O35186 (CATK_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cathepsin K
EC=3.4.22.38

Gene names

Name:

Ctsk

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation By similarity.
Catalytic activity	Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bone resorption Inferred from mutant phenotype PubMed 10430032. Source: RGD intramembranous ossification Inferred from expression pattern PubMed 16193234. Source: RGD proteolysis Traceable author statement PubMed 14585819PubMed 15312243. Source: RGD
Cellular_component	extracellular space Inferred from direct assay PubMed 16261450. Source: RGD lysosome Inferred from direct assay PubMed 14585819. Source: RGD
Molecular_function	cysteine-type peptidase activity Traceable author statement PubMed 14585819. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Propeptide	16 – 114	99	Activation peptide		PRO_0000026307
	Chain	115 – 329	215	Cathepsin K		PRO_0000026308
Sites
	Active site	139	1	By similarity
	Active site	276	1	By similarity
	Active site	296	1	By similarity
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Disulfide bond	136 ↔ 177		By similarity
	Disulfide bond	170 ↔ 210		By similarity
	Disulfide bond	269 ↔ 318		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35186 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B416008509CF606A	FASTA	329	36,791
	10 20 30 40 50 60 MWVFKFLLLP VVSFALSPEE TLDTQWELWK KTHGKQYNSK VDEISRRLIW EKNLKKISVH 70 80 90 100 110 120 NLEASLGAHT YELAMNHLGD MTSEEVVQKM TGLRVPPSRS FSNDTLYTPE WEGRVPDSID 130 140 150 160 170 180 YRKKGYVTPV KNQGQCGSCW AFSSAGALEG QLKKKTGKLL ALSPQNLVDC VSENYGCGGG 190 200 210 220 230 240 YMTTAFQYVQ QNGGIDSEDA YPYVGQDESC MYNATAKAAK CRGYREIPVG NEKALKRAVA 250 260 270 280 290 300 RVGPVSVSID ASLTSFQFYS RGVYYDENCD RDNVNHAVLV VGYGTQKGNK YWIIKNSWGE 310 320 SWGNKGYVLL ARNKNNACGI TNLASFPKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Payne J.A., Miller L.R. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Bone.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF010306 mRNA. Translation: AAB65743.1. BC078793 mRNA. Translation: AAH78793.1.
IPI	IPI00206378.
RefSeq	NP_113748.1. NM_031560.2.
UniGene	Rn.5598.
3D structure databases
ProteinModelPortal	O35186.
SMR	O35186. Positions 16-329.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000028730.
Protein family/group databases
MEROPS	I29.007.
PTM databases
PhosphoSite	O35186.
Proteomic databases
PaxDb	O35186.
PRIDE	O35186.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028730; ENSRNOP00000028730; ENSRNOG00000021155.
GeneID	29175.
KEGG	rno:29175.
UCSC	RGD:61810. rat.
Organism-specific databases
CTD	1513.
RGD	61810. Ctsk.
Phylogenomic databases
eggNOG	COG4870.
GeneTree	ENSGT00560000076577.
HOGENOM	HOG000230774.
HOVERGEN	HBG011513.
InParanoid	O35186.
KO	K01371.
OMA	YKEIPEG.
OrthoDB	EOG4SJ5FC.
Gene expression databases
Genevestigator	O35186.
GermOnline	ENSRNOG00000021155. Rattus norvegicus.
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR015644. Peptidase_C1A_cathepsin-K. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit. PTHR12411:SF55. PTHR12411:SF55. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O35186.
ChEMBL	CHEMBL3034.
NextBio	608248.

Entry information

Entry name

CATK_RAT

Accession

Primary (citable) accession number: O35186

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program