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O35119

- TRPC4_RAT

UniProt

O35119 - TRPC4_RAT

Protein

Short transient receptor potential channel 4

Gene

Trpc4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (20 Jun 2001)
      Previous versions | rss
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    Functioni

    Thought to form non-selective a receptor-activated calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective By similarity. May also be activated by intracellular calcium store depletion.By similarity

    GO - Molecular functioni

    1. calcium channel activity Source: RGD

    GO - Biological processi

    1. calcium ion transport Source: RGD
    2. regulation of calcium ion transport Source: RGD

    Keywords - Molecular functioni

    Calcium channel, Ion channel

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Short transient receptor potential channel 4
    Short name:
    Trp4
    Short name:
    TrpC4
    Alternative name(s):
    Capacitative calcium entry channel 1
    Short name:
    CCE1
    Gene namesi
    Name:Trpc4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621276. Trpc4.

    Subcellular locationi

    Membrane Curated; Multi-pass membrane protein Curated. Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. integral component of plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 977977Short transient receptor potential channel 4PRO_0000215316Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei959 – 9591Phosphotyrosine; by FYNBy similarity
    Modified residuei972 – 9721Phosphotyrosine; by FYNBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO35119.

    PTM databases

    PhosphoSiteiO35119.

    Expressioni

    Gene expression databases

    GenevestigatoriO35119.

    Interactioni

    Subunit structurei

    Homotetramer and heterotetramer with TRPC1 and/or TRPC5. Heteromers containing TRPC3 or TRPC6 are also found exclusively in embryonic brain. Isoform alpha but not isoform beta associates with inositol 1,4,5-trisphosphate receptor (ITPR). Interacts with NHERF By similarity. Interacts with MX1 and RNF24 By similarity. Interacts (via CIRB domain) with SESTD1 (via the spectrin 1 repeat) and SPTBN5 (via C-terminus) By similarity. Interacts with CDH5 and CTNNB1 By similarity. Interacts (via protein 4.1-binding domain) with EPB41L2 By similarity. Interacts with TRPC4AP By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO35119.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 329329CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini351 – 36212ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini384 – 43653CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini458 – 46912ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini491 – 51121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini533 – 59967ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini621 – 974354CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei330 – 35021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei363 – 38321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei437 – 45721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei470 – 49021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei512 – 53221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei600 – 62021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati31 – 6030ANK 1Add
    BLAST
    Repeati69 – 9729ANK 2Add
    BLAST
    Repeati98 – 12427ANK 3Add
    BLAST
    Repeati141 – 17030ANK 4Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 17286Multimerization domainBy similarityAdd
    BLAST
    Regioni254 – 30451Multimerization domainBy similarityAdd
    BLAST
    Regioni615 – 977363Binds to ITPR1, ITPR2 and ITPR3By similarityAdd
    BLAST
    Regioni975 – 9773PDZ-binding domainBy similarity

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili223 – 26038Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 4 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Coiled coil, Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG254238.
    HOGENOMiHOG000020589.
    HOVERGENiHBG068337.
    InParanoidiO35119.
    PhylomeDBiO35119.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR005821. Ion_trans_dom.
    IPR004729. TRP_channel.
    IPR013555. TRP_dom.
    IPR005460. TRPC4_channel.
    IPR002153. TRPC_channel.
    [Graphical view]
    PANTHERiPTHR10117. PTHR10117. 1 hit.
    PTHR10117:SF25. PTHR10117:SF25. 1 hit.
    PfamiPF00023. Ank. 1 hit.
    PF12796. Ank_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF08344. TRP_2. 1 hit.
    [Graphical view]
    PRINTSiPR01097. TRNSRECEPTRP.
    PR01645. TRPCHANNEL4.
    SMARTiSM00248. ANK. 2 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    TIGRFAMsiTIGR00870. trp. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Alpha (identifier: O35119-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK    50
    SLEEAEIYFK ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL 100
    LHAIRKEVVG AVELLLNHKK PSGEKQVPPI LLDKQFSEFT PDITPIILAA 150
    HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE CVSSSDVDSL RHSRSRLNIY 200
    KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE YEELSRQCKQ 250
    FAKDLLDQTR SSRELEIILN YRDDNSLIEE QSGNDLARLK LAIKYRQKEF 300
    VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIIGLLF PVFSVCYLIA 350
    PKSPLGLFIR KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT 400
    IVEWMILPWV LGFIWGEIKQ MWDGGLQDYI HDWWNLMDFV MNSLYLATIS 450
    LKIVAFVKYS ALNPRESWDM WHPTLVAEAL FAIANIFSSL RLISLFTANS 500
    HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY YEETKGLSCK 550
    GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTDFVGATM 600
    FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG 650
    GTLPTPFNVI PSPKSLWYLV KWIWTHLCKK KMRRKPESFG TIGRRAADNL 700
    RRHHQYQEVM RNLVKRYVAA MIREAKTEEG LTEENVKELK QDISSFRFEV 750
    LGLLRGSKLS TIQSANAASS ASSADSDEKS HSEGNGKDKR KNLSLFDLTT 800
    LIHPRSAVIA SERHNLSNGS ALVVQEPPRE KQRKVNFVAD IKNFGLFHRR 850
    SKQNAAEQNA NQIFSVSEEI TRQQAAGALE RNIQLESKGL ASRGDRSIPG 900
    LNEQCVLVDH RERNTDTLGL QVGKRVCSSF KSEKVVVEDT VPIIPKEKHA 950
    QEEDSSIDYD LSPTDTVAHE DYVTTRL 977
    Length:977
    Mass (Da):111,848
    Last modified:June 20, 2001 - v2
    Checksum:i6F86DA95261E0ECD
    GO
    Isoform Beta (identifier: O35119-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         784-867: Missing.

