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Protein

DNA-directed RNA polymerase I subunit RPA1

Gene

Polr1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641ZincBy similarity
Metal bindingi67 – 671ZincBy similarity
Metal bindingi74 – 741ZincBy similarity
Metal bindingi77 – 771ZincBy similarity
Metal bindingi595 – 5951Magnesium; catalyticBy similarity
Metal bindingi597 – 5971Magnesium; catalyticBy similarity
Metal bindingi599 – 5991Magnesium; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73863. RNA Polymerase I Transcription Termination.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA1 (EC:2.7.7.6)
Short name:
RNA polymerase I subunit A1
Alternative name(s):
DNA-directed RNA polymerase I largest subunit
DNA-directed RNA polymerase I subunit A
RNA polymerase I 194 kDa subunit
Short name:
RPA194
Gene namesi
Name:Polr1a
Synonyms:Rpa1, Rpo1-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1096397. Polr1a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • DNA-directed RNA polymerase I complex Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17171717DNA-directed RNA polymerase I subunit RPA1PRO_0000073924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1393 – 13931PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35134.
MaxQBiO35134.
PaxDbiO35134.
PRIDEiO35134.

PTM databases

iPTMnetiO35134.
PhosphoSiteiO35134.

Expressioni

Gene expression databases

BgeeiO35134.
CleanExiMM_RPA1.
GenevisibleiO35134. MM.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. The RNA polymerase complex associates with several transcription factors to form the respective holoenzyme complex (By similarity). Interacts with MYO1C. Interacts with ERBB2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi202995. 8 interactions.
STRINGi10090.ENSMUSP00000060858.

Structurei

3D structure databases

ProteinModelPortaliO35134.
SMRiO35134. Positions 8-110, 261-1031, 1196-1289, 1544-1716.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni968 – 98013Bridging helixBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiKOG0262. Eukaryota.
COG0086. LUCA.
GeneTreeiENSGT00550000074861.
HOGENOMiHOG000205401.
HOVERGENiHBG017741.
InParanoidiO35134.
KOiK02999.
OMAiMGSYGRC.
OrthoDBiEOG7H1JJM.
PhylomeDBiO35134.
TreeFamiTF103033.

Family and domain databases

InterProiIPR015699. DNA-dir_RNA_pol1_lsu.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PANTHERiPTHR19376:SF11. PTHR19376:SF11. 4 hits.
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASKHTPWR RLQGISFGMY SAEELKKLSV KSITNPRYVD YLGNPSANGL
60 70 80 90 100
YDLALGPADS KEVCATCVQD FNNCSGHLGH IDLPLTVYNP FLFDKLYLLL
110 120 130 140 150
RGSCLSCHML TCPRAAIYLL ISQLRVLEVG ALQAVYELER ILSRFLEETG
160 170 180 190 200
DPSAFEIQEE LEEYTSKILQ NNLLGSQGTH VKNVCESRSK LVAQFWKTHM
210 220 230 240 250
AAKQCPHCKT GRSVVRKEHN SKLIITYPAT VHKKSDQEGT ELPEGVPEAP
260 270 280 290 300
GIDKAQMGKR GYLTPSSAQE HLFAIWKNEG FFLNYLFSGL DDIGPESSFN
310 320 330 340 350
PSMFFLDFIV VPPSRYRPVN RLGDQMFTNG QTVNLQAVMK DAVLIRKLLA
360 370 380 390 400
LMAQEQKLPC EMTELTIDKE NDSSVAIDRS FLGLLPGPSL TDKLYNIWIR
410 420 430 440 450
LQSHVNIVFD SEMDKLMLEK YPGIRQILEK KEGLFRKHMM GKRVDYAARS
460 470 480 490 500
VICPDMYINT NEIGIPMVFA TKLTYPQPVT PWNVQELRQA VINGPNVHPG
510 520 530 540 550
ASMVINEDGS RTALSSVDAA QREAVAKQLL TPATGAPKPQ GTKVVCRHVK
560 570 580 590 600
NGDILLLNRQ PTLHRPSIQA HRARILPEEK VLRLHYANCK AYNADFDGDE
610 620 630 640 650
MNAHFPQSEL GRAEAYVLAC TDQQYLVPKD GQPLAGLIQD HMVSGANMTI
660 670 680 690 700
RGCFFTREQY MELVYRGLTD KVGRVKLFPP AILKPFPLWT GKQVVSTLLI
710 720 730 740 750
NIIPEDYAPL NLSGKAKIGS KAWVKEKPRP IPDFDPDSMC ESQVIIREGE
760 770 780 790 800
LLCGVLDKAH YGSSAYGLVH CCYEIYGGET SGRVLTCLAR LFTAYLQLYR
810 820 830 840 850
GFTLGVEDIL VKPNADVVRQ RIIEESTQCG PQAVKAALSL PETASCDEIQ
860 870 880 890 900
GKWQDAHLSK DQRDFNMIDM KFKEEVNHYS NEINKACMPL GLHRQFPENN
910 920 930 940 950
LQMMVQSGAK GSTVNTMQIS CLLGQIELEG RRPPLMASGK SLPCFEPYEF
960 970 980 990 1000
TPRAGGFVTG RFLTGIRPPE FFFHCMAGRE GLVDTAVKTS RSGYLQRCII
1010 1020 1030 1040 1050
KHLEGLVIQY DLTVRDSDGS VVQFLYGEDG LDIPKTQFLQ PKQFPFLAGN
1060 1070 1080 1090 1100
YEVIMKSKHL HEVLSRADPQ KVLGHIKAIK KWHHKHSGAL LRKGAFLSFS
1110 1120 1130 1140 1150
QKIQAAVKAL NLKGSIQNGR SPETQQMLQM WYDLDEESRW KYQKRAAPCP
1160 1170 1180 1190 1200
DPSLSVWRPD IYFASVSETF EKKIDDFSQE WAAQAERSYK KSELSLDRLR
1210 1220 1230 1240 1250
TLLQLKWQRS LCDPGEAVGL LAAQSIGEPS TQMTLNTFHF AGRGEMNVTL
1260 1270 1280 1290 1300
GIPRLREILM VASANIKTPM MSVPVFDTKK ALKKVKSLKK RLTRVCLGEV
1310 1320 1330 1340 1350
LQKVDIQESF CMGEKRNKFQ VYELRFQFLP HAYYQQEKCL RPEDILHFME
1360 1370 1380 1390 1400
TRFFKLLMEA IKKKKNKASA FRNVNSRRAT QKDLNDTEDS GRSQREEERD
1410 1420 1430 1440 1450
EEEEGNIVDA EAEEGDADAS DTKRKEKQEE EVDYESEEEG EEEEEEEVQE
1460 1470 1480 1490 1500
EGNIKGDGVH QGHEPDEEEH LGLEEEESSQ KPPRRHSRPQ GAEAIKRRIQ
1510 1520 1530 1540 1550
AVRESYSFIE DYQYDTEESL WCQVTVKLPL MKINFDMSSL VVSLAHKAIV
1560 1570 1580 1590 1600
YTTKGITRCL LNETTNSKNE KELVLNTEGI NLPELFKYSE ILDLRRLYSN
1610 1620 1630 1640 1650
DIHAMANTYG IEAALRVIEK EIKDVFAVYG IAVDPRHLSL VADYMCFEGV
1660 1670 1680 1690 1700
YKPLNRFGIQ SSSSPLQQMT FETSFQFLKQ ATMMGSHDEL KSPSACLVVG
1710
KVVKGGTGLF ELKQPLR
Length:1,717
Mass (Da):194,110
Last modified:December 7, 2004 - v2
Checksum:iE78B7E3F6F7129F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti821 – 8211R → H in AAH53744 (PubMed:15489334).Curated
Sequence conflicti1246 – 12461M → T in AAB66718 (PubMed:9236775).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000938 mRNA. Translation: AAB66718.1.
BC053744 mRNA. Translation: AAH53744.1.
CCDSiCCDS20236.1.
PIRiT13961.
RefSeqiNP_033114.3. NM_009088.3.
UniGeneiMm.135581.

Genome annotation databases

EnsembliENSMUST00000055296; ENSMUSP00000060858; ENSMUSG00000049553.
GeneIDi20019.
KEGGimmu:20019.
UCSCiuc009chv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000938 mRNA. Translation: AAB66718.1.
BC053744 mRNA. Translation: AAH53744.1.
CCDSiCCDS20236.1.
PIRiT13961.
RefSeqiNP_033114.3. NM_009088.3.
UniGeneiMm.135581.

3D structure databases

ProteinModelPortaliO35134.
SMRiO35134. Positions 8-110, 261-1031, 1196-1289, 1544-1716.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202995. 8 interactions.
STRINGi10090.ENSMUSP00000060858.

PTM databases

iPTMnetiO35134.
PhosphoSiteiO35134.

Proteomic databases

EPDiO35134.
MaxQBiO35134.
PaxDbiO35134.
PRIDEiO35134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055296; ENSMUSP00000060858; ENSMUSG00000049553.
GeneIDi20019.
KEGGimmu:20019.
UCSCiuc009chv.2. mouse.

Organism-specific databases

CTDi25885.
MGIiMGI:1096397. Polr1a.

Phylogenomic databases

eggNOGiKOG0262. Eukaryota.
COG0086. LUCA.
GeneTreeiENSGT00550000074861.
HOGENOMiHOG000205401.
HOVERGENiHBG017741.
InParanoidiO35134.
KOiK02999.
OMAiMGSYGRC.
OrthoDBiEOG7H1JJM.
PhylomeDBiO35134.
TreeFamiTF103033.

Enzyme and pathway databases

ReactomeiR-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73863. RNA Polymerase I Transcription Termination.

Miscellaneous databases

ChiTaRSiPolr1a. mouse.
PROiO35134.
SOURCEiSearch...

Gene expression databases

BgeeiO35134.
CleanExiMM_RPA1.
GenevisibleiO35134. MM.

Family and domain databases

InterProiIPR015699. DNA-dir_RNA_pol1_lsu.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PANTHERiPTHR19376:SF11. PTHR19376:SF11. 4 hits.
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the cDNA encoding the largest subunit of mouse RNA polymerase I."
    Seither P., Coy J.F., Pouska A., Grummt I.
    Mol. Gen. Genet. 255:180-186(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Limb.
  3. "The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription."
    Percipalle P., Fomproix N., Cavellan E., Voit R., Reimer G., Krueger T., Thyberg J., Scheer U., Grummt I., Oestlund Farrants A.-K.O.
    EMBO Rep. 7:525-530(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYO1C.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Pancreas, Spleen and Testis.

Entry informationi

Entry nameiRPA1_MOUSE
AccessioniPrimary (citable) accession number: O35134
Secondary accession number(s): Q7TSA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.