O35228 (IL27B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-27 subunit beta
Short name=IL-27 subunit beta
Short name=IL-27B

Alternative name(s):
Epstein-Barr virus-induced gene 3 protein homolog

Gene names

Name:	Ebi3
Synonyms:	Il17b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Subunit structure	Heterodimer with IL27. IL-27 is a heterodimer composed IL27 and EBI3. EBI3 is also a component of the IL-12 heterodimer. Interacts with SQSTM1 By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the type I cytokine receptor family. Type 3 subfamily. Contains 2 fibronectin type-III domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Cytokine
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	cytokine receptor activity Inferred from electronic annotation. Source: Compara interleukin-27 receptor binding Inferred from direct assay PubMed 12121660. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 228	210	Interleukin-27 subunit beta		PRO_0000010938
Regions
	Domain	23 – 123	101	Fibronectin type-III 1
	Domain	128 – 219	92	Fibronectin type-III 2
Amino acid modifications
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O35228 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 49DEFA4E5C4F2126	FASTA	228	25,353
	10 20 30 40 50 60 MSKLLFLSLA LWASRSPGYT ETALVALSQP RVQCHASRYP VAVDCSWTPL QAPNSTRSTS 70 80 90 100 110 120 FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP YMLNVTAVHP GGASSSLLAF 130 140 150 160 170 180 VAERIIKPDP PEGVRLRTAG QRLQVLWHPP ASWPFPDIFS LKYRLRYRRR GASHFRQVGP 190 200 210 220 IEATTFTLRN SKPHAKYCIQ VSAQDLTDYG KPSDWSLPGQ VESAPHKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse homolog of human Epstein-Barr virus-induced gene 3 (EBI3)." Nomura H., Yaguchi N., Hanyuu C., Maeda M., Kikuchi Y., Nakata Y., Kojima T., Tulin E.E., Hasegawa M. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
[3]	"The biology and therapeutic potential of interleukin 27." Batten M., Ghilardi N. J. Mol. Med. 85:661-672(2007) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF013114 mRNA. Translation: AAB67115.1. BC008209 mRNA. Translation: AAH08209.1.
IPI	IPI00128566.
RefSeq	NP_056581.1. NM_015766.2.
UniGene	Mm.256798.
3D structure databases
ProteinModelPortal	O35228.
SMR	O35228. Positions 30-224.
ModBase	Search...
Protein-protein interaction databases
IntAct	O35228. 1 interaction.
STRING	10090.ENSMUSP00000003274.
Proteomic databases
PRIDE	O35228.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206.
GeneID	50498.
KEGG	mmu:50498.
Organism-specific databases
CTD	10148.
MGI	MGI:1354171. Ebi3.
Phylogenomic databases
eggNOG	NOG43082.
HOGENOM	HOG000294168.
HOVERGEN	HBG052107.
InParanoid	O35228.
OMA	PTSFIAT.
OrthoDB	EOG412M6N.
Gene expression databases
ArrayExpress	O35228.
Bgee	O35228.
CleanEx	MM_EBI3. MM_IL17B.
Genevestigator	O35228.
GermOnline	ENSMUSG00000003206. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR003961. Fibronectin_type3. IPR003530. Hematopoietin_rcpt_L_F3_CS. IPR013783. Ig-like_fold. IPR015373. Interferon_alpha/beta_rcpt_bsu. [Graphical view]
Pfam	PF09294. Interfer-bind. 1 hit. [Graphical view]
SMART	SM00060. FN3. 1 hit. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 2 hits.
PROSITE	PS50853. FN3. 2 hits. PS01354. HEMATOPO_REC_L_F3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307494.
SOURCE	Search...

Entry information

Entry name

IL27B_MOUSE

Accession

Primary (citable) accession number: O35228

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program