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O35129

- PHB2_MOUSE

UniProt

O35129 - PHB2_MOUSE

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Protein

Prohibitin-2

Gene

Phb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.By similarity2 Publications

GO - Biological processi

  1. mammary gland alveolus development Source: MGI
  2. mammary gland branching involved in thelarche Source: MGI
  3. negative regulation of intracellular estrogen receptor signaling pathway Source: MGI
  4. negative regulation of mammary gland epithelial cell proliferation Source: MGI
  5. negative regulation of transcription, DNA-templated Source: UniProtKB
  6. regulation of branching involved in mammary gland duct morphogenesis Source: MGI
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin-2
Alternative name(s):
B-cell receptor-associated protein BAP37
Repressor of estrogen receptor activity
Gene namesi
Name:Phb2By similarity
Synonyms:BapImported, Bcap37Imported, ReaImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:102520. Phb2.

Subcellular locationi

Mitochondrion inner membrane. Cytoplasm. Nucleus
Note: Also cytoplasmic and nuclear.

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. mitochondrial inner membrane Source: UniProtKB
  3. mitochondrion Source: UniProtKB
  4. nuclear matrix Source: UniProtKB
  5. nucleus Source: UniProtKB
  6. protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 299298Prohibitin-2PRO_0000213885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei128 – 1281PhosphotyrosineBy similarity
Modified residuei147 – 1471N6-acetyllysine1 Publication
Modified residuei200 – 2001N6-acetyllysine1 Publication
Modified residuei236 – 2361N6-acetyllysine1 Publication
Modified residuei250 – 2501N6-acetyllysineBy similarity
Modified residuei262 – 2621N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO35129.
PaxDbiO35129.
PRIDEiO35129.

2D gel databases

REPRODUCTION-2DPAGEO35129.

PTM databases

PhosphoSiteiO35129.

Expressioni

Tissue specificityi

Widely expressed in different tissues.3 Publications

Developmental stagei

Throughout gestation, highly expressed in brown fat, heart, liver, developing renal tubules and neurons, and detected at lower levels in tissues such as lung and exocrine pancreas.1 Publication

Gene expression databases

BgeeiO35129.
CleanExiMM_PHB2.
ExpressionAtlasiO35129. baseline and differential.
GenevestigatoriO35129.

Interactioni

Subunit structurei

Interacts with ZNF703 (By similarity). Interacts with STOML2 (By similarity). Interacts with PHB, ESR1, HDAC1 and HDAC5.By similarity3 Publications

Protein-protein interaction databases

BioGridi198310. 11 interactions.
IntActiO35129. 8 interactions.
MINTiMINT-1841450.

Structurei

3D structure databases

ProteinModelPortaliO35129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 4931Necessary for transcriptional repressionBy similarityAdd
BLAST
Regioni150 – 17425Necessary for transcriptional repressionBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili190 – 23849Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the prohibitin family.Sequence Analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0330.
HOGENOMiHOG000205692.
HOVERGENiHBG004457.
InParanoidiO35129.
KOiK17081.
OMAiIGGVKKE.
OrthoDBiEOG7V4B04.
PhylomeDBiO35129.
TreeFamiTF354230.

Family and domain databases

InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35129-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQNLKDLAG RLPAGPRGMG TALKLLLGAG AVAYGVRESV FTVEGGHRAI
60 70 80 90 100
FFNRIGGVQQ DTILAEGLHF RIPWFQYPII YDIRARPRKI SSPTGSKDLQ
110 120 130 140 150
MVNISLRVLS RPNAQELPSM YQRLGLDYEE RVLPSIVNEV LKSVVAKFNA
160 170 180 190 200
SQLITQRAQV SLLIRRELTE RAKDFSLILD DVAITELSFS REYTAAVEAK
210 220 230 240 250
QVAQQEAQRA QFLVEKAKQE QRQKIVQAEG EAEAAKMLGE ALSKNPGYIK
260 270 280 290
LRKIRAAQNI SKTIATSQNR IYLTADNLVL NLQDESFTRG SDSLIKGKK
Length:299
Mass (Da):33,296
Last modified:January 1, 1998 - v1
Checksum:iA887CC982BF85C80
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 682GL → F in CAA55350. (PubMed:8070406)Curated
Sequence conflicti81 – 811Y → N in AAC36528. (PubMed:9798653)Curated
Sequence conflicti112 – 1121P → L in AAC36528. (PubMed:9798653)Curated
Sequence conflicti120 – 1201M → I in AAC36528. (PubMed:9798653)Curated
Sequence conflicti127 – 1271D → G in AAC36528. (PubMed:9798653)Curated
Sequence conflicti138 – 1381N → S in AAC36528. (PubMed:9798653)Curated
Sequence conflicti148 – 1481F → V in AAC36528. (PubMed:9798653)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78683 mRNA. Translation: CAA55350.1.
AY319418 mRNA. Translation: AAP86652.1.
AY211613 mRNA. Translation: AAP47231.1.
AC002397 Genomic DNA. Translation: AAC36005.1.
U89422 mRNA. Translation: AAC36528.1.
CCDSiCCDS39627.1.
PIRiS46996.
RefSeqiNP_031557.2. NM_007531.2.
UniGeneiMm.36241.

Genome annotation databases

EnsembliENSMUST00000004375; ENSMUSP00000004375; ENSMUSG00000004264.
GeneIDi12034.
KEGGimmu:12034.
UCSCiuc009dri.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78683 mRNA. Translation: CAA55350.1 .
AY319418 mRNA. Translation: AAP86652.1 .
AY211613 mRNA. Translation: AAP47231.1 .
AC002397 Genomic DNA. Translation: AAC36005.1 .
U89422 mRNA. Translation: AAC36528.1 .
CCDSi CCDS39627.1.
PIRi S46996.
RefSeqi NP_031557.2. NM_007531.2.
UniGenei Mm.36241.

3D structure databases

ProteinModelPortali O35129.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198310. 11 interactions.
IntActi O35129. 8 interactions.
MINTi MINT-1841450.

PTM databases

PhosphoSitei O35129.

2D gel databases

REPRODUCTION-2DPAGE O35129.

Proteomic databases

MaxQBi O35129.
PaxDbi O35129.
PRIDEi O35129.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000004375 ; ENSMUSP00000004375 ; ENSMUSG00000004264 .
GeneIDi 12034.
KEGGi mmu:12034.
UCSCi uc009dri.1. mouse.

Organism-specific databases

CTDi 11331.
MGIi MGI:102520. Phb2.

Phylogenomic databases

eggNOGi COG0330.
HOGENOMi HOG000205692.
HOVERGENi HBG004457.
InParanoidi O35129.
KOi K17081.
OMAi IGGVKKE.
OrthoDBi EOG7V4B04.
PhylomeDBi O35129.
TreeFami TF354230.

Miscellaneous databases

ChiTaRSi Phb2. mouse.
NextBioi 280279.
PROi O35129.
SOURCEi Search...

Gene expression databases

Bgeei O35129.
CleanExi MM_PHB2.
ExpressionAtlasi O35129. baseline and differential.
Genevestigatori O35129.

Family and domain databases

InterProi IPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view ]
PANTHERi PTHR23222. PTHR23222. 1 hit.
Pfami PF01145. Band_7. 1 hit.
[Graphical view ]
PRINTSi PR00679. PROHIBITIN.
SMARTi SM00244. PHB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein."
    Terashima M., Kim K.-M., Adachi T., Nielsen P.J., Reth M., Koehler G., Lamers M.C.
    EMBO J. 13:3782-3792(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/cImported.
    Tissue: Lymphoid tissue.
  2. "Truncated estrogen receptor product-1 stimulates estrogen receptor alpha transcriptional activity by titration of repressor proteins."
    Lin V.Y., Resnick E.M., Shupnik M.A.
    J. Biol. Chem. 278:38125-38131(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ESR1.
    Strain: 129/SvJImported.
    Tissue: PituitaryImported.
  3. "Transcriptional regulation by the repressor of estrogen receptor activity via recruitment of histone deacetylases."
    Kurtev V., Margueron R., Kroboth K., Ogris E., Cavailles V., Seiser C.
    J. Biol. Chem. 279:24834-24843(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ESR1; HDAC1 AND HDAC5, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: BALB/cImported.
    Tissue: BrainImported.
  4. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
    Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
    Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 25-48; 55-71; 98-142; 148-165; 335-346; 866-885 AND 1051-1058, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
    Chu C.C., Paul W.E.
    Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-154.
    Strain: BALB/cImported.
    Tissue: SpleenImported.
  7. "Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence."
    Coates P.J., Nenutil R., McGregor A., Picksley S.M., Crouch D.H., Hall P.A., Wright E.G.
    Exp. Cell Res. 265:262-273(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PHB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  8. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-147; LYS-200; LYS-236 AND LYS-262, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPHB2_MOUSE
AccessioniPrimary (citable) accession number: O35129
Secondary accession number(s): O89075, Q61336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3