Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lysosome membrane protein 2

Gene

Scarb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.2 Publications

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • receptor activity Source: InterPro

GO - Biological processi

  • protein targeting to lysosome Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiR-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome membrane protein 2
Alternative name(s):
85 kDa lysosomal membrane sialoglycoprotein
Short name:
LGP85
Lysosome membrane protein II
Short name:
LIMP II
Scavenger receptor class B member 2
Gene namesi
Name:Scarb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1196458. Scarb2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 4CytoplasmicSequence analysis3
Transmembranei5 – 27HelicalSequence analysisAdd BLAST23
Topological domaini28 – 433LumenalSequence analysisAdd BLAST406
Transmembranei434 – 459HelicalSequence analysisAdd BLAST26
Topological domaini460 – 478CytoplasmicSequence analysisAdd BLAST19

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal lumen Source: BHF-UCL
  • lysosomal membrane Source: BHF-UCL
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi68N → Q: Loss of glycosylation site. Causes retention in the endoplasmic reticulum and abolishes normal location in lysosomes. 1 Publication1
Mutagenesisi105N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi122N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi155L → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi156I → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi159M → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi160L → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi162A → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi163Y → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi166K → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi184I → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi187L → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi191F → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi206N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi224N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi249N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi304N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi325N → Q: Loss of glycosylation site. Causes retention in the endoplasmic reticulum and abolishes normal location in lysosomes. 1 Publication1
Mutagenesisi400D → K: Slightly increased GBA binding. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi412N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi430N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001441562 – 478Lysosome membrane protein 2Add BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi45N-linked (GlcNAc...)Sequence analysis1
Glycosylationi68N-linked (GlcNAc...)Sequence analysis1
Glycosylationi105N-linked (GlcNAc...)1 Publication1
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1
Glycosylationi224N-linked (GlcNAc...)Sequence analysis1
Glycosylationi249N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi274 ↔ 329By similarity
Glycosylationi304N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi312 ↔ 318By similarity
Glycosylationi325N-linked (GlcNAc...)Sequence analysis1
Glycosylationi412N-linked (GlcNAc...)Sequence analysis1
Glycosylationi430N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Acylated by palmitic acid group(s).By similarity
Heavily glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

EPDiO35114.
MaxQBiO35114.
PaxDbiO35114.
PeptideAtlasiO35114.
PRIDEiO35114.

PTM databases

PhosphoSitePlusiO35114.
SwissPalmiO35114.

Expressioni

Tissue specificityi

Detected in the extracts of brain, heart, lung, liver and kidney.

Gene expression databases

BgeeiENSMUSG00000029426.
CleanExiMM_SCARB2.
GenevisibleiO35114. MM.

Interactioni

Subunit structurei

Interacts with GBA.2 Publications

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL

Protein-protein interaction databases

IntActiO35114. 6 interactors.
MINTiMINT-4133971.
STRINGi10090.ENSMUSP00000031377.

Structurei

3D structure databases

ProteinModelPortaliO35114.
SMRiO35114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni155 – 191Important for interaction with GBAAdd BLAST37

Sequence similaritiesi

Belongs to the CD36 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3776. Eukaryota.
ENOG410XS17. LUCA.
GeneTreeiENSGT00530000062927.
HOGENOMiHOG000252951.
HOVERGENiHBG106707.
InParanoidiO35114.
KOiK12384.
OMAiNTICWVA.
OrthoDBiEOG091G0CH9.
PhylomeDBiO35114.
TreeFamiTF317925.

