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Protein

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Gene

Cyp27b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.

Catalytic activityi

Calcidiol + NADPH + O2 = calcitriol + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi448 – 4481Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • calcidiol 1-monooxygenase activity Source: BHF-UCL
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • aging Source: RGD
  • calcitriol biosynthetic process from calciol Source: GOC
  • lactation Source: RGD
  • response to calcium ion Source: RGD
  • response to cAMP Source: RGD
  • response to copper ion Source: RGD
  • response to drug Source: RGD
  • response to insulin Source: RGD
  • response to peptide hormone Source: RGD
  • response to prostaglandin E Source: RGD
  • response to vitamin D Source: RGD
  • vitamin D catabolic process Source: BHF-UCL
  • vitamin D metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14354.
UniPathwayiUPA00955.

Names & Taxonomyi

Protein namesi
Recommended name:
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (EC:1.14.13.13)
Alternative name(s):
25-OHD-1 alpha-hydroxylase
25-hydroxyvitamin D(3) 1-alpha-hydroxylase
Short name:
VD3 1A hydroxylase
Calcidiol 1-monooxygenase
Cytochrome P450 subfamily XXVIIB polypeptide 1
Cytochrome P450C1 alpha
Cytochrome P450VD1-alpha
Cytochrome p450 27B1
Gene namesi
Name:Cyp27b1
Synonyms:Cyp27b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69192. Cyp27b1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 50125-hydroxyvitamin D-1 alpha hydroxylase, mitochondrialPRO_0000003624
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

PRIDEiO35132.

Expressioni

Tissue specificityi

Kidney.

Gene expression databases

GenevestigatoriO35132.

Interactioni

Protein-protein interaction databases

BioGridi250405. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO35132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG106909.
InParanoidiO35132.
KOiK07438.
PhylomeDBiO35132.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQAVKLASR VFHRVQLPSQ LGSDSVLRSL SDIPGPSTPS FLAELFCKGG
60 70 80 90 100
LSRLHELQVH GAARYGPIWS GSFGTLRTVY VADPALVEQL LRQESHCPER
110 120 130 140 150
CSFSSWSEHR RRHQRACGLL TADGEEWQRL RSLLAPLLLR PQAAAGYAGT
160 170 180 190 200
LDSVVSDLVR RLRRQRGRGS GLPDLVLDVA GEFYKFGLEG IGAVLLGSRL
210 220 230 240 250
GCLEAEVPPD TETFIEAVGS VFVSTLLTMA MPSWLHRLIP GPWARLCRDW
260 270 280 290 300
DQMFAFAQKH VEQREGEAAV RNQGKPEEDL PTGHHLTHFL FREKVSVQSI
310 320 330 340 350
VGNVTELLLA GVDTVSNTLS WALYELSRHP EVQSALHSEI TGAVNPGSYA
360 370 380 390 400
HLQATALSQL PLLKAVIKEV LRLYPVVPGN SRVPDRDICV GNYVIPQDTL
410 420 430 440 450
VSLCHYATSR DPAQFREPNS FNPARWLGEG PAPHPFASLP FGFGKRSCIG
460 470 480 490 500
RRLAELELQM ALAQILTHFE VLPEPGALPV KPMTRTVLVP ERSIHLQFVD

R
Length:501
Mass (Da):55,369
Last modified:July 15, 1998 - v2
Checksum:iB0A85286A219EA0E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131H → D in AAB86461 (PubMed:9333115).Curated
Sequence conflicti55 – 551H → D in AAB86461 (PubMed:9333115).Curated
Sequence conflicti103 – 11210FSSWSEHRRR → SHLGQSTVAS in AAB86461 (PubMed:9333115).Curated
Sequence conflicti119 – 1191L → W in AAB86461 (PubMed:9333115).Curated
Sequence conflicti129 – 14416RLRSL…RPQAA → EAPKSPGPASPPTSSS in AAB86461 (PubMed:9333115).CuratedAdd
BLAST
Sequence conflicti201 – 2011G → R in AAB86461 (PubMed:9333115).Curated
Sequence conflicti251 – 2511D → N in AAB86461 (PubMed:9333115).Curated
Sequence conflicti288 – 2881H → D in AAB86461 (PubMed:9333115).Curated
Sequence conflicti305 – 3051T → R in AAB86461 (PubMed:9333115).Curated
Sequence conflicti372 – 3743RLY → MLD in AAB86461 (PubMed:9333115).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000139 mRNA. Translation: AAB86461.1.
AB001992 mRNA. Translation: BAA23271.1.
RefSeqiNP_446215.1. NM_053763.1.
UniGeneiRn.10847.

Genome annotation databases

GeneIDi114700.
KEGGirno:114700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000139 mRNA. Translation: AAB86461.1.
AB001992 mRNA. Translation: BAA23271.1.
RefSeqiNP_446215.1. NM_053763.1.
UniGeneiRn.10847.

3D structure databases

ProteinModelPortaliO35132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250405. 1 interaction.

Proteomic databases

PRIDEiO35132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114700.
KEGGirno:114700.

Organism-specific databases

CTDi1594.
RGDi69192. Cyp27b1.

Phylogenomic databases

HOVERGENiHBG106909.
InParanoidiO35132.
KOiK07438.
PhylomeDBiO35132.

Enzyme and pathway databases

UniPathwayiUPA00955.
BioCyciMetaCyc:MONOMER-14354.

Miscellaneous databases

NextBioi618833.
PROiO35132.

Gene expression databases

GenevestigatoriO35132.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The 25-hydroxyvitamin D 1-alpha-hydroxylase gene maps to the pseudovitamin D-deficiency rickets (PDDR) disease locus."
    St Arnaud R., Messerlian S., Moir J.M., Omdahl J.L., Glorieux F.H.
    J. Bone Miner. Res. 12:1552-1559(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "Cloning and expression of rat 25-hydroxyvitamin D3-1alpha-hydroxylase cDNA."
    Shinki T., Shimada H., Wakino S., Anazawa H., Hayashi M., Saruta T., Deluca H.F., Suda T.
    Proc. Natl. Acad. Sci. U.S.A. 94:12920-12925(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiCP27B_RAT
AccessioniPrimary (citable) accession number: O35132
Secondary accession number(s): O35076
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 29, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.