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Protein

Short transient receptor potential channel 4

Gene

Trpc4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thought to form non-selective a receptor-activated calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion.By similarity

GO - Molecular functioni

  • calcium channel activity Source: RGD
  • store-operated calcium channel activity Source: GO_Central

GO - Biological processi

  • calcium ion transport Source: RGD
  • manganese ion transport Source: GO_Central
  • regulation of calcium ion transport Source: RGD
  • regulation of cytosolic calcium ion concentration Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Short transient receptor potential channel 4
Short name:
Trp4
Short name:
TrpC4
Alternative name(s):
Capacitative calcium entry channel 1
Short name:
CCE1
Gene namesi
Name:Trpc4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621276. Trpc4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 329CytoplasmicSequence analysisAdd BLAST329
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Topological domaini351 – 362ExtracellularSequence analysisAdd BLAST12
Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
Topological domaini384 – 436CytoplasmicSequence analysisAdd BLAST53
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Topological domaini458 – 469ExtracellularSequence analysisAdd BLAST12
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 511CytoplasmicSequence analysisAdd BLAST21
Transmembranei512 – 532HelicalSequence analysisAdd BLAST21
Topological domaini533 – 599ExtracellularSequence analysisAdd BLAST67
Transmembranei600 – 620HelicalSequence analysisAdd BLAST21
Topological domaini621 – 974CytoplasmicSequence analysisAdd BLAST354

GO - Cellular componenti

  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153161 – 977Short transient receptor potential channel 4Add BLAST977

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei959Phosphotyrosine; by FYNBy similarity1
Modified residuei972Phosphotyrosine; by FYNBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35119.
PRIDEiO35119.

PTM databases

PhosphoSitePlusiO35119.

Interactioni

Subunit structurei

Homotetramer and heterotetramer with TRPC1 and/or TRPC5. Heteromers containing TRPC3 or TRPC6 are also found exclusively in embryonic brain. Isoform alpha but not isoform beta associates with inositol 1,4,5-trisphosphate receptor (ITPR). Interacts with NHERF (By similarity). Interacts with MX1 and RNF24 (By similarity). Interacts (via CIRB domain) with SESTD1 (via the spectrin 1 repeat) and SPTBN5 (via C-terminus) (By similarity). Interacts with CDH5 and CTNNB1 (By similarity). Interacts (via protein 4.1-binding domain) with EPB41L2 (By similarity). Interacts with TRPC4AP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042886.

Structurei

3D structure databases

ProteinModelPortaliO35119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati31 – 60ANK 1Add BLAST30
Repeati69 – 97ANK 2Add BLAST29
Repeati98 – 124ANK 3Add BLAST27
Repeati141 – 170ANK 4Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 172Multimerization domainBy similarityAdd BLAST86
Regioni254 – 304Multimerization domainBy similarityAdd BLAST51
Regioni615 – 977Binds to ITPR1, ITPR2 and ITPR3By similarityAdd BLAST363
Regioni975 – 977PDZ-binding domainBy similarity3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili223 – 260Sequence analysisAdd BLAST38

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
HOGENOMiHOG000020589.
HOVERGENiHBG068337.
InParanoidiO35119.
PhylomeDBiO35119.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamiPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: O35119-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK
60 70 80 90 100
SLEEAEIYFK ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL
110 120 130 140 150
LHAIRKEVVG AVELLLNHKK PSGEKQVPPI LLDKQFSEFT PDITPIILAA
160 170 180 190 200
HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE CVSSSDVDSL RHSRSRLNIY
210 220 230 240 250
KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE YEELSRQCKQ
260 270 280 290 300
FAKDLLDQTR SSRELEIILN YRDDNSLIEE QSGNDLARLK LAIKYRQKEF
310 320 330 340 350
VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIIGLLF PVFSVCYLIA
360 370 380 390 400
PKSPLGLFIR KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT
410 420 430 440 450
IVEWMILPWV LGFIWGEIKQ MWDGGLQDYI HDWWNLMDFV MNSLYLATIS
460 470 480 490 500
LKIVAFVKYS ALNPRESWDM WHPTLVAEAL FAIANIFSSL RLISLFTANS
510 520 530 540 550
HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY YEETKGLSCK
560 570 580 590 600
GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTDFVGATM
610 620 630 640 650
FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG
660 670 680 690 700
GTLPTPFNVI PSPKSLWYLV KWIWTHLCKK KMRRKPESFG TIGRRAADNL
710 720 730 740 750
RRHHQYQEVM RNLVKRYVAA MIREAKTEEG LTEENVKELK QDISSFRFEV
760 770 780 790 800
LGLLRGSKLS TIQSANAASS ASSADSDEKS HSEGNGKDKR KNLSLFDLTT
810 820 830 840 850
LIHPRSAVIA SERHNLSNGS ALVVQEPPRE KQRKVNFVAD IKNFGLFHRR
860 870 880 890 900
SKQNAAEQNA NQIFSVSEEI TRQQAAGALE RNIQLESKGL ASRGDRSIPG
910 920 930 940 950
LNEQCVLVDH RERNTDTLGL QVGKRVCSSF KSEKVVVEDT VPIIPKEKHA
960 970
QEEDSSIDYD LSPTDTVAHE DYVTTRL
Length:977
Mass (Da):111,848
Last modified:June 20, 2001 - v2
Checksum:i6F86DA95261E0ECD
GO
Isoform Beta (identifier: O35119-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     784-867: Missing.

Show »
Length:893
Mass (Da):102,425
Checksum:iA4333318A18017B4
GO

Sequence cautioni

The sequence BAA23599 differs from that shown. Reason: Frameshift at positions 121 and 412.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti337Missing in BAA23599 (PubMed:9037541).Curated1
Sequence conflicti390 – 411Missing in BAA23599 (PubMed:9037541).CuratedAdd BLAST22
Sequence conflicti594D → E in BAA23599 (PubMed:9037541).Curated1
Sequence conflicti888K → Q in BAA23599 (PubMed:9037541).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006571784 – 867Missing in isoform Beta. 1 PublicationAdd BLAST84

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008889 mRNA. Translation: BAA23599.2. Frameshift.
AF288407 mRNA. Translation: AAG21809.1.
AF288408 mRNA. Translation: AAG21810.1.
UniGeneiRn.10853.

Genome annotation databases

UCSCiRGD:621276. rat. [O35119-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008889 mRNA. Translation: BAA23599.2. Frameshift.
AF288407 mRNA. Translation: AAG21809.1.
AF288408 mRNA. Translation: AAG21810.1.
UniGeneiRn.10853.

3D structure databases

ProteinModelPortaliO35119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042886.

PTM databases

PhosphoSitePlusiO35119.

Proteomic databases

PaxDbiO35119.
PRIDEiO35119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621276. rat. [O35119-1]

Organism-specific databases

RGDi621276. Trpc4.

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
HOGENOMiHOG000020589.
HOVERGENiHBG068337.
InParanoidiO35119.
PhylomeDBiO35119.

Miscellaneous databases

PROiO35119.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamiPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPC4_RAT
AccessioniPrimary (citable) accession number: O35119
Secondary accession number(s): Q9EQ74, Q9EQ75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.