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O35084

- CP27B_MOUSE

UniProt

O35084 - CP27B_MOUSE

Protein

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Gene

Cyp27b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.

    Catalytic activityi

    Calcidiol + NADPH + O2 = calcitriol + NADP+ + H2O.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi454 – 4541Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. calcidiol 1-monooxygenase activity Source: BHF-UCL
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. bone mineralization Source: Ensembl
    2. calcium ion homeostasis Source: Ensembl
    3. calcium ion transport Source: MGI
    4. decidualization Source: Ensembl
    5. G1 to G0 transition Source: Ensembl
    6. negative regulation of calcidiol 1-monooxygenase activity Source: Ensembl
    7. negative regulation of cell growth Source: Ensembl
    8. negative regulation of cell proliferation Source: Ensembl
    9. positive regulation of keratinocyte differentiation Source: Ensembl
    10. positive regulation of vitamin D 24-hydroxylase activity Source: Ensembl
    11. positive regulation of vitamin D receptor signaling pathway Source: Ensembl
    12. regulation of bone mineralization Source: Ensembl
    13. response to estrogen Source: Ensembl
    14. response to interferon-gamma Source: Ensembl
    15. response to lipopolysaccharide Source: Ensembl
    16. response to vitamin D Source: BHF-UCL
    17. vitamin D catabolic process Source: BHF-UCL

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BRENDAi1.14.13.13. 3474.
    ReactomeiREACT_213027. Vitamins.
    REACT_218811. Vitamin D (calciferol) metabolism.
    UniPathwayiUPA00955.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (EC:1.14.13.13)
    Alternative name(s):
    25-OHD-1 alpha-hydroxylase
    25-hydroxyvitamin D(3) 1-alpha-hydroxylase
    Short name:
    VD3 1A hydroxylase
    Calcidiol 1-monooxygenase
    Cytochrome P450 subfamily XXVIIB polypeptide 1
    Cytochrome P450C1 alpha
    Cytochrome P450VD1-alpha
    Cytochrome p450 27B1
    Gene namesi
    Name:Cyp27b1
    Synonyms:Cyp27b, Cyp40
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1098274. Cyp27b1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial membrane Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 50725-hydroxyvitamin D-1 alpha hydroxylase, mitochondrialPRO_0000003623
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Proteomic databases

    PaxDbiO35084.
    PRIDEiO35084.

    PTM databases

    PhosphoSiteiO35084.

    Expressioni

    Tissue specificityi

    Kidney.

    Gene expression databases

    BgeeiO35084.
    GenevestigatoriO35084.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000006907.

    Structurei

    3D structure databases

    ProteinModelPortaliO35084.
    SMRiO35084. Positions 35-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117435.
    HOGENOMiHOG000253961.
    HOVERGENiHBG106909.
    InParanoidiO35084.
    KOiK07438.
    OMAiLHSEITA.
    OrthoDBiEOG7ZGX4B.
    PhylomeDBiO35084.
    TreeFamiTF105094.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O35084-1 [UniParc]FASTAAdd to Basket

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    MTQAVKLASR VFHRIHLPLQ LDASLGSRGS ESVLRSLSDI PGPSTLSFLA    50
    ELFCKGGLSR LHELQVHGAA RYGPIWSGSF GTLRTVYVAD PTLVEQLLRQ 100
    ESHCPERCSF SSWAEHRRRH QRACGLLTAD GEEWQRLRSL LAPLLLRPQA 150
    AAGYAGTLDN VVRDLVRRLR RQRGRGSGLP GLVLDVAGEF YKFGLESIGA 200
    VLLGSRLGCL EAEVPPDTET FIHAVGSVFV STLLTMAMPN WLHHLIPGPW 250
    ARLCRDWDQM FAFAQRHVEL REGEAAMRNQ GKPEEDMPSG HHLTHFLFRE 300
    KVSVQSIVGN VTELLLAGVD TVSNTLSWTL YELSRHPDVQ TALHSEITAG 350
    TRGSCAHPHG TALSQLPLLK AVIKEVLRLY PVVPGNSRVP DRDIRVGNYV 400
    IPQDTLVSLC HYATSRDPTQ FPDPNSFNPA RWLGEGPTPH PFASLPFGFG 450
    KRSCIGRRLA ELELQMALSQ ILTHFEVLPE PGALPIKPMT RTVLVPERSI 500
    NLQFVDR 507
    Length:507
    Mass (Da):56,225
    Last modified:July 15, 1998 - v2
    Checksum:i0669B4478C461B83
    GO

    Sequence cautioni

    The sequence BAA22434.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006034 mRNA. Translation: BAA22434.1. Different initiation.
    AF235021 Genomic DNA. Translation: AAK15024.1.
    AF286219 Genomic DNA. Translation: AAG44889.1.
    CCDSiCCDS24224.2.
    RefSeqiNP_034139.2. NM_010009.2.
    UniGeneiMm.6216.

    Genome annotation databases

    EnsembliENSMUST00000165764; ENSMUSP00000130005; ENSMUSG00000006724.
    GeneIDi13115.
    KEGGimmu:13115.
    UCSCiuc007hht.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006034 mRNA. Translation: BAA22434.1 . Different initiation.
    AF235021 Genomic DNA. Translation: AAK15024.1 .
    AF286219 Genomic DNA. Translation: AAG44889.1 .
    CCDSi CCDS24224.2.
    RefSeqi NP_034139.2. NM_010009.2.
    UniGenei Mm.6216.

    3D structure databases

    ProteinModelPortali O35084.
    SMRi O35084. Positions 35-504.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000006907.

    PTM databases

    PhosphoSitei O35084.

    Proteomic databases

    PaxDbi O35084.
    PRIDEi O35084.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000165764 ; ENSMUSP00000130005 ; ENSMUSG00000006724 .
    GeneIDi 13115.
    KEGGi mmu:13115.
    UCSCi uc007hht.2. mouse.

    Organism-specific databases

    CTDi 1594.
    MGIi MGI:1098274. Cyp27b1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117435.
    HOGENOMi HOG000253961.
    HOVERGENi HBG106909.
    InParanoidi O35084.
    KOi K07438.
    OMAi LHSEITA.
    OrthoDBi EOG7ZGX4B.
    PhylomeDBi O35084.
    TreeFami TF105094.

    Enzyme and pathway databases

    UniPathwayi UPA00955 .
    BRENDAi 1.14.13.13. 3474.
    Reactomei REACT_213027. Vitamins.
    REACT_218811. Vitamin D (calciferol) metabolism.

    Miscellaneous databases

    NextBioi 283134.
    PROi O35084.
    SOURCEi Search...

    Gene expression databases

    Bgeei O35084.
    Genevestigatori O35084.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "25-hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis."
      Takeyama K., Kitanaka S., Sato T., Kobori M., Yanagisawa J., Kato S.
      Science 277:1827-1830(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. "Cloning of the mouse 25-hydroxyvitamin D3 1-alpha hydroxylase gene."
      Kimmel-Jehan C., DeLuca H.F.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 129.
    3. "25-hydroxyvitamin D 1alpha-hydroxylase: structure of the mouse gene, chromosomal assignment, and developmental expression."
      Panda D.K., Al-Kawas S., Seldin M.F., Hendy G.N., Goltzman D.
      J. Bone Miner. Res. 16:46-56(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.

    Entry informationi

    Entry nameiCP27B_MOUSE
    AccessioniPrimary (citable) accession number: O35084
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3