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Protein

Interleukin-27 subunit beta

Gene

Ebi3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.

GO - Molecular functioni

  • cytokine activity Source: UniProtKB-KW
  • cytokine receptor activity Source: MGI
  • interleukin-27 receptor binding Source: MGI

GO - Biological processi

  • T cell proliferation Source: CACAO

Keywordsi

Molecular functionCytokine

Enzyme and pathway databases

ReactomeiR-MMU-8984722 Interleukin-35 Signalling
R-MMU-9020956 Interleukin-27 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-27 subunit beta
Short name:
IL-27 subunit beta
Short name:
IL-27B
Alternative name(s):
Epstein-Barr virus-induced gene 3 protein homolog
Gene namesi
Name:Ebi3
Synonyms:Il27b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1354171 Ebi3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001093819 – 228Interleukin-27 subunit betaAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi54N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi104N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO35228
PeptideAtlasiO35228
PRIDEiO35228

Expressioni

Gene expression databases

BgeeiENSMUSG00000003206
CleanExiMM_EBI3
MM_IL17B
ExpressionAtlasiO35228 baseline and differential
GenevisibleiO35228 MM

Interactioni

Subunit structurei

Heterodimer with IL27/IL27A; not disulfide-linked. This heterodimer is known as interleukin IL-27. EBI3 is also a component of the IL-12 heterodimer. Interacts with SQSTM1 (By similarity). Heterodimer with IL12A; this heterodimer is known as interleukin IL-35 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Il12aP434312EBI-3862967,EBI-3862959

GO - Molecular functioni

  • cytokine activity Source: UniProtKB-KW
  • interleukin-27 receptor binding Source: MGI

Protein-protein interaction databases

DIPiDIP-60364N
IntActiO35228, 1 interactor
STRINGi10090.ENSMUSP00000003274

Structurei

3D structure databases

ProteinModelPortaliO35228
SMRiO35228
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 124Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST99
Domaini127 – 224Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IX61 Eukaryota
ENOG4111KU8 LUCA
GeneTreeiENSGT00530000063103
HOGENOMiHOG000294168
HOVERGENiHBG052107
InParanoidiO35228
OMAiFIATYRL
OrthoDBiEOG091G0P18
PhylomeDBiO35228
TreeFamiTF331210

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR003530 Hematopoietin_rcpt_L_F3_CS
IPR013783 Ig-like_fold
IPR015373 Interferon/interleukin_rcp_dom
PfamiView protein in Pfam
PF09294 Interfer-bind, 1 hit
SMARTiView protein in SMART
SM00060 FN3, 1 hit
SUPFAMiSSF49265 SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 2 hits
PS01354 HEMATOPO_REC_L_F3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLLFLSLA LWASRSPGYT ETALVALSQP RVQCHASRYP VAVDCSWTPL
60 70 80 90 100
QAPNSTRSTS FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP
110 120 130 140 150
YMLNVTAVHP GGASSSLLAF VAERIIKPDP PEGVRLRTAG QRLQVLWHPP
160 170 180 190 200
ASWPFPDIFS LKYRLRYRRR GASHFRQVGP IEATTFTLRN SKPHAKYCIQ
210 220
VSAQDLTDYG KPSDWSLPGQ VESAPHKP
Length:228
Mass (Da):25,353
Last modified:January 1, 1998 - v1
Checksum:i49DEFA4E5C4F2126
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013114 mRNA Translation: AAB67115.1
BC008209 mRNA Translation: AAH08209.1
CCDSiCCDS28888.1
RefSeqiNP_056581.1, NM_015766.2
UniGeneiMm.256798

Genome annotation databases

EnsembliENSMUST00000003274; ENSMUSP00000003274; ENSMUSG00000003206
GeneIDi50498
KEGGimmu:50498
UCSCiuc008daj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiIL27B_MOUSE
AccessioniPrimary (citable) accession number: O35228
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health