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Protein

Cathepsin K

Gene

Ctsk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation (By similarity).By similarity

Catalytic activityi

Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei139 – 1391By similarity
Active sitei276 – 2761By similarity
Active sitei296 – 2961By similarity

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: GO_Central
  • cysteine-type peptidase activity Source: RGD

GO - Biological processi

  • bone resorption Source: RGD
  • collagen catabolic process Source: Ensembl
  • intramembranous ossification Source: RGD
  • positive regulation of protein targeting to mitochondrion Source: Ensembl
  • proteolysis Source: RGD
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
  • regulation of mitophagy Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.38. 5301.
ReactomeiR-RNO-1442490. Collagen degradation.
R-RNO-1474228. Degradation of the extracellular matrix.
R-RNO-1592389. Activation of Matrix Metalloproteinases.
R-RNO-1679131. Trafficking and processing of endosomal TLR.

Protein family/group databases

MEROPSiI29.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin K (EC:3.4.22.38)
Gene namesi
Name:Ctsk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi61810. Ctsk.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: RGD
  • lysosome Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3034.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence analysisAdd
BLAST
Propeptidei16 – 11499Activation peptidePRO_0000026307Add
BLAST
Chaini115 – 329215Cathepsin KPRO_0000026308Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence analysis
Disulfide bondi136 ↔ 177By similarity
Disulfide bondi170 ↔ 210By similarity
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence analysis
Disulfide bondi269 ↔ 318By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO35186.
PRIDEiO35186.

PTM databases

iPTMnetiO35186.
PhosphoSiteiO35186.

Expressioni

Gene expression databases

BgeeiENSRNOG00000021155.
GenevisibleiO35186. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028730.

Chemistry

BindingDBiO35186.

Structurei

3D structure databases

ProteinModelPortaliO35186.
SMRiO35186. Positions 16-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiO35186.
KOiK01371.
OMAiKHISIHN.
OrthoDBiEOG091G0AKT.
PhylomeDBiO35186.
TreeFamiTF313739.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR015644. Peptidase_C1A_cathepsin-K.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PTHR12411:SF55. PTHR12411:SF55. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWVFKFLLLP VVSFALSPEE TLDTQWELWK KTHGKQYNSK VDEISRRLIW
60 70 80 90 100
EKNLKKISVH NLEASLGAHT YELAMNHLGD MTSEEVVQKM TGLRVPPSRS
110 120 130 140 150
FSNDTLYTPE WEGRVPDSID YRKKGYVTPV KNQGQCGSCW AFSSAGALEG
160 170 180 190 200
QLKKKTGKLL ALSPQNLVDC VSENYGCGGG YMTTAFQYVQ QNGGIDSEDA
210 220 230 240 250
YPYVGQDESC MYNATAKAAK CRGYREIPVG NEKALKRAVA RVGPVSVSID
260 270 280 290 300
ASLTSFQFYS RGVYYDENCD RDNVNHAVLV VGYGTQKGNK YWIIKNSWGE
310 320
SWGNKGYVLL ARNKNNACGI TNLASFPKM
Length:329
Mass (Da):36,791
Last modified:January 1, 1998 - v1
Checksum:iB416008509CF606A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010306 mRNA. Translation: AAB65743.1.
BC078793 mRNA. Translation: AAH78793.1.
RefSeqiNP_113748.1. NM_031560.2.
UniGeneiRn.5598.

Genome annotation databases

EnsembliENSRNOT00000028730; ENSRNOP00000028730; ENSRNOG00000021155.
GeneIDi29175.
KEGGirno:29175.
UCSCiRGD:61810. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010306 mRNA. Translation: AAB65743.1.
BC078793 mRNA. Translation: AAH78793.1.
RefSeqiNP_113748.1. NM_031560.2.
UniGeneiRn.5598.

3D structure databases

ProteinModelPortaliO35186.
SMRiO35186. Positions 16-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028730.

Chemistry

BindingDBiO35186.
ChEMBLiCHEMBL3034.

Protein family/group databases

MEROPSiI29.007.

PTM databases

iPTMnetiO35186.
PhosphoSiteiO35186.

Proteomic databases

PaxDbiO35186.
PRIDEiO35186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028730; ENSRNOP00000028730; ENSRNOG00000021155.
GeneIDi29175.
KEGGirno:29175.
UCSCiRGD:61810. rat.

Organism-specific databases

CTDi1513.
RGDi61810. Ctsk.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiO35186.
KOiK01371.
OMAiKHISIHN.
OrthoDBiEOG091G0AKT.
PhylomeDBiO35186.
TreeFamiTF313739.

Enzyme and pathway databases

BRENDAi3.4.22.38. 5301.
ReactomeiR-RNO-1442490. Collagen degradation.
R-RNO-1474228. Degradation of the extracellular matrix.
R-RNO-1592389. Activation of Matrix Metalloproteinases.
R-RNO-1679131. Trafficking and processing of endosomal TLR.

Miscellaneous databases

PROiO35186.

Gene expression databases

BgeeiENSRNOG00000021155.
GenevisibleiO35186. RN.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR015644. Peptidase_C1A_cathepsin-K.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PTHR12411:SF55. PTHR12411:SF55. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCATK_RAT
AccessioniPrimary (citable) accession number: O35186
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.