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O35129

- PHB2_MOUSE

UniProt

O35129 - PHB2_MOUSE

Protein

Prohibitin-2

Gene

Phb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.By similarity2 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. mammary gland alveolus development Source: MGI
    2. mammary gland branching involved in thelarche Source: MGI
    3. negative regulation of intracellular estrogen receptor signaling pathway Source: MGI
    4. negative regulation of mammary gland epithelial cell proliferation Source: MGI
    5. negative regulation of transcription, DNA-templated Source: UniProtKB
    6. regulation of branching involved in mammary gland duct morphogenesis Source: MGI
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Receptor, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prohibitin-2
    Alternative name(s):
    B-cell receptor-associated protein BAP37
    Repressor of estrogen receptor activity
    Gene namesi
    Name:Phb2By similarity
    Synonyms:BapImported, Bcap37Imported, ReaImported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:102520. Phb2.

    Subcellular locationi

    Mitochondrion inner membrane. Cytoplasm. Nucleus
    Note: Also cytoplasmic and nuclear.

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB
    2. mitochondrion Source: UniProtKB
    3. nuclear matrix Source: UniProtKB
    4. nucleus Source: UniProtKB
    5. protein complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 299298Prohibitin-2PRO_0000213885Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei128 – 1281PhosphotyrosineBy similarity
    Modified residuei147 – 1471N6-acetyllysine1 Publication
    Modified residuei200 – 2001N6-acetyllysine1 Publication
    Modified residuei236 – 2361N6-acetyllysine1 Publication
    Modified residuei250 – 2501N6-acetyllysineBy similarity
    Modified residuei262 – 2621N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO35129.
    PaxDbiO35129.
    PRIDEiO35129.

    2D gel databases

    REPRODUCTION-2DPAGEO35129.

    PTM databases

    PhosphoSiteiO35129.

    Expressioni

    Tissue specificityi

    Widely expressed in different tissues.3 Publications

    Developmental stagei

    Throughout gestation, highly expressed in brown fat, heart, liver, developing renal tubules and neurons, and detected at lower levels in tissues such as lung and exocrine pancreas.1 Publication

    Gene expression databases

    ArrayExpressiO35129.
    BgeeiO35129.
    CleanExiMM_PHB2.
    GenevestigatoriO35129.

    Interactioni

    Subunit structurei

    Interacts with ZNF703 By similarity. Interacts with STOML2 By similarity. Interacts with PHB, ESR1, HDAC1 and HDAC5.By similarity3 Publications

    Protein-protein interaction databases

    BioGridi198310. 11 interactions.
    IntActiO35129. 8 interactions.
    MINTiMINT-1841450.

    Structurei

    3D structure databases

    ProteinModelPortaliO35129.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni19 – 4931Necessary for transcriptional repressionBy similarityAdd
    BLAST
    Regioni150 – 17425Necessary for transcriptional repressionBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili190 – 23849Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prohibitin family.Sequence Analysis

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0330.
    HOGENOMiHOG000205692.
    HOVERGENiHBG004457.
    InParanoidiO35129.
    KOiK17081.
    OMAiIGGVKKE.
    OrthoDBiEOG7V4B04.
    PhylomeDBiO35129.
    TreeFamiTF354230.

    Family and domain databases

    InterProiIPR001107. Band_7.
    IPR000163. Prohibitin.
    [Graphical view]
    PANTHERiPTHR23222. PTHR23222. 1 hit.
    PfamiPF01145. Band_7. 1 hit.
    [Graphical view]
    PRINTSiPR00679. PROHIBITIN.
    SMARTiSM00244. PHB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35129-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQNLKDLAG RLPAGPRGMG TALKLLLGAG AVAYGVRESV FTVEGGHRAI    50
    FFNRIGGVQQ DTILAEGLHF RIPWFQYPII YDIRARPRKI SSPTGSKDLQ 100
    MVNISLRVLS RPNAQELPSM YQRLGLDYEE RVLPSIVNEV LKSVVAKFNA 150
    SQLITQRAQV SLLIRRELTE RAKDFSLILD DVAITELSFS REYTAAVEAK 200
    QVAQQEAQRA QFLVEKAKQE QRQKIVQAEG EAEAAKMLGE ALSKNPGYIK 250
    LRKIRAAQNI SKTIATSQNR IYLTADNLVL NLQDESFTRG SDSLIKGKK 299
    Length:299
    Mass (Da):33,296
    Last modified:January 1, 1998 - v1
    Checksum:iA887CC982BF85C80
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti67 – 682GL → F in CAA55350. (PubMed:8070406)Curated
    Sequence conflicti81 – 811Y → N in AAC36528. (PubMed:9798653)Curated
    Sequence conflicti112 – 1121P → L in AAC36528. (PubMed:9798653)Curated
    Sequence conflicti120 – 1201M → I in AAC36528. (PubMed:9798653)Curated
    Sequence conflicti127 – 1271D → G in AAC36528. (PubMed:9798653)Curated
    Sequence conflicti138 – 1381N → S in AAC36528. (PubMed:9798653)Curated
    Sequence conflicti148 – 1481F → V in AAC36528. (PubMed:9798653)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78683 mRNA. Translation: CAA55350.1.
    AY319418 mRNA. Translation: AAP86652.1.
    AY211613 mRNA. Translation: AAP47231.1.
    AC002397 Genomic DNA. Translation: AAC36005.1.
    U89422 mRNA. Translation: AAC36528.1.
    CCDSiCCDS39627.1.
    PIRiS46996.
    RefSeqiNP_031557.2. NM_007531.2.
    UniGeneiMm.36241.

    Genome annotation databases

    EnsembliENSMUST00000004375; ENSMUSP00000004375; ENSMUSG00000004264.
    GeneIDi12034.
    KEGGimmu:12034.
    UCSCiuc009dri.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78683 mRNA. Translation: CAA55350.1 .
    AY319418 mRNA. Translation: AAP86652.1 .
    AY211613 mRNA. Translation: AAP47231.1 .
    AC002397 Genomic DNA. Translation: AAC36005.1 .
    U89422 mRNA. Translation: AAC36528.1 .
    CCDSi CCDS39627.1.
    PIRi S46996.
    RefSeqi NP_031557.2. NM_007531.2.
    UniGenei Mm.36241.

    3D structure databases

    ProteinModelPortali O35129.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198310. 11 interactions.
    IntActi O35129. 8 interactions.
    MINTi MINT-1841450.

    PTM databases

    PhosphoSitei O35129.

    2D gel databases

    REPRODUCTION-2DPAGE O35129.

    Proteomic databases

    MaxQBi O35129.
    PaxDbi O35129.
    PRIDEi O35129.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004375 ; ENSMUSP00000004375 ; ENSMUSG00000004264 .
    GeneIDi 12034.
    KEGGi mmu:12034.
    UCSCi uc009dri.1. mouse.

    Organism-specific databases

    CTDi 11331.
    MGIi MGI:102520. Phb2.

    Phylogenomic databases

    eggNOGi COG0330.
    HOGENOMi HOG000205692.
    HOVERGENi HBG004457.
    InParanoidi O35129.
    KOi K17081.
    OMAi IGGVKKE.
    OrthoDBi EOG7V4B04.
    PhylomeDBi O35129.
    TreeFami TF354230.

    Miscellaneous databases

    NextBioi 280279.
    PROi O35129.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35129.
    Bgeei O35129.
    CleanExi MM_PHB2.
    Genevestigatori O35129.

    Family and domain databases

    InterProi IPR001107. Band_7.
    IPR000163. Prohibitin.
    [Graphical view ]
    PANTHERi PTHR23222. PTHR23222. 1 hit.
    Pfami PF01145. Band_7. 1 hit.
    [Graphical view ]
    PRINTSi PR00679. PROHIBITIN.
    SMARTi SM00244. PHB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein."
      Terashima M., Kim K.-M., Adachi T., Nielsen P.J., Reth M., Koehler G., Lamers M.C.
      EMBO J. 13:3782-3792(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: BALB/cImported.
      Tissue: Lymphoid tissue.
    2. "Truncated estrogen receptor product-1 stimulates estrogen receptor alpha transcriptional activity by titration of repressor proteins."
      Lin V.Y., Resnick E.M., Shupnik M.A.
      J. Biol. Chem. 278:38125-38131(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ESR1.
      Strain: 129/SvJImported.
      Tissue: PituitaryImported.
    3. "Transcriptional regulation by the repressor of estrogen receptor activity via recruitment of histone deacetylases."
      Kurtev V., Margueron R., Kroboth K., Ogris E., Cavailles V., Seiser C.
      J. Biol. Chem. 279:24834-24843(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ESR1; HDAC1 AND HDAC5, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: BALB/cImported.
      Tissue: BrainImported.
    4. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
      Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
      Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 25-48; 55-71; 98-142; 148-165; 335-346; 866-885 AND 1051-1058, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    6. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
      Chu C.C., Paul W.E.
      Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-154.
      Strain: BALB/cImported.
      Tissue: SpleenImported.
    7. "Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence."
      Coates P.J., Nenutil R., McGregor A., Picksley S.M., Crouch D.H., Hall P.A., Wright E.G.
      Exp. Cell Res. 265:262-273(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PHB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    8. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-147; LYS-200; LYS-236 AND LYS-262, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiPHB2_MOUSE
    AccessioniPrimary (citable) accession number: O35129
    Secondary accession number(s): O89075, Q61336
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3