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Protein

CD166 antigen

Gene

Alcam

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. May be involved in osteogenesis. May play an important role in the development of a variety of mesenchymal tissues (By similarity).By similarity

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. motor neuron axon guidance Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_279881. L1CAM interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
CD166 antigen
Alternative name(s):
Activated leukocyte cell adhesion molecule
HB2
KG-CAM
Protein MEMD
SB-10 antigen
CD_antigen: CD166
Gene namesi
Name:Alcam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi619972. Alcam.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 527500ExtracellularSequence AnalysisAdd
BLAST
Transmembranei528 – 54922HelicalSequence AnalysisAdd
BLAST
Topological domaini550 – 58334CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axon Source: Ensembl
  2. external side of plasma membrane Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
  4. focal adhesion Source: Ensembl
  5. integral component of membrane Source: UniProtKB-KW
  6. neuronal cell body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 583556CD166 antigenPRO_0000014661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 113PROSITE-ProRule annotation
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi157 ↔ 220PROSITE-ProRule annotation
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi270 ↔ 313PROSITE-ProRule annotation
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi354 ↔ 392PROSITE-ProRule annotation
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi435 ↔ 485PROSITE-ProRule annotation
Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO35112.
PRIDEiO35112.

Expressioni

Tissue specificityi

Strongest expression in the lung, then brain, liver, and kidney. Present in the somatosensory system, basal ganglia, cortex, olfactory system, and circumventricular organs.

Gene expression databases

GenevestigatoriO35112.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002738.

Structurei

3D structure databases

ProteinModelPortaliO35112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 12093Ig-like V-type 1Add
BLAST
Domaini125 – 234110Ig-like V-type 2Add
BLAST
Domaini245 – 32884Ig-like C2-type 1Add
BLAST
Domaini333 – 40977Ig-like C2-type 2Add
BLAST
Domaini416 – 50186Ig-like C2-type 3Add
BLAST

Domaini

The CD6 binding site is located in the N-terminal Ig-like domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148902.
GeneTreeiENSGT00530000063457.
HOGENOMiHOG000070101.
HOVERGENiHBG050847.
InParanoidiO35112.
KOiK06547.
OMAiIQWTITG.
OrthoDBiEOG74J97B.
PhylomeDBiO35112.
TreeFamiTF321859.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKGSPSCR LVFCLLISAA VLRPGLGWYT VNSAYGDTIV MPCRLDVPQN
60 70 80 90 100
LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI
110 120 130 140 150
NNAKISDEKR FVCMLVTEDN VFEAPTLVKV FKQPSKPEIV NRAAFLETEQ
160 170 180 190 200
LKKLGDCISR DSYPDGNITW YRNGKVLQPV DGEVSILFKK EIDPGTQLYT
210 220 230 240 250
MTSSLEYKTT KSDIQMPFTC SVTYYGPSGQ KTIYSEQAIF DIYYPTEQVT
260 270 280 290 300
IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQAE GIRSSNTYTL
310 320 330 340 350
TDVRRNATGD YKCSLIDQRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL
360 370 380 390 400
PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE
410 420 430 440 450
GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIQWTIT
460 470 480 490 500
GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV
510 520 530 540 550
SAISIPEHDE ADDISDENRE KVNDQAKLIV GIVVGLLLAA LVAGVVYWLY
560 570 580
MKKSKTASKH VNKDLGNMEE NKKLEENNHK TEA
Length:583
Mass (Da):65,022
Last modified:January 1, 1998 - v1
Checksum:iD36B73854F5DF61E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti339 – 3391S → G in CAA73693 (PubMed:9556065).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008538 mRNA. Translation: BAA23279.1.
BC061970 mRNA. Translation: AAH61970.1.
Y13240 mRNA. Translation: CAA73692.1.
Y13241 mRNA. Translation: CAA73693.1.
RefSeqiNP_113941.1. NM_031753.1.
UniGeneiRn.5789.

Genome annotation databases

EnsembliENSRNOT00000002738; ENSRNOP00000002738; ENSRNOG00000001989.
GeneIDi79559.
KEGGirno:79559.
UCSCiRGD:619972. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008538 mRNA. Translation: BAA23279.1.
BC061970 mRNA. Translation: AAH61970.1.
Y13240 mRNA. Translation: CAA73692.1.
Y13241 mRNA. Translation: CAA73693.1.
RefSeqiNP_113941.1. NM_031753.1.
UniGeneiRn.5789.

3D structure databases

ProteinModelPortaliO35112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002738.

Proteomic databases

PaxDbiO35112.
PRIDEiO35112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002738; ENSRNOP00000002738; ENSRNOG00000001989.
GeneIDi79559.
KEGGirno:79559.
UCSCiRGD:619972. rat.

Organism-specific databases

CTDi214.
RGDi619972. Alcam.

Phylogenomic databases

eggNOGiNOG148902.
GeneTreeiENSGT00530000063457.
HOGENOMiHOG000070101.
HOVERGENiHBG050847.
InParanoidiO35112.
KOiK06547.
OMAiIQWTITG.
OrthoDBiEOG74J97B.
PhylomeDBiO35112.
TreeFamiTF321859.

Enzyme and pathway databases

ReactomeiREACT_279881. L1CAM interactions.

Miscellaneous databases

NextBioi614842.
PROiO35112.

Gene expression databases

GenevestigatoriO35112.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of HB2, a candidate high density lipoprotein receptor. Sequence homology with members of the immunoglobulin superfamily of membrane proteins."
    Matsumoto A., Mitchell A., Kurata H., Pyle L., Kondo K., Itakura H., Fidge N.
    J. Biol. Chem. 272:16778-16782(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. "Distribution and characteristics of a 90 kDa protein, KG-CAM, in the rat CNS."
    Peduzzi J.D., Irwin M.H., Geisert E.E. Jr.
    Brain Res. 640:296-307(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-38.
  4. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 45-55; 58-71; 77-87; 111-129; 143-152; 154-160; 176-189; 306-312 AND 406-423, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  5. "Mesenchymal stem cell surface antigen SB-10 corresponds to activated leukocyte cell adhesion molecule and is involved in osteogenic differentiation."
    Bruder S.P., Ricalton N.S., Boynton R.E., Connolly T.J., Jaiswal N., Zaia J., Barry F.P.
    J. Bone Miner. Res. 13:655-663(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 94-156 AND 270-361.
    Tissue: Mesenchymal cell.

Entry informationi

Entry nameiCD166_RAT
AccessioniPrimary (citable) accession number: O35112
Secondary accession number(s): O55172
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 1, 1998
Last modified: April 1, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.