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O35181 (NRG3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pro-neuregulin-3, membrane-bound isoform

Short name=Pro-NRG3

Cleaved into the following chain:

  1. Neuregulin-3
    Short name=NRG-3
Gene names
Name:Nrg3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length713 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. Ref.1

Subunit structure

Interacts with ERBB4. Ref.1

Subcellular location

Pro-neuregulin-3, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein By similarity. Note: Does not seem to be active By similarity.

Neuregulin-3: Secreted By similarity.

Tissue specificity

Expressed in sympathetic, motor, and sensory neurons.

Developmental stage

Detected as early as 11 dpc. At 13 dpc detected mainly in the nervous system. At 16 dpc, detected in the brain, spinal cord, trigeminal, vestibular-cochlear, and spinal ganglia. In adults, expressed in spinal cord, and numerous brain regions.

Domain

The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization By similarity.

ERBB receptor binding is elicited entirely by the EGF-like domain By similarity.

Post-translational modification

Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form By similarity.

Extensive glycosylation precedes the proteolytic cleavage By similarity.

Sequence similarities

Belongs to the neuregulin family.

Contains 1 EGF-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 713713Pro-neuregulin-3, membrane-bound isoform
PRO_0000019483
Chain1 – 361361Neuregulin-3
PRO_0000019484

Regions

Topological domain1 – 362362Extracellular Potential
Transmembrane363 – 38321Helical; Note=Internal signal sequence; Potential
Topological domain384 – 713330Cytoplasmic Potential
Domain288 – 33144EGF-like
Compositional bias13 – 219Poly-Ala
Compositional bias26 – 349Poly-Ala
Compositional bias105 – 287183Ser/Thr-rich
Compositional bias127 – 1359Poly-Thr
Compositional bias250 – 2534Poly-Ala
Compositional bias254 – 26310Poly-Ser
Compositional bias264 – 2674Poly-Thr

Amino acid modifications

Disulfide bond292 ↔ 306 By similarity
Disulfide bond300 ↔ 319 By similarity
Disulfide bond321 ↔ 330 By similarity

Sequences

Sequence LengthMass (Da)Tools
O35181 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 9F7D1D5E7FC8DCF0

FASTA71377,370
        10         20         30         40         50         60 
MSEGAAGASP PGAASAAAAS AEEGTAAAAA AAAAGGGPDG GGEGAAEPPR ELRCSDCIVW 

        70         80         90        100        110        120 
NRQQTWLCVV PLFIGFIGLG LSLMLLKWIV VGSVKEYVPT DLVDSKGMGQ DPFFLSKPSS 

       130        140        150        160        170        180 
FPKAMETTTT TTSTTSPATP SAGGAASSRT PNRISTRLTT ITRAPTRFPG HRVPIRASPR 

       190        200        210        220        230        240 
STTARNTAAP PTVLSTTAPF FSSSTPGSRP PMPGAPSTQA MPSWPTAAYA TSSYLHDSTP 

       250        260        270        280        290        300 
SWTLSPFQDA AAASSSSPSS TSSTTTTPET STSPKFHTTT YSTERSEHFK PCRDKDLAYC 

       310        320        330        340        350        360 
LNDGECFVIE TLTGSHKHCR CKEGYQGVRC DQFLPKTDSI LSDPTDHLGI EFMESEDVYQ 

       370        380        390        400        410        420 
RQVLSISCII FGIVIVGMFC AAFYFKSKKQ AKQIQEHLKE SQNGKNYSLK ASSTKSESLM 

       430        440        450        460        470        480 
KSHVHLQNYS KADRHPVTAL EKIMESSFSA PQSFPEVTSP DRGSQPIKHH SPGQRSGMLH 

       490        500        510        520        530        540 
RNTFRRAPPS PRSRLGGIVG PAYQQLEESR IPDQDTIPCQ GIEVRKTISH LPIQLWCVER 

       550        560        570        580        590        600 
PLDLKYVSNG LRTQQNASIN MQLPSRETNP YFNSLDQKDL VGYLSPRANS VPIIPSMGLE 

       610        620        630        640        650        660 
ETCMQMPGIS DVKSIKWCKN SYSADIVNAS MPVSDCLLEE QQEVKILLET VQEQIRILTD 

       670        680        690        700        710 
ARRSEDFELA SMETEDSASE NTAFLPLSPT AKSEREAQFV LRNEIQRDSV LTK 

« Hide

References

[1]"Neuregulin-3 (NRG3): a novel neural tissue-enriched protein that binds and activates ErbB4."
Zhang D., Sliwkowski M.X., Mark M., Frantz G., Akita R., Sun Y., Hillan K., Crowley C., Brush J., Godowski P.J.
Proc. Natl. Acad. Sci. U.S.A. 94:9562-9567(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ERBB4.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF010130 mRNA. Translation: AAB70914.1.
PIRT44447.
RefSeqNP_032760.1. NM_008734.3.
UniGeneMm.258705.

3D structure databases

ProteinModelPortalO35181.
SMRO35181. Positions 292-333.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO35181.

Proteomic databases

PRIDEO35181.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000166968; ENSMUSP00000136884; ENSMUSG00000041014.
GeneID18183.
KEGGmmu:18183.
UCSCuc007tbz.2. mouse.

Organism-specific databases

CTD10718.
MGIMGI:1097165. Nrg3.

Phylogenomic databases

eggNOGNOG40523.
GeneTreeENSGT00710000106660.
HOVERGENHBG006532.
InParanoidO35181.
KOK05457.
OMAESSFTGP.
OrthoDBEOG7SR4KZ.
PhylomeDBO35181.
TreeFamTF336537.

Gene expression databases

BgeeO35181.
CleanExMM_NRG3.
GenevestigatorO35181.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
[Graphical view]
SMARTSM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293498.
PROO35181.
SOURCESearch...

Entry information

Entry nameNRG3_MOUSE
AccessionPrimary (citable) accession number: O35181
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot