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O35228 (IL27B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-27 subunit beta

Short name=IL-27 subunit beta
Short name=IL-27B
Alternative name(s):
Epstein-Barr virus-induced gene 3 protein homolog
Gene names
Name:Ebi3
Synonyms:Il27b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.

Subunit structure

Heterodimer with IL27/IL27A; not disulfide-linked. This heterodimer is known as interleukin IL-27. EBI3 is also a component of the IL-12 heterodimer. Interacts with SQSTM1 By similarity. Heterodimer with IL12A; this heterodimer is known as interleukin IL-35 By similarity.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 3 subfamily.

Contains 2 fibronectin type-III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 228210Interleukin-27 subunit beta
PRO_0000010938

Regions

Domain26 – 12499Fibronectin type-III 1
Domain127 – 22498Fibronectin type-III 2

Amino acid modifications

Glycosylation541N-linked (GlcNAc...) Potential
Glycosylation1041N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O35228 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 49DEFA4E5C4F2126

FASTA22825,353
        10         20         30         40         50         60 
MSKLLFLSLA LWASRSPGYT ETALVALSQP RVQCHASRYP VAVDCSWTPL QAPNSTRSTS 

        70         80         90        100        110        120 
FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP YMLNVTAVHP GGASSSLLAF 

       130        140        150        160        170        180 
VAERIIKPDP PEGVRLRTAG QRLQVLWHPP ASWPFPDIFS LKYRLRYRRR GASHFRQVGP 

       190        200        210        220 
IEATTFTLRN SKPHAKYCIQ VSAQDLTDYG KPSDWSLPGQ VESAPHKP 

« Hide

References

« Hide 'large scale' references
[1]"Mouse homolog of human Epstein-Barr virus-induced gene 3 (EBI3)."
Nomura H., Yaguchi N., Hanyuu C., Maeda M., Kikuchi Y., Nakata Y., Kojima T., Tulin E.E., Hasegawa M.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[3]"The biology and therapeutic potential of interleukin 27."
Batten M., Ghilardi N.
J. Mol. Med. 85:661-672(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF013114 mRNA. Translation: AAB67115.1.
BC008209 mRNA. Translation: AAH08209.1.
CCDSCCDS28888.1.
RefSeqNP_056581.1. NM_015766.2.
UniGeneMm.256798.

3D structure databases

ProteinModelPortalO35228.
SMRO35228. Positions 126-217.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60364N.
IntActO35228. 1 interaction.
STRING10090.ENSMUSP00000003274.

Proteomic databases

PRIDEO35228.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206.
GeneID50498.
KEGGmmu:50498.
UCSCuc008daj.1. mouse.

Organism-specific databases

CTD10148.
MGIMGI:1354171. Ebi3.

Phylogenomic databases

eggNOGNOG43082.
HOGENOMHOG000294168.
HOVERGENHBG052107.
InParanoidO35228.
OMAPTSFIAT.
OrthoDBEOG789CCW.
PhylomeDBO35228.
TreeFamTF331210.

Gene expression databases

ArrayExpressO35228.
BgeeO35228.
CleanExMM_EBI3.
MM_IL17B.
GenevestigatorO35228.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR003961. Fibronectin_type3.
IPR003530. Hematopoietin_rcpt_L_F3_CS.
IPR013783. Ig-like_fold.
IPR015373. Interferon_alpha/beta_rcpt_bsu.
[Graphical view]
PfamPF09294. Interfer-bind. 1 hit.
[Graphical view]
SMARTSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS50853. FN3. 2 hits.
PS01354. HEMATOPO_REC_L_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio307494.
PROO35228.
SOURCESearch...

Entry information

Entry nameIL27B_MOUSE
AccessionPrimary (citable) accession number: O35228
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot