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O35129 (PHB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prohibitin-2
Alternative name(s):
B-cell receptor-associated protein BAP37
Repressor of estrogen receptor activity
Gene names
Name:Phb2
Synonyms:Bap, Bcap37, Rea
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. Ref.2 Ref.3 UniProtKB Q99623

Subunit structure

Interacts with ZNF703 By similarity. Interacts with STOML2 By similarity. Interacts with PHB, ESR1, HDAC1 and HDAC5. Ref.2 Ref.3 Ref.7

Subcellular location

Mitochondrion inner membrane. Cytoplasm. Nucleus. Note: Also cytoplasmic and nuclear. Ref.3 Ref.7

Tissue specificity

Widely expressed in different tissues. Ref.1 Ref.3 Ref.7

Developmental stage

Throughout gestation, highly expressed in brown fat, heart, liver, developing renal tubules and neurons, and detected at lower levels in tissues such as lung and exocrine pancreas. Ref.7

Sequence similarities

Belongs to the prohibitin family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Membrane
Mitochondrion
Mitochondrion inner membrane
Nucleus
   DomainCoiled coil
   Molecular functionReceptor
Repressor
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processmammary gland alveolus development

Inferred from mutant phenotype PubMed 17065319. Source: MGI

mammary gland branching involved in thelarche

Inferred from mutant phenotype PubMed 17065319. Source: MGI

negative regulation of intracellular estrogen receptor signaling pathway

Inferred from mutant phenotype PubMed 17065319. Source: MGI

negative regulation of mammary gland epithelial cell proliferation

Inferred from mutant phenotype PubMed 17065319. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.3. Source: UniProtKB

regulation of branching involved in mammary gland duct morphogenesis

Inferred from mutant phenotype PubMed 17065319. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrial inner membrane

Inferred from direct assay Ref.7. Source: UniProtKB

mitochondrion

Inferred from direct assay PubMed 14651853. Source: UniProtKB

nuclear matrix

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from direct assay Ref.3. Source: UniProtKB

protein complex

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 299298Prohibitin-2
PRO_0000213885

Regions

Region19 – 4931Necessary for transcriptional repression By similarity UniProtKB Q99623
Region150 – 17425Necessary for transcriptional repression By similarity UniProtKB Q99623
Coiled coil190 – 23849 Potential

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1281Phosphotyrosine By similarity
Modified residue1471N6-acetyllysine Ref.8
Modified residue2001N6-acetyllysine Ref.8
Modified residue2361N6-acetyllysine Ref.8
Modified residue2501N6-acetyllysine By similarity
Modified residue2621N6-acetyllysine Ref.8

Experimental info

Sequence conflict67 – 682GL → F in CAA55350. Ref.1
Sequence conflict811Y → N in AAC36528. Ref.6
Sequence conflict1121P → L in AAC36528. Ref.6
Sequence conflict1201M → I in AAC36528. Ref.6
Sequence conflict1271D → G in AAC36528. Ref.6
Sequence conflict1381N → S in AAC36528. Ref.6
Sequence conflict1481F → V in AAC36528. Ref.6

Sequences

Sequence LengthMass (Da)Tools
O35129 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A887CC982BF85C80

FASTA29933,296
        10         20         30         40         50         60 
MAQNLKDLAG RLPAGPRGMG TALKLLLGAG AVAYGVRESV FTVEGGHRAI FFNRIGGVQQ 

        70         80         90        100        110        120 
DTILAEGLHF RIPWFQYPII YDIRARPRKI SSPTGSKDLQ MVNISLRVLS RPNAQELPSM 

       130        140        150        160        170        180 
YQRLGLDYEE RVLPSIVNEV LKSVVAKFNA SQLITQRAQV SLLIRRELTE RAKDFSLILD 

       190        200        210        220        230        240 
DVAITELSFS REYTAAVEAK QVAQQEAQRA QFLVEKAKQE QRQKIVQAEG EAEAAKMLGE 

       250        260        270        280        290 
ALSKNPGYIK LRKIRAAQNI SKTIATSQNR IYLTADNLVL NLQDESFTRG SDSLIKGKK 

« Hide

References

« Hide 'large scale' references
[1]"The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein."
Terashima M., Kim K.-M., Adachi T., Nielsen P.J., Reth M., Koehler G., Lamers M.C.
EMBO J. 13:3782-3792(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Lymphoid tissue.
[2]"Truncated estrogen receptor product-1 stimulates estrogen receptor alpha transcriptional activity by titration of repressor proteins."
Lin V.Y., Resnick E.M., Shupnik M.A.
J. Biol. Chem. 278:38125-38131(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ESR1.
Strain: 129/SvJ.
Tissue: Pituitary.
[3]"Transcriptional regulation by the repressor of estrogen receptor activity via recruitment of histone deacetylases."
Kurtev V., Margueron R., Kroboth K., Ogris E., Cavailles V., Seiser C.
J. Biol. Chem. 279:24834-24843(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ESR1; HDAC1 AND HDAC5, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[4]"Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 25-48; 55-71; 98-142; 148-165; 335-346; 866-885 AND 1051-1058, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[6]"Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
Chu C.C., Paul W.E.
Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-154.
Strain: BALB/c.
Tissue: Spleen.
[7]"Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence."
Coates P.J., Nenutil R., McGregor A., Picksley S.M., Crouch D.H., Hall P.A., Wright E.G.
Exp. Cell Res. 265:262-273(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PHB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-147; LYS-200; LYS-236 AND LYS-262, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78683 mRNA. Translation: CAA55350.1.
AY319418 mRNA. Translation: AAP86652.1.
AY211613 mRNA. Translation: AAP47231.1.
AC002397 Genomic DNA. Translation: AAC36005.1.
U89422 mRNA. Translation: AAC36528.1.
PIRS46996.
RefSeqNP_031557.2. NM_007531.2.
UniGeneMm.36241.

3D structure databases

ProteinModelPortalO35129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198310. 10 interactions.
IntActO35129. 8 interactions.
MINTMINT-1841450.

PTM databases

PhosphoSiteO35129.

2D gel databases

REPRODUCTION-2DPAGEO35129.

Proteomic databases

PaxDbO35129.
PRIDEO35129.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004375; ENSMUSP00000004375; ENSMUSG00000004264.
GeneID12034.
KEGGmmu:12034.
UCSCuc009dri.1. mouse.

Organism-specific databases

CTD11331.
MGIMGI:102520. Phb2.

Phylogenomic databases

eggNOGCOG0330.
HOGENOMHOG000205692.
HOVERGENHBG004457.
InParanoidO35129.
KOK17081.
OMATYMTFSP.
OrthoDBEOG7V4B04.
PhylomeDBO35129.
TreeFamTF354230.

Gene expression databases

ArrayExpressO35129.
BgeeO35129.
CleanExMM_PHB2.
GenevestigatorO35129.

Family and domain databases

InterProIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERPTHR23222. PTHR23222. 1 hit.
PfamPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSPR00679. PROHIBITIN.
SMARTSM00244. PHB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio280279.
PROO35129.
SOURCESearch...

Entry information

Entry namePHB2_MOUSE
AccessionPrimary (citable) accession number: O35129
Secondary accession number(s): O89075, Q61336
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot