O35214 (OPSX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Visual pigment-like receptor peropsin

Gene names

Name:

Rrh

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in rpe physiology either by detecting light directly or by monitoring the concentration of retinoids or other photoreceptor-derived compounds.
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Found only in the eye, where it is localized to the retinal pigment epithelium (RPE). In the RPE, it is localized to the microvilli that surround the photoreceptor outer segments.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Visual pigment-like receptor peropsin		PRO_0000197820
Regions
	Topological domain	1 – 26	26	Extracellular
	Transmembrane	27 – 49	23	Helical; Name=1; Potential
	Topological domain	50 – 61	12	Cytoplasmic
	Transmembrane	62 – 87	26	Helical; Name=2; Potential
	Topological domain	88 – 101	14	Extracellular
	Transmembrane	102 – 121	20	Helical; Name=3; Potential
	Topological domain	122 – 140	19	Cytoplasmic
	Transmembrane	141 – 164	24	Helical; Name=4; Potential
	Topological domain	165 – 188	24	Extracellular
	Transmembrane	189 – 212	24	Helical; Name=5; Potential
	Topological domain	213 – 240	28	Cytoplasmic
	Transmembrane	241 – 264	24	Helical; Name=6; Potential
	Topological domain	265 – 272	8	Extracellular
	Transmembrane	273 – 297	25	Helical; Name=7; Potential
	Topological domain	298 – 337	40	Cytoplasmic
Amino acid modifications
	Modified residue	284	1	N6-(retinylidene)lysine
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Disulfide bond	98 ↔ 175		Potential

Sequences

	Sequence		Length	Mass (Da)
	O35214 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 353E4E2278EDBFA6	FASTA	337	37,209
	10 20 30 40 50 60 MLSEASDFNS SGSRSEGSVF SRTEHSVIAA YLIVAGITSI LSNVVVLGIF IKYKELRTPT 70 80 90 100 110 120 NAVIINLAFT DIGVSSIGYP MSAASDLHGS WKFGHAGCQI YAGLNIFFGM VSIGLLTVVA 130 140 150 160 170 180 MDRYLTISCP DVGRRMTTNT YLSMILGAWI NGLFWALMPI IGWASYAPDP TGATCTINWR 190 200 210 220 230 240 NNDTSFVSYT MMVIVVNFIV PLTVMFYCYY HVSRSLRLYA ASDCTAHLHR DWADQADVTK 250 260 270 280 290 300 MSVIMILMFL LAWSPYSIVC LWACFGNPKK IPPSMAIIAP LFAKSSTFYN PCIYVAAHKK 310 320 330 FRKAMLAMFK CQPHLAVPEP STLPMDMPQS SLAPVRI

References

[1]	"Peropsin, a novel visual pigment-like protein located in the apical microvilli of the retinal pigment epithelium." Sun H., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J. Proc. Natl. Acad. Sci. U.S.A. 94:9893-9898(1997) [PubMed: 9275222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF012271 mRNA. Translation: AAC53344.1.
IPI	IPI00128521.
RefSeq	NP_033128.1. NM_009102.3.
UniGene	Mm.278758.
3D structure databases
ProteinModelPortal	O35214.
SMR	O35214. Positions 23-335.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	O35214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029648; ENSMUSP00000029648; ENSMUSG00000028012. ENSMUST00000171313; ENSMUSP00000132360; ENSMUSG00000028012.
GeneID	20132.
KEGG	mmu:20132.
Organism-specific databases
CTD	10692.
MGI	MGI:1097709. Rrh.
Phylogenomic databases
eggNOG	roNOG09768.
HOVERGEN	HBG098035.
Gene expression databases
ArrayExpress	O35214.
Bgee	O35214.
Genevestigator	O35214.
GermOnline	ENSMUSG00000028012. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001760. Opsin. IPR002962. Peropsin. [Graphical view]
KO	K04253.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01244. PEROPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	297639.
SOURCE	Search...

Entry information

Entry name

OPSX_MOUSE

Accession

Primary (citable) accession number: O35214

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program