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Protein

Prohibitin-2

Gene

Phb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin-2
Alternative name(s):
B-cell receptor-associated protein BAP37
Repressor of estrogen receptor activity
Gene namesi
Name:Phb2By similarity
Synonyms:BapImported, Bcap37Imported, ReaImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:102520. Phb2.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: MGI
  • cell surface Source: UniProtKB
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • mitochondrial inner membrane Source: UniProtKB
  • mitochondrial outer membrane Source: MGI
  • mitochondrion Source: UniProtKB
  • nuclear matrix Source: UniProtKB
  • nucleus Source: UniProtKB
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002138852 – 299Prohibitin-2Add BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei128PhosphotyrosineBy similarity1
Modified residuei147N6-acetyllysineCombined sources1
Modified residuei151PhosphoserineBy similarity1
Modified residuei200N6-acetyllysineCombined sources1
Modified residuei236N6-acetyllysineCombined sources1
Modified residuei250N6-acetyllysineBy similarity1
Modified residuei262N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35129.
PaxDbiO35129.
PeptideAtlasiO35129.
PRIDEiO35129.
TopDownProteomicsiO35129.

2D gel databases

REPRODUCTION-2DPAGEO35129.

PTM databases

iPTMnetiO35129.
PhosphoSitePlusiO35129.
SwissPalmiO35129.

Expressioni

Tissue specificityi

Widely expressed in different tissues.3 Publications

Developmental stagei

Throughout gestation, highly expressed in brown fat, heart, liver, developing renal tubules and neurons, and detected at lower levels in tissues such as lung and exocrine pancreas.1 Publication

Gene expression databases

BgeeiENSMUSG00000004264.
CleanExiMM_PHB2.
ExpressionAtlasiO35129. baseline and differential.
GenevisibleiO35129. MM.

Interactioni

Subunit structurei

Interacts with PHB (PubMed:11302691). Interacts with ESR1, HDAC1 and HDAC5 (PubMed:12878603, PubMed:15140878). Interacts with ZNF703 (By similarity). Interacts with STOML2 (By similarity). Interacts with ARFGEF3 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rab32Q9CZE34EBI-445002,EBI-11566047

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198310. 12 interactors.
IntActiO35129. 12 interactors.
MINTiMINT-1841450.
STRINGi10090.ENSMUSP00000004375.

Structurei

3D structure databases

ProteinModelPortaliO35129.
SMRiO35129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 49Necessary for transcriptional repressionBy similarityAdd BLAST31
Regioni150 – 174Necessary for transcriptional repressionBy similarityAdd BLAST25

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili190 – 238Sequence analysisAdd BLAST49

Sequence similaritiesi

Belongs to the prohibitin family.Sequence analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3090. Eukaryota.
COG0330. LUCA.
GeneTreeiENSGT00550000075076.
HOGENOMiHOG000205692.
HOVERGENiHBG004457.
InParanoidiO35129.
KOiK17081.
OMAiSMAERMM.
OrthoDBiEOG091G0IHW.
PhylomeDBiO35129.
TreeFamiTF354230.

Family and domain databases

CDDicd03401. SPFH_prohibitin. 1 hit.
InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQNLKDLAG RLPAGPRGMG TALKLLLGAG AVAYGVRESV FTVEGGHRAI
60 70 80 90 100
FFNRIGGVQQ DTILAEGLHF RIPWFQYPII YDIRARPRKI SSPTGSKDLQ
110 120 130 140 150
MVNISLRVLS RPNAQELPSM YQRLGLDYEE RVLPSIVNEV LKSVVAKFNA
160 170 180 190 200
SQLITQRAQV SLLIRRELTE RAKDFSLILD DVAITELSFS REYTAAVEAK
210 220 230 240 250
QVAQQEAQRA QFLVEKAKQE QRQKIVQAEG EAEAAKMLGE ALSKNPGYIK
260 270 280 290
LRKIRAAQNI SKTIATSQNR IYLTADNLVL NLQDESFTRG SDSLIKGKK
Length:299
Mass (Da):33,296
Last modified:January 1, 1998 - v1
Checksum:iA887CC982BF85C80
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67 – 68GL → F in CAA55350 (PubMed:8070406).Curated2
Sequence conflicti81Y → N in AAC36528 (PubMed:9798653).Curated1
Sequence conflicti112P → L in AAC36528 (PubMed:9798653).Curated1
Sequence conflicti120M → I in AAC36528 (PubMed:9798653).Curated1
Sequence conflicti127D → G in AAC36528 (PubMed:9798653).Curated1
Sequence conflicti138N → S in AAC36528 (PubMed:9798653).Curated1
Sequence conflicti148F → V in AAC36528 (PubMed:9798653).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78683 mRNA. Translation: CAA55350.1.
AY319418 mRNA. Translation: AAP86652.1.
AY211613 mRNA. Translation: AAP47231.1.
AC002397 Genomic DNA. Translation: AAC36005.1.
U89422 mRNA. Translation: AAC36528.1.
CCDSiCCDS39627.1.
PIRiS46996.
RefSeqiNP_031557.2. NM_007531.2.
UniGeneiMm.36241.

Genome annotation databases

EnsembliENSMUST00000004375; ENSMUSP00000004375; ENSMUSG00000004264.
GeneIDi12034.
KEGGimmu:12034.
UCSCiuc033iuq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78683 mRNA. Translation: CAA55350.1.
AY319418 mRNA. Translation: AAP86652.1.
AY211613 mRNA. Translation: AAP47231.1.
AC002397 Genomic DNA. Translation: AAC36005.1.
U89422 mRNA. Translation: AAC36528.1.
CCDSiCCDS39627.1.
PIRiS46996.
RefSeqiNP_031557.2. NM_007531.2.
UniGeneiMm.36241.

3D structure databases

ProteinModelPortaliO35129.
SMRiO35129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198310. 12 interactors.
IntActiO35129. 12 interactors.
MINTiMINT-1841450.
STRINGi10090.ENSMUSP00000004375.

PTM databases

iPTMnetiO35129.
PhosphoSitePlusiO35129.
SwissPalmiO35129.

2D gel databases

REPRODUCTION-2DPAGEO35129.

Proteomic databases

EPDiO35129.
PaxDbiO35129.
PeptideAtlasiO35129.
PRIDEiO35129.
TopDownProteomicsiO35129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004375; ENSMUSP00000004375; ENSMUSG00000004264.
GeneIDi12034.
KEGGimmu:12034.
UCSCiuc033iuq.1. mouse.

Organism-specific databases

CTDi11331.
MGIiMGI:102520. Phb2.

Phylogenomic databases

eggNOGiKOG3090. Eukaryota.
COG0330. LUCA.
GeneTreeiENSGT00550000075076.
HOGENOMiHOG000205692.
HOVERGENiHBG004457.
InParanoidiO35129.
KOiK17081.
OMAiSMAERMM.
OrthoDBiEOG091G0IHW.
PhylomeDBiO35129.
TreeFamiTF354230.

Miscellaneous databases

ChiTaRSiPhb2. mouse.
PROiO35129.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004264.
CleanExiMM_PHB2.
ExpressionAtlasiO35129. baseline and differential.
GenevisibleiO35129. MM.

Family and domain databases

CDDicd03401. SPFH_prohibitin. 1 hit.
InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPHB2_MOUSE
AccessioniPrimary (citable) accession number: O35129
Secondary accession number(s): O89075, Q61336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.