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Protein

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Gene

Cyp27b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.

Catalytic activityi

Calcidiol + NADPH + O2 = calcitriol + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathway: cholecalciferol biosynthesis

This protein is involved in the pathway cholecalciferol biosynthesis, which is part of Hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway cholecalciferol biosynthesis and in Hormone biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi454 – 4541Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • calcidiol 1-monooxygenase activity Source: BHF-UCL
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.13. 3474.
ReactomeiREACT_315248. Vitamins.
REACT_352248. Vitamin D (calciferol) metabolism.
UniPathwayiUPA00955.

Names & Taxonomyi

Protein namesi
Recommended name:
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (EC:1.14.13.13)
Alternative name(s):
25-OHD-1 alpha-hydroxylase
25-hydroxyvitamin D(3) 1-alpha-hydroxylase
Short name:
VD3 1A hydroxylase
Calcidiol 1-monooxygenase
Cytochrome P450 subfamily XXVIIB polypeptide 1
Cytochrome P450C1 alpha
Cytochrome P450VD1-alpha
Cytochrome p450 27B1
Gene namesi
Name:Cyp27b1
Synonyms:Cyp27b, Cyp40
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1098274. Cyp27b1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 50725-hydroxyvitamin D-1 alpha hydroxylase, mitochondrialPRO_0000003623
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

PaxDbiO35084.
PRIDEiO35084.

PTM databases

PhosphoSiteiO35084.

Expressioni

Tissue specificityi

Kidney.

Gene expression databases

BgeeiO35084.
ExpressionAtlasiO35084. baseline and differential.
GenevisibleiO35084. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130005.

Structurei

3D structure databases

ProteinModelPortaliO35084.
SMRiO35084. Positions 35-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000119243.
HOGENOMiHOG000253961.
HOVERGENiHBG106909.
InParanoidiO35084.
KOiK07438.
OMAiDIHVGDY.
OrthoDBiEOG7ZGX4B.
PhylomeDBiO35084.
TreeFamiTF105094.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQAVKLASR VFHRIHLPLQ LDASLGSRGS ESVLRSLSDI PGPSTLSFLA
60 70 80 90 100
ELFCKGGLSR LHELQVHGAA RYGPIWSGSF GTLRTVYVAD PTLVEQLLRQ
110 120 130 140 150
ESHCPERCSF SSWAEHRRRH QRACGLLTAD GEEWQRLRSL LAPLLLRPQA
160 170 180 190 200
AAGYAGTLDN VVRDLVRRLR RQRGRGSGLP GLVLDVAGEF YKFGLESIGA
210 220 230 240 250
VLLGSRLGCL EAEVPPDTET FIHAVGSVFV STLLTMAMPN WLHHLIPGPW
260 270 280 290 300
ARLCRDWDQM FAFAQRHVEL REGEAAMRNQ GKPEEDMPSG HHLTHFLFRE
310 320 330 340 350
KVSVQSIVGN VTELLLAGVD TVSNTLSWTL YELSRHPDVQ TALHSEITAG
360 370 380 390 400
TRGSCAHPHG TALSQLPLLK AVIKEVLRLY PVVPGNSRVP DRDIRVGNYV
410 420 430 440 450
IPQDTLVSLC HYATSRDPTQ FPDPNSFNPA RWLGEGPTPH PFASLPFGFG
460 470 480 490 500
KRSCIGRRLA ELELQMALSQ ILTHFEVLPE PGALPIKPMT RTVLVPERSI

NLQFVDR
Length:507
Mass (Da):56,225
Last modified:July 15, 1998 - v2
Checksum:i0669B4478C461B83
GO

Sequence cautioni

The sequence BAA22434.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006034 mRNA. Translation: BAA22434.1. Different initiation.
AF235021 Genomic DNA. Translation: AAK15024.1.
AF286219 Genomic DNA. Translation: AAG44889.1.
CCDSiCCDS24224.2.
RefSeqiNP_034139.2. NM_010009.2.
UniGeneiMm.6216.

Genome annotation databases

EnsembliENSMUST00000165764; ENSMUSP00000130005; ENSMUSG00000006724.
GeneIDi13115.
KEGGimmu:13115.
UCSCiuc007hht.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006034 mRNA. Translation: BAA22434.1. Different initiation.
AF235021 Genomic DNA. Translation: AAK15024.1.
AF286219 Genomic DNA. Translation: AAG44889.1.
CCDSiCCDS24224.2.
RefSeqiNP_034139.2. NM_010009.2.
UniGeneiMm.6216.

3D structure databases

ProteinModelPortaliO35084.
SMRiO35084. Positions 35-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130005.

PTM databases

PhosphoSiteiO35084.

Proteomic databases

PaxDbiO35084.
PRIDEiO35084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000165764; ENSMUSP00000130005; ENSMUSG00000006724.
GeneIDi13115.
KEGGimmu:13115.
UCSCiuc007hht.2. mouse.

Organism-specific databases

CTDi1594.
MGIiMGI:1098274. Cyp27b1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000119243.
HOGENOMiHOG000253961.
HOVERGENiHBG106909.
InParanoidiO35084.
KOiK07438.
OMAiDIHVGDY.
OrthoDBiEOG7ZGX4B.
PhylomeDBiO35084.
TreeFamiTF105094.

Enzyme and pathway databases

UniPathwayiUPA00955.
BRENDAi1.14.13.13. 3474.
ReactomeiREACT_315248. Vitamins.
REACT_352248. Vitamin D (calciferol) metabolism.

Miscellaneous databases

NextBioi283134.
PROiO35084.
SOURCEiSearch...

Gene expression databases

BgeeiO35084.
ExpressionAtlasiO35084. baseline and differential.
GenevisibleiO35084. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "25-hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis."
    Takeyama K., Kitanaka S., Sato T., Kobori M., Yanagisawa J., Kato S.
    Science 277:1827-1830(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Cloning of the mouse 25-hydroxyvitamin D3 1-alpha hydroxylase gene."
    Kimmel-Jehan C., DeLuca H.F.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129.
  3. "25-hydroxyvitamin D 1alpha-hydroxylase: structure of the mouse gene, chromosomal assignment, and developmental expression."
    Panda D.K., Al-Kawas S., Seldin M.F., Hendy G.N., Goltzman D.
    J. Bone Miner. Res. 16:46-56(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.

Entry informationi

Entry nameiCP27B_MOUSE
AccessioniPrimary (citable) accession number: O35084
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: June 24, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.