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O35216

- CENPA_MOUSE

UniProt

O35216 - CENPA_MOUSE

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Protein

Histone H3-like centromeric protein A

Gene

Cenpa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro) (By similarity).By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. establishment of mitotic spindle orientation Source: Ensembl
  2. kinetochore assembly Source: Ensembl
  3. protein localization to chromosome, centromeric region Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198597. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H3-like centromeric protein A
Alternative name(s):
Centromere protein A
Short name:
CENP-A
Gene namesi
Name:Cenpa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:88375. Cenpa.

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (By similarity).By similarity

GO - Cellular componenti

  1. chromosome, centromeric region Source: MGI
  2. condensed chromosome inner kinetochore Source: MGI
  3. condensed nuclear chromosome kinetochore Source: Ensembl
  4. nucleosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 134134Histone H3-like centromeric protein APRO_0000221374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei22 – 221PhosphoserineBy similarity

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.

Keywords - PTMi

ADP-ribosylation, Phosphoprotein

Proteomic databases

MaxQBiO35216.
PRIDEiO35216.

PTM databases

PhosphoSiteiO35216.

Expressioni

Gene expression databases

BgeeiO35216.
CleanExiMM_CENPA.
ExpressionAtlasiO35216. baseline and differential.
GenevestigatoriO35216.

Interactioni

Subunit structurei

Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts (via CATD domain) with HJURP; the interaction is direct and is required for its localization to centromeres. Interacts with CENPC, CENPN and CENPT; interaction is direct. Part of a centromere complex consisting of CENPA, CENPT and CENPW (By similarity).By similarity

Protein-protein interaction databases

IntActiO35216. 1 interaction.
MINTiMINT-237454.
STRINGi10090.ENSMUSP00000122831.

Structurei

3D structure databases

ProteinModelPortaliO35216.
SMRiO35216. Positions 54-128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 134100H3-likeAdd
BLAST
Regioni69 – 11042CATDAdd
BLAST

Domaini

The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.By similarity

Sequence similaritiesi

Belongs to the histone H3 family.Curated

Phylogenomic databases

eggNOGiCOG2036.
GeneTreeiENSGT00760000119422.
HOVERGENiHBG001172.
InParanoidiO35216.
KOiK11495.
OMAiLVREICV.
PhylomeDBiO35216.
TreeFamiTF354293.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR000164. Histone_H3/CENP-A.
[Graphical view]
PANTHERiPTHR11426. PTHR11426. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00622. HISTONEH3.
SMARTiSM00428. H3. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

O35216-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGPRRKPQTP RRRPSSPAPG PSRQSSSVGS QTLRRRQKFM WLKEIKTLQK
60 70 80 90 100
STDLLFRKKP FSMVVREICE KFSRGVDFWW QAQALLALQE AAEAFLIHLF
110 120 130
EDAYLLSLHA GRVTLFPKDI QLTRRIRGFE GGLP
Length:134
Mass (Da):15,509
Last modified:January 1, 1998 - v1
Checksum:i0381FA6492E43C3D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012709 mRNA. Translation: AAC39957.1.
AF012710 Genomic DNA. Translation: AAC39958.1.
AK010718 mRNA. Translation: BAB27140.1.
AK011399 mRNA. Translation: BAB27591.1.
AK053763 mRNA. Translation: BAC35512.1.
AK089033 mRNA. Translation: BAC40710.1.
BC011038 mRNA. Translation: AAH11038.1.
CCDSiCCDS19162.1.
RefSeqiNP_031707.1. NM_007681.2.
UniGeneiMm.290563.

Genome annotation databases

EnsembliENSMUST00000144742; ENSMUSP00000122831; ENSMUSG00000029177.
GeneIDi12615.
KEGGimmu:12615.
UCSCiuc008wvt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012709 mRNA. Translation: AAC39957.1 .
AF012710 Genomic DNA. Translation: AAC39958.1 .
AK010718 mRNA. Translation: BAB27140.1 .
AK011399 mRNA. Translation: BAB27591.1 .
AK053763 mRNA. Translation: BAC35512.1 .
AK089033 mRNA. Translation: BAC40710.1 .
BC011038 mRNA. Translation: AAH11038.1 .
CCDSi CCDS19162.1.
RefSeqi NP_031707.1. NM_007681.2.
UniGenei Mm.290563.

3D structure databases

ProteinModelPortali O35216.
SMRi O35216. Positions 54-128.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O35216. 1 interaction.
MINTi MINT-237454.
STRINGi 10090.ENSMUSP00000122831.

PTM databases

PhosphoSitei O35216.

Proteomic databases

MaxQBi O35216.
PRIDEi O35216.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000144742 ; ENSMUSP00000122831 ; ENSMUSG00000029177 .
GeneIDi 12615.
KEGGi mmu:12615.
UCSCi uc008wvt.1. mouse.

Organism-specific databases

CTDi 1058.
MGIi MGI:88375. Cenpa.

Phylogenomic databases

eggNOGi COG2036.
GeneTreei ENSGT00760000119422.
HOVERGENi HBG001172.
InParanoidi O35216.
KOi K11495.
OMAi LVREICV.
PhylomeDBi O35216.
TreeFami TF354293.

Enzyme and pathway databases

Reactomei REACT_198597. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Miscellaneous databases

NextBioi 281778.
PROi O35216.
SOURCEi Search...

Gene expression databases

Bgeei O35216.
CleanExi MM_CENPA.
ExpressionAtlasi O35216. baseline and differential.
Genevestigatori O35216.

Family and domain databases

Gene3Di 1.10.20.10. 1 hit.
InterProi IPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR000164. Histone_H3/CENP-A.
[Graphical view ]
PANTHERi PTHR11426. PTHR11426. 1 hit.
Pfami PF00125. Histone. 1 hit.
[Graphical view ]
PRINTSi PR00622. HISTONEH3.
SMARTi SM00428. H3. 1 hit.
[Graphical view ]
SUPFAMi SSF47113. SSF47113. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure and sequence analysis of mouse centromere proteins A and C."
    Kalitsis P., Macdonald A.C., Newson A.J., Hudson D.F., Choo K.H.A.
    Genomics 47:108-114(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/Sv and C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Eye and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated."
    Saxena A., Saffery R., Wong L.H., Kalitsis P., Choo K.H.
    J. Biol. Chem. 277:26921-26926(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: POLY-ADP-RIBOSYLATION BY PARP1.

Entry informationi

Entry nameiCENPA_MOUSE
AccessioniPrimary (citable) accession number: O35216
Secondary accession number(s): Q545C9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3