    Show »
    Length:893
    Mass (Da):102,425
    Checksum:iA4333318A18017B4
    GO

    Sequence cautioni

    The sequence BAA23599.2 differs from that shown. Reason: Frameshift at positions 121 and 412.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti337 – 3371Missing in BAA23599. (PubMed:9037541)Curated
    Sequence conflicti390 – 41122Missing in BAA23599. (PubMed:9037541)CuratedAdd
    BLAST
    Sequence conflicti594 – 5941D → E in BAA23599. (PubMed:9037541)Curated
    Sequence conflicti888 – 8881K → Q in BAA23599. (PubMed:9037541)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei784 – 86784Missing in isoform Beta. 1 PublicationVSP_006571Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008889 mRNA. Translation: BAA23599.2. Frameshift.
    AF288407 mRNA. Translation: AAG21809.1.
    AF288408 mRNA. Translation: AAG21810.1.
    UniGeneiRn.10853.

    Genome annotation databases

    UCSCiRGD:621276. rat. [O35119-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008889 mRNA. Translation: BAA23599.2 . Frameshift.
    AF288407 mRNA. Translation: AAG21809.1 .
    AF288408 mRNA. Translation: AAG21810.1 .
    UniGenei Rn.10853.

    3D structure databases

    ProteinModelPortali O35119.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O35119.

    Proteomic databases

    PaxDbi O35119.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:621276. rat. [O35119-1 ]

    Organism-specific databases

    RGDi 621276. Trpc4.

    Phylogenomic databases

    eggNOGi NOG254238.
    HOGENOMi HOG000020589.
    HOVERGENi HBG068337.
    InParanoidi O35119.
    PhylomeDBi O35119.

    Miscellaneous databases

    PROi O35119.

    Gene expression databases

    Genevestigatori O35119.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR005821. Ion_trans_dom.
    IPR004729. TRP_channel.
    IPR013555. TRP_dom.
    IPR005460. TRPC4_channel.
    IPR002153. TRPC_channel.
    [Graphical view ]
    PANTHERi PTHR10117. PTHR10117. 1 hit.
    PTHR10117:SF25. PTHR10117:SF25. 1 hit.
    Pfami PF00023. Ank. 1 hit.
    PF12796. Ank_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF08344. TRP_2. 1 hit.
    [Graphical view ]
    PRINTSi PR01097. TRNSRECEPTRP.
    PR01645. TRPCHANNEL4.
    SMARTi SM00248. ANK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    TIGRFAMsi TIGR00870. trp. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression localization of cDNA for rat homolog of TRP protein, a possible store-operated calcium (Ca2+) channel."
      Funayama M., Goto K., Kondo H.
      Brain Res. Mol. Brain Res. 43:259-266(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
      Strain: Wistar Imamichi.
      Tissue: Brain.
    2. Otsuka Y., Kondo H.
      Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF."
      Tang Y., Tang J., Chen Z., Trost C., Flockerzi V., Li M., Ramesh V., Zhu M.X.
      J. Biol. Chem. 275:37559-37564(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
      Strain: Sprague-Dawley.
    4. "Formation of novel TRPC channels by complex subunit interactions in embryonic brain."
      Strubing C., Krapivinsky G., Krapivinsky L., Clapham D.E.
      J. Biol. Chem. 278:39014-39019(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiTRPC4_RAT
    AccessioniPrimary (citable) accession number: O35119
    Secondary accession number(s): Q9EQ74, Q9EQ75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: June 20, 2001
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3