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Protein

Zinc transporter 4

Gene

Slc30a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Probably involved in zinc transport out of the cytoplasm, may be by sequestration into an intracellular compartment.

GO - Molecular functioni

GO - Biological processi

  • regulation of sequestering of zinc ion Source: MGI
  • response to toxic substance Source: MGI
  • zinc II ion transport Source: UniProtKB-KW
  • zinc ion homeostasis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport, Zinc transport

Keywords - Ligandi

Zinc

Protein family/group databases

TCDBi2.A.4.3.3. the cation diffusion facilitator (cdf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc transporter 4
Short name:
ZnT-4
Alternative name(s):
Lethal milk protein
Solute carrier family 30 member 4
Gene namesi
Name:Slc30a4
Synonyms:Lm, Znt4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1345282. Slc30a4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 113113CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei114 – 13421HelicalSequence AnalysisAdd
BLAST
Topological domaini135 – 1439VacuolarSequence Analysis
Transmembranei144 – 16421HelicalSequence AnalysisAdd
BLAST
Topological domaini165 – 17814CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei179 – 19921HelicalSequence AnalysisAdd
BLAST
Topological domaini200 – 21617VacuolarSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Topological domaini238 – 27538CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei276 – 29621HelicalSequence AnalysisAdd
BLAST
Topological domaini297 – 31115VacuolarSequence AnalysisAdd
BLAST
Transmembranei312 – 33221HelicalSequence AnalysisAdd
BLAST
Topological domaini333 – 43098CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Slc30a4 are the cause of the lethal milk (lm) phenotype. Mice with lm are defective in zinc transport into breast milk, due to a premature translation termination codon at position 297. Only homozygous mutant adults develop dermatitis, skin lesions, and hair loss due to a systemic zinc deficiency. However, neonatal mice (of any genotype) suckled on homozygous mutant female also develop symptoms characteristic of nutritional zinc deficiency, including dermatitis, alopecia and stunted growth.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430Zinc transporter 4PRO_0000206100Add
BLAST

Proteomic databases

PRIDEiO35149.

PTM databases

PhosphoSiteiO35149.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in the brain and in mammary epithelial cell lines.

Gene expression databases

BgeeiO35149.
ExpressionAtlasiO35149. baseline and differential.
GenevisibleiO35149. MM.

Interactioni

Protein-protein interaction databases

IntActiO35149. 1 interaction.
MINTiMINT-1605304.
STRINGi10090.ENSMUSP00000005952.

Structurei

3D structure databases

ProteinModelPortaliO35149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 9680Asp-rich (acidic)Add
BLAST
Compositional biasi241 – 2499His-rich

Domaini

Contains a histidine-rich region which is a ligand for zinc and an aspartate-rich region which is a potential ligand for zinc.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1230.
GeneTreeiENSGT00550000074381.
HOGENOMiHOG000079023.
HOVERGENiHBG003345.
InParanoidiO35149.
KOiK14691.
OMAiGLNMDPC.
OrthoDBiEOG7S4X68.
TreeFamiTF313382.

Family and domain databases

Gene3Di1.20.1510.10. 1 hit.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 1 hit.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.

Sequencei

Sequence statusi: Complete.

O35149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPGAWKRL KSLLRKDDTP LFLNDTSAFD FSDEVSDEGL SRFNKLRVVV
60 70 80 90 100
ADDDSEAPER PVNGAHPALQ ADDDSLLDQD LPLTNSQLSL KMDPCDNCSK
110 120 130 140 150
RRELLKQRKV KTRLTIAAVL YLLFMIGELV GGYMANSLAI MTDALHMLTD
160 170 180 190 200
LSAIILTLLA LWLSSKSPTR RFTFGFHRLE VLSAMISVML VYVLMGFLLY
210 220 230 240 250
EAVQRTIHMN YEINGDVMLI TAAVGVAVNV IMGFLLNQSG HHHSHAHSHS
260 270 280 290 300
LPSNSPSMVS SGHNHGQDSL AVRAAFVHAL GDLVQSVGVL IAAYIIRFKP
310 320 330 340 350
EYKIADPICT YIFSLLVAFT TFRIIWDTVV IILEGVPSHL NVDYIKESLM
360 370 380 390 400
KIEDVYSVED LNIWSLTSGK STAIVHMQLI PGSSSKWEEV QSKAKHLLLN
410 420 430
TFGMYKCTIQ LQSYRQEVIR TCANCHSSST
Length:430
Mass (Da):47,800
Last modified:July 27, 2011 - v2
Checksum:iE2311FA6316D15C6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti426 – 4261H → Q in AAB82593 (PubMed:9354792).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004099 Genomic DNA. Translation: AAB82413.1.
AF003747 mRNA. Translation: AAB82593.1.
AF004098 Genomic DNA. Translation: AAB82412.1.
AF004097 Genomic DNA. Translation: AAB82411.1.
AF004100 Genomic DNA. Translation: AAB82414.1.
AK134245 mRNA. Translation: BAE22062.1.
AL772253 Genomic DNA. Translation: CAM13308.1.
CCDSiCCDS16667.1.
RefSeqiNP_001277922.1. NM_001290993.1.
NP_035904.2. NM_011774.3.
UniGeneiMm.27801.

Genome annotation databases

EnsembliENSMUST00000005952; ENSMUSP00000005952; ENSMUSG00000005802.
GeneIDi22785.
KEGGimmu:22785.
UCSCiuc008mba.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004099 Genomic DNA. Translation: AAB82413.1.
AF003747 mRNA. Translation: AAB82593.1.
AF004098 Genomic DNA. Translation: AAB82412.1.
AF004097 Genomic DNA. Translation: AAB82411.1.
AF004100 Genomic DNA. Translation: AAB82414.1.
AK134245 mRNA. Translation: BAE22062.1.
AL772253 Genomic DNA. Translation: CAM13308.1.
CCDSiCCDS16667.1.
RefSeqiNP_001277922.1. NM_001290993.1.
NP_035904.2. NM_011774.3.
UniGeneiMm.27801.

3D structure databases

ProteinModelPortaliO35149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35149. 1 interaction.
MINTiMINT-1605304.
STRINGi10090.ENSMUSP00000005952.

Protein family/group databases

TCDBi2.A.4.3.3. the cation diffusion facilitator (cdf) family.

PTM databases

PhosphoSiteiO35149.

Proteomic databases

PRIDEiO35149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005952; ENSMUSP00000005952; ENSMUSG00000005802.
GeneIDi22785.
KEGGimmu:22785.
UCSCiuc008mba.2. mouse.

Organism-specific databases

CTDi7782.
MGIiMGI:1345282. Slc30a4.

Phylogenomic databases

eggNOGiCOG1230.
GeneTreeiENSGT00550000074381.
HOGENOMiHOG000079023.
HOVERGENiHBG003345.
InParanoidiO35149.
KOiK14691.
OMAiGLNMDPC.
OrthoDBiEOG7S4X68.
TreeFamiTF313382.

Miscellaneous databases

NextBioi303359.
PROiO35149.
SOURCEiSearch...

Gene expression databases

BgeeiO35149.
ExpressionAtlasiO35149. baseline and differential.
GenevisibleiO35149. MM.

Family and domain databases

Gene3Di1.20.1510.10. 1 hit.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 1 hit.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel gene involved in zinc transport is deficient in the lethal milk mouse."
    Huang L., Gitschier J.
    Nat. Genet. 17:292-297(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: B6/CBAF1 and C57BL/6.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Forelimb.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiZNT4_MOUSE
AccessioniPrimary (citable) accession number: O35149
Secondary accession number(s): O35154, Q3UYZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.