Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Gene

Cyp27b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)2D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)3D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.1 Publication

Catalytic activityi

Calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O.1 Publication
Secalciferol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O.1 Publication

Cofactori

hemeBy similarity

Kineticsi

  1. KM=2.7 µM for 25-hydroxyvitamin D31 Publication
  2. KM=1.3 µM for 24,25-dihydroxyvitamin D31 Publication
  1. Vmax=9.5 pmol/min/mg enzyme with 25-hydroxyvitamin D3 as substrate1 Publication
  2. Vmax=16.7 pmol/min/mg enzyme with 24,25-dihydroxyvitamin D3 as substrate1 Publication

Pathwayi: cholecalciferol biosynthesis

This protein is involved in the pathway cholecalciferol biosynthesis, which is part of Hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway cholecalciferol biosynthesis and in Hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi454Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • calcidiol 1-monooxygenase activity Source: BHF-UCL
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.13. 3474.
ReactomeiR-MMU-196791. Vitamin D (calciferol) metabolism.
R-MMU-211916. Vitamins.
UniPathwayiUPA00955.

Chemistry databases

SwissLipidsiSLP:000001485.

Names & Taxonomyi

Protein namesi
Recommended name:
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (EC:1.14.15.181 Publication)
Alternative name(s):
25-OHD-1 alpha-hydroxylase
25-hydroxyvitamin D(3) 1-alpha-hydroxylase
Short name:
VD3 1A hydroxylase
Calcidiol 1-monooxygenase
Cytochrome P450 subfamily XXVIIB polypeptide 1
Cytochrome P450C1 alpha
Cytochrome P450VD1-alpha
Cytochrome p450 27B1
Gene namesi
Name:Cyp27b1
Synonyms:Cyp27b, Cyp40
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1098274. Cyp27b1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3329080.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000003623? – 50725-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiO35084.
PRIDEiO35084.

PTM databases

iPTMnetiO35084.
PhosphoSitePlusiO35084.

Expressioni

Tissue specificityi

Kidney.

Gene expression databases

BgeeiENSMUSG00000006724.
ExpressionAtlasiO35084. baseline and differential.
GenevisibleiO35084. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130005.

Chemistry databases

BindingDBiO35084.

Structurei

3D structure databases

ProteinModelPortaliO35084.
SMRiO35084.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0159. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00900000140779.
HOGENOMiHOG000253961.
HOVERGENiHBG106909.
InParanoidiO35084.
KOiK07438.
OMAiNSWRFAP.
OrthoDBiEOG091G0MI3.
PhylomeDBiO35084.
TreeFamiTF105094.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR036396. Cyt_P450_sf.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQAVKLASR VFHRIHLPLQ LDASLGSRGS ESVLRSLSDI PGPSTLSFLA
60 70 80 90 100
ELFCKGGLSR LHELQVHGAA RYGPIWSGSF GTLRTVYVAD PTLVEQLLRQ
110 120 130 140 150
ESHCPERCSF SSWAEHRRRH QRACGLLTAD GEEWQRLRSL LAPLLLRPQA
160 170 180 190 200
AAGYAGTLDN VVRDLVRRLR RQRGRGSGLP GLVLDVAGEF YKFGLESIGA
210 220 230 240 250
VLLGSRLGCL EAEVPPDTET FIHAVGSVFV STLLTMAMPN WLHHLIPGPW
260 270 280 290 300
ARLCRDWDQM FAFAQRHVEL REGEAAMRNQ GKPEEDMPSG HHLTHFLFRE
310 320 330 340 350
KVSVQSIVGN VTELLLAGVD TVSNTLSWTL YELSRHPDVQ TALHSEITAG
360 370 380 390 400
TRGSCAHPHG TALSQLPLLK AVIKEVLRLY PVVPGNSRVP DRDIRVGNYV
410 420 430 440 450
IPQDTLVSLC HYATSRDPTQ FPDPNSFNPA RWLGEGPTPH PFASLPFGFG
460 470 480 490 500
KRSCIGRRLA ELELQMALSQ ILTHFEVLPE PGALPIKPMT RTVLVPERSI

NLQFVDR
Length:507
Mass (Da):56,225
Last modified:July 15, 1998 - v2
Checksum:i0669B4478C461B83
GO

Sequence cautioni

O35084: The sequence BAA22434 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006034 mRNA. Translation: BAA22434.1. Different initiation.
AF235021 Genomic DNA. Translation: AAK15024.1.
AF286219 Genomic DNA. Translation: AAG44889.1.
CCDSiCCDS24224.2.
RefSeqiNP_034139.2. NM_010009.2.
UniGeneiMm.6216.

Genome annotation databases

EnsembliENSMUST00000165764; ENSMUSP00000130005; ENSMUSG00000006724.
GeneIDi13115.
KEGGimmu:13115.
UCSCiuc007hhs.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiCP27B_MOUSE
AccessioniPrimary (citable) accession number: O35084
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: October 25, 2017
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families