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Protein

Endophilin-A3

Gene

Sh3gl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in endocytosis. May recruit other proteins to membranes with high curvature.1 Publication

Miscellaneous

Cells overexpressing Sh3gl3 show inhibition of transferrin uptake. Olfactory epithelium-derived cells overexpressing SH3GL3, DRD2 and GRK2 show accelerated differentiation upon addition of apomorphine due to reduced agonist-induced endocytosis of DRD2.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endophilin-A3
Alternative name(s):
Endophilin-3
SH3 domain protein 2C
SH3 domain-containing GRB2-like protein 3
SH3p13
Gene namesi
Name:Sh3gl3
Synonyms:Sh3d2c1, Sh3p13
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620578. Sh3gl3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467521 – 347Endophilin-A3Add BLAST347

Proteomic databases

PaxDbiO35180.
PRIDEiO35180.

PTM databases

iPTMnetiO35180.
PhosphoSitePlusiO35180.

Expressioni

Tissue specificityi

Expressed at high level in testis and at lower level in brain and liver.2 Publications

Gene expression databases

BgeeiENSRNOG00000019776.
ExpressionAtlasiO35180. baseline and differential.
GenevisibleiO35180. RN.

Interactioni

Subunit structurei

Interacts with SGIP1 and DYDC1 (By similarity). Interacts with FASLG. Interacts with ATX2. Interacts with BIN2 (By similarity). Interacts with ARC, DNM1 and SYNJ1.By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

ELMiO35180.
IntActiO35180. 3 interactors.
MINTiMINT-1526354.
STRINGi10116.ENSRNOP00000026870.

Structurei

3D structure databases

ProteinModelPortaliO35180.
SMRiO35180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 249BARPROSITE-ProRule annotationAdd BLAST232
Domaini285 – 344SH3PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 21Membrane-binding amphipathic helixBy similarityAdd BLAST21
Regioni60 – 87Required for dimerization upon membrane associationBy similarityAdd BLAST28
Regioni218 – 254Interaction with ARCBy similarityAdd BLAST37

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili180 – 201Sequence analysisAdd BLAST22

Domaini

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.By similarity

Sequence similaritiesi

Belongs to the endophilin family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG1118. Eukaryota.
ENOG410XNYB. LUCA.
GeneTreeiENSGT00550000074464.
HOGENOMiHOG000231641.
HOVERGENiHBG052866.
InParanoidiO35180.
OMAiNVPMDQP.
OrthoDBiEOG091G16FW.
PhylomeDBiO35180.

Family and domain databases

CDDicd07615. BAR_Endophilin_A3. 1 hit.
cd11803. SH3_Endophilin_A. 1 hit.
InterProiView protein in InterPro
IPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR028501. Endophilin-A.
IPR032469. Endophilin-A3_BAR.
IPR035824. Endophilin_A_SH3.
IPR011511. SH3_2.
IPR001452. SH3_domain.
PANTHERiPTHR10663:SF260. PTHR10663:SF260. 1 hit.
PfamiView protein in Pfam
PF03114. BAR. 1 hit.
PF07653. SH3_2. 1 hit.
PRINTSiPR00452. SH3DOMAIN.
SMARTiView protein in SMART
SM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
SUPFAMiSSF103657. SSF103657. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiView protein in PROSITE
PS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.

Sequencei

Sequence statusi: Complete.

O35180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAGLKKQF HKASQLFSEK ISGAEGTKLD EEFLDMEKKI DITSKAVAEI
60 70 80 90 100
LSKATEYLQP NPAYRAKLGM LNTMSKLRGQ VKATGYPQTE GLLGDCMLKY
110 120 130 140 150
GRELGEDSAF GNSLVEVGEA LKLMAEVKDS LDINVKQTFI DPLQLLQDKD
160 170 180 190 200
LKEIGHHLRK LEGRRLDYDY KKKRVGKIPE EEIRQAVEKF EESKELAERS
210 220 230 240 250
MFNFLENDVE QVSQLAVFVE AALDYHRQST EILQELQNKL ELRIALASQV
260 270 280 290 300
PRRDYMPKPV NTSSTNANGV EPSSSSKLTG TDIPSDQPCC RGLYDFEPEN
310 320 330 340
EGELGFKEGD IITLTNQIDE NWYEGMLRGE SGFFPINYVE VIVPLPR
Length:347
Mass (Da):39,166
Last modified:October 23, 2007 - v2
Checksum:iD229D63BC7DA3733
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107D → H in AAC14884 (PubMed:9238017).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC128742 Genomic DNA. No translation available.
AF009604 mRNA. Translation: AAC14884.1.
AB008159 mRNA. Translation: BAA22920.2.
RefSeqiXP_006229542.1. XM_006229480.3.
XP_006229547.1. XM_006229485.1.
UniGeneiRn.5909.

Genome annotation databases

EnsembliENSRNOT00000079989; ENSRNOP00000072673; ENSRNOG00000019776.
GeneIDi81921.

Similar proteinsi

Entry informationi

Entry nameiSH3G3_RAT
AccessioniPrimary (citable) accession number: O35180
Secondary accession number(s): O35104
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 23, 2007
Last modified: September 27, 2017
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families