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Protein

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Gene

Cyp27b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)2D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)3D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.1 Publication

Catalytic activityi

Calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O.1 Publication
Secalciferol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O.1 Publication

Cofactori

hemeBy similarity

Kineticsi

  1. KM=2.7 µM for 25-hydroxyvitamin D31 Publication
  2. KM=1.3 µM for 24,25-dihydroxyvitamin D31 Publication
  1. Vmax=9.5 pmol/min/mg enzyme with 25-hydroxyvitamin D3 as substrate1 Publication
  2. Vmax=16.7 pmol/min/mg enzyme with 24,25-dihydroxyvitamin D3 as substrate1 Publication

Pathwayi: cholecalciferol biosynthesis

This protein is involved in the pathway cholecalciferol biosynthesis, which is part of Hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway cholecalciferol biosynthesis and in Hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi454Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • calcidiol 1-monooxygenase activity Source: BHF-UCL
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.13.13 3474
ReactomeiR-MMU-196791 Vitamin D (calciferol) metabolism
R-MMU-211916 Vitamins
UniPathwayiUPA00955

Chemistry databases

SwissLipidsiSLP:000001485

Names & Taxonomyi

Protein namesi
Recommended name:
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (EC:1.14.15.181 Publication)
Alternative name(s):
25-OHD-1 alpha-hydroxylase
25-hydroxyvitamin D(3) 1-alpha-hydroxylase
Short name:
VD3 1A hydroxylase
Calcidiol 1-monooxygenase
Cytochrome P450 subfamily XXVIIB polypeptide 1
Cytochrome P450C1 alpha
Cytochrome P450VD1-alpha
Cytochrome p450 27B1
Gene namesi
Name:Cyp27b1
Synonyms:Cyp27b, Cyp40
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1098274 Cyp27b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3329080

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000003623? – 50725-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiO35084
PRIDEiO35084

PTM databases

iPTMnetiO35084
PhosphoSitePlusiO35084

Expressioni

Tissue specificityi

Kidney.

Gene expression databases

BgeeiENSMUSG00000006724
ExpressionAtlasiO35084 baseline and differential
GenevisibleiO35084 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130005

Chemistry databases

BindingDBiO35084

Structurei

3D structure databases

ProteinModelPortaliO35084
SMRiO35084
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0159 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140779
HOGENOMiHOG000253961
HOVERGENiHBG106909
InParanoidiO35084
KOiK07438
OMAiNSWRFAP
OrthoDBiEOG091G0MI3
PhylomeDBiO35084
TreeFamiTF105094

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQAVKLASR VFHRIHLPLQ LDASLGSRGS ESVLRSLSDI PGPSTLSFLA
60 70 80 90 100
ELFCKGGLSR LHELQVHGAA RYGPIWSGSF GTLRTVYVAD PTLVEQLLRQ
110 120 130 140 150
ESHCPERCSF SSWAEHRRRH QRACGLLTAD GEEWQRLRSL LAPLLLRPQA
160 170 180 190 200
AAGYAGTLDN VVRDLVRRLR RQRGRGSGLP GLVLDVAGEF YKFGLESIGA
210 220 230 240 250
VLLGSRLGCL EAEVPPDTET FIHAVGSVFV STLLTMAMPN WLHHLIPGPW
260 270 280 290 300
ARLCRDWDQM FAFAQRHVEL REGEAAMRNQ GKPEEDMPSG HHLTHFLFRE
310 320 330 340 350
KVSVQSIVGN VTELLLAGVD TVSNTLSWTL YELSRHPDVQ TALHSEITAG
360 370 380 390 400
TRGSCAHPHG TALSQLPLLK AVIKEVLRLY PVVPGNSRVP DRDIRVGNYV
410 420 430 440 450
IPQDTLVSLC HYATSRDPTQ FPDPNSFNPA RWLGEGPTPH PFASLPFGFG
460 470 480 490 500
KRSCIGRRLA ELELQMALSQ ILTHFEVLPE PGALPIKPMT RTVLVPERSI

NLQFVDR
Length:507
Mass (Da):56,225
Last modified:July 15, 1998 - v2
Checksum:i0669B4478C461B83
GO

Sequence cautioni

The sequence BAA22434 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006034 mRNA Translation: BAA22434.1 Different initiation.
AF235021 Genomic DNA Translation: AAK15024.1
AF286219 Genomic DNA Translation: AAG44889.1
CCDSiCCDS24224.2
RefSeqiNP_034139.2, NM_010009.2
UniGeneiMm.6216

Genome annotation databases

EnsembliENSMUST00000165764; ENSMUSP00000130005; ENSMUSG00000006724
GeneIDi13115
KEGGimmu:13115
UCSCiuc007hhs.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCP27B_MOUSE
AccessioniPrimary (citable) accession number: O35084
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: May 23, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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