O35158 (CDON_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell adhesion molecule-related/down-regulated by oncogenes

Gene names

Name:	Cdon
Synonyms:	Cdo

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells By similarity.
Subunit structure	Part of a complex that contains BOC, CDON, NEO1, cadherins and CTNNB1. Interacts with NTN3 By similarity. Interacts with PTCH1. Interacts with GAS1. Ref.2
Subcellular location	Membrane; Single-pass membrane protein Potential Ref.1.
Tissue specificity	Detected at low levels in stomach, spleen, brain, large intestine and lung. Ref.1
Induction	Down-regulated in actively dividing cells. Not detectable in suspension cultures. Accumulates only in cells that are attached to a solid substrate (in vitro). Ref.1
Post-translational modification	N-glycosylated. Ref.1
Sequence similarities	Contains 3 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from mutant phenotype Ref.1. Source: RGD smoothened signaling pathway Inferred from direct assay PubMed 16647303. Source: MGI
Cellular_component	cell surface Inferred from direct assay Ref.1. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O35158-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O35158-2)

The sequence of this isoform differs from the canonical sequence as follows:
     400-516: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 1256	1232	Cell adhesion molecule-related/down-regulated by oncogenes		PRO_0000234056
Regions
	Topological domain	25 – 963	939	Extracellular Potential
	Transmembrane	964 – 984	21	Helical; Potential
	Topological domain	985 – 1256	272	Cytoplasmic Potential
	Domain	28 – 113	86	Ig-like C2-type 1
	Domain	119 – 203	85	Ig-like C2-type 2
	Domain	224 – 305	82	Ig-like C2-type 3
	Domain	310 – 396	87	Ig-like C2-type 4
	Domain	405 – 516	112	Ig-like C2-type 5
	Domain	574 – 670	97	Fibronectin type-III 1
	Domain	718 – 810	93	Fibronectin type-III 2
	Domain	823 – 920	98	Fibronectin type-III 3
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	286	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	294	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential
	Glycosylation	570	1	N-linked (GlcNAc...) Potential
	Glycosylation	870	1	N-linked (GlcNAc...) Potential
	Disulfide bond	49 ↔ 96		By similarity
	Disulfide bond	140 ↔ 190		By similarity
	Disulfide bond	242 ↔ 289		By similarity
	Disulfide bond	333 ↔ 380		By similarity
	Disulfide bond	426 ↔ 500		By similarity
Natural variations
	Alternative sequence	400 – 516	117	Missing in isoform 2.		VSP_018202
Experimental info
	Mutagenesis	686	1	P → A: Fails to support induction of the Sonic hedgehog/SSH receptors PTCH1 and GLI1. The mutant protein retains very little residual activity even during overexpression and interacts with SHH, but does not interact with PTCH1, BOC, or GAS1. Ref.2
	Mutagenesis	777	1	V → E: Fails to support induction of the Sonic hedgehog/SSH receptors PTCH1 and GLI1. The mutant protein retains some residual activity during overexpression. The mutant protein interacts with SHH, but does not interatc with PTCH1. The mutation protein has a shorted half-life compared to wild-type and may have an altered conformation resulting in destabilization. Ref.2
	Mutagenesis	787	1	I → A: Fails to support induction of the Sonic hedgehog/SSH receptors PTCH1 and GLI1. The mutant protein retains some residual activity during overexpression. The mutant protein interacts with SHH, but does not interact with GAS1. Ref.2
	Mutagenesis	937	1	S → R: Fails to support induction of the Sonic hedgehog/SSH receptors PTCH1 and GLI1. The mutant protein retains some residual activity during overexpression. The mutant protein interacts with SHH, but does not interact with PTCH1 or GAS1. The mutation protein has a shorted half-life compared to wild-type and may have an altered conformation resulting in destabilization. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 2. Checksum: 775805754F0C2EA4	FASTA	1,256	136,204
