O35147 (BAD_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 99.
History...
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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Bcl2 antagonist of cell death Short name=BAD Alternative name(s): Bcl-2-binding component 6 Bcl-xL/Bcl-2-associated death promoter | ||
| Gene names |
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| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 205 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 By similarity. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Subunit structure | Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10. The Ser-113/Ser-137 phosphorylated form binds 14-3-3 proteins. Interacts with AKT1 and PIM3 By similarity. |
| Subcellular location | Mitochondrion outer membrane By similarity. Cytoplasm By similarity. Note: Upon phosphorylation, locates to the cytoplasm By similarity. |
| Tissue specificity | Expressed in all tissues tested, including brain, liver, spleen and heart. In the brain, restricted to epithelial cells of the choroid plexus. Isoform alpha is the more abundant form. |
| Domain | Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. |
| Post-translational modification | Phosphorylated at one or more of Ser-113, Ser-137, Ser-156 and Ser-171 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-137 or Ser-113 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-156, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-137 is the major site of AKT/PKB phosphorylation, Ser-156 the major site of protein kinase A (CAPK) phosphorylation. Methylation at Arg-132 and Arg-134 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-137 By similarity. |
| Sequence similarities | Belongs to the Bcl-2 family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Alpha (identifier: O35147-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Beta (identifier: O35147-2) The sequence of this isoform differs from the canonical sequence as follows: 166-205: LPRPKSAGTA...LGKGGSTPSQ → EELTYSVEFL...RYWTALRRLC |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 205 | 205 | Bcl2 antagonist of cell death | PRO_0000143105 | |||||
Regions | |||||||||
| Motif | 148 – 162 | 15 | BH3 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 113 | 1 | Phosphoserine; by PKA, PKB, PIM2, PIM3, PAK1, PAK2, PAK4, PAK7/PAK5, RPS6KA1 and RAF1 By similarity | ||||||
| Modified residue | 129 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 132 | 1 | Asymmetric dimethylarginine; by PRMT1 By similarity | ||||||
| Modified residue | 134 | 1 | Asymmetric dimethylarginine; by PRMT1 By similarity | ||||||
| Modified residue | 137 | 1 | Phosphoserine; by PKA, PKB, PAK1, RPS6KA1, RPS6KB1 and PKC/PRKCQ By similarity | ||||||
| Modified residue | 156 | 1 | Phosphoserine; by PKA and PKB By similarity | ||||||
| Modified residue | 171 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 166 – 205 | 40 | LPRPK…STPSQ → EELTYSVEFLPVRAIAMEGW PLLWSFQSFPHTLPPTPPEV AMFPLRYWTALRRLC in isoform Beta. | VSP_000534 | |||||
Experimental info | |||||||||
| Mutagenesis | 113 | 1 | S → A: No effect on heterodimerization with 14-3-3 proteins. Ref.1 | ||||||
| Mutagenesis | 137 | 1 | S → A: No heterodimerization with 14-3-3 proteins. No effect on heterodimerization with BCL2 nor with protein P11. Ref.1 | ||||||
| Sequence conflict | 29 – 34 | 6 | SDAGGR → ERRGRK in AAC53374. Ref.1 | ||||||
Sequences
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References
| [1] | "Interference of BAD (Bcl-xL/Bcl-2-associated death promoter)-induced apoptosis in mammalian cells by 14-3-3 isoforms and P11." Hsu S.Y., Kaipia A., Zhu L., Hsueh A.J.W. Mol. Endocrinol. 11:1858-1867(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF SER-113 AND SER-137. Tissue: Ovary. |
| [2] | "Cloning and expression of the programmed cell death regulator BAD in the rat brain." D'Agata V., Magro G., Travali S., Musco S., Cavallaro S. Neurosci. Lett. 243:137-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [3] | "Functional characterization of two splice variants of rat BAD and their interaction with Bcl-w in sympathetic neurons." Hamner S., Arumae U., Yu L.-Y., Sun Y.-F., Saarma M., Lindholm D. Mol. Cell. Neurosci. 17:97-106(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). Tissue: Brain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF003523 mRNA. Translation: AAC53374.1. AF031227 mRNA. Translation: AAC15100.1. AF279910 mRNA. Translation: AAF91427.1. AF279911 mRNA. Translation: AAF91428.1. |
| IPI | IPI00206258. IPI00231724. |
| RefSeq | NP_073189.1. NM_022698.1. |
| UniGene | Rn.36696. |
3D structure databases | |
| ProteinModelPortal | O35147. |
| SMR | O35147. Positions 138-164. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-29862N. |
PTM databases | |
| PhosphoSite | O35147. |
Proteomic databases | |
| PaxDb | O35147. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOT00000028712; ENSRNOP00000028712; ENSRNOG00000021147. ENSRNOT00000067068; ENSRNOP00000061855; ENSRNOG00000021147. |
| GeneID | 64639. |
| KEGG | rno:64639. |
| UCSC | RGD:620103. rat. |
Organism-specific databases | |
| CTD | 572. |
| RGD | 620103. Bad. |
Phylogenomic databases | |
| eggNOG | NOG43412. |
| GeneTree | ENSGT00390000010740. |
| HOGENOM | HOG000095169. |
| HOVERGEN | HBG001653. |
| InParanoid | O35147. |
| KO | K02158. |
| OMA | SMFQIPE. |
Gene expression databases | |
| Genevestigator | O35147. |
| GermOnline | ENSRNOG00000021147. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR018868. Bcl-2_BAD. [Graphical view] |
| Pfam | PF10514. Bcl-2_BAD. 1 hit. [Graphical view] |
| PROSITE | PS01259. BH3. False negative. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 613624. |
Entry information
| Entry name | BAD_RAT | ||||||||
| Accession | Primary (citable) accession number: O35147 Secondary accession number(s): O70256, Q9JHX1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |