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Protein

Interleukin-27 subunit beta

Gene

Ebi3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.

GO - Molecular functioni

  • cytokine activity Source: UniProtKB-KW
  • cytokine receptor activity Source: MGI
  • interleukin-27 receptor binding Source: MGI

GO - Biological processi

  • T cell proliferation Source: CACAO

Keywordsi

Molecular functionCytokine

Enzyme and pathway databases

ReactomeiR-MMU-447115. Interleukin-12 family signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-27 subunit beta
Short name:
IL-27 subunit beta
Short name:
IL-27B
Alternative name(s):
Epstein-Barr virus-induced gene 3 protein homolog
Gene namesi
Name:Ebi3
Synonyms:Il27b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1354171. Ebi3.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001093819 – 228Interleukin-27 subunit betaAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi54N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi104N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO35228.
PeptideAtlasiO35228.
PRIDEiO35228.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003206.
CleanExiMM_EBI3.
MM_IL17B.
ExpressionAtlasiO35228. baseline and differential.
GenevisibleiO35228. MM.

Interactioni

Subunit structurei

Heterodimer with IL27/IL27A; not disulfide-linked. This heterodimer is known as interleukin IL-27. EBI3 is also a component of the IL-12 heterodimer. Interacts with SQSTM1 (By similarity). Heterodimer with IL12A; this heterodimer is known as interleukin IL-35 (By similarity).By similarity

GO - Molecular functioni

  • cytokine activity Source: UniProtKB-KW
  • interleukin-27 receptor binding Source: MGI

Protein-protein interaction databases

DIPiDIP-60364N.
IntActiO35228. 1 interactor.
STRINGi10090.ENSMUSP00000003274.

Structurei

3D structure databases

ProteinModelPortaliO35228.
SMRiO35228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 124Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST99
Domaini127 – 224Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IX61. Eukaryota.
ENOG4111KU8. LUCA.
GeneTreeiENSGT00530000063103.
HOGENOMiHOG000294168.
HOVERGENiHBG052107.
InParanoidiO35228.
OMAiFIATYRL.
OrthoDBiEOG091G0P18.
PhylomeDBiO35228.
TreeFamiTF331210.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 2 hits.
InterProiView protein in InterPro
IPR003961. FN3_dom.
IPR003530. Hematopoietin_rcpt_L_F3_CS.
IPR013783. Ig-like_fold.
IPR015373. Interferon/interleukin_rcp_dom.
PfamiView protein in Pfam
PF09294. Interfer-bind. 1 hit.
SMARTiView protein in SMART
SM00060. FN3. 1 hit.
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiView protein in PROSITE
PS50853. FN3. 2 hits.
PS01354. HEMATOPO_REC_L_F3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLLFLSLA LWASRSPGYT ETALVALSQP RVQCHASRYP VAVDCSWTPL
60 70 80 90 100
QAPNSTRSTS FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP
110 120 130 140 150
YMLNVTAVHP GGASSSLLAF VAERIIKPDP PEGVRLRTAG QRLQVLWHPP
160 170 180 190 200
ASWPFPDIFS LKYRLRYRRR GASHFRQVGP IEATTFTLRN SKPHAKYCIQ
210 220
VSAQDLTDYG KPSDWSLPGQ VESAPHKP
Length:228
Mass (Da):25,353
Last modified:January 1, 1998 - v1
Checksum:i49DEFA4E5C4F2126
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013114 mRNA. Translation: AAB67115.1.
BC008209 mRNA. Translation: AAH08209.1.
CCDSiCCDS28888.1.
RefSeqiNP_056581.1. NM_015766.2.
UniGeneiMm.256798.

Genome annotation databases

EnsembliENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206.
GeneIDi50498.
KEGGimmu:50498.
UCSCiuc008daj.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIL27B_MOUSE
AccessioniPrimary (citable) accession number: O35228
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families