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Protein

Interleukin-27 subunit beta

Gene

Ebi3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.

GO - Molecular functioni

  • cytokine receptor activity Source: MGI
  • interleukin-27 receptor binding Source: MGI

GO - Biological processi

  • cytokine-mediated signaling pathway Source: MGI
  • T cell proliferation Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-27 subunit beta
Short name:
IL-27 subunit beta
Short name:
IL-27B
Alternative name(s):
Epstein-Barr virus-induced gene 3 protein homolog
Gene namesi
Name:Ebi3
Synonyms:Il27b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1354171. Ebi3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 228210Interleukin-27 subunit betaPRO_0000010938Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO35228.

Expressioni

Gene expression databases

BgeeiO35228.
CleanExiMM_EBI3.
MM_IL17B.
ExpressionAtlasiO35228. baseline and differential.
GenevisibleiO35228. MM.

Interactioni

Subunit structurei

Heterodimer with IL27/IL27A; not disulfide-linked. This heterodimer is known as interleukin IL-27. EBI3 is also a component of the IL-12 heterodimer. Interacts with SQSTM1 (By similarity). Heterodimer with IL12A; this heterodimer is known as interleukin IL-35 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-60364N.
IntActiO35228. 1 interaction.
STRINGi10090.ENSMUSP00000003274.

Structurei

3D structure databases

ProteinModelPortaliO35228.
SMRiO35228. Positions 126-217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 12499Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini127 – 22498Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG43082.
GeneTreeiENSGT00530000063103.
HOGENOMiHOG000294168.
HOVERGENiHBG052107.
InParanoidiO35228.
OMAiYPVAVDC.
OrthoDBiEOG789CCW.
PhylomeDBiO35228.
TreeFamiTF331210.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR003530. Hematopoietin_rcpt_L_F3_CS.
IPR013783. Ig-like_fold.
IPR015373. Interferon/interleukin_rcp_dom.
[Graphical view]
PfamiPF09294. Interfer-bind. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS01354. HEMATOPO_REC_L_F3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLLFLSLA LWASRSPGYT ETALVALSQP RVQCHASRYP VAVDCSWTPL
60 70 80 90 100
QAPNSTRSTS FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP
110 120 130 140 150
YMLNVTAVHP GGASSSLLAF VAERIIKPDP PEGVRLRTAG QRLQVLWHPP
160 170 180 190 200
ASWPFPDIFS LKYRLRYRRR GASHFRQVGP IEATTFTLRN SKPHAKYCIQ
210 220
VSAQDLTDYG KPSDWSLPGQ VESAPHKP
Length:228
Mass (Da):25,353
Last modified:January 1, 1998 - v1
Checksum:i49DEFA4E5C4F2126
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013114 mRNA. Translation: AAB67115.1.
BC008209 mRNA. Translation: AAH08209.1.
CCDSiCCDS28888.1.
RefSeqiNP_056581.1. NM_015766.2.
UniGeneiMm.256798.

Genome annotation databases

EnsembliENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206.
GeneIDi50498.
KEGGimmu:50498.
UCSCiuc008daj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013114 mRNA. Translation: AAB67115.1.
BC008209 mRNA. Translation: AAH08209.1.
CCDSiCCDS28888.1.
RefSeqiNP_056581.1. NM_015766.2.
UniGeneiMm.256798.

3D structure databases

ProteinModelPortaliO35228.
SMRiO35228. Positions 126-217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60364N.
IntActiO35228. 1 interaction.
STRINGi10090.ENSMUSP00000003274.

Proteomic databases

PRIDEiO35228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206.
GeneIDi50498.
KEGGimmu:50498.
UCSCiuc008daj.1. mouse.

Organism-specific databases

CTDi10148.
MGIiMGI:1354171. Ebi3.

Phylogenomic databases

eggNOGiNOG43082.
GeneTreeiENSGT00530000063103.
HOGENOMiHOG000294168.
HOVERGENiHBG052107.
InParanoidiO35228.
OMAiYPVAVDC.
OrthoDBiEOG789CCW.
PhylomeDBiO35228.
TreeFamiTF331210.

Miscellaneous databases

NextBioi307494.
PROiO35228.
SOURCEiSearch...

Gene expression databases

BgeeiO35228.
CleanExiMM_EBI3.
MM_IL17B.
ExpressionAtlasiO35228. baseline and differential.
GenevisibleiO35228. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR003530. Hematopoietin_rcpt_L_F3_CS.
IPR013783. Ig-like_fold.
IPR015373. Interferon/interleukin_rcp_dom.
[Graphical view]
PfamiPF09294. Interfer-bind. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS01354. HEMATOPO_REC_L_F3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse homolog of human Epstein-Barr virus-induced gene 3 (EBI3)."
    Nomura H., Yaguchi N., Hanyuu C., Maeda M., Kikuchi Y., Nakata Y., Kojima T., Tulin E.E., Hasegawa M.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  3. "The biology and therapeutic potential of interleukin 27."
    Batten M., Ghilardi N.
    J. Mol. Med. 85:661-672(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiIL27B_MOUSE
AccessioniPrimary (citable) accession number: O35228
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 1, 1998
Last modified: July 22, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.