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Protein

Endophilin-A1

Gene

Sh3gl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes (By similarity).By similarity2 Publications

Miscellaneous

The N-BAR domain binds liposomes and mediates dimerization of BAR domains upon liposome binding. It induces formation of tubules from liposomes as well as fusion of liposome tubules. Cells overexpressing Sh3gl2 show no effect on transferrin uptake.

GO - Molecular functioni

  • identical protein binding Source: RGD
  • lipid binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein kinase binding Source: RGD

GO - Biological processi

  • cellular response to brain-derived neurotrophic factor stimulus Source: UniProtKB
  • dendrite extension Source: UniProtKB
  • membrane bending Source: RGD
  • membrane tubulation Source: RGD
  • neuron projection development Source: UniProtKB
  • regulation of receptor internalization Source: RGD
  • synaptic vesicle endocytosis Source: RGD
  • synaptic vesicle uncoating Source: RGD
  • vesicle scission Source: RGD

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-RNO-177504 Retrograde neurotrophin signalling
R-RNO-182971 EGFR downregulation
R-RNO-2132295 MHC class II antigen presentation
R-RNO-432720 Lysosome Vesicle Biogenesis
R-RNO-432722 Golgi Associated Vesicle Biogenesis
R-RNO-437239 Recycling pathway of L1
R-RNO-6807004 Negative regulation of MET activity
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Endophilin-A1
Alternative name(s):
Endophilin-1
SH3 domain protein 2A
SH3 domain-containing GRB2-like protein 2
SH3p4
Gene namesi
Name:Sh3gl2
Synonyms:Sh3d2a, Sh3p4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620276 Sh3gl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi263L → P: Confers inhibition of transferrin uptake comparable to Sh3gl3 upon overexpression. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467491 – 352Endophilin-A1Add BLAST352

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei262PhosphoserineBy similarity1
Modified residuei299PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35179
PRIDEiO35179

PTM databases

iPTMnetiO35179
PhosphoSitePlusiO35179

Expressioni

Tissue specificityi

Brain. Expressed at low level in the kidney.1 Publication

Gene expression databases

BgeeiENSRNOG00000006761
GenevisibleiO35179 RN

Interactioni

Subunit structurei

Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity). Interacts with OPHN1 (By similarity). Interacts with SYNJ1 and DNM1. Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation. Interacts with PDCD6IP. Interacts with ATX2. Interacts with ADAM9 and ADAM15 cytoplasmic tails. Interacts with BIN2. Interacts with TMEM108 (By similarity). Interacts with ADGRB2 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein kinase binding Source: RGD

Protein-protein interaction databases

BioGridi2505992 interactors.
CORUMiO35179
ELMiO35179
IntActiO35179 12 interactors.
MINTiO35179
STRINGi10116.ENSRNOP00000008999

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 58Combined sources28
Helixi62 – 64Combined sources3
Helixi89 – 104Combined sources16
Beta strandi106 – 109Combined sources4
Helixi110 – 138Combined sources29
Helixi140 – 173Combined sources34
Turni174 – 177Combined sources4
Helixi180 – 205Combined sources26
Helixi208 – 245Combined sources38
Beta strandi294 – 299Combined sources6
Beta strandi316 – 322Combined sources7
Beta strandi324 – 332Combined sources9
Beta strandi335 – 340Combined sources6
Helixi341 – 343Combined sources3
Beta strandi344 – 348Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C08X-ray2.90A87-247[»]
2KNBNMR-B291-352[»]
3IQLX-ray1.40A/B291-352[»]
ProteinModelPortaliO35179
SMRiO35179
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35179

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 249BARPROSITE-ProRule annotationAdd BLAST232
Domaini290 – 349SH3PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 125Binds and tubulates liposomesAdd BLAST125
Regioni1 – 21Membrane-binding amphipathic helixAdd BLAST21
Regioni60 – 87Required for dimerization upon membrane associationAdd BLAST28

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili181 – 250Sequence analysisAdd BLAST70

Domaini

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.3 Publications

Sequence similaritiesi

Belongs to the endophilin family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG1118 Eukaryota
ENOG410XNYB LUCA
GeneTreeiENSGT00550000074464
HOGENOMiHOG000231641
HOVERGENiHBG052866
InParanoidiO35179
KOiK11247
OMAiSESHNGG
OrthoDBiEOG091G16FW
PhylomeDBiO35179

Family and domain databases

CDDicd11803 SH3_Endophilin_A, 1 hit
Gene3Di1.20.1270.601 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR004148 BAR_dom
IPR028501 Endophilin-A
IPR035824 Endophilin_A_SH3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43965 PTHR43965, 1 hit
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

O35179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI
60 70 80 90 100
MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF
110 120 130 140 150
GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD
160 170 180 190 200
LREIQHHLKK LEGRRLDFDY KKKRQGKIPD EELRQALEKF DESKEIAESS
210 220 230 240 250
MFNLLEMDIE QVSQLSALVQ AQLEYHKQAV QILQQVTVRL EERIRQASSQ
260 270 280 290 300
PRREYQPKPR MSLEFATGDG TQPNGGLSHT GTPKPAGVQM DQPCCRALYD
310 320 330 340 350
FEPENEGELG FKEGDIITLT NQIDENWYEG MLHGQSGFFP INYVEILVAL

PH
Length:352
Mass (Da):39,899
Last modified:October 23, 2007 - v2
Checksum:i382B6F651885B679
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78R → H in AI044966 (PubMed:8889548).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AI044966 mRNA No translation available.
AC112121 mRNA No translation available.
AF009603 mRNA Translation: AAC14883.1
RefSeqiNP_446387.1, NM_053935.1
UniGeneiRn.10787

Genome annotation databases

EnsembliENSRNOT00000008999; ENSRNOP00000008999; ENSRNOG00000006761
GeneIDi116743
KEGGirno:116743
UCSCiRGD:620276 rat

Similar proteinsi

Entry informationi

Entry nameiSH3G2_RAT
AccessioniPrimary (citable) accession number: O35179
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 23, 2007
Last modified: March 28, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome