Reviewed, UniProtKB/Swiss-Prot O35083 (PLCA_MOUSE)

Names and origin

Protein names

Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase alpha
    EC=2.3.1.51
Alternative name(s):
    1-acylglycerol-3-phosphate O-acyltransferase 1
      Short name=1-AGP acyltransferase 1
      Short name=1-AGPAT 1
    Lysophosphatidic acid acyltransferase alpha
      Short name=LPAAT-alpha

Gene names

Name:

Agpat1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	1-acyl-sn-glycerol-3-phosphate acyltransferase alpha		PRO_0000208191
Regions
	Transmembrane	4 – 24	21	Potential
	Transmembrane	35 – 55	21	Potential
	Transmembrane	125 – 145	21	Potential
	Motif	101 – 106	6	HXXXXD motif
Experimental info
	Sequence conflict	73	1	A → V in AAB82009. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O35083-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6227A2C3A09009C1	FASTA	285	31,709
	10 20 30 40 50 60 MELWPGAWTA LLLLLLLLLS TLWFCSSSAK YFFKMAFYNG WILFLAILAI PVCAVRGRNV 70 80 90 100 110 120 ENMKILRLLL LHAKYLYGIR VEVRGAHHFP PTQPYVVVSN HQSSLDLLGM MEVLPDRCVP 130 140 150 160 170 180 IAKRELLWAG SAGLACWLAG IIFIDRKRTG DAISVMSEVA QTLLTQDVRV WVFPEGTRNH 190 200 210 220 230 240 NGSMLPFKRG AFHLAVQAQV PIIPIVMSSY QDFYSKKERR FTSPGRCQVR VLPPVSTEGL 250 260 270 280 TPDDVPALAD SVRHSMLTIF REISTDGLGG GDCLKKPGGA GEARL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of murine 1-acyl-sn-glycerol-3-phosphate acyltransferase." Kume K., Shimizu T. Biochem. Biophys. Res. Commun. 237:663-666(1997) [PubMed: 9299423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed: 14656967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB005623 mRNA. Translation: BAA22599.1. AF030001 Genomic DNA. Translation: AAB82009.1.
IPI	IPI00126548.
PIR	JC5639.
RefSeq	NP_001156851.1. NP_061350.3.
UniGene	Mm.8684
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O35083.
PTM databases
PhosphoSite	O35083.
Proteomic databases
PRIDE	O35083.
Genome annotation databases
Ensembl	ENSMUST00000037489; ENSMUSP00000048573; ENSMUSG00000034254; Mus musculus. [Genome view] ENSMUST00000114140; ENSMUSP00000109776; ENSMUSG00000034254; Mus musculus. [Genome view]
GeneID	55979.
Organism-specific databases
MGI	MGI:1932075. Agpat1.
Phylogenomic databases
HOVERGEN	O35083.
Enzyme and pathway databases
BRENDA	2.3.1.51. 244.
Gene expression databases
ArrayExpress	O35083.
Bgee	O35083.
Genevestigator	O35083.
GermOnline	ENSMUSG00000034254. Mus musculus.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR004552. AGP_acyltrans. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00530. AGP_acyltrn. 1 hit.
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PLCA_MOUSE

Accession

Primary (citable) accession number: O35083
Secondary accession number(s): O35446

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	October 13, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)