Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O35084

- CP27B_MOUSE

UniProt

O35084 - CP27B_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Gene

Cyp27b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.

Catalytic activityi

Calcidiol + NADPH + O2 = calcitriol + NADP+ + H2O.

Cofactori

Heme group.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi454 – 4541Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. calcidiol 1-monooxygenase activity Source: BHF-UCL
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. bone mineralization Source: Ensembl
  2. calcitriol biosynthetic process from calciol Source: GOC
  3. calcium ion homeostasis Source: Ensembl
  4. calcium ion transport Source: MGI
  5. decidualization Source: Ensembl
  6. G1 to G0 transition Source: Ensembl
  7. negative regulation of calcidiol 1-monooxygenase activity Source: Ensembl
  8. negative regulation of cell growth Source: Ensembl
  9. negative regulation of cell proliferation Source: Ensembl
  10. positive regulation of keratinocyte differentiation Source: Ensembl
  11. positive regulation of vitamin D 24-hydroxylase activity Source: Ensembl
  12. positive regulation of vitamin D receptor signaling pathway Source: Ensembl
  13. regulation of bone mineralization Source: Ensembl
  14. response to estrogen Source: Ensembl
  15. response to interferon-gamma Source: Ensembl
  16. response to lipopolysaccharide Source: Ensembl
  17. response to vitamin D Source: BHF-UCL
  18. vitamin D catabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.13. 3474.
ReactomeiREACT_213027. Vitamins.
REACT_218811. Vitamin D (calciferol) metabolism.
UniPathwayiUPA00955.

Names & Taxonomyi

Protein namesi
Recommended name:
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (EC:1.14.13.13)
Alternative name(s):
25-OHD-1 alpha-hydroxylase
25-hydroxyvitamin D(3) 1-alpha-hydroxylase
Short name:
VD3 1A hydroxylase
Calcidiol 1-monooxygenase
Cytochrome P450 subfamily XXVIIB polypeptide 1
Cytochrome P450C1 alpha
Cytochrome P450VD1-alpha
Cytochrome p450 27B1
Gene namesi
Name:Cyp27b1
Synonyms:Cyp27b, Cyp40
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1098274. Cyp27b1.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB-KW
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 50725-hydroxyvitamin D-1 alpha hydroxylase, mitochondrialPRO_0000003623
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

PaxDbiO35084.
PRIDEiO35084.

PTM databases

PhosphoSiteiO35084.

Expressioni

Tissue specificityi

Kidney.

Gene expression databases

BgeeiO35084.
ExpressionAtlasiO35084. baseline and differential.
GenevestigatoriO35084.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006907.

Structurei

3D structure databases

ProteinModelPortaliO35084.
SMRiO35084. Positions 35-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000119243.
HOGENOMiHOG000253961.
HOVERGENiHBG106909.
InParanoidiO35084.
KOiK07438.
OMAiLHSEITA.
OrthoDBiEOG7ZGX4B.
PhylomeDBiO35084.
TreeFamiTF105094.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35084-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQAVKLASR VFHRIHLPLQ LDASLGSRGS ESVLRSLSDI PGPSTLSFLA
60 70 80 90 100
ELFCKGGLSR LHELQVHGAA RYGPIWSGSF GTLRTVYVAD PTLVEQLLRQ
110 120 130 140 150
ESHCPERCSF SSWAEHRRRH QRACGLLTAD GEEWQRLRSL LAPLLLRPQA
160 170 180 190 200
AAGYAGTLDN VVRDLVRRLR RQRGRGSGLP GLVLDVAGEF YKFGLESIGA
210 220 230 240 250
VLLGSRLGCL EAEVPPDTET FIHAVGSVFV STLLTMAMPN WLHHLIPGPW
260 270 280 290 300
ARLCRDWDQM FAFAQRHVEL REGEAAMRNQ GKPEEDMPSG HHLTHFLFRE
310 320 330 340 350
KVSVQSIVGN VTELLLAGVD TVSNTLSWTL YELSRHPDVQ TALHSEITAG
360 370 380 390 400
TRGSCAHPHG TALSQLPLLK AVIKEVLRLY PVVPGNSRVP DRDIRVGNYV
410 420 430 440 450
IPQDTLVSLC HYATSRDPTQ FPDPNSFNPA RWLGEGPTPH PFASLPFGFG
460 470 480 490 500
KRSCIGRRLA ELELQMALSQ ILTHFEVLPE PGALPIKPMT RTVLVPERSI

NLQFVDR
Length:507
Mass (Da):56,225
Last modified:July 15, 1998 - v2
Checksum:i0669B4478C461B83
GO

Sequence cautioni

The sequence BAA22434.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB006034 mRNA. Translation: BAA22434.1. Different initiation.
AF235021 Genomic DNA. Translation: AAK15024.1.
AF286219 Genomic DNA. Translation: AAG44889.1.
CCDSiCCDS24224.2.
RefSeqiNP_034139.2. NM_010009.2.
UniGeneiMm.6216.

Genome annotation databases

EnsembliENSMUST00000165764; ENSMUSP00000130005; ENSMUSG00000006724.
GeneIDi13115.
KEGGimmu:13115.
UCSCiuc007hht.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB006034 mRNA. Translation: BAA22434.1 . Different initiation.
AF235021 Genomic DNA. Translation: AAK15024.1 .
AF286219 Genomic DNA. Translation: AAG44889.1 .
CCDSi CCDS24224.2.
RefSeqi NP_034139.2. NM_010009.2.
UniGenei Mm.6216.

3D structure databases

ProteinModelPortali O35084.
SMRi O35084. Positions 35-504.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000006907.

PTM databases

PhosphoSitei O35084.

Proteomic databases

PaxDbi O35084.
PRIDEi O35084.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000165764 ; ENSMUSP00000130005 ; ENSMUSG00000006724 .
GeneIDi 13115.
KEGGi mmu:13115.
UCSCi uc007hht.2. mouse.

Organism-specific databases

CTDi 1594.
MGIi MGI:1098274. Cyp27b1.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000119243.
HOGENOMi HOG000253961.
HOVERGENi HBG106909.
InParanoidi O35084.
KOi K07438.
OMAi LHSEITA.
OrthoDBi EOG7ZGX4B.
PhylomeDBi O35084.
TreeFami TF105094.

Enzyme and pathway databases

UniPathwayi UPA00955 .
BRENDAi 1.14.13.13. 3474.
Reactomei REACT_213027. Vitamins.
REACT_218811. Vitamin D (calciferol) metabolism.

Miscellaneous databases

NextBioi 283134.
PROi O35084.
SOURCEi Search...

Gene expression databases

Bgeei O35084.
ExpressionAtlasi O35084. baseline and differential.
Genevestigatori O35084.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "25-hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis."
    Takeyama K., Kitanaka S., Sato T., Kobori M., Yanagisawa J., Kato S.
    Science 277:1827-1830(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Cloning of the mouse 25-hydroxyvitamin D3 1-alpha hydroxylase gene."
    Kimmel-Jehan C., DeLuca H.F.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129.
  3. "25-hydroxyvitamin D 1alpha-hydroxylase: structure of the mouse gene, chromosomal assignment, and developmental expression."
    Panda D.K., Al-Kawas S., Seldin M.F., Hendy G.N., Goltzman D.
    J. Bone Miner. Res. 16:46-56(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.

Entry informationi

Entry nameiCP27B_MOUSE
AccessioniPrimary (citable) accession number: O35084
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3