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Protein

Enhancer of filamentation 1

Gene

Nedd9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processCell adhesion, Cell cycle, Cell division, Growth regulation, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Enhancer of filamentation 1
Short name:
mEF1
Alternative name(s):
CRK-associated substrate-related protein
Short name:
CAS-L
Neural precursor cell expressed developmentally down-regulated protein 9
Short name:
NEDD-9
p105
Gene namesi
Name:Nedd9
Synonyms:Casl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:97302 Nedd9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000893291 – 833Enhancer of filamentation 1Add BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295PhosphoserineCombined sources1
Modified residuei368PhosphoserineBy similarity1

Post-translational modificationi

PTK2/FAK1 phosphorylates the protein at the YDYVHL motif (conserved among all cas proteins). The SRC family kinases (FYN, SRC, LCK and CRK) are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Ligation of either integrin beta-1 or B-cell antigen receptor on tonsillar B-cells and B-cell lines promotes tyrosine phosphorylation and both integrin and BCR-mediated tyrosine phosphorylation requires an intact actin network. In fibroblasts transformation with oncogene v-ABL results in an increase in tyrosine phosphorylation. Transiently phosphorylated following CD3 cross-linking and this phosphorylated form binds to CRK and C3G. A mutant lacking the SH3 domain is phosphorylated upon CD3 cross-linking but not upon integrin beta-1 cross-linking. Tyrosine phosphorylation occurs upon stimulation of the G-protein coupled C1a calcitonin receptor. Calcitonin-stimulated tyrosine phosphorylation is mediated by calcium- and protein kinase C-dependent mechanisms and requires the integrity of the actin cytoskeleton. Phosphorylation at Ser-368 induces proteasomal degradation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35177
MaxQBiO35177
PaxDbiO35177
PeptideAtlasiO35177
PRIDEiO35177

PTM databases

iPTMnetiO35177
PhosphoSitePlusiO35177

Expressioni

Gene expression databases

BgeeiENSMUSG00000021365
ExpressionAtlasiO35177 baseline and differential
GenevisibleiO35177 MM

Interactioni

Subunit structurei

Homodimer. Can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. Forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase. Interacts with MICAL and TXNL4/DIM1 (By similarity). Interacts with BCAR3 and SH2D3C.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Sh2d3cQ9QZS82EBI-2642891,EBI-7964037

Protein-protein interaction databases

BioGridi201726, 5 interactors
DIPiDIP-57058N
IntActiO35177, 6 interactors
STRINGi10090.ENSMUSP00000021794

Structurei

3D structure databases

ProteinModelPortaliO35177
SMRiO35177
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 65SH3PROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni709 – 759Divergent helix-loop-helix motifAdd BLAST51

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi359 – 362Caspase cleavage related siteBy similarity4

Sequence similaritiesi

Belongs to the CAS family.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410IEDD Eukaryota
ENOG410ZSUM LUCA
GeneTreeiENSGT00490000043324
HOVERGENiHBG004354
InParanoidiO35177
KOiK16832
PhylomeDBiO35177
TreeFamiTF328782

Family and domain databases

CDDicd12002 SH3_NEDD9, 1 hit
Gene3Di1.20.120.830, 1 hit
InterProiView protein in InterPro
IPR021901 CAS_C
IPR037362 CAS_fam
IPR035746 NEDD9_SH3
IPR014928 Serine_rich_dom
IPR038319 Serine_rich_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR10654 PTHR10654, 1 hit
PfamiView protein in Pfam
PF12026 DUF3513, 1 hit
PF08824 Serine_rich, 1 hit
PF14604 SH3_9, 1 hit
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

O35177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWARNLMARA LYDNVPECAE ELAFRKGDIL TVIEQNTGGL EGWWLCSLHG
60 70 80 90 100
RQGIVPGNRV KLLIGPVQET PGHEQPTPGP MHQTFGQQKL YQVPNSQAAS
110 120 130 140 150
RDTIYQVPPS YQNQGIYQVP TGHGTPEQDV YQVPPSVQRN IGGTNGPLLS
160 170 180 190 200
KKVITPVRTG HGYVYEYPSR YQKDVYDVPP SHSTQGVYDI PPSSVKGPVF
210 220 230 240 250
SVPVGEIKPQ GVYDIPPTQG VYAIPPSACR DEAGLREKEY DFPPPMKQDG
260 270 280 290 300
KPDTRPEGVY DIPPTSTKTA GKDLHIKFPC DAPGGVEPMA RRHQSFSLHH
310 320 330 340 350
APSQLGQSGD TQSDAYDVPR GVQFLEVPTE TSEKANPEER DGVYDVPLHN
360 370 380 390 400
PADAKGSRDV VDGINRLSFS STGSTRSNMS TSSTSSKESS LSASPSQDKR
410 420 430 440 450
LRLDPDTAIE KLYRLQQTLE MGVCSLMSLV TTDWRCYGYM ERHINEIRTA
460 470 480 490 500
VDKVELFLRE YLHFAKGALA NASCLPELVL HNKMKRELQR VEDSHQILSQ
510 520 530 540 550
TSHDLNECSW SLNILAINKP QNKCDDLDRF VMVAKTVPDD AKQLTTTIST
560 570 580 590 600
YAETLFRADP ANSHLKNGPN SIMNSSEYTH PGSQMQPLHP GDYKAQVHSK
610 620 630 640 650
PLPPSLSKDQ PPDCGSSDGS ERSWMDDYDY VHLQGKEEFE RQQKELLEKE
660 670 680 690 700
NIMKQSKAQL EHHQLSQFQL LEQEITKPVE NDISKWKPSQ SLPTTNNSVG
710 720 730 740 750
AQDRQLLCFY YDQCETHFIS LLNAIDALFS CVSSAQPPRI FVAHSKFVIL
760 770 780 790 800
SAHKLVFIGD TLTRQVAAQD IRNKVRNSSN QLCEQLKTIV MATKMAALHY
810 820 830
PSTTALQEMV HQVTDLSRNA QLFKRSLLEM ATF
Length:833
Mass (Da):93,052
Last modified:June 20, 2003 - v2
Checksum:i78AA1B042775DC01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 4WAR → KYK in BAC35682 (PubMed:16141072).Curated3
Sequence conflicti2 – 4WAR → KYK in BAC32689 (PubMed:16141072).Curated3
Sequence conflicti2 – 4WAR → KYK in BAC28451 (PubMed:16141072).Curated3
Sequence conflicti55V → A in BAC32689 (PubMed:16141072).Curated1
Sequence conflicti126 – 127PE → QN in AAB71663 (Ref. 1) Curated2
Sequence conflicti137V → F in AAB71663 (Ref. 1) Curated1
Sequence conflicti149L → V in BAC32689 (PubMed:16141072).Curated1
Sequence conflicti161H → L in AAB71663 (Ref. 1) Curated1
Sequence conflicti313S → N in BAC27203 (PubMed:16141072).Curated1
Sequence conflicti485K → S in AAB71663 (Ref. 1) Curated1
Sequence conflicti504 – 506DLN → ELD in AAB71663 (Ref. 1) Curated3
Sequence conflicti526D → G in AAB71663 (Ref. 1) Curated1
Sequence conflicti604P → A in BAC35682 (PubMed:16141072).Curated1
Sequence conflicti612P → Q in BAC32689 (PubMed:16141072).Curated1
Sequence conflicti650E → A in AAB71663 (Ref. 1) Curated1
Sequence conflicti685K → R in BAC32689 (PubMed:16141072).Curated1
Sequence conflicti733 – 734SS → TP in AAB71663 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009366 mRNA Translation: AAB71663.1
AK030985 mRNA Translation: BAC27203.1
AK033729 mRNA Translation: BAC28451.1
AK046357 mRNA Translation: BAC32689.1
AK054179 mRNA Translation: BAC35682.1
AK083374 mRNA Translation: BAC38890.1
BC004696 mRNA Translation: AAH04696.1
BC053713 mRNA Translation: AAH53713.1
CCDSiCCDS49247.1
RefSeqiNP_001104794.1, NM_001111324.2
NP_059492.3, NM_017464.5
UniGeneiMm.288980

Genome annotation databases

EnsembliENSMUST00000021794; ENSMUSP00000021794; ENSMUSG00000021365
GeneIDi18003
KEGGimmu:18003
UCSCiuc007qfd.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCASL_MOUSE
AccessioniPrimary (citable) accession number: O35177
Secondary accession number(s): Q8BJL8
, Q8BK90, Q8BL52, Q8BM94, Q8BMI9, Q99KE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 20, 2003
Last modified: March 28, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health