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O35188

- X3CL1_MOUSE

UniProt

O35188 - X3CL1_MOUSE

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Protein
Fractalkine
Gene
Cx3cl1, Cx3c, Fkn, Scyd1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium By similarity. Binds to CX3CR1.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei337 – 3382Cleavage; to produce soluble form Reviewed prediction

GO - Molecular functioni

  1. chemokine activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. angiogenesis involved in wound healing Source: MGI
  2. cell adhesion Source: UniProtKB-KW
  3. chemotaxis Source: UniProtKB
  4. cytokine-mediated signaling pathway Source: MGI
  5. immune response Source: InterPro
  6. lymphocyte chemotaxis Source: MGI
  7. macrophage chemotaxis Source: MGI
  8. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  9. neutrophil chemotaxis Source: MGI
  10. positive regulation of angiogenesis Source: MGI
  11. positive regulation of calcium-independent cell-cell adhesion Source: Ensembl
  12. positive regulation of inflammatory response Source: Ensembl
  13. positive regulation of transforming growth factor beta1 production Source: MGI
  14. wound healing Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Cell adhesion, Chemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Fractalkine
Alternative name(s):
C-X3-C motif chemokine 1
CX3C membrane-anchored chemokine
Neurotactin
Small-inducible cytokine D1
Cleaved into the following chain:
Gene namesi
Name:Cx3cl1
Synonyms:Cx3c, Fkn, Scyd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1097153. Cx3cl1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 336312Extracellular Reviewed prediction
Add
BLAST
Transmembranei337 – 35721Helical; Reviewed prediction
Add
BLAST
Topological domaini358 – 39538Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. extracellular space Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Chaini25 – 395371Fractalkine
PRO_0000005253Add
BLAST
Chaini25 – ?337313Processed fractalkine
PRO_0000296225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 58 By similarity
Disulfide bondi36 ↔ 74 By similarity

Post-translational modificationi

A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO35188.
PRIDEiO35188.

PTM databases

PhosphoSiteiO35188.

Miscellaneous databases

PMAP-CutDBiQ91V44.

Expressioni

Tissue specificityi

Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

Gene expression databases

BgeeiO35188.
CleanExiMM_CX3CL1.
GenevestigatoriO35188.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

IntActiO35188. 1 interaction.
MINTiMINT-4120736.

Structurei

3D structure databases

ProteinModelPortaliO35188.
SMRiO35188. Positions 32-97.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 10076Chemokine
Add
BLAST
Regioni101 – 336236Mucin-like stalk
Add
BLAST

Sequence similaritiesi

Belongs to the intercrine delta family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46337.
GeneTreeiENSGT00510000049483.
HOGENOMiHOG000036946.
HOVERGENiHBG057269.
InParanoidiQ91V44.
KOiK05508.
OMAiYQSLQGC.
OrthoDBiEOG75TMD4.
TreeFamiTF337534.

Family and domain databases

InterProiIPR008097. Chemokine_CX3CL1.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR01721. FRACTALKINE.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35188-1 [UniParc]FASTAAdd to Basket

« Hide

MAPSPLAWLL RLAAFFHLCT LLPGQHLGMT KCEIMCDKMT SRIPVALLIR    50
YQLNQESCGK RAIVLETTQH RRFCADPKEK WVQDAMKHLD HQAAALTKNG 100
GKFEKRVDNV TPGITLATRG LSPSALTKPE SATLEDLALE LTTISQEARG 150
TMGTSQEPPA AVTGSSLSTS EAQDAGLTAK PQSIGSFEAA DISTTVWPSP 200
AVYQSGSSSW AEEKATESPS TTAPSPQVST TSPSTPEENV GSEGQPPWVQ 250
GQDLSPEKSL GSEEINPVHT DNFQERGPGN TVHPSVAPIS SEETPSPELV 300
ASGSQAPKIE EPIHATADPQ KLSVLITPVP DTQAATRRQA VGLLAFLGLL 350
FCLGVAMFAY QSLQGCPRKM AGEMVEGLRY VPRSCGSNSY VLVPV 395
Length:395
Mass (Da):42,099
Last modified:July 27, 2011 - v3
Checksum:iB5A16F8BD4B80340
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371D → G in AAB71763. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF010586 mRNA. Translation: AAB66331.1.
U92565 mRNA. Translation: AAB71763.1.
AF071549 mRNA. Translation: AAD41620.1.
AK138214 mRNA. Translation: BAE23585.1.
AK138472 mRNA. Translation: BAE23673.1.
AK138574 mRNA. Translation: BAE23702.1.
AK154646 mRNA. Translation: BAE32737.1.
CH466525 Genomic DNA. Translation: EDL11144.1.
BC006650 mRNA. Translation: AAH06650.1.
BC054838 mRNA. Translation: AAH54838.1.
CCDSiCCDS22548.1.
RefSeqiNP_033168.2. NM_009142.3.
UniGeneiMm.103711.

Genome annotation databases

EnsembliENSMUST00000034230; ENSMUSP00000034230; ENSMUSG00000031778.
GeneIDi20312.
KEGGimmu:20312.
UCSCiuc009mwx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF010586 mRNA. Translation: AAB66331.1 .
U92565 mRNA. Translation: AAB71763.1 .
AF071549 mRNA. Translation: AAD41620.1 .
AK138214 mRNA. Translation: BAE23585.1 .
AK138472 mRNA. Translation: BAE23673.1 .
AK138574 mRNA. Translation: BAE23702.1 .
AK154646 mRNA. Translation: BAE32737.1 .
CH466525 Genomic DNA. Translation: EDL11144.1 .
BC006650 mRNA. Translation: AAH06650.1 .
BC054838 mRNA. Translation: AAH54838.1 .
CCDSi CCDS22548.1.
RefSeqi NP_033168.2. NM_009142.3.
UniGenei Mm.103711.

3D structure databases

ProteinModelPortali O35188.
SMRi O35188. Positions 32-97.
ModBasei Search...

Protein-protein interaction databases

IntActi O35188. 1 interaction.
MINTi MINT-4120736.

PTM databases

PhosphoSitei O35188.

Proteomic databases

PaxDbi O35188.
PRIDEi O35188.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034230 ; ENSMUSP00000034230 ; ENSMUSG00000031778 .
GeneIDi 20312.
KEGGi mmu:20312.
UCSCi uc009mwx.1. mouse.

Organism-specific databases

CTDi 6376.
MGIi MGI:1097153. Cx3cl1.

Phylogenomic databases

eggNOGi NOG46337.
GeneTreei ENSGT00510000049483.
HOGENOMi HOG000036946.
HOVERGENi HBG057269.
InParanoidi Q91V44.
KOi K05508.
OMAi YQSLQGC.
OrthoDBi EOG75TMD4.
TreeFami TF337534.

Miscellaneous databases

NextBioi 298087.
PMAP-CutDBi Q91V44.
PROi O35188.
SOURCEi Search...

Gene expression databases

Bgeei O35188.
CleanExi MM_CX3CL1.
Genevestigatori O35188.

Family and domain databases

InterProi IPR008097. Chemokine_CX3CL1.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view ]
Pfami PF00048. IL8. 1 hit.
[Graphical view ]
PRINTSi PR01721. FRACTALKINE.
SMARTi SM00199. SCY. 1 hit.
[Graphical view ]
SUPFAMi SSF54117. SSF54117. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of a new type of mouse chemokine."
    Rossi D.L., Hardiman G., Copeland N.G., Gilbert D.J., Jenkins N., Zlotnik A., Bazan J.F.
    Genomics 47:163-170(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Brain.
  3. "Fractalkine and macrophage-derived chemokine: T cell-attracting chemokines expressed in T cell area dendritic cells."
    Kanazawa N., Nakamura T., Tashiro K., Muramatsu M., Morita K., Yoneda K., Inaba K., Imamura S., Honjo T.
    Eur. J. Immunol. 29:1925-1932(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Hypothalamus and Spinal cord.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Eye and Mammary tumor.
  7. Cited for: INDUCTION.

Entry informationi

Entry nameiX3CL1_MOUSE
AccessioniPrimary (citable) accession number: O35188
Secondary accession number(s): O35933, Q91V44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi