Reviewed, UniProtKB/Swiss-Prot O35084 (CP27B_MOUSE)

Names and origin

Protein names

Recommended name:
    25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    EC=1.14.13.13
Alternative name(s):
    Cytochrome P450 subfamily XXVIIB polypeptide 1
    Cytochrome p450 27B1
    Calcidiol 1-monooxygenase
    25-OHD-1 alpha-hydroxylase
    25-hydroxyvitamin D(3) 1-alpha-hydroxylase
      Short name=VD3 1A hydroxylase
    P450C1 alpha
    P450VD1-alpha

Gene names

Name:	Cyp27b1
Synonyms:	Cyp27b, Cyp40

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Catalytic activity	Calcidiol + NADPH + O2 = calcitriol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Hormone biosynthesis; cholecalciferol biosynthesis.
Subcellular location	Mitochondrion membrane.
Tissue specificity	Kidney.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	calcium ion transport Inferred from mutant phenotype. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to vitamin D Ref.1 Inferred from direct assay. Source: UniProtKB vitamin D catabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcidiol 1-monooxygenase activity Ref.1 Inferred from direct assay. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 507		25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial		PRO_0000003623
Sites
	Metal binding	454	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35084-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 0669B4478C461B83	FASTA	507	56,225
	10 20 30 40 50 60 MTQAVKLASR VFHRIHLPLQ LDASLGSRGS ESVLRSLSDI PGPSTLSFLA ELFCKGGLSR 70 80 90 100 110 120 LHELQVHGAA RYGPIWSGSF GTLRTVYVAD PTLVEQLLRQ ESHCPERCSF SSWAEHRRRH 130 140 150 160 170 180 QRACGLLTAD GEEWQRLRSL LAPLLLRPQA AAGYAGTLDN VVRDLVRRLR RQRGRGSGLP 190 200 210 220 230 240 GLVLDVAGEF YKFGLESIGA VLLGSRLGCL EAEVPPDTET FIHAVGSVFV STLLTMAMPN 250 260 270 280 290 300 WLHHLIPGPW ARLCRDWDQM FAFAQRHVEL REGEAAMRNQ GKPEEDMPSG HHLTHFLFRE 310 320 330 340 350 360 KVSVQSIVGN VTELLLAGVD TVSNTLSWTL YELSRHPDVQ TALHSEITAG TRGSCAHPHG 370 380 390 400 410 420 TALSQLPLLK AVIKEVLRLY PVVPGNSRVP DRDIRVGNYV IPQDTLVSLC HYATSRDPTQ 430 440 450 460 470 480 FPDPNSFNPA RWLGEGPTPH PFASLPFGFG KRSCIGRRLA ELELQMALSQ ILTHFEVLPE 490 500 PGALPIKPMT RTVLVPERSI NLQFVDR

References

[1]	"25-hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis." Takeyama K., Kitanaka S., Sato T., Kobori M., Yanagisawa J., Kato S. Science 277:1827-1830(1997) [PubMed: 9295274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Cloning of the mouse 25-hydroxyvitamin D3 1-alpha hydroxylase gene." Kimmel-Jehan C., DeLuca H.F. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 129.
[3]	"25-hydroxyvitamin D 1alpha-hydroxylase: structure of the mouse gene, chromosomal assignment, and developmental expression." Panda D.K., Al-Kawas S., Seldin M.F., Hendy G.N., Goltzman D. J. Bone Miner. Res. 16:46-56(2001) [PubMed: 11149489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB006034 mRNA. Translation: BAA22434.1. Different initiation. AF235021 Genomic DNA. Translation: AAK15024.1. AF286219 Genomic DNA. Translation: AAG44889.1.
IPI	IPI00126549.
UniGene	Mm.6216
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000006724. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1098274. Cyp27b1.
Phylogenomic databases
HOGENOM	O35084.
HOVERGEN	O35084.
Enzyme and pathway databases
BRENDA	1.14.13.13. 244.
Gene expression databases
ArrayExpress	O35084.
Bgee	O35084.
GermOnline	ENSMUSG00000006724. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CP27B_MOUSE

Accession

Primary (citable) accession number: O35084

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)