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O35217

- MINP1_RAT

UniProt

O35217 - MINP1_RAT

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Protein
Multiple inositol polyphosphate phosphatase 1
Gene
Minpp1, Mipp1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a phosphoinositide 5- and phosphoinositide 6-phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3-phosphoglycerate. May play a role in bone development (endochondral ossification).1 Publication

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = myo-inositol pentakisphosphate (mixed isomers) + phosphate.1 Publication
2,3-bisphospho-D-glycerate + H2O = 2-phospho-D-glycerate + phosphate.1 Publication

Kineticsi

  1. KM=6.9 µM for Ins(1,3,4,5)P41 Publication

Vmax=302 pmol/min/µg enzyme

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891 Reviewed prediction

GO - Molecular functioni

  1. acid phosphatase activity Source: InterPro
  2. bisphosphoglycerate 3-phosphatase activity Source: UniProtKB-EC
  3. inositol hexakisphosphate 2-phosphatase activity Source: UniProtKB-EC
  4. inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity Source: RGD
  5. inositol-1,4,5,6-tetrakisphosphate 6-phosphatase activity Source: RGD

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. phosphatidylinositol-mediated signaling Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Multiple inositol polyphosphate phosphatase 1 (EC:3.1.3.62)
Alternative name(s):
2,3-bisphosphoglycerate 3-phosphatase (EC:3.1.3.80)
Short name:
2,3-BPG phosphatase
Inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase
Short name:
Ins(1,3,4,5)P(4) 3-phosphatase
Gene namesi
Name:Minpp1
Synonyms:Mipp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3089. Minpp1.

Subcellular locationi

Endoplasmic reticulum lumen 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030 Reviewed prediction
Add
BLAST
Chaini31 – 481451Multiple inositol polyphosphate phosphatase 1
PRO_0000019585Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi236 – 2361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi475 – 4751N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO35217.

Expressioni

Tissue specificityi

Widely expressed with highest levels in kidney and liver. Expressed in chondrocytes with an elevated expression in hypertrophic chondrocytes.2 Publications

Gene expression databases

GenevestigatoriO35217.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015017.

Structurei

3D structure databases

ProteinModelPortaliO35217.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi478 – 4814Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG260296.
HOGENOMiHOG000113591.
HOVERGENiHBG052872.
InParanoidiO35217.
KOiK03103.
PhylomeDBiO35217.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35217-1 [UniParc]FASTAAdd to Basket

« Hide

MLRGARSHLS ASVALAAVLA AALLSSFARC SLPGRGDPVA SVLSPYFGTK    50
TRYEDVNPWL LGDPVAPRRD PELLAGTCTP VQLVALIRHG TRYPTTKQIR 100
KLRQLQGLLQ TRESVDGGSR VAAALDQWPL WYDDWMDGQL VEKGRQDMRQ 150
LALRLAALFP DLFCRENYGR LRLITSSKHR CVDSSAAFLQ GLWQHYHPGL 200
PPPDVSDMEC DPPRVNDKLM RFFDHCEKFL TEVERNATAL YHVEAFKTGP 250
EMQTVLKKVA ATLQVPVNNL NADLIQVAFF TCSFDLAIQG VHSPWCDVFD 300
VDDAKVLEYL NDLKQYWKRS YGYAINSRSS CNLFQDIFLH LDKAVEQKQR 350
SQPVSSSVIL QFGHAETLLP LLSLMGYFKD KEPLTAYNFE EQVHREFRSG 400
HIVPYASNLI FVLYHCEDAQ TPQEKFQIQM LLNEKVLPLA HSQKTVALYE 450
DLKNHYQDIL QSCQTSKECN LPKVNITSDE L 481
Length:481
Mass (Da):54,589
Last modified:June 1, 2003 - v3
Checksum:i44E7BC000129DB47
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331P → H AA sequence 1 Publication
Sequence conflicti357 – 3571S → P AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012714 mRNA. Translation: AAC53453.2.
RefSeqiNP_062136.1. NM_019263.1.
UniGeneiRn.27882.

Genome annotation databases

GeneIDi29688.
KEGGirno:29688.
UCSCiRGD:3089. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012714 mRNA. Translation: AAC53453.2 .
RefSeqi NP_062136.1. NM_019263.1.
UniGenei Rn.27882.

3D structure databases

ProteinModelPortali O35217.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000015017.

Proteomic databases

PRIDEi O35217.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29688.
KEGGi rno:29688.
UCSCi RGD:3089. rat.

Organism-specific databases

CTDi 9562.
RGDi 3089. Minpp1.

Phylogenomic databases

eggNOGi NOG260296.
HOGENOMi HOG000113591.
HOVERGENi HBG052872.
InParanoidi O35217.
KOi K03103.
PhylomeDBi O35217.

Miscellaneous databases

NextBioi 610065.
PROi O35217.

Gene expression databases

Genevestigatori O35217.

Family and domain databases

Gene3Di 3.40.50.1240. 1 hit.
InterProi IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view ]
Pfami PF00328. His_Phos_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMi SSF53254. SSF53254. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of a rat hepatic multiple inositol polyphosphate phosphatase."
    Craxton A., Caffrey J.J., Burkhart W., Safrany S.T., Shears S.B.
    Biochem. J. 328:75-81(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "Hepatic Ins(1,3,4,5)P4 3-phosphatase is compartmentalized inside endoplasmic reticulum."
    Ali N., Craxton A., Shears S.B.
    J. Biol. Chem. 268:6161-6167(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification."
    Caffrey J.J., Hidaka K., Matsuda M., Hirata M., Shears S.B.
    FEBS Lett. 442:99-104(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
    Tissue: Liver.

Entry informationi

Entry nameiMINP1_RAT
AccessioniPrimary (citable) accession number: O35217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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