	10 20 30 40 50 60 MHPDLGPLWK LLYVLVILCS SVSSDLATYF ISEPLSAVQK LGRPVVLHCS AKPVTARISW 70 80 90 100 110 120 LHNGKRLDRN TEQIKIHRGT LTILSLNPSL SGCYQCVANN SVGAVVSGPA TVSADALADF 130 140 150 160 170 180 DSSTMHVITA EKKNTGFIGC RVPESNPKAE VRYKIRGKWL MYSTGNYIIL PSGNLQILNV 190 200 210 220 230 240 SSKDKGSYKC AAYNPVTSEL KVEPAGRKLL VSRPSSDGFH ILHPALSQAL AVLPHSPVTL 250 260 270 280 290 300 ECVVSGVPAS QVYWLKDGQD CLSGSNWRRL YSHLATASID PADSGNYSCV VGNNSSGDVK 310 320 330 340 350 360 HVTYTVNVLE HASISKGLHD QKVSLGATVR FTCEVHGNPA PNRTWFHNAQ PIRPSSRHLT 370 380 390 400 410 420 EGSVLKITGV IMEDSGLYQC MADNGIGFMQ STGRLQIEQD SGQRPVIVTA PANVEVTDGD 430 440 450 460 470 480 FVTLSCNATG EPVPVIHWYG RHGLITSHPS QVLRSKSRKS HLFRPGDLDP EPVYLIMSQA 490 500 510 520 530 540 GSSSLSIQAV TREHAGKYTC EAVNKHGSTQ SEAFLTVVPF ETNTKAEPVT PSEASQNDER 550 560 570 580 590 600 DPRDGSESGL LNLFPVKVHS GGVELPAEKN ASVPDAPNIL SPPQTHMPDT YTLVWRTGRD 610 620 630 640 650 660 GGMPINAYFV KYRKLDDGSG AVGSWHTVRV PGSESELHLT ELEPSSLYEV LMVARSAVGE 670 680 690 700 710 720 GQPAMLTFRT SKEKMASSKN TQASFPPVGI PKRPVTSEAS NSNFGVVLTD SSRHSGVPEA 730 740 750 760 770 780 PDRPTISMAS ETSVYVTWIP RANGGSPITA FKVEYKRMKS SDWLVAAEDI PPSKLSVEVR 790 800 810 820 830 840 SLEPGSIYKF RVIVINHYGE SFRSSASRPY QVAGFPNRFS NRPITGPHIA YTEAVSDTQI 850 860 870 880 890 900 MLKWTYIPSS NNNTPIQGFY IYYRPTDSDN DSDYKRDVVE GSKQWHTIGH LQPETSYDIK 910 920 930 940 950 960 MQCFNEGGES EFSNVMICET KVKRVPGASE YPMKELSTPP SSSGNGGNVG PATSPARSSD 970 980 990 1000 1010 1020 MLYLIVGCVL GVMVLILLVF IALCLWKSRQ QSAIQKYDPP GYLYQGSEIN GQMVEYTTLS 1030 1040 1050 1060 1070 1080 GTARINGSVH GGFLSKGSLS NGCSHLHHKG PNGVNGILNG TINGGLYSAH TSSLTRTCVE 1090 1100 1110 1120 1130 1140 FEHPHHLVNG GAVYTAVPQM DPLECINCRN CRNNNRCFTK TNSPLPVVPV VASYPQDGLE 1150 1160 1170 1180 1190 1200 MKPLGVMKFP VCPVSTVPDG GQIPEECLKD SVAPAPTQRT CRQDNTSDIN SDSTEDTAEF 1210 1220 1230 1240 1250 NRGDSSGHSE AEDKVFSWSP LILSPVLEDC SEKTAWSPPG PPLDGLSVVL QQAQET
	Isoform 2 [UniParc]. Checksum: CDAC81E99545870D	FASTA	1,139	123,594
	10 20 30 40 50 60 MHPDLGPLWK LLYVLVILCS SVSSDLATYF ISEPLSAVQK LGRPVVLHCS AKPVTARISW 70 80 90 100 110 120 LHNGKRLDRN TEQIKIHRGT LTILSLNPSL SGCYQCVANN SVGAVVSGPA TVSADALADF 130 140 150 160 170 180 DSSTMHVITA EKKNTGFIGC RVPESNPKAE VRYKIRGKWL MYSTGNYIIL PSGNLQILNV 190 200 210 220 230 240 SSKDKGSYKC AAYNPVTSEL KVEPAGRKLL VSRPSSDGFH ILHPALSQAL AVLPHSPVTL 250 260 270 280 290 300 ECVVSGVPAS QVYWLKDGQD CLSGSNWRRL YSHLATASID PADSGNYSCV VGNNSSGDVK 310 320 330 340 350 360 HVTYTVNVLE HASISKGLHD QKVSLGATVR FTCEVHGNPA PNRTWFHNAQ PIRPSSRHLT 370 380 390 400 410 420 EGSVLKITGV IMEDSGLYQC MADNGIGFMQ STGRLQIEQV VPFETNTKAE PVTPSEASQN 430 440 450 460 470 480 DERDPRDGSE SGLLNLFPVK VHSGGVELPA EKNASVPDAP NILSPPQTHM PDTYTLVWRT 490 500 510 520 530 540 GRDGGMPINA YFVKYRKLDD GSGAVGSWHT VRVPGSESEL HLTELEPSSL YEVLMVARSA 550 560 570 580 590 600 VGEGQPAMLT FRTSKEKMAS SKNTQASFPP VGIPKRPVTS EASNSNFGVV LTDSSRHSGV 610 620 630 640 650 660 PEAPDRPTIS MASETSVYVT WIPRANGGSP ITAFKVEYKR MKSSDWLVAA EDIPPSKLSV 670 680 690 700 710 720 EVRSLEPGSI YKFRVIVINH YGESFRSSAS RPYQVAGFPN RFSNRPITGP HIAYTEAVSD 730 740 750 760 770 780 TQIMLKWTYI PSSNNNTPIQ GFYIYYRPTD SDNDSDYKRD VVEGSKQWHT IGHLQPETSY 790 800 810 820 830 840 DIKMQCFNEG GESEFSNVMI CETKVKRVPG ASEYPMKELS TPPSSSGNGG NVGPATSPAR 850 860 870 880 890 900 SSDMLYLIVG CVLGVMVLIL LVFIALCLWK SRQQSAIQKY DPPGYLYQGS EINGQMVEYT 910 920 930 940 950 960 TLSGTARING SVHGGFLSKG SLSNGCSHLH HKGPNGVNGI LNGTINGGLY SAHTSSLTRT 970 980 990 1000 1010 1020 CVEFEHPHHL VNGGAVYTAV PQMDPLECIN CRNCRNNNRC FTKTNSPLPV VPVVASYPQD 1030 1040 1050 1060 1070 1080 GLEMKPLGVM KFPVCPVSTV PDGGQIPEEC LKDSVAPAPT QRTCRQDNTS DINSDSTEDT 1090 1100 1110 1120 1130 AEFNRGDSSG HSEAEDKVFS WSPLILSPVL EDCSEKTAWS PPGPPLDGLS VVLQQAQET

References

[1]	"CDO: an oncogene-, serum-, and anchorage-regulated member of the Ig/fibronectin type III repeat family." Kang J.-S., Gao M., Feinleib J.L., Cotter P.D., Guadagno S.N., Krauss R.S. J. Cell Biol. 138:203-213(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INDUCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, TISSUE SPECIFICITY. Tissue: Embryonic fibroblast.
[2]	"Mutations in CDON, encoding a hedgehog receptor, result in holoprosencephaly and defective interactions with other hedgehog receptors." Bae G.U., Domene S., Roessler E., Schachter K., Kang J.S., Muenke M., Krauss R.S. Am. J. Hum. Genet. 89:231-240(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PTCH1, BOC AND GAS1, MUTAGENESIS OF PRO-686; VAL-777; ILE-787 AND SER-937.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004840 mRNA. Translation: AAC34735.1.
IPI	IPI00206284. IPI00758456.
PIR	T03096.
RefSeq	NP_059054.1. NM_017358.1.
UniGene	Rn.10818.
3D structure databases
ProteinModelPortal	O35158.
SMR	O35158. Positions 718-814, 820-928.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-5117456.
STRING	10116.ENSRNOP00000016247.
Proteomic databases
PaxDb	O35158.
PRIDE	O35158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	50938.
KEGG	rno:50938.
UCSC	RGD:708433. rat.
Organism-specific databases
CTD	50937.
RGD	708433. Cdon.
Phylogenomic databases
eggNOG	NOG150729.
HOGENOM	HOG000060072.
HOVERGEN	HBG081073.
InParanoid	O35158.
OrthoDB	EOG4GTKC2.
Gene expression databases
ArrayExpress	O35158.
Genevestigator	O35158.
GermOnline	ENSRNOG00000011789. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.40.10. 8 hits.
InterPro	IPR003961. Fibronectin_type3. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. [Graphical view]
Pfam	PF00041. fn3. 3 hits. PF07679. I-set. 3 hits. [Graphical view]
SMART	SM00060. FN3. 3 hits. SM00409. IG. 1 hit. SM00408. IGc2. 4 hits. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 3 hits.
PROSITE	PS50853. FN3. 3 hits. PS50835. IG_LIKE. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	610598.

Entry information

Entry name

CDON_RAT

Accession

Primary (citable) accession number: O35158

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program