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O35149 (ZNT4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc transporter 4

Short name=ZnT-4
Alternative name(s):
Lethal milk protein
Solute carrier family 30 member 4
Gene names
Name:Slc30a4
Synonyms:Lm, Znt4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probably involved in zinc transport out of the cytoplasm, may be by sequestration into an intracellular compartment.

Subcellular location

Endosome membrane; Multi-pass membrane protein Probable.

Tissue specificity

Widely expressed. Highly expressed in the brain and in mammary epithelial cell lines.

Domain

Contains a histidine-rich region which is a ligand for zinc and an aspartate-rich region which is a potential ligand for zinc By similarity.

Involvement in disease

Defects in Slc30a4 are the cause of the lethal milk (lm) phenotype. Mice with lm are defective in zinc transport into breast milk, due to a premature translation termination codon at position 297. Only homozygous mutant adults develop dermatitis, skin lesions, and hair loss due to a systemic zinc deficiency. However, neonatal mice (of any genotype) suckled on homozygous mutant female also develop symptoms characteristic of nutritional zinc deficiency, including dermatitis, alopecia and stunted growth.

Sequence similarities

Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 430430Zinc transporter 4
PRO_0000206100

Regions

Topological domain1 – 113113Cytoplasmic Potential
Transmembrane114 – 13421Helical; Potential
Topological domain135 – 1439Vacuolar Potential
Transmembrane144 – 16421Helical; Potential
Topological domain165 – 17814Cytoplasmic Potential
Transmembrane179 – 19921Helical; Potential
Topological domain200 – 21617Vacuolar Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 27538Cytoplasmic Potential
Transmembrane276 – 29621Helical; Potential
Topological domain297 – 31115Vacuolar Potential
Transmembrane312 – 33221Helical; Potential
Topological domain333 – 43098Cytoplasmic Potential
Compositional bias17 – 9680Asp-rich (acidic)
Compositional bias241 – 2499His-rich

Experimental info

Sequence conflict4261H → Q in AAB82593. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O35149 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: E2311FA6316D15C6

FASTA43047,800
        10         20         30         40         50         60 
MAGPGAWKRL KSLLRKDDTP LFLNDTSAFD FSDEVSDEGL SRFNKLRVVV ADDDSEAPER 

        70         80         90        100        110        120 
PVNGAHPALQ ADDDSLLDQD LPLTNSQLSL KMDPCDNCSK RRELLKQRKV KTRLTIAAVL 

       130        140        150        160        170        180 
YLLFMIGELV GGYMANSLAI MTDALHMLTD LSAIILTLLA LWLSSKSPTR RFTFGFHRLE 

       190        200        210        220        230        240 
VLSAMISVML VYVLMGFLLY EAVQRTIHMN YEINGDVMLI TAAVGVAVNV IMGFLLNQSG 

       250        260        270        280        290        300 
HHHSHAHSHS LPSNSPSMVS SGHNHGQDSL AVRAAFVHAL GDLVQSVGVL IAAYIIRFKP 

       310        320        330        340        350        360 
EYKIADPICT YIFSLLVAFT TFRIIWDTVV IILEGVPSHL NVDYIKESLM KIEDVYSVED 

       370        380        390        400        410        420 
LNIWSLTSGK STAIVHMQLI PGSSSKWEEV QSKAKHLLLN TFGMYKCTIQ LQSYRQEVIR 

       430 
TCANCHSSST 

« Hide

References

« Hide 'large scale' references
[1]"A novel gene involved in zinc transport is deficient in the lethal milk mouse."
Huang L., Gitschier J.
Nat. Genet. 17:292-297(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: B6/CBAF1 and C57BL/6.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Forelimb.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF004099 Genomic DNA. Translation: AAB82413.1.
AF003747 mRNA. Translation: AAB82593.1.
AF004098 Genomic DNA. Translation: AAB82412.1.
AF004097 Genomic DNA. Translation: AAB82411.1.
AF004100 Genomic DNA. Translation: AAB82414.1.
AK134245 mRNA. Translation: BAE22062.1.
AL772253 Genomic DNA. Translation: CAM13308.1.
RefSeqNP_035904.2. NM_011774.2.
UniGeneMm.27801.

3D structure databases

ProteinModelPortalO35149.
SMRO35149. Positions 115-209.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35149. 1 interaction.
MINTMINT-1605304.

Protein family/group databases

TCDB2.A.4.3.3. the cation diffusion facilitator (cdf) family.

PTM databases

PhosphoSiteO35149.

Proteomic databases

PRIDEO35149.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005952; ENSMUSP00000005952; ENSMUSG00000005802.
GeneID22785.
KEGGmmu:22785.
UCSCuc008mba.1. mouse.

Organism-specific databases

CTD7782.
MGIMGI:1345282. Slc30a4.

Phylogenomic databases

eggNOGCOG1230.
GeneTreeENSGT00550000074381.
HOGENOMHOG000079023.
HOVERGENHBG003345.
InParanoidQ3UYZ9.
KOK14691.
OMATAIVHMQ.
OrthoDBEOG7S4X68.
TreeFamTF313382.

Gene expression databases

ArrayExpressO35149.
BgeeO35149.
GenevestigatorO35149.

Family and domain databases

Gene3D1.20.1510.10. 1 hit.
InterProIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERPTHR11562. PTHR11562. 1 hit.
PfamPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsTIGR01297. CDF. 1 hit.
ProtoNetSearch...

Other

NextBio303359.
PROO35149.
SOURCESearch...

Entry information

Entry nameZNT4_MOUSE
AccessionPrimary (citable) accession number: O35149
Secondary accession number(s): O35154, Q3UYZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot