Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O35188

- X3CL1_MOUSE

UniProt

O35188 - X3CL1_MOUSE

Protein

Fractalkine

Gene

Cx3cl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium By similarity. Binds to CX3CR1.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei337 – 3382Cleavage; to produce soluble formSequence Analysis

    GO - Molecular functioni

    1. chemokine activity Source: MGI

    GO - Biological processi

    1. angiogenesis involved in wound healing Source: MGI
    2. cell adhesion Source: UniProtKB-KW
    3. chemotaxis Source: UniProtKB
    4. cytokine-mediated signaling pathway Source: MGI
    5. immune response Source: InterPro
    6. lymphocyte chemotaxis Source: MGI
    7. macrophage chemotaxis Source: MGI
    8. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
    9. neutrophil chemotaxis Source: MGI
    10. positive regulation of angiogenesis Source: MGI
    11. positive regulation of calcium-independent cell-cell adhesion Source: Ensembl
    12. positive regulation of inflammatory response Source: Ensembl
    13. positive regulation of transforming growth factor beta1 production Source: MGI
    14. wound healing Source: MGI

    Keywords - Molecular functioni

    Cytokine

    Keywords - Biological processi

    Cell adhesion, Chemotaxis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fractalkine
    Alternative name(s):
    C-X3-C motif chemokine 1
    CX3C membrane-anchored chemokine
    Neurotactin
    Small-inducible cytokine D1
    Cleaved into the following chain:
    Gene namesi
    Name:Cx3cl1
    Synonyms:Cx3c, Fkn, Scyd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1097153. Cx3cl1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. extracellular space Source: UniProtKB-KW
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 395371FractalkinePRO_0000005253Add
    BLAST
    Chaini25 – ?337313Processed fractalkinePRO_0000296225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 58By similarity
    Disulfide bondi36 ↔ 74By similarity

    Post-translational modificationi

    A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiO35188.
    PRIDEiO35188.

    PTM databases

    PhosphoSiteiO35188.

    Miscellaneous databases

    PMAP-CutDBQ91V44.

    Expressioni

    Tissue specificityi

    Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

    Gene expression databases

    BgeeiO35188.
    CleanExiMM_CX3CL1.
    GenevestigatoriO35188.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    IntActiO35188. 1 interaction.
    MINTiMINT-4120736.

    Structurei

    3D structure databases

    ProteinModelPortaliO35188.
    SMRiO35188. Positions 32-97.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 336312ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini358 – 39538CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei337 – 35721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 10076ChemokineAdd
    BLAST
    Regioni101 – 336236Mucin-like stalkAdd
    BLAST

    Sequence similaritiesi

    Belongs to the intercrine delta family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46337.
    GeneTreeiENSGT00510000049483.
    HOGENOMiHOG000036946.
    HOVERGENiHBG057269.
    InParanoidiQ91V44.
    KOiK05508.
    OMAiYQSLQGC.
    OrthoDBiEOG75TMD4.
    TreeFamiTF337534.

    Family and domain databases

    InterProiIPR008097. Chemokine_CX3CL1.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    PRINTSiPR01721. FRACTALKINE.
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35188-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPSPLAWLL RLAAFFHLCT LLPGQHLGMT KCEIMCDKMT SRIPVALLIR    50
    YQLNQESCGK RAIVLETTQH RRFCADPKEK WVQDAMKHLD HQAAALTKNG 100
    GKFEKRVDNV TPGITLATRG LSPSALTKPE SATLEDLALE LTTISQEARG 150
    TMGTSQEPPA AVTGSSLSTS EAQDAGLTAK PQSIGSFEAA DISTTVWPSP 200
    AVYQSGSSSW AEEKATESPS TTAPSPQVST TSPSTPEENV GSEGQPPWVQ 250
    GQDLSPEKSL GSEEINPVHT DNFQERGPGN TVHPSVAPIS SEETPSPELV 300
    ASGSQAPKIE EPIHATADPQ KLSVLITPVP DTQAATRRQA VGLLAFLGLL 350
    FCLGVAMFAY QSLQGCPRKM AGEMVEGLRY VPRSCGSNSY VLVPV 395
    Length:395
    Mass (Da):42,099
    Last modified:July 27, 2011 - v3
    Checksum:iB5A16F8BD4B80340
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 371D → G in AAB71763. (PubMed:9479488)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF010586 mRNA. Translation: AAB66331.1.
    U92565 mRNA. Translation: AAB71763.1.
    AF071549 mRNA. Translation: AAD41620.1.
    AK138214 mRNA. Translation: BAE23585.1.
    AK138472 mRNA. Translation: BAE23673.1.
    AK138574 mRNA. Translation: BAE23702.1.
    AK154646 mRNA. Translation: BAE32737.1.
    CH466525 Genomic DNA. Translation: EDL11144.1.
    BC006650 mRNA. Translation: AAH06650.1.
    BC054838 mRNA. Translation: AAH54838.1.
    CCDSiCCDS22548.1.
    RefSeqiNP_033168.2. NM_009142.3.
    UniGeneiMm.103711.

