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O35112 (CD166_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
CD166 antigen
Alternative name(s):
Activated leukocyte cell adhesion molecule
HB2
KG-CAM
Protein MEMD
SB-10 antigen
CD_antigen=CD166
Gene names
Name:Alcam
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. May be involved in osteogenesis. May play an important role in the development of a variety of mesenchymal tissues By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Strongest expression in the lung, then brain, liver, and kidney. Present in the somatosensory system, basal ganglia, cortex, olfactory system, and circumventricular organs.

Domain

The CD6 binding site is located in the N-terminal Ig-like domain By similarity.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 583556CD166 antigen
PRO_0000014661

Regions

Topological domain28 – 527500Extracellular Potential
Transmembrane528 – 54922Helical; Potential
Topological domain550 – 58334Cytoplasmic Potential
Domain28 – 12093Ig-like V-type 1
Domain125 – 234110Ig-like V-type 2
Domain245 – 32884Ig-like C2-type 1
Domain333 – 40977Ig-like C2-type 2
Domain416 – 50186Ig-like C2-type 3

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation2651N-linked (GlcNAc...) Potential
Glycosylation3061N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential
Glycosylation4571N-linked (GlcNAc...) Potential
Glycosylation4801N-linked (GlcNAc...) Potential
Glycosylation4991N-linked (GlcNAc...) Potential
Disulfide bond43 ↔ 113 Potential
Disulfide bond157 ↔ 220 Potential
Disulfide bond270 ↔ 313 Potential
Disulfide bond354 ↔ 392 Potential
Disulfide bond435 ↔ 485 Potential

Experimental info

Sequence conflict3391S → G in CAA73693. Ref.5

Sequences

Sequence LengthMass (Da)Tools
O35112 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: D36B73854F5DF61E

FASTA58365,022
        10         20         30         40         50         60 
MASKGSPSCR LVFCLLISAA VLRPGLGWYT VNSAYGDTIV MPCRLDVPQN LMFGKWKYEK 

        70         80         90        100        110        120 
PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI NNAKISDEKR FVCMLVTEDN 

       130        140        150        160        170        180 
VFEAPTLVKV FKQPSKPEIV NRAAFLETEQ LKKLGDCISR DSYPDGNITW YRNGKVLQPV 

       190        200        210        220        230        240 
DGEVSILFKK EIDPGTQLYT MTSSLEYKTT KSDIQMPFTC SVTYYGPSGQ KTIYSEQAIF 

       250        260        270        280        290        300 
DIYYPTEQVT IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQAE GIRSSNTYTL 

       310        320        330        340        350        360 
TDVRRNATGD YKCSLIDQRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL PVSCTISASR 

       370        380        390        400        410        420 
NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM 

       430        440        450        460        470        480 
TKKTDPSGLS KTIICHVEGF PKPAIQWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN 

       490        500        510        520        530        540 
VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADDISDENRE KVNDQAKLIV GIVVGLLLAA 

       550        560        570        580 
LVAGVVYWLY MKKSKTASKH VNKDLGNMEE NKKLEENNHK TEA

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of HB2, a candidate high density lipoprotein receptor. Sequence homology with members of the immunoglobulin superfamily of membrane proteins."
Matsumoto A., Mitchell A., Kurata H., Pyle L., Kondo K., Itakura H., Fidge N.
J. Biol. Chem. 272:16778-16782(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[3]"Distribution and characteristics of a 90 kDa protein, KG-CAM, in the rat CNS."
Peduzzi J.D., Irwin M.H., Geisert E.E. Jr.
Brain Res. 640:296-307(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-38.
[4]Lubec G., Kang S.U., Lubec S.
Submitted (SEP-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 45-55; 58-71; 77-87; 111-129; 143-152; 154-160; 176-189; 306-312 AND 406-423, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[5]"Mesenchymal stem cell surface antigen SB-10 corresponds to activated leukocyte cell adhesion molecule and is involved in osteogenic differentiation."
Bruder S.P., Ricalton N.S., Boynton R.E., Connolly T.J., Jaiswal N., Zaia J., Barry F.P.
J. Bone Miner. Res. 13:655-663(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 94-156 AND 270-361.
Tissue: Mesenchymal cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB008538 mRNA. Translation: BAA23279.1.
BC061970 mRNA. Translation: AAH61970.1.
Y13240 mRNA. Translation: CAA73692.1.
Y13241 mRNA. Translation: CAA73693.1.
IPIIPI00206188.
RefSeqNP_113941.1. NM_031753.1.
UniGeneRn.5789.

3D structure databases

ProteinModelPortalO35112.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000002738.

Proteomic databases

PaxDbO35112.
PRIDEO35112.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000002738; ENSRNOP00000002738; ENSRNOG00000001989.
GeneID79559.
KEGGrno:79559.
UCSCRGD:619972. rat.

Organism-specific databases

CTD214.
RGD619972. Alcam.

Phylogenomic databases

eggNOGNOG148902.
GeneTreeENSGT00530000063457.
HOGENOMHOG000070101.
HOVERGENHBG050847.
InParanoidO35112.
KOK06547.
OrthoDBEOG4933J2.

Gene expression databases

ArrayExpressO35112.
GenevestigatorO35112.

Family and domain databases

Gene3D2.60.40.10. 5 hits.
InterProIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTSM00409. IG. 3 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio614842.

Entry information

Entry nameCD166_RAT
AccessionPrimary (citable) accession number: O35112
Secondary accession number(s): O55172
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 1, 1998
Last modified: April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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