Family and domain databases

InterProiIPR002159. CD36_fam.
IPR005429. LimpII.
[Graphical view]
PANTHERiPTHR11923. PTHR11923. 1 hit.
PfamiPF01130. CD36. 1 hit.
[Graphical view]
PRINTSiPR01609. CD36FAMILY.
PR01611. LIMPII.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35114-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRCCFYTAG TLSLLLLVTS VTLLVARVFQ KAVDQTIEKN MVLQNGTKVF
60 70 80 90 100
NSWEKPPLPV YIQFYFFNVT NPEEILQGEI PLLEEVGPYT YRELRNKANI
110 120 130 140 150
QFGENGTTIS AVTNKAYVFE RNQSVGDPNV DLIRTINIPL LTVVDLAQLT
160 170 180 190 200
LLRELIEAML KAYQQKLFVI HTVHELLWGY KDEILSLVHI FKPDVSPNFG
210 220 230 240 250
LFYERNGTND GEYVFLTGED NYLNFSKIVE WNGKTSLDWW TTDTCNMING
260 270 280 290 300
TDGDSFHPLI SKDEVLYLFP SDLCRSVHIT FSSFENVEGL PAFRYKVPAE
310 320 330 340 350
ILANTSENAG FCIPEGNCMD SGVLNISICK NGAPIIMSFP HFYQADEKFV
360 370 380 390 400
SAIKGMHPNK EEHESFVDIN PLTGIILRGA KRFQINTYVR KLDDFVETGD
410 420 430 440 450
IRTMVFPVMY LNESVLIDKE TANQLKSVIN TTLVVTNIPY IIMALGVFFG
460 470
LVFTWLACRG QGSMDEGTAD ERAPLIRT
Length:478
Mass (Da):54,044
Last modified:January 23, 2007 - v3
Checksum:i55724B77855470DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008553 mRNA. Translation: BAA23372.1.
AK011123 mRNA. Translation: BAB27416.1.
AK083038 mRNA. Translation: BAC38740.1.
AK144235 mRNA. Translation: BAE25789.1.
BC029073 mRNA. Translation: AAH29073.1.
CCDSiCCDS19431.1.
PIRiJC5670.
RefSeqiNP_031670.1. NM_007644.4.
UniGeneiMm.297964.

Genome annotation databases

EnsembliENSMUST00000031377; ENSMUSP00000031377; ENSMUSG00000029426.
GeneIDi12492.
KEGGimmu:12492.
UCSCiuc008ydm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008553 mRNA. Translation: BAA23372.1.
AK011123 mRNA. Translation: BAB27416.1.
AK083038 mRNA. Translation: BAC38740.1.
AK144235 mRNA. Translation: BAE25789.1.
BC029073 mRNA. Translation: AAH29073.1.
CCDSiCCDS19431.1.
PIRiJC5670.
RefSeqiNP_031670.1. NM_007644.4.
UniGeneiMm.297964.

3D structure databases

ProteinModelPortaliO35114.
SMRiO35114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35114. 6 interactors.
MINTiMINT-4133971.
STRINGi10090.ENSMUSP00000031377.

PTM databases

PhosphoSitePlusiO35114.
SwissPalmiO35114.

Proteomic databases

EPDiO35114.
MaxQBiO35114.
PaxDbiO35114.
PeptideAtlasiO35114.
PRIDEiO35114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031377; ENSMUSP00000031377; ENSMUSG00000029426.
GeneIDi12492.
KEGGimmu:12492.
UCSCiuc008ydm.1. mouse.

Organism-specific databases

CTDi950.
MGIiMGI:1196458. Scarb2.

Phylogenomic databases

eggNOGiKOG3776. Eukaryota.
ENOG410XS17. LUCA.
GeneTreeiENSGT00530000062927.
HOGENOMiHOG000252951.
HOVERGENiHBG106707.
InParanoidiO35114.
KOiK12384.
OMAiNTICWVA.
OrthoDBiEOG091G0CH9.
PhylomeDBiO35114.
TreeFamiTF317925.

Enzyme and pathway databases

ReactomeiR-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiO35114.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029426.
CleanExiMM_SCARB2.
GenevisibleiO35114. MM.

Family and domain databases

InterProiIPR002159. CD36_fam.
IPR005429. LimpII.
[Graphical view]
PANTHERiPTHR11923. PTHR11923. 1 hit.
PfamiPF01130. CD36. 1 hit.
[Graphical view]
PRINTSiPR01609. CD36FAMILY.
PR01611. LIMPII.
ProtoNetiSearch...

Entry informationi

Entry nameiSCRB2_MOUSE
AccessioniPrimary (citable) accession number: O35114
Secondary accession number(s): Q3UNF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.