Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras association domain-containing protein 5

Gene

Rassf5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri117 – 16549Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 5
Alternative name(s):
Maxp1
New ras effector 1
Gene namesi
Name:Rassf5
Synonyms:Nore1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi621694. Rassf5.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: Mainly located in the perinuclear region of unstimulated primary T-cells; upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Ras association domain-containing protein 5PRO_0000240403Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei177 – 1771PhosphoserineCombined sources
Modified residuei347 – 3471PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35141.
PRIDEiO35141.

Expressioni

Gene expression databases

ExpressionAtlasiO35141. baseline and differential.
GenevisibleiO35141. RN.

Interactioni

Subunit structurei

Interacts directly with activated HRAS; a RASSF5-STK4/MST1 complex probably associates with activated HRAS. Interacts with KRAS. Probably interacts with Ras-like GTPases RRAS, RRAS2, MRAS, RAP1B, RAP2A and RALA. Can self-associate. Interacts with RSSF1 isoform A. The RSSF1 isoform A-RSSF5 heterodimer probably mediates the association of RSSF1 with HRAS. Interacts with activated RAP1A and ITGAL/LFA-1. Binds STK4/MST1, inhibiting STK4/MST1 autoactivation (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008055.

Structurei

3D structure databases

ProteinModelPortaliO35141.
SMRiO35141. Positions 108-166, 200-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini265 – 35995Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini361 – 40848SARAHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SARAH domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri117 – 16549Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IPAE. Eukaryota.
ENOG4111HS2. LUCA.
GeneTreeiENSGT00390000003367.
HOGENOMiHOG000013025.
HOVERGENiHBG054362.
InParanoidiO35141.
KOiK08015.
OMAiRGWCDLC.
OrthoDBiEOG7TF796.
PhylomeDBiO35141.
TreeFamiTF319243.

Family and domain databases

InterProiIPR002219. PE/DAG-bd.
IPR000159. RA_dom.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF16517. Nore1-SARAH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPAIGQRP YPLLLDPEPP RYLQSLGGTE PPPPARPRRC IPTALISASG
60 70 80 90 100
ASEGRGSRRN ARGDPEPTPR DCRHARPVRP GLQQRLRRRP GSHRPRDVRS
110 120 130 140 150
IFEQPQDPRV LAERGEGHRF AELALRGGPG WCDLCGREVL RQALRCANCK
160 170 180 190 200
FTCHPECRSL IQLDCRQKEG PALDRQSPES TLTPTFNKNV CKAVEETQHP
210 220 230 240 250
PTIQEIKQKI DSYNSREKHC LGMKLSEDGT YTGFIKVHLK LRRPVTVPAG
260 270 280 290 300
IRPQSIYDAI KEVNPAATTD KRTSFYLPLD AIKQLHISSS TTVSEVIQGL
310 320 330 340 350
LKKFMVVDNP QKFALFKRIH KDGQVLFQKL SIADCPLYLR LLAGPDTDVL
360 370 380 390 400
SFVLKENETG DVEWDAFSIP ELQNFLTILE KEEQDKIHQL QKKYNKFRQK
410
LEEALRESQG KPG
Length:413
Mass (Da):46,680
Last modified:January 1, 1998 - v1
Checksum:i4A44E5830D9F094D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002251 mRNA. Translation: AAB71821.1.
RefSeqiNP_062238.1. NM_019365.3.
UniGeneiRn.36106.

Genome annotation databases

EnsembliENSRNOT00000008055; ENSRNOP00000008055; ENSRNOG00000005342.
GeneIDi54355.
KEGGirno:54355.
UCSCiRGD:621694. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002251 mRNA. Translation: AAB71821.1.
RefSeqiNP_062238.1. NM_019365.3.
UniGeneiRn.36106.

3D structure databases

ProteinModelPortaliO35141.
SMRiO35141. Positions 108-166, 200-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008055.

Proteomic databases

PaxDbiO35141.
PRIDEiO35141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008055; ENSRNOP00000008055; ENSRNOG00000005342.
GeneIDi54355.
KEGGirno:54355.
UCSCiRGD:621694. rat.

Organism-specific databases

CTDi83593.
RGDi621694. Rassf5.

Phylogenomic databases

eggNOGiENOG410IPAE. Eukaryota.
ENOG4111HS2. LUCA.
GeneTreeiENSGT00390000003367.
HOGENOMiHOG000013025.
HOVERGENiHBG054362.
InParanoidiO35141.
KOiK08015.
OMAiRGWCDLC.
OrthoDBiEOG7TF796.
PhylomeDBiO35141.
TreeFamiTF319243.

Miscellaneous databases

PROiO35141.

Gene expression databases

ExpressionAtlasiO35141. baseline and differential.
GenevisibleiO35141. RN.

Family and domain databases

InterProiIPR002219. PE/DAG-bd.
IPR000159. RA_dom.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF16517. Nore1-SARAH. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Maxp1, protein interacting with guanine nucleotide exchange factor Mss4."
    Slepnev V.I., De Camilli P.V.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The pro-apoptotic Ras effector Nore1 may serve as a Ras-regulated tumor suppressor in the lung."
    Vos M.D., Martinez A., Ellis C.A., Vallecorsa T., Clark G.J.
    J. Biol. Chem. 278:21938-21943(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HRAS.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRASF5_RAT
AccessioniPrimary (citable) accession number: O35141
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: January 1, 1998
Last modified: February 17, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.