Reviewed, UniProtKB/Swiss-Prot O35078 (OXDA_RAT)

Names and origin

Protein names

Recommended name:
    D-amino-acid oxidase
      Short name=DAMOX
      Short name=DAAO
      Short name=DAO
    EC=1.4.3.3

Gene names

Name:	Dao
Synonyms:	Dao1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
Catalytic activity	A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor	FAD.
Subunit structure	Homodimer By similarity.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the DAMOX/DASOX family.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-amino-acid oxidase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	D-amino-acid oxidase		PRO_0000162765
Regions
	Nucleotide binding	3 – 17	15	FAD By similarity
	Nucleotide binding	36 – 37	2	FAD By similarity
	Nucleotide binding	43 – 44	2	FAD By similarity
	Nucleotide binding	48 – 50	3	FAD By similarity
	Nucleotide binding	311 – 315	5	FAD By similarity
	Motif	344 – 346	3	Microbody targeting signal
Sites
	Binding site	52	1	Substrate By similarity
	Binding site	163	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	181	1	FAD By similarity
	Binding site	216	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Binding site	282	1	Substrate By similarity
	Binding site	312	1	Substrate; via carbonyl oxygen By similarity
	Binding site	316	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35078-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2C4697960137A4FE	FASTA	346	38,820
	10 20 30 40 50 60 MRVAVIGAGV IGLSTALCIH ERYHPAQPLH MKIYADRFTP FTTSDVAAGL WQPYLSDPSN 70 80 90 100 110 120 PQEAEWNQQT FDHLQSCLHS PNAEKMGLAL ISGYNLFRDE VPDPFWKSTV LGFRKLTPSE 130 140 150 160 170 180 LDMFPDYSYG WFNTSLLLEG KSYLSWLTER LTERGVKFIH RKVASFEEVV RGGVDVIINC 190 200 210 220 230 240 TGVWAGALQA DASLQPGRGQ IIQVEAPWIK HFILTHDPSL GIYNSPYIIP GSKTVTLGGV 250 260 270 280 290 300 FQLGNWSELN SVHDHNTIWK SCCQLEPTLK NARIMGELTG FRPVRPQVRL ERERLRFGSS 310 320 330 340 SAEVIHNYGH GGYGLTIHWG CAMEAANLFG KILEEKNLSR MPPSHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat D-amino-acid oxidase cDNA: rat D-amino-acid oxidase as an intermediate form between mouse and other mammalian D-amino-acid oxidases." Konno R. Biochim. Biophys. Acta 1395:165-170(1998) [PubMed: 9473656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003400 mRNA. Translation: BAA22840.1. BC088395 mRNA. Translation: AAH88395.1.
IPI	IPI00326225.
RefSeq	NP_446078.1.
UniGene	Rn.81058
3D structure databases
HSSP	HSSP built from PDB template 1AN9 based on UniProtKB P00371.
SMR	O35078. Positions 1-339.
ModBase	Search...
Proteomic databases
PRIDE	O35078.
Genome annotation databases
Ensembl	ENSRNOG00000000694. Rattus norvegicus. [Contig view]
GeneID	114027.
KEGG	rno:114027.
Organism-specific databases
RGD	621138. Dao1.
Phylogenomic databases
HOVERGEN	O35078.
Enzyme and pathway databases
BRENDA	1.4.3.3. 248.
Gene expression databases
ArrