Reviewed, UniProtKB/Swiss-Prot O35132 (CP27B_RAT)

Names and origin

Protein names

Recommended name:
    25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    EC=1.14.13.13
Alternative name(s):
    Cytochrome P450 subfamily XXVIIB polypeptide 1
    Cytochrome p450 27B1
    Calcidiol 1-monooxygenase
    25-OHD-1 alpha-hydroxylase
    25-hydroxyvitamin D(3) 1-alpha-hydroxylase
      Short name=VD3 1A hydroxylase
    P450C1 alpha
    P450VD1-alpha

Gene names

Name:	Cyp27b1
Synonyms:	Cyp27b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Catalytic activity	Calcidiol + NADPH + O2 = calcitriol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Hormone biosynthesis; cholecalciferol biosynthesis.
Subcellular location	Mitochondrion membrane.
Tissue specificity	Kidney.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW vitamin D catabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcidiol 1-monooxygenase activity Ref.1 Ref.2 Inferred from direct assay. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 501		25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial		PRO_0000003624
Sites
	Metal binding	448	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	13	1	H → D in AAB86461. Ref.1
	Sequence conflict	55	1	H → D in AAB86461. Ref.1
	Sequence conflict	103 – 112	10	FSSWSEHRRR → SHLGQSTVAS in AAB86461. Ref.1
	Sequence conflict	119	1	L → W in AAB86461. Ref.1
	Sequence conflict	129 – 144	16	RLRSL…RPQAA → EAPKSPGPASPPTSSS in AAB86461. Ref.1
	Sequence conflict	201	1	G → R in AAB86461. Ref.1
	Sequence conflict	251	1	D → N in AAB86461. Ref.1
	Sequence conflict	288	1	H → D in AAB86461. Ref.1
	Sequence conflict	305	1	T → R in AAB86461. Ref.1
	Sequence conflict	372 – 374	3	RLY → MLD in AAB86461. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O35132-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: B0A85286A219EA0E	FASTA	501	55,369
	10 20 30 40 50 60 MTQAVKLASR VFHRVQLPSQ LGSDSVLRSL SDIPGPSTPS FLAELFCKGG LSRLHELQVH 70 80 90 100 110 120 GAARYGPIWS GSFGTLRTVY VADPALVEQL LRQESHCPER CSFSSWSEHR RRHQRACGLL 130 140 150 160 170 180 TADGEEWQRL RSLLAPLLLR PQAAAGYAGT LDSVVSDLVR RLRRQRGRGS GLPDLVLDVA 190 200 210 220 230 240 GEFYKFGLEG IGAVLLGSRL GCLEAEVPPD TETFIEAVGS VFVSTLLTMA MPSWLHRLIP 250 260 270 280 290 300 GPWARLCRDW DQMFAFAQKH VEQREGEAAV RNQGKPEEDL PTGHHLTHFL FREKVSVQSI 310 320 330 340 350 360 VGNVTELLLA GVDTVSNTLS WALYELSRHP EVQSALHSEI TGAVNPGSYA HLQATALSQL 370 380 390 400 410 420 PLLKAVIKEV LRLYPVVPGN SRVPDRDICV GNYVIPQDTL VSLCHYATSR DPAQFREPNS 430 440 450 460 470 480 FNPARWLGEG PAPHPFASLP FGFGKRSCIG RRLAELELQM ALAQILTHFE VLPEPGALPV 490 500 KPMTRTVLVP ERSIHLQFVD R

References

[1]	"The 25-hydroxyvitamin D 1-alpha-hydroxylase gene maps to the pseudovitamin D-deficiency rickets (PDDR) disease locus." St Arnaud R., Messerlian S., Moir J.M., Omdahl J.L., Glorieux F.H. J. Bone Miner. Res. 12:1552-1559(1997) [PubMed: 9333115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"Cloning and expression of rat 25-hydroxyvitamin D3-1alpha-hydroxylase cDNA." Shinki T., Shimada H., Wakino S., Anazawa H., Hayashi M., Saruta T., Deluca H.F., Suda T. Proc. Natl. Acad. Sci. U.S.A. 94:12920-12925(1997) [PubMed: 9371776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF000139 mRNA. Translation: AAB86461.1. AB001992 mRNA. Translation: BAA23271.1.
IPI	IPI00206228.
RefSeq	NP_446215.1.
UniGene	Rn.10847
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Genome annotation databases
GeneID	114700.
KEGG	rno:114700.
Organism-specific databases
RGD	69192. Cyp27b1.
Phylogenomic databases
HOVERGEN	O35132.
Enzyme and pathway databases
BRENDA	1.14.13.13. 248.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618833.

Entry information

Entry name

CP27B_RAT

Accession

Primary (citable) accession number: O35132
Secondary accession number(s): O35076

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)