ID   GOSR2_MOUSE             Reviewed;         212 AA.
AC   O35166; Q3UDN0; Q9CR77;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   31-AUG-2004, sequence version 2.
DT   24-JAN-2024, entry version 158.
DE   RecName: Full=Golgi SNAP receptor complex member 2;
DE   AltName: Full=27 kDa Golgi SNARE protein;
DE   AltName: Full=Membrin;
GN   Name=Gosr2; Synonyms=Gs27;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9349823; DOI=10.1038/39923;
RA   Lowe S.L., Peter F., Subramaniam V.N., Wong S.H., Hong W.;
RT   "A SNARE involved in protein transport through the Golgi apparatus.";
RL   Nature 389:881-884(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Bone marrow, Embryo, and Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N, and FVB/N-3; TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Heart, Kidney, Lung, Pancreas, Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Involved in transport of proteins from the cis/medial-Golgi
CC       to the trans-Golgi network. {ECO:0000250|UniProtKB:O35165}.
CC   -!- SUBUNIT: Part of a unique SNARE complex composed of the Golgi SNAREs
CC       GOSR1, STX5 and YKT6. {ECO:0000250|UniProtKB:O35165}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC       {ECO:0000250|UniProtKB:O35165}; Single-pass type IV membrane protein
CC       {ECO:0000255}. Golgi apparatus membrane {ECO:0000250|UniProtKB:O35165}.
CC       Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:O35165}.
CC       Note=Concentrated most in the intermediate compartment/cis-Golgi
CC       network and the cis-Golgi cisternae 1 and 2. Greatly reduced in
CC       concentration at the trans end of the Golgi apparatus.
CC       {ECO:0000250|UniProtKB:O35165}.
CC   -!- SIMILARITY: Belongs to the GOSR2 family. {ECO:0000305}.
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DR   EMBL; AF007550; AAB82653.1; -; mRNA.
DR   EMBL; AK005697; BAB24194.1; -; mRNA.
DR   EMBL; AK013052; BAB28622.1; -; mRNA.
DR   EMBL; AK150004; BAE29231.1; -; mRNA.
DR   EMBL; BC008525; AAH08525.1; -; mRNA.
DR   EMBL; BC051253; AAH51253.1; -; mRNA.
DR   CCDS; CCDS25521.1; -.
DR   RefSeq; NP_062624.2; NM_019650.3.
DR   AlphaFoldDB; O35166; -.
DR   SMR; O35166; -.
DR   BioGRID; 208018; 3.
DR   STRING; 10090.ENSMUSP00000021329; -.
DR   PhosphoSitePlus; O35166; -.
DR   EPD; O35166; -.
DR   MaxQB; O35166; -.
DR   PaxDb; 10090-ENSMUSP00000021329; -.
DR   ProteomicsDB; 271132; -.
DR   Pumba; O35166; -.
DR   DNASU; 56494; -.
DR   Ensembl; ENSMUST00000021329.14; ENSMUSP00000021329.8; ENSMUSG00000020946.14.
DR   GeneID; 56494; -.
DR   KEGG; mmu:56494; -.
DR   UCSC; uc007lvo.2; mouse.
DR   AGR; MGI:1927204; -.
DR   CTD; 9570; -.
DR   MGI; MGI:1927204; Gosr2.
DR   VEuPathDB; HostDB:ENSMUSG00000020946; -.
DR   eggNOG; KOG3251; Eukaryota.
DR   GeneTree; ENSGT00950000183192; -.
DR   InParanoid; O35166; -.
DR   OMA; RMRVDQL; -.
DR   OrthoDB; 5477092at2759; -.
DR   PhylomeDB; O35166; -.
DR   TreeFam; TF313702; -.
DR   Reactome; R-MMU-204005; COPII-mediated vesicle transport.
DR   Reactome; R-MMU-5694530; Cargo concentration in the ER.
DR   Reactome; R-MMU-6807878; COPI-mediated anterograde transport.
DR   Reactome; R-MMU-6811438; Intra-Golgi traffic.
DR   BioGRID-ORCS; 56494; 20 hits in 79 CRISPR screens.
DR   ChiTaRS; Gosr2; mouse.
DR   PRO; PR:O35166; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   RNAct; O35166; Protein.
DR   Bgee; ENSMUSG00000020946; Expressed in metanephric ureteric bud and 262 other cell types or tissues.
DR   ExpressionAtlas; O35166; baseline and differential.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:MGI.
DR   GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR   GO; GO:0000139; C:Golgi membrane; IDA:MGI.
DR   GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0031201; C:SNARE complex; ISS:UniProtKB.
DR   GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR   GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; IDA:MGI.
DR   CDD; cd15863; SNARE_GS27; 1.
DR   InterPro; IPR027027; GOSR2/Membrin/Bos1.
DR   PANTHER; PTHR21230:SF1; GOLGI SNAP RECEPTOR COMPLEX MEMBER 2; 1.
DR   PANTHER; PTHR21230; VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED; 1.
DR   Pfam; PF12352; V-SNARE_C; 1.
DR   PIRSF; PIRSF028865; Membrin-2; 1.
DR   SUPFAM; SSF58038; SNARE fusion complex; 1.
DR   Genevisible; O35166; MM.
PE   1: Evidence at protein level;
KW   Acetylation; Coiled coil; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW   Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..212
FT                   /note="Golgi SNAP receptor complex member 2"
FT                   /id="PRO_0000212550"
FT   TOPO_DOM        1..190
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..211
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        212
FT                   /note="Vesicular"
FT                   /evidence="ECO:0000255"
FT   COILED          60..92
FT                   /evidence="ECO:0000255"
FT   MOTIF           118..120
FT                   /note="IxM motif; signal for cargo packaging into COPII-
FT                   coated vesicles"
FT                   /evidence="ECO:0000250|UniProtKB:O14653"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:O14653"
FT   CONFLICT        99
FT                   /note="R -> I (in Ref. 1; AAB82653)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   212 AA;  24725 MW;  1622371B06090A6C CRC64;
     MEPLYQQTNK QVQEIQSHMG RLERADKQSV HLVENEIQAS IEQIFSHLER LEILSSKEPL
     NRRQNAKLRV DQLKYDVQHL QTALRNFQHR RQVREQQERQ RDELLSRTFT TNDSDTTIPM
     DESLQFNSSL HNIHHGMDDL IGGGHSILEG LRAQRLTLKG TQKKILDIAN MLGLSNTVMR
     LIEKRAFQDK YFMIGGMLLT CAVMFLVVQY LT
//