    Genome annotation databases

    EnsembliENSMUST00000034230; ENSMUSP00000034230; ENSMUSG00000031778.
    GeneIDi20312.
    KEGGimmu:20312.
    UCSCiuc009mwx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF010586 mRNA. Translation: AAB66331.1 .
    U92565 mRNA. Translation: AAB71763.1 .
    AF071549 mRNA. Translation: AAD41620.1 .
    AK138214 mRNA. Translation: BAE23585.1 .
    AK138472 mRNA. Translation: BAE23673.1 .
    AK138574 mRNA. Translation: BAE23702.1 .
    AK154646 mRNA. Translation: BAE32737.1 .
    CH466525 Genomic DNA. Translation: EDL11144.1 .
    BC006650 mRNA. Translation: AAH06650.1 .
    BC054838 mRNA. Translation: AAH54838.1 .
    CCDSi CCDS22548.1.
    RefSeqi NP_033168.2. NM_009142.3.
    UniGenei Mm.103711.

    3D structure databases

    ProteinModelPortali O35188.
    SMRi O35188. Positions 32-97.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35188. 1 interaction.
    MINTi MINT-4120736.

    PTM databases

    PhosphoSitei O35188.

    Proteomic databases

    PaxDbi O35188.
    PRIDEi O35188.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034230 ; ENSMUSP00000034230 ; ENSMUSG00000031778 .
    GeneIDi 20312.
    KEGGi mmu:20312.
    UCSCi uc009mwx.1. mouse.

    Organism-specific databases

    CTDi 6376.
    MGIi MGI:1097153. Cx3cl1.

    Phylogenomic databases

    eggNOGi NOG46337.
    GeneTreei ENSGT00510000049483.
    HOGENOMi HOG000036946.
    HOVERGENi HBG057269.
    InParanoidi Q91V44.
    KOi K05508.
    OMAi YQSLQGC.
    OrthoDBi EOG75TMD4.
    TreeFami TF337534.

    Miscellaneous databases

    NextBioi 298087.
    PMAP-CutDB Q91V44.
    PROi O35188.
    SOURCEi Search...

    Gene expression databases

    Bgeei O35188.
    CleanExi MM_CX3CL1.
    Genevestigatori O35188.

    Family and domain databases

    InterProi IPR008097. Chemokine_CX3CL1.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    PRINTSi PR01721. FRACTALKINE.
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and characterization of a new type of mouse chemokine."
      Rossi D.L., Hardiman G., Copeland N.G., Gilbert D.J., Jenkins N., Zlotnik A., Bazan J.F.
      Genomics 47:163-170(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Brain.
    3. "Fractalkine and macrophage-derived chemokine: T cell-attracting chemokines expressed in T cell area dendritic cells."
      Kanazawa N., Nakamura T., Tashiro K., Muramatsu M., Morita K., Yoneda K., Inaba K., Imamura S., Honjo T.
      Eur. J. Immunol. 29:1925-1932(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Hypothalamus and Spinal cord.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Eye and Mammary tumor.
    7. Cited for: INDUCTION.

    Entry informationi

    Entry nameiX3CL1_MOUSE
    AccessioniPrimary (citable) accession number: O35188
    Secondary accession number(s): O35933, Q91V44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3