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Protein

Fractalkine

Gene

Cx3cl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (By similarity). Binds to CX3CR1.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei337 – 3382Cleavage; to produce soluble formSequence Analysis

GO - Molecular functioni

  1. chemokine activity Source: MGI

GO - Biological processi

  1. angiogenesis involved in wound healing Source: MGI
  2. cell adhesion Source: UniProtKB-KW
  3. chemotaxis Source: UniProtKB
  4. cytokine-mediated signaling pathway Source: MGI
  5. immune response Source: InterPro
  6. lymphocyte chemotaxis Source: MGI
  7. macrophage chemotaxis Source: MGI
  8. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  9. neutrophil chemotaxis Source: MGI
  10. positive regulation of angiogenesis Source: MGI
  11. positive regulation of calcium-independent cell-cell adhesion Source: MGI
  12. positive regulation of inflammatory response Source: Ensembl
  13. positive regulation of transforming growth factor beta1 production Source: MGI
  14. wound healing Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Cell adhesion, Chemotaxis

Enzyme and pathway databases

ReactomeiREACT_230248. Chemokine receptors bind chemokines.

Names & Taxonomyi

Protein namesi
Recommended name:
Fractalkine
Alternative name(s):
C-X3-C motif chemokine 1
CX3C membrane-anchored chemokine
Neurotactin
Small-inducible cytokine D1
Cleaved into the following chain:
Gene namesi
Name:Cx3cl1
Synonyms:Cx3c, Fkn, Scyd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1097153. Cx3cl1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 336312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei337 – 35721HelicalSequence AnalysisAdd
BLAST
Topological domaini358 – 39538CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. extracellular region Source: MGI
  3. extracellular space Source: UniProtKB-KW
  4. integral component of membrane Source: MGI
  5. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 395371FractalkinePRO_0000005253Add
BLAST
Chaini25 – ?337313Processed fractalkinePRO_0000296225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 58By similarity
Disulfide bondi36 ↔ 74By similarity

Post-translational modificationi

A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiO35188.
PaxDbiO35188.
PRIDEiO35188.

PTM databases

PhosphoSiteiO35188.

Miscellaneous databases

PMAP-CutDBQ91V44.

Expressioni

Tissue specificityi

Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

Gene expression databases

BgeeiO35188.
CleanExiMM_CX3CL1.
ExpressionAtlasiO35188. baseline and differential.
GenevestigatoriO35188.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiO35188. 1 interaction.
MINTiMINT-4120736.

Structurei

3D structure databases

ProteinModelPortaliO35188.
SMRiO35188. Positions 32-97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 10076ChemokineAdd
BLAST
Regioni101 – 336236Mucin-like stalkAdd
BLAST

Sequence similaritiesi

Belongs to the intercrine delta family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46337.
GeneTreeiENSGT00510000049483.
HOGENOMiHOG000036946.
HOVERGENiHBG057269.
InParanoidiO35188.
KOiK05508.
OMAiYQSLQGC.
OrthoDBiEOG75TMD4.
TreeFamiTF337534.

Family and domain databases

InterProiIPR008097. Chemokine_CX3CL1.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR01721. FRACTALKINE.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35188-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPSPLAWLL RLAAFFHLCT LLPGQHLGMT KCEIMCDKMT SRIPVALLIR
60 70 80 90 100
YQLNQESCGK RAIVLETTQH RRFCADPKEK WVQDAMKHLD HQAAALTKNG
110 120 130 140 150
GKFEKRVDNV TPGITLATRG LSPSALTKPE SATLEDLALE LTTISQEARG
160 170 180 190 200
TMGTSQEPPA AVTGSSLSTS EAQDAGLTAK PQSIGSFEAA DISTTVWPSP
210 220 230 240 250
AVYQSGSSSW AEEKATESPS TTAPSPQVST TSPSTPEENV GSEGQPPWVQ
260 270 280 290 300
GQDLSPEKSL GSEEINPVHT DNFQERGPGN TVHPSVAPIS SEETPSPELV
310 320 330 340 350
ASGSQAPKIE EPIHATADPQ KLSVLITPVP DTQAATRRQA VGLLAFLGLL
360 370 380 390
FCLGVAMFAY QSLQGCPRKM AGEMVEGLRY VPRSCGSNSY VLVPV
Length:395
Mass (Da):42,099
Last modified:July 27, 2011 - v3
Checksum:iB5A16F8BD4B80340
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371D → G in AAB71763. (PubMed:9479488)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010586 mRNA. Translation: AAB66331.1.
U92565 mRNA. Translation: AAB71763.1.
AF071549 mRNA. Translation: AAD41620.1.
AK138214 mRNA. Translation: BAE23585.1.
AK138472 mRNA. Translation: BAE23673.1.
AK138574 mRNA. Translation: BAE23702.1.
AK154646 mRNA. Translation: BAE32737.1.
CH466525 Genomic DNA. Translation: EDL11144.1.
BC006650 mRNA. Translation: AAH06650.1.
BC054838 mRNA. Translation: AAH54838.1.
CCDSiCCDS22548.1.
RefSeqiNP_033168.2. NM_009142.3.
UniGeneiMm.103711.

Genome annotation databases

EnsembliENSMUST00000034230; ENSMUSP00000034230; ENSMUSG00000031778.
GeneIDi20312.
KEGGimmu:20312.
UCSCiuc009mwx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010586 mRNA. Translation: AAB66331.1.
U92565 mRNA. Translation: AAB71763.1.
AF071549 mRNA. Translation: AAD41620.1.
AK138214 mRNA. Translation: BAE23585.1.
AK138472 mRNA. Translation: BAE23673.1.
AK138574 mRNA. Translation: BAE23702.1.
AK154646 mRNA. Translation: BAE32737.1.
CH466525 Genomic DNA. Translation: EDL11144.1.
BC006650 mRNA. Translation: AAH06650.1.
BC054838 mRNA. Translation: AAH54838.1.
CCDSiCCDS22548.1.
RefSeqiNP_033168.2. NM_009142.3.
UniGeneiMm.103711.

3D structure databases

ProteinModelPortaliO35188.
SMRiO35188. Positions 32-97.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35188. 1 interaction.
MINTiMINT-4120736.

PTM databases

PhosphoSiteiO35188.

Proteomic databases

MaxQBiO35188.
PaxDbiO35188.
PRIDEiO35188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034230; ENSMUSP00000034230; ENSMUSG00000031778.
GeneIDi20312.
KEGGimmu:20312.
UCSCiuc009mwx.1. mouse.

Organism-specific databases

CTDi6376.
MGIiMGI:1097153. Cx3cl1.

Phylogenomic databases

eggNOGiNOG46337.
GeneTreeiENSGT00510000049483.
HOGENOMiHOG000036946.
HOVERGENiHBG057269.
InParanoidiO35188.
KOiK05508.
OMAiYQSLQGC.
OrthoDBiEOG75TMD4.
TreeFamiTF337534.

Enzyme and pathway databases

ReactomeiREACT_230248. Chemokine receptors bind chemokines.

Miscellaneous databases

NextBioi298087.
PMAP-CutDBQ91V44.
PROiO35188.
SOURCEiSearch...

Gene expression databases

BgeeiO35188.
CleanExiMM_CX3CL1.
ExpressionAtlasiO35188. baseline and differential.
GenevestigatoriO35188.

Family and domain databases

InterProiIPR008097. Chemokine_CX3CL1.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR01721. FRACTALKINE.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of a new type of mouse chemokine."
    Rossi D.L., Hardiman G., Copeland N.G., Gilbert D.J., Jenkins N., Zlotnik A., Bazan J.F.
    Genomics 47:163-170(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Brain.
  3. "Fractalkine and macrophage-derived chemokine: T cell-attracting chemokines expressed in T cell area dendritic cells."
    Kanazawa N., Nakamura T., Tashiro K., Muramatsu M., Morita K., Yoneda K., Inaba K., Imamura S., Honjo T.
    Eur. J. Immunol. 29:1925-1932(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Hypothalamus and Spinal cord.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Eye and Mammary tumor.
  7. Cited for: INDUCTION.

Entry informationi

Entry nameiX3CL1_MOUSE
AccessioniPrimary (citable) accession number: O35188
Secondary accession number(s): O35933, Q91V44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.