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O35158 (CDON_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell adhesion molecule-related/down-regulated by oncogenes
Gene names
Name:Cdon
Synonyms:Cdo
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1256 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells By similarity.

Subunit structure

Part of a complex that contains BOC, CDON, NEO1, cadherins and CTNNB1. Interacts with NTN3. Interacts with DHH, IHH and SHH By similarity. Interacts with PTCH1. Interacts with GAS1. Ref.2

Subcellular location

Cell membrane; Single-pass membrane protein Ref.1 Ref.2.

Tissue specificity

Detected at low levels in stomach, spleen, brain, large intestine and lung. Ref.1

Induction

Down-regulated in actively dividing cells. Not detectable in suspension cultures. Accumulates only in cells that are attached to a solid substrate (in vitro). Ref.1

Post-translational modification

N-glycosylated. Ref.1

Sequence similarities

Contains 3 fibronectin type-III domains.

Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ABL1P005194EBI-7016767,EBI-375543From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35158-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35158-2)

The sequence of this isoform differs from the canonical sequence as follows:
     400-516: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 12561232Cell adhesion molecule-related/down-regulated by oncogenes
PRO_0000234056

Regions

Topological domain25 – 963939Extracellular Potential
Transmembrane964 – 98421Helical; Potential
Topological domain985 – 1256272Cytoplasmic Potential
Domain28 – 11386Ig-like C2-type 1
Domain119 – 20385Ig-like C2-type 2
Domain224 – 30582Ig-like C2-type 3
Domain310 – 39687Ig-like C2-type 4
Domain405 – 516112Ig-like C2-type 5
Domain573 – 674102Fibronectin type-III 1
Domain720 – 81596Fibronectin type-III 2
Domain823 – 923101Fibronectin type-III 3

Amino acid modifications

Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1791N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential
Glycosylation3421N-linked (GlcNAc...) Potential
Glycosylation4271N-linked (GlcNAc...) Potential
Glycosylation5701N-linked (GlcNAc...) Potential
Glycosylation8701N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 96 By similarity
Disulfide bond140 ↔ 190 By similarity
Disulfide bond242 ↔ 289 By similarity
Disulfide bond333 ↔ 380 By similarity
Disulfide bond426 ↔ 500 By similarity

Natural variations

Alternative sequence400 – 516117Missing in isoform 2.
VSP_018202

Experimental info

Mutagenesis6861P → A: Fails to support induction of the Sonic hedgehog/SSH receptors PTCH1 and GLI1. The mutant protein retains very little residual activity even during overexpression and interacts with SHH, but does not interact with PTCH1, BOC, or GAS1. Ref.2
Mutagenesis7771V → E: Fails to support induction of the Sonic hedgehog/SSH receptors PTCH1 and GLI1. The mutant protein retains some residual activity during overexpression. The mutant protein interacts with SHH, but does not interatc with PTCH1. The mutation protein has a shorted half-life compared to wild-type and may have an altered conformation resulting in destabilization. Ref.2
Mutagenesis7871I → A: Fails to support induction of the Sonic hedgehog/SSH receptors PTCH1 and GLI1. The mutant protein retains some residual activity during overexpression. The mutant protein interacts with SHH, but does not interact with GAS1. Ref.2
Mutagenesis9371S → R: Fails to support induction of the Sonic hedgehog/SSH receptors PTCH1 and GLI1. The mutant protein retains some residual activity during overexpression. The mutant protein interacts with SHH, but does not interact with PTCH1 or GAS1. The mutation protein has a shorted half-life compared to wild-type and may have an altered conformation resulting in destabilization. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 2.
Checksum: 775805754F0C2EA4

FASTA1,256136,204
        10         20         30         40         50         60 
MHPDLGPLWK LLYVLVILCS SVSSDLATYF ISEPLSAVQK LGRPVVLHCS AKPVTARISW 

        70         80         90        100        110        120 
LHNGKRLDRN TEQIKIHRGT LTILSLNPSL SGCYQCVANN SVGAVVSGPA TVSADALADF 

       130        140        150        160        170        180 
DSSTMHVITA EKKNTGFIGC RVPESNPKAE VRYKIRGKWL MYSTGNYIIL PSGNLQILNV 

       190        200        210        220        230        240 
SSKDKGSYKC AAYNPVTSEL KVEPAGRKLL VSRPSSDGFH ILHPALSQAL AVLPHSPVTL 

       250        260        270        280        290        300 
ECVVSGVPAS QVYWLKDGQD CLSGSNWRRL YSHLATASID PADSGNYSCV VGNNSSGDVK 

       310        320        330        340        350        360 
HVTYTVNVLE HASISKGLHD QKVSLGATVR FTCEVHGNPA PNRTWFHNAQ PIRPSSRHLT 

