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Protein

Histone H3-like centromeric protein A

Gene

Cenpa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_286371. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_306375. Mitotic Prometaphase.
REACT_321346. Separation of Sister Chromatids.
REACT_329805. Resolution of Sister Chromatid Cohesion.
REACT_358264. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H3-like centromeric protein A
Alternative name(s):
Centromere protein A
Short name:
CENP-A
Gene namesi
Name:Cenpa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:88375. Cenpa.

Subcellular locationi

  • Nucleus
  • Chromosomecentromerekinetochore

  • Note: Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 134134Histone H3-like centromeric protein APRO_0000221374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei22 – 221PhosphoserineBy similarity

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.

Keywords - PTMi

ADP-ribosylation, Phosphoprotein

Proteomic databases

MaxQBiO35216.
PRIDEiO35216.

PTM databases

PhosphoSiteiO35216.

Expressioni

Gene expression databases

BgeeiO35216.
CleanExiMM_CENPA.
ExpressionAtlasiO35216. baseline and differential.
GenevisibleiO35216. MM.

Interactioni

Subunit structurei

Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts (via CATD domain) with HJURP; the interaction is direct and is required for its localization to centromeres. Interacts with CENPC, CENPN and CENPT; interaction is direct. Part of a centromere complex consisting of CENPA, CENPT and CENPW (By similarity).By similarity

Protein-protein interaction databases

IntActiO35216. 1 interaction.
MINTiMINT-237454.
STRINGi10090.ENSMUSP00000122831.

Structurei

3D structure databases

ProteinModelPortaliO35216.
SMRiO35216. Positions 54-128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 134100H3-likeAdd
BLAST
Regioni69 – 11042CATDAdd
BLAST

Domaini

The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.By similarity

Sequence similaritiesi

Belongs to the histone H3 family.Curated

Phylogenomic databases

eggNOGiCOG2036.
GeneTreeiENSGT00760000119422.
HOVERGENiHBG001172.
InParanoidiO35216.
KOiK11495.
OMAiHLLIRKY.
PhylomeDBiO35216.
TreeFamiTF354293.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR000164. Histone_H3/CENP-A.
[Graphical view]
PANTHERiPTHR11426. PTHR11426. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00622. HISTONEH3.
SMARTiSM00428. H3. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

O35216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPRRKPQTP RRRPSSPAPG PSRQSSSVGS QTLRRRQKFM WLKEIKTLQK
60 70 80 90 100
STDLLFRKKP FSMVVREICE KFSRGVDFWW QAQALLALQE AAEAFLIHLF
110 120 130
EDAYLLSLHA GRVTLFPKDI QLTRRIRGFE GGLP
Length:134
Mass (Da):15,509
Last modified:January 1, 1998 - v1
Checksum:i0381FA6492E43C3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012709 mRNA. Translation: AAC39957.1.
AF012710 Genomic DNA. Translation: AAC39958.1.
AK010718 mRNA. Translation: BAB27140.1.
AK011399 mRNA. Translation: BAB27591.1.
AK053763 mRNA. Translation: BAC35512.1.
AK089033 mRNA. Translation: BAC40710.1.
BC011038 mRNA. Translation: AAH11038.1.
CCDSiCCDS19162.1.
RefSeqiNP_001289058.1. NM_001302129.1.
NP_001289059.1. NM_001302130.1.
NP_001289060.1. NM_001302131.1.
NP_001289061.1. NM_001302132.1.
NP_031707.1. NM_007681.3.
UniGeneiMm.290563.

Genome annotation databases

EnsembliENSMUST00000144742; ENSMUSP00000122831; ENSMUSG00000029177.
GeneIDi12615.
KEGGimmu:12615.
UCSCiuc008wvt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012709 mRNA. Translation: AAC39957.1.
AF012710 Genomic DNA. Translation: AAC39958.1.
AK010718 mRNA. Translation: BAB27140.1.
AK011399 mRNA. Translation: BAB27591.1.
AK053763 mRNA. Translation: BAC35512.1.
AK089033 mRNA. Translation: BAC40710.1.
BC011038 mRNA. Translation: AAH11038.1.
CCDSiCCDS19162.1.
RefSeqiNP_001289058.1. NM_001302129.1.
NP_001289059.1. NM_001302130.1.
NP_001289060.1. NM_001302131.1.
NP_001289061.1. NM_001302132.1.
NP_031707.1. NM_007681.3.
UniGeneiMm.290563.

3D structure databases

ProteinModelPortaliO35216.
SMRiO35216. Positions 54-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35216. 1 interaction.
MINTiMINT-237454.
STRINGi10090.ENSMUSP00000122831.

PTM databases

PhosphoSiteiO35216.

Proteomic databases

MaxQBiO35216.
PRIDEiO35216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000144742; ENSMUSP00000122831; ENSMUSG00000029177.
GeneIDi12615.
KEGGimmu:12615.
UCSCiuc008wvt.2. mouse.

Organism-specific databases

CTDi1058.
MGIiMGI:88375. Cenpa.

Phylogenomic databases

eggNOGiCOG2036.
GeneTreeiENSGT00760000119422.
HOVERGENiHBG001172.
InParanoidiO35216.
KOiK11495.
OMAiHLLIRKY.
PhylomeDBiO35216.
TreeFamiTF354293.

Enzyme and pathway databases

ReactomeiREACT_286371. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_306375. Mitotic Prometaphase.
REACT_321346. Separation of Sister Chromatids.
REACT_329805. Resolution of Sister Chromatid Cohesion.
REACT_358264. RHO GTPases Activate Formins.

Miscellaneous databases

NextBioi281778.
PROiO35216.
SOURCEiSearch...

Gene expression databases

BgeeiO35216.
CleanExiMM_CENPA.
ExpressionAtlasiO35216. baseline and differential.
GenevisibleiO35216. MM.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR000164. Histone_H3/CENP-A.
[Graphical view]
PANTHERiPTHR11426. PTHR11426. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00622. HISTONEH3.
SMARTiSM00428. H3. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure and sequence analysis of mouse centromere proteins A and C."
    Kalitsis P., Macdonald A.C., Newson A.J., Hudson D.F., Choo K.H.A.
    Genomics 47:108-114(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/Sv and C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Eye and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated."
    Saxena A., Saffery R., Wong L.H., Kalitsis P., Choo K.H.
    J. Biol. Chem. 277:26921-26926(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: POLY-ADP-RIBOSYLATION BY PARP1.

Entry informationi

Entry nameiCENPA_MOUSE
AccessioniPrimary (citable) accession number: O35216
Secondary accession number(s): Q545C9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: July 22, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.