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O35188 (X3CL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fractalkine
Alternative name(s):
C-X3-C motif chemokine 1
CX3C membrane-anchored chemokine
Neurotactin
Small-inducible cytokine D1

Cleaved into the following chain:

  1. Processed fractalkine
Gene names
Name:Cx3cl1
Synonyms:Cx3c, Fkn, Scyd1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium By similarity. Binds to CX3CR1.

Subunit structure

Monomer By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Processed fractalkine: Secreted.

Tissue specificity

Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

Post-translational modification

A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

Sequence similarities

Belongs to the intercrine delta family.

Ontologies

Keywords
   Biological processCell adhesion
Chemotaxis
   Cellular componentCell membrane
Membrane
Secreted
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionCytokine
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis involved in wound healing

Inferred from mutant phenotype PubMed 18097059. Source: MGI

cell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

cytokine-mediated signaling pathway

Inferred from mutant phenotype PubMed 18971423. Source: MGI

immune response

Inferred from electronic annotation. Source: InterPro

lymphocyte chemotaxis

Inferred from direct assay Ref.1. Source: MGI

macrophage chemotaxis

Inferred from mutant phenotype PubMed 16272359. Source: MGI

negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Inferred from direct assay PubMed 18322241PubMed 18971423. Source: MGI

neutrophil chemotaxis

Inferred from direct assay Ref.1. Source: MGI

positive regulation of angiogenesis

Inferred from mutant phenotype PubMed 17971299. Source: MGI

positive regulation of calcium-independent cell-cell adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of inflammatory response

Inferred from electronic annotation. Source: Ensembl

positive regulation of transforming growth factor beta1 production

Inferred from mutant phenotype PubMed 18097059. Source: MGI

wound healing

Inferred from mutant phenotype PubMed 18097059. Source: MGI

   Cellular_componentcell surface

Inferred from direct assay Ref.1. Source: MGI

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionchemokine activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 395371Fractalkine
PRO_0000005253
Chain25 – ?337313Processed fractalkine
PRO_0000296225

Regions

Topological domain25 – 336312Extracellular Potential
Transmembrane337 – 35721Helical; Potential
Topological domain358 – 39538Cytoplasmic Potential
Region25 – 10076Chemokine
Region101 – 336236Mucin-like stalk

Sites

Site337 – 3382Cleavage; to produce soluble form Potential

Amino acid modifications

Disulfide bond32 ↔ 58 By similarity
Disulfide bond36 ↔ 74 By similarity

Experimental info

Sequence conflict371D → G in AAB71763. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O35188 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: B5A16F8BD4B80340

FASTA39542,099
        10         20         30         40         50         60 
MAPSPLAWLL RLAAFFHLCT LLPGQHLGMT KCEIMCDKMT SRIPVALLIR YQLNQESCGK 

        70         80         90        100        110        120 
RAIVLETTQH RRFCADPKEK WVQDAMKHLD HQAAALTKNG GKFEKRVDNV TPGITLATRG 

       130        140        150        160        170        180 
LSPSALTKPE SATLEDLALE LTTISQEARG TMGTSQEPPA AVTGSSLSTS EAQDAGLTAK 

       190        200        210        220        230        240 
PQSIGSFEAA DISTTVWPSP AVYQSGSSSW AEEKATESPS TTAPSPQVST TSPSTPEENV 

       250        260        270        280        290        300 
GSEGQPPWVQ GQDLSPEKSL GSEEINPVHT DNFQERGPGN TVHPSVAPIS SEETPSPELV 

       310        320        330        340        350        360 
ASGSQAPKIE EPIHATADPQ KLSVLITPVP DTQAATRRQA VGLLAFLGLL FCLGVAMFAY 

       370        380        390 
QSLQGCPRKM AGEMVEGLRY VPRSCGSNSY VLVPV 

« Hide

References

« Hide 'large scale' references
[1]"Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation."
Pan Y., Lloyd C., Zhou H., Dolich S., Deeds J., Gonzalo J.-A., Vath J., Gosselin M., Ma J., Dussault B., Woolf E., Alperin G., Culpepper J., Gutierrez-Ramos J.-C., Gearing D.P.
Nature 387:611-617(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and characterization of a new type of mouse chemokine."
Rossi D.L., Hardiman G., Copeland N.G., Gilbert D.J., Jenkins N., Zlotnik A., Bazan J.F.
Genomics 47:163-170(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Brain.
[3]"Fractalkine and macrophage-derived chemokine: T cell-attracting chemokines expressed in T cell area dendritic cells."
Kanazawa N., Nakamura T., Tashiro K., Muramatsu M., Morita K., Yoneda K., Inaba K., Imamura S., Honjo T.
Eur. J. Immunol. 29:1925-1932(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Hypothalamus and Spinal cord.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Eye and Mammary tumor.
[7]"Neuronal expression of fractalkine in the presence and absence of inflammation."
Schwaeble W.J., Stover C.M., Schall T.J., Dairaghi D.J., Trinder P.K.E., Linington C., Iglesias A., Schubart A., Lynch N.J., Weihe E., Schaefer M.K.-H.
FEBS Lett. 439:203-207(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF010586 mRNA. Translation: AAB66331.1.
U92565 mRNA. Translation: AAB71763.1.
AF071549 mRNA. Translation: AAD41620.1.
AK138214 mRNA. Translation: BAE23585.1.
AK138472 mRNA. Translation: BAE23673.1.
AK138574 mRNA. Translation: BAE23702.1.
AK154646 mRNA. Translation: BAE32737.1.
CH466525 Genomic DNA. Translation: EDL11144.1.
BC006650 mRNA. Translation: AAH06650.1.
BC054838 mRNA. Translation: AAH54838.1.
RefSeqNP_033168.2. NM_009142.3.
UniGeneMm.103711.

3D structure databases

ProteinModelPortalO35188.
SMRO35188. Positions 32-97.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35188. 1 interaction.
MINTMINT-4120736.

PTM databases

PhosphoSiteO35188.

Proteomic databases

PaxDbO35188.
PRIDEO35188.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034230; ENSMUSP00000034230; ENSMUSG00000031778.
GeneID20312.
KEGGmmu:20312.
UCSCuc009mwx.1. mouse.

Organism-specific databases

CTD6376.
MGIMGI:1097153. Cx3cl1.

Phylogenomic databases

eggNOGNOG46337.
GeneTreeENSGT00510000049483.
HOGENOMHOG000036946.
HOVERGENHBG057269.
InParanoidQ91V44.
KOK05508.
OMAYQSLQGC.
OrthoDBEOG75TMD4.
TreeFamTF337534.

Gene expression databases

BgeeO35188.
CleanExMM_CX3CL1.
GenevestigatorO35188.

Family and domain databases

InterProIPR008097. Chemokine_CX3CL1.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
PRINTSPR01721. FRACTALKINE.
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
ProtoNetSearch...

Other

NextBio298087.
PMAP-CutDBQ91V44.
PROO35188.
SOURCESearch...

Entry information

Entry nameX3CL1_MOUSE
AccessionPrimary (citable) accession number: O35188
Secondary accession number(s): O35933, Q91V44
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot