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O35119 (TRPC4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Short transient receptor potential channel 4
Short name=Trp4
Short name=TrpC4
Alternative name(s):
Capacitative calcium entry channel 1
Short name=CCE1
Gene names
Name:Trpc4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length977 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thought to form non-selective a receptor-activated calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective By similarity. May also be activated by intracellular calcium store depletion.

Subunit structure

Homotetramer and heterotetramer with TRPC1 and/or TRPC5. Heteromers containing TRPC3 or TRPC6 are also found exclusively in embryonic brain. Isoform alpha but not isoform beta associates with inositol 1,4,5-trisphosphate receptor (ITPR). Interacts with NHERF By similarity. Interacts with MX1 and RNF24 By similarity. Interacts (via CIRB domain) with SESTD1 (via the spectrin 1 repeat) and SPTBN5 (via C-terminus) By similarity. Interacts with CDH5 and CTNNB1 By similarity. Interacts (via protein 4.1-binding domain) with EPB41L2 By similarity. Interacts with TRPC4AP By similarity. Ref.4

Subcellular location

Membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily. [View classification]

Contains 4 ANK repeats.

Sequence caution

The sequence BAA23599.2 differs from that shown. Reason: Frameshift at positions 121 and 412.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: O35119-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: O35119-2)

The sequence of this isoform differs from the canonical sequence as follows:
     784-867: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 977977Short transient receptor potential channel 4
PRO_0000215316

Regions

Topological domain1 – 329329Cytoplasmic Potential
Transmembrane330 – 35021Helical; Potential
Topological domain351 – 36212Extracellular Potential
Transmembrane363 – 38321Helical; Potential
Topological domain384 – 43653Cytoplasmic Potential
Transmembrane437 – 45721Helical; Potential
Topological domain458 – 46912Extracellular Potential
Transmembrane470 – 49021Helical; Potential
Topological domain491 – 51121Cytoplasmic Potential
Transmembrane512 – 53221Helical; Potential
Topological domain533 – 59967Extracellular Potential
Transmembrane600 – 62021Helical; Potential
Topological domain621 – 974354Cytoplasmic Potential
Repeat31 – 6030ANK 1
Repeat69 – 9729ANK 2
Repeat98 – 12427ANK 3
Repeat141 – 17030ANK 4
Region87 – 17286Multimerization domain By similarity
Region254 – 30451Multimerization domain By similarity
Region615 – 977363Binds to ITPR1, ITPR2 and ITPR3 By similarity
Region975 – 9773PDZ-binding domain By similarity
Coiled coil223 – 26038 Potential

Amino acid modifications

Modified residue9591Phosphotyrosine; by FYN By similarity
Modified residue9721Phosphotyrosine; by FYN By similarity

Natural variations

Alternative sequence784 – 86784Missing in isoform Beta.
VSP_006571

Experimental info

Sequence conflict3371Missing in BAA23599. Ref.1
Sequence conflict390 – 41122Missing in BAA23599. Ref.1
Sequence conflict5941D → E in BAA23599. Ref.1
Sequence conflict8881K → Q in BAA23599. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified June 20, 2001. Version 2.
Checksum: 6F86DA95261E0ECD

FASTA977111,848
        10         20         30         40         50         60 
MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK SLEEAEIYFK 

        70         80         90        100        110        120 
ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL LHAIRKEVVG AVELLLNHKK 

       130        140        150        160        170        180 
PSGEKQVPPI LLDKQFSEFT PDITPIILAA HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE 

       190        200        210        220        230        240 
CVSSSDVDSL RHSRSRLNIY KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE 

       250        260        270        280        290        300 
YEELSRQCKQ FAKDLLDQTR SSRELEIILN YRDDNSLIEE QSGNDLARLK LAIKYRQKEF 

       310        320        330        340        350        360 
VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIIGLLF PVFSVCYLIA PKSPLGLFIR 

       370        380        390        400        410        420 
KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT IVEWMILPWV LGFIWGEIKQ 

       430        440        450        460        470        480 
MWDGGLQDYI HDWWNLMDFV MNSLYLATIS LKIVAFVKYS ALNPRESWDM WHPTLVAEAL 

       490        500        510        520        530        540 
FAIANIFSSL RLISLFTANS HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY 

       550        560        570        580        590        600 
YEETKGLSCK GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTDFVGATM 

       610        620        630        640        650        660 
FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG GTLPTPFNVI 

       670        680        690        700        710        720 
PSPKSLWYLV KWIWTHLCKK KMRRKPESFG TIGRRAADNL RRHHQYQEVM RNLVKRYVAA 

       730        740        750        760        770        780 
MIREAKTEEG LTEENVKELK QDISSFRFEV LGLLRGSKLS TIQSANAASS ASSADSDEKS 

       790        800        810        820        830        840 
HSEGNGKDKR KNLSLFDLTT LIHPRSAVIA SERHNLSNGS ALVVQEPPRE KQRKVNFVAD 

       850        860        870        880        890        900 
IKNFGLFHRR SKQNAAEQNA NQIFSVSEEI TRQQAAGALE RNIQLESKGL ASRGDRSIPG 

       910        920        930        940        950        960 
LNEQCVLVDH RERNTDTLGL QVGKRVCSSF KSEKVVVEDT VPIIPKEKHA QEEDSSIDYD 

       970 
LSPTDTVAHE DYVTTRL

« Hide

Isoform Beta [UniParc].

Checksum: A4333318A18017B4
Show »

FASTA893102,425

References

[1]"Cloning and expression localization of cDNA for rat homolog of TRP protein, a possible store-operated calcium (Ca2+) channel."
Funayama M., Goto K., Kondo H.
Brain Res. Mol. Brain Res. 43:259-266(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
Strain: Wistar Imamichi.
Tissue: Brain.
[2]Otsuka Y., Kondo H.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF."
Tang Y., Tang J., Chen Z., Trost C., Flockerzi V., Li M., Ramesh V., Zhu M.X.
J. Biol. Chem. 275:37559-37564(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
Strain: Sprague-Dawley.
[4]"Formation of novel TRPC channels by complex subunit interactions in embryonic brain."
Strubing C., Krapivinsky G., Krapivinsky L., Clapham D.E.
J. Biol. Chem. 278:39014-39019(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB008889 mRNA. Translation: BAA23599.2. Frameshift.
AF288407 mRNA. Translation: AAG21809.1.
AF288408 mRNA. Translation: AAG21810.1.
IPIIPI00231243.
IPI00327223.
UniGeneRn.10853.

3D structure databases

ProteinModelPortalO35119.
ModBaseSearch...

PTM databases

PhosphoSiteO35119.

Proteomic databases

PaxDbO35119.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:621276. rat.

Organism-specific databases

RGD621276. Trpc4.

Phylogenomic databases

eggNOGNOG254238.
HOGENOMHOG000020589.
HOVERGENHBG068337.
InParanoidO35119.

Gene expression databases

ArrayExpressO35119.
GenevestigatorO35119.
GermOnlineENSRNOG00000011133. Rattus norvegicus.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMSSF48403. ANK. 1 hit.
TIGRFAMsTIGR00870. trp. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPC4_RAT
AccessionPrimary (citable) accession number: O35119
Secondary accession number(s): Q9EQ74, Q9EQ75
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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