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O35084 (CP27B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

EC=1.14.13.13
Alternative name(s):
25-OHD-1 alpha-hydroxylase
25-hydroxyvitamin D(3) 1-alpha-hydroxylase
Short name=VD3 1A hydroxylase
Calcidiol 1-monooxygenase
Cytochrome P450 subfamily XXVIIB polypeptide 1
Cytochrome P450C1 alpha
Cytochrome P450VD1-alpha
Cytochrome p450 27B1
Gene names
Name:Cyp27b1
Synonyms:Cyp27b, Cyp40
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.

Catalytic activity

Calcidiol + NADPH + O2 = calcitriol + NADP+ + H2O.

Cofactor

Heme group By similarity.

Pathway

Hormone biosynthesis; cholecalciferol biosynthesis.

Subcellular location

Mitochondrion membrane.

Tissue specificity

Kidney.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence caution

The sequence BAA22434.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentMembrane
Mitochondrion
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG1 to G0 transition

Inferred from electronic annotation. Source: Ensembl

bone mineralization

Inferred from electronic annotation. Source: Ensembl

calcium ion homeostasis

Inferred from electronic annotation. Source: Ensembl

calcium ion transport

Inferred from mutant phenotype PubMed 12205031. Source: MGI

decidualization

Inferred from electronic annotation. Source: Ensembl

negative regulation of calcidiol 1-monooxygenase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell growth

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of keratinocyte differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of vitamin D 24-hydroxylase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of vitamin D receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

regulation of bone mineralization

Inferred from electronic annotation. Source: Ensembl

response to estrogen

Inferred from electronic annotation. Source: Ensembl

response to interferon-gamma

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to vitamin D

Inferred from direct assay Ref.1. Source: BHF-UCL

vitamin D catabolic process

Inferred from direct assay Ref.1. Source: BHF-UCL

   Cellular_componentmitochondrial membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 14651853PubMed 18614015. Source: MGI

   Molecular_functioncalcidiol 1-monooxygenase activity

Inferred from direct assay Ref.1. Source: BHF-UCL

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 50725-hydroxyvitamin D-1 alpha hydroxylase, mitochondrialPRO_0000003623

Sites

Metal binding4541Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
O35084 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 0669B4478C461B83

FASTA50756,225
        10         20         30         40         50         60 
MTQAVKLASR VFHRIHLPLQ LDASLGSRGS ESVLRSLSDI PGPSTLSFLA ELFCKGGLSR 

        70         80         90        100        110        120 
LHELQVHGAA RYGPIWSGSF GTLRTVYVAD PTLVEQLLRQ ESHCPERCSF SSWAEHRRRH 

       130        140        150        160        170        180 
QRACGLLTAD GEEWQRLRSL LAPLLLRPQA AAGYAGTLDN VVRDLVRRLR RQRGRGSGLP 

       190        200        210        220        230        240 
GLVLDVAGEF YKFGLESIGA VLLGSRLGCL EAEVPPDTET FIHAVGSVFV STLLTMAMPN 

       250        260        270        280        290        300 
WLHHLIPGPW ARLCRDWDQM FAFAQRHVEL REGEAAMRNQ GKPEEDMPSG HHLTHFLFRE 

       310        320        330        340        350        360 
KVSVQSIVGN VTELLLAGVD TVSNTLSWTL YELSRHPDVQ TALHSEITAG TRGSCAHPHG 

       370        380        390        400        410        420 
TALSQLPLLK AVIKEVLRLY PVVPGNSRVP DRDIRVGNYV IPQDTLVSLC HYATSRDPTQ 

       430        440        450        460        470        480 
FPDPNSFNPA RWLGEGPTPH PFASLPFGFG KRSCIGRRLA ELELQMALSQ ILTHFEVLPE 

       490        500 
PGALPIKPMT RTVLVPERSI NLQFVDR 

« Hide

References

[1]"25-hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis."
Takeyama K., Kitanaka S., Sato T., Kobori M., Yanagisawa J., Kato S.
Science 277:1827-1830(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[2]"Cloning of the mouse 25-hydroxyvitamin D3 1-alpha hydroxylase gene."
Kimmel-Jehan C., DeLuca H.F.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 129.
[3]"25-hydroxyvitamin D 1alpha-hydroxylase: structure of the mouse gene, chromosomal assignment, and developmental expression."
Panda D.K., Al-Kawas S., Seldin M.F., Hendy G.N., Goltzman D.
J. Bone Miner. Res. 16:46-56(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB006034 mRNA. Translation: BAA22434.1. Different initiation.
AF235021 Genomic DNA. Translation: AAK15024.1.
AF286219 Genomic DNA. Translation: AAG44889.1.
RefSeqNP_034139.2. NM_010009.2.
UniGeneMm.6216.

3D structure databases

ProteinModelPortalO35084.
SMRO35084. Positions 35-504.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000006907.

PTM databases

PhosphoSiteO35084.

Proteomic databases

PaxDbO35084.
PRIDEO35084.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000165764; ENSMUSP00000130005; ENSMUSG00000006724.
GeneID13115.
KEGGmmu:13115.
UCSCuc007hht.2. mouse.

Organism-specific databases

CTD1594.
MGIMGI:1098274. Cyp27b1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117435.
HOGENOMHOG000253961.
HOVERGENHBG106909.
InParanoidO35084.
KOK07438.
OMALHSEITA.
OrthoDBEOG7ZGX4B.
PhylomeDBO35084.
TreeFamTF105094.

Enzyme and pathway databases

BRENDA1.14.13.13. 3474.
UniPathwayUPA00955.

Gene expression databases

BgeeO35084.
GenevestigatorO35084.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283134.
PROO35084.
SOURCESearch...

Entry information

Entry nameCP27B_MOUSE
AccessionPrimary (citable) accession number: O35084
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot