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O35231 (KIFC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein KIFC3
Gene names
Name:Kifc3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance. Ref.1

Subunit structure

Interacts with annexin XIIIB. Ref.1

Subcellular location

Cytoplasmcytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein Probable. Cell junctionadherens junction By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Note: Localizes along zonula adherens only at mature cell-cell contacts By similarity. Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells. Ref.1

Tissue specificity

Predominant expression in the kidney, testis and ovary. Also expressed in brain, heart, liver, lung and uterus. Ref.1

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

Contains 1 kinesin motor domain.

Sequence caution

The sequence AAH70429.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAE21409.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCell junction
Cytoplasm
Cytoplasmic vesicle
Cytoskeleton
Membrane
Microtubule
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionMotor protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi organization

Inferred from mutant phenotype PubMed 12135985. Source: MGI

epithelial cell-cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

metabolic process

Traceable author statement PubMed 12135985. Source: GOC

microtubule-based movement

Inferred from electronic annotation. Source: InterPro

microtubule-based process

Inferred from direct assay PubMed 12135985. Source: MGI

zonula adherens maintenance

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 12135985. Source: MGI

centrosome

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

kinesin complex

Inferred from direct assay PubMed 12135985. Source: MGI

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

zonula adherens

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

minus-end-directed microtubule motor activity

Traceable author statement PubMed 12135985. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35231-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35231-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.
     116-125: QEVSRLRSEL → MPPLGPGSPQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 824824Kinesin-like protein KIFC3
PRO_0000125432

Regions

Domain443 – 766324Kinesin motor
Nucleotide binding526 – 5338ATP By similarity
Coiled coil100 – 360261 Potential
Coiled coil393 – 43038 Potential

Natural variations

Alternative sequence1 – 115115Missing in isoform 2.
VSP_022363
Alternative sequence116 – 12510QEVSRLRSEL → MPPLGPGSPQ in isoform 2.
VSP_022364

Experimental info

Sequence conflict67 – 748EDSTSRTA → HCQPTFPT in BAE21409. Ref.4
Sequence conflict76 – 838RPSLAQCR → GLRAQHQP in BAE21409. Ref.4
Sequence conflict86 – 10318SVDWP…YLTVQ → ETAKVRTSALGVVRRCGE in BAE21409. Ref.4
Sequence conflict1221R → Q in BAE92567. Ref.1
Sequence conflict214 – 2152EL → DV in AAC39967. Ref.2
Sequence conflict2371D → Y in BAE92567. Ref.1
Sequence conflict5731A → R in AAC39967. Ref.2
Sequence conflict6591S → R in AAC39967. Ref.2
Sequence conflict6901R → Q in BAE92567. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: EE7EF66140725547

FASTA82492,556
        10         20         30         40         50         60 
MVPSRRTWNL GATPSLRGLW RVGRVQEPKP GMARPAPASP AARPFPHTGQ GRLRTGRGKD 

        70         80         90        100        110        120 
ILPSGEEDST SRTAARPSLA QCRALSVDWP GPRSPHRLYL TVQVENLKEK LISQAQEVSR 

       130        140        150        160        170        180 
LRSELGGTDA EKHRDRLMVE NEQLRQELRR CEVELQELRA QPVVPCEGCE HSQESSQLRD 

       190        200        210        220        230        240 
KLSQLQLEVA ENKGMLSELN LEVQQKTDRL AEVELRLKDC LAEKAQEEER LSRRLRDSHE 

       250        260        270        280        290        300 
TIASLRAQSP PVKYVIKTVE VESSKTKQAL SESQTRNQHL QEQVAMQRQV LKEMEQQLQN 

       310        320        330        340        350        360 
SHQLTVQLRA QIAMYEAELE RAHGQMLEEM QSLEEDKNRA IEEAFARAQV EMKAVHENLA 

       370        380        390        400        410        420 
GVRTNLLTLQ PALRTLTNDY NGLKRQVRGF PLLLQEALRS VKAEIGQAIE EVNSNNQELL 

       430        440        450        460        470        480 
RKYRRELQLR KKCHNELVRL KGNIRVIARV RPVTKEDGEG PEATNAVTFD PDDDSIIHLL 

       490        500        510        520        530        540 
HKGKPVSFEL DKVFSPWASQ QDVFQEVQAL ITSCIDGFNV CIFAYGQTGA GKTYTMEGTP 

       550        560        570        580        590        600 
ENPGINQRAL QLLFSEVQEK ASDWQYNITV SAAEIYNEVL RDLLGKEPQE KLEIRLCPDG 

       610        620        630        640        650        660 
SGQLYVPGLT EFQVQSVDDI NKVFEFGYNN RTTEFTNLNE HSSRSHALLI VTVRGVDCST 

       670        680        690        700        710        720 
GLRTTGKLNL VDLAGSERVG KSGAEGNRLR EAQHINRSLS ALGDVIAALR SRQGHVPFRN 

       730        740        750        760        770        780 
SKLTYLLQDS LSGDSKTLMV VQVSPVEKNT SETLYSLRFA ERVRSVELGP GSRRTELGSW 

       790        800        810        820 
SSQEHLEWEP ACQTPQPTAR AHSAPGSGTS SRPGSIRRKL QPSA 

« Hide

Isoform 2 [UniParc].

Checksum: CF5CF42160DEADE4
Show »

FASTA70979,740

References

« Hide 'large scale' references
[1]"KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes."
Noda Y., Okada Y., Saito N., Setou M., Xu Y., Zhang Z., Hirokawa N.
J. Cell Biol. 155:77-88(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ANNEXIN XIIIB.
Strain: ICR.
Tissue: Brain.
[2]"Molecular cloning and functional analysis of mouse C-terminal kinesin motor KifC3."
Yang Z., Xia C.H., Roberts E.A., Bush K., Nigam S.K., Goldstein L.S.
Mol. Cell. Biol. 21:765-770(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6, Czech II and FVB/N.
Tissue: Brain and Mammary tumor.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 66-824 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Testis.
[5]"Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome."
Nakagawa T., Tanaka Y., Matsuoka E., Kondo S., Okada Y., Noda Y., Kanai Y., Hirokawa N.
Proc. Natl. Acad. Sci. U.S.A. 94:9654-9659(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 522-678.
Strain: ICR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D49546 mRNA. Translation: BAE92567.1.
AF013118 mRNA. Translation: AAC39967.2.
BC004069 mRNA. Translation: AAH04069.1.
BC016118 mRNA. Translation: AAH16118.1.
BC023374 mRNA. Translation: AAH23374.1.
BC070429 mRNA. Translation: AAH70429.1. Different initiation.
AK132895 mRNA. Translation: BAE21409.1. Different initiation.
AK135926 mRNA. Translation: BAE22727.1.
AB001457 mRNA. Translation: BAA22399.1.
CCDSCCDS52640.1. [O35231-1]
RefSeqNP_001139303.1. NM_001145831.1.
NP_001139304.1. NM_001145832.1.
NP_034761.3. NM_010631.2. [O35231-1]
UniGeneMm.378951.

3D structure databases

ProteinModelPortalO35231.
SMRO35231. Positions 443-764.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000034240.

PTM databases

PhosphoSiteO35231.

Proteomic databases

PaxDbO35231.
PRIDEO35231.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034240; ENSMUSP00000034240; ENSMUSG00000031788. [O35231-1]
ENSMUST00000169353; ENSMUSP00000127427; ENSMUSG00000031788.
GeneID16582.
KEGGmmu:16582.
UCSCuc009mxu.1. mouse. [O35231-2]
uc009mxv.1. mouse. [O35231-1]

Organism-specific databases

CTD3801.
MGIMGI:109202. Kifc3.

Phylogenomic databases

eggNOGCOG5059.
GeneTreeENSGT00550000074610.
HOVERGENHBG052259.
InParanoidO35231.
KOK10406.
OMALTEFRVQ.
OrthoDBEOG72G16X.
PhylomeDBO35231.
TreeFamTF105238.

Gene expression databases

BgeeO35231.
CleanExMM_KIFC3.
GenevestigatorO35231.

Family and domain databases

Gene3D3.40.850.10. 1 hit.
InterProIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR24115. PTHR24115. 1 hit.
PfamPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKIFC3. mouse.
NextBio290125.
PROO35231.
SOURCESearch...

Entry information

Entry nameKIFC3_MOUSE
AccessionPrimary (citable) accession number: O35231
Secondary accession number(s): O35072 expand/collapse secondary AC list , Q1WNZ8, Q3UX36, Q3V0U4, Q6NS71, Q8R3Y4, Q91YQ2, Q99KP7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 101 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot