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Protein

Potassium voltage-gated channel subfamily S member 1

Gene

Kcns1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1 and KCNB2; modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1 and KCNB2 (PubMed:9305895).1 Publication

GO - Molecular functioni

GO - Biological processi

  • potassium ion transmembrane transport Source: GO_Central
  • potassium ion transport Source: UniProtKB
  • protein homooligomerization Source: InterPro
  • regulation of delayed rectifier potassium channel activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_287159. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily S member 1
Alternative name(s):
Delayed-rectifier K(+) channel alpha subunit 1
Voltage-gated potassium channel subunit Kv9.1
Gene namesi
Name:Kcns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1197019. Kcns1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 186186CytoplasmicBy similarityAdd
BLAST
Transmembranei187 – 20822Helical; Name=Segment S1By similarityAdd
BLAST
Topological domaini209 – 23931ExtracellularBy similarityAdd
BLAST
Transmembranei240 – 26223Helical; Name=Segment S2By similarityAdd
BLAST
Topological domaini263 – 27311CytoplasmicBy similarityAdd
BLAST
Transmembranei274 – 29118Helical; Name=Segment S3By similarityAdd
BLAST
Topological domaini292 – 30918ExtracellularBy similarityAdd
BLAST
Transmembranei310 – 33021Helical; Voltage-sensor; Name=Segment S4By similarityAdd
BLAST
Topological domaini331 – 34515CytoplasmicBy similarityAdd
BLAST
Transmembranei346 – 36722Helical; Name=Segment S5By similarityAdd
BLAST
Topological domaini368 – 37912ExtracellularBy similarityAdd
BLAST
Intramembranei380 – 39112Helical; Name=Pore helixBy similarityAdd
BLAST
Intramembranei392 – 3998By similarity
Topological domaini400 – 4067ExtracellularBy similarity
Transmembranei407 – 43529Helical; Name=Segment S6By similarityAdd
BLAST
Topological domaini436 – 49762CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Potassium voltage-gated channel subfamily S member 1PRO_0000054082Add
BLAST

Proteomic databases

PRIDEiO35173.

PTM databases

PhosphoSiteiO35173.

Expressioni

Tissue specificityi

Detected in brain, but not in the other tissues tested. The highest levels of expression are in olfactory bulb, cerebral cortex, hippocampus, habenula, basolateral amygdaloid nuclei and cerebellum (PubMed:9305895).1 Publication

Gene expression databases

BgeeiO35173.
CleanExiMM_KCNS1.
ExpressionAtlasiO35173. baseline and differential.
GenevisibleiO35173. MM.

Interactioni

Subunit structurei

Heterotetramer with KCNB1 and KCNB2 (PubMed:9305895). Does not form homomultimers (PubMed:9305895).1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038901.

Structurei

3D structure databases

ProteinModelPortaliO35173.
SMRiO35173. Positions 19-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi392 – 3976Selectivity filterBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi219 – 22810Poly-Ala

Domaini

The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118981.
HOGENOMiHOG000231016.
HOVERGENiHBG052230.
InParanoidiO35173.
KOiK04931.
OMAiWLTLENP.
OrthoDBiEOG7CRTPP.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

O35173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSEFPGPGS RVPWRPRDEA LRVNVGGVRR LLSARALARF PGTRLGRLQA
60 70 80 90 100
AASEEQARRL CDDYDAAAHE FYFDRHPGFF LGLLHFYRTG HLHVLDELCV
110 120 130 140 150
FAFGQEADYW GLGENALATC CRARYLERRV ARPRAWDEDS DAPSSVDPCP
160 170 180 190 200
DEISDVQREL ARYGAARCGR LRRRLWLTME NPGYSLPSKL FSCVSIGVVL
210 220 230 240 250
ASIAAMCIHS LPEYQAREAA AAVAAVAAGR SAEEVRDDPV LRRLEYFCIA
260 270 280 290 300
WFSFEVSSRL LLAPSTRNFF CHPLNLIDIV SVLPFYLTLL AGAALGDQRG
310 320 330 340 350
ASGEELGDLG KVVQVFRLMR IFRVLKLARH STGLRSLGAT LKHSYREVGI
360 370 380 390 400
LLLYLAVGVS VFSGVAYTAE EENEGFHTIP ACWWWGTVSM TTVGYGDVVP
410 420 430 440 450
ETVGGKLAAS GCILGGILVV ALPITIIFNK FSHFYRRQKA LEAAVRSSGQ
460 470 480 490
REFEDLLSSV DGVSDVSLET SRDTSQEGRS TDLETQAPRE PAKSHSY
Length:497
Mass (Da):54,918
Last modified:July 27, 2011 - v2
Checksum:iF734A3D4B093BEBE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti440 – 4401A → G in AAB72050 (PubMed:9305895).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008573 mRNA. Translation: AAB72050.1.
AL591512 Genomic DNA. Translation: CAM21684.1.
CH466551 Genomic DNA. Translation: EDL06364.1.
CCDSiCCDS17022.1.
RefSeqiNP_032461.2. NM_008435.2.
UniGeneiMm.6217.

Genome annotation databases

EnsembliENSMUST00000045196; ENSMUSP00000038901; ENSMUSG00000040164.
GeneIDi16538.
KEGGimmu:16538.
UCSCiuc008ntv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008573 mRNA. Translation: AAB72050.1.
AL591512 Genomic DNA. Translation: CAM21684.1.
CH466551 Genomic DNA. Translation: EDL06364.1.
CCDSiCCDS17022.1.
RefSeqiNP_032461.2. NM_008435.2.
UniGeneiMm.6217.

3D structure databases

ProteinModelPortaliO35173.
SMRiO35173. Positions 19-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038901.

PTM databases

PhosphoSiteiO35173.

Proteomic databases

PRIDEiO35173.

Protocols and materials databases

DNASUi16538.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045196; ENSMUSP00000038901; ENSMUSG00000040164.
GeneIDi16538.
KEGGimmu:16538.
UCSCiuc008ntv.1. mouse.

Organism-specific databases

CTDi3787.
MGIiMGI:1197019. Kcns1.

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118981.
HOGENOMiHOG000231016.
HOVERGENiHBG052230.
InParanoidiO35173.
KOiK04931.
OMAiWLTLENP.
OrthoDBiEOG7CRTPP.
TreeFamiTF313103.

Enzyme and pathway databases

ReactomeiREACT_287159. Voltage gated Potassium channels.

Miscellaneous databases

NextBioi289991.
PROiO35173.
SOURCEiSearch...

Gene expression databases

BgeeiO35173.
CleanExiMM_KCNS1.
ExpressionAtlasiO35173. baseline and differential.
GenevisibleiO35173. MM.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "New modulatory alpha subunits for mammalian Shab K+ channels."
    Salinas M., Duprat F., Heurteaux C., Hugnot J.-P., Lazdunski M.
    J. Biol. Chem. 272:24371-24379(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKCNS1_MOUSE
AccessioniPrimary (citable) accession number: O35173
Secondary accession number(s): A2A5M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.