic databases
eggNOG	COG5059.
GeneTree	ENSGT00550000074610.
HOVERGEN	HBG052259.
InParanoid	O35231.
KO	K10406.
OMA	WRVGRAP.
OrthoDB	EOG4HHP28.
Enzyme and pathway databases
Reactome	REACT_93132. Metabolism of proteins.
Gene expression databases
ArrayExpress	O35231.
Bgee	O35231.
CleanEx	MM_KIFC3.
Genevestigator	O35231.
GermOnline	ENSMUSG00000031788. Mus musculus.
Family and domain databases
Gene3D	3.40.850.10. 1 hit.
InterPro	IPR027325. KIFC3. IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
PANTHER	PTHR24115:SF173. PTHR24115:SF173. 1 hit.
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	KIFC3. mouse.
NextBio	290125.
SOURCE	Search...

Entry information

Entry name

KIFC3_MOUSE

Accession

Primary (citable) accession number: O35231
Secondary accession number(s): O35072 , Q1WNZ8, Q3UX36, Q3V0U4, Q6NS71, Q8R3Y4, Q91YQ2, Q99KP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 89 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program