       370        380        390        400        410        420 
EGSVLKITGV IMEDSGLYQC MADNGIGFMQ STGRLQIEQD SGQRPVIVTA PANVEVTDGD 

       430        440        450        460        470        480 
FVTLSCNATG EPVPVIHWYG RHGLITSHPS QVLRSKSRKS HLFRPGDLDP EPVYLIMSQA 

       490        500        510        520        530        540 
GSSSLSIQAV TREHAGKYTC EAVNKHGSTQ SEAFLTVVPF ETNTKAEPVT PSEASQNDER 

       550        560        570        580        590        600 
DPRDGSESGL LNLFPVKVHS GGVELPAEKN ASVPDAPNIL SPPQTHMPDT YTLVWRTGRD 

       610        620        630        640        650        660 
GGMPINAYFV KYRKLDDGSG AVGSWHTVRV PGSESELHLT ELEPSSLYEV LMVARSAVGE 

       670        680        690        700        710        720 
GQPAMLTFRT SKEKMASSKN TQASFPPVGI PKRPVTSEAS NSNFGVVLTD SSRHSGVPEA 

       730        740        750        760        770        780 
PDRPTISMAS ETSVYVTWIP RANGGSPITA FKVEYKRMKS SDWLVAAEDI PPSKLSVEVR 

       790        800        810        820        830        840 
SLEPGSIYKF RVIVINHYGE SFRSSASRPY QVAGFPNRFS NRPITGPHIA YTEAVSDTQI 

       850        860        870        880        890        900 
MLKWTYIPSS NNNTPIQGFY IYYRPTDSDN DSDYKRDVVE GSKQWHTIGH LQPETSYDIK 

       910        920        930        940        950        960 
MQCFNEGGES EFSNVMICET KVKRVPGASE YPMKELSTPP SSSGNGGNVG PATSPARSSD 

       970        980        990       1000       1010       1020 
MLYLIVGCVL GVMVLILLVF IALCLWKSRQ QSAIQKYDPP GYLYQGSEIN GQMVEYTTLS 

      1030       1040       1050       1060       1070       1080 
GTARINGSVH GGFLSKGSLS NGCSHLHHKG PNGVNGILNG TINGGLYSAH TSSLTRTCVE 

      1090       1100       1110       1120       1130       1140 
FEHPHHLVNG GAVYTAVPQM DPLECINCRN CRNNNRCFTK TNSPLPVVPV VASYPQDGLE 

      1150       1160       1170       1180       1190       1200 
MKPLGVMKFP VCPVSTVPDG GQIPEECLKD SVAPAPTQRT CRQDNTSDIN SDSTEDTAEF 

      1210       1220       1230       1240       1250 
NRGDSSGHSE AEDKVFSWSP LILSPVLEDC SEKTAWSPPG PPLDGLSVVL QQAQET 

« Hide

Isoform 2 [UniParc].

Checksum: CDAC81E99545870D
Show »

FASTA1,139123,594

References

[1]"CDO: an oncogene-, serum-, and anchorage-regulated member of the Ig/fibronectin type III repeat family."
Kang J.-S., Gao M., Feinleib J.L., Cotter P.D., Guadagno S.N., Krauss R.S.
J. Cell Biol. 138:203-213(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INDUCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, TISSUE SPECIFICITY.
Tissue: Embryonic fibroblast.
[2]"Mutations in CDON, encoding a hedgehog receptor, result in holoprosencephaly and defective interactions with other hedgehog receptors."
Bae G.U., Domene S., Roessler E., Schachter K., Kang J.S., Muenke M., Krauss R.S.
Am. J. Hum. Genet. 89:231-240(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PTCH1, BOC AND GAS1, SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-686; VAL-777; ILE-787 AND SER-937.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF004840 mRNA. Translation: AAC34735.1.
PIRT03096.
RefSeqNP_059054.1. NM_017358.1. [O35158-1]
UniGeneRn.10818.

3D structure databases

ProteinModelPortalO35158.
SMRO35158. Positions 718-814, 820-928.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35158. 1 interaction.
MINTMINT-5117456.
STRING10116.ENSRNOP00000016247.

Proteomic databases

PaxDbO35158.
PRIDEO35158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID50938.
KEGGrno:50938.
UCSCRGD:708433. rat. [O35158-1]

Organism-specific databases

CTD50937.
RGD708433. Cdon.

Phylogenomic databases

eggNOGNOG150729.
HOGENOMHOG000060072.
HOVERGENHBG081073.
InParanoidO35158.
PhylomeDBO35158.

Gene expression databases

GenevestigatorO35158.

Family and domain databases

Gene3D2.60.40.10. 8 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamPF00041. fn3. 3 hits.
PF07679. I-set. 3 hits.
[Graphical view]
SMARTSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio610598.
PROO35158.

Entry information

Entry nameCDON_RAT
AccessionPrimary (citable) accession number: O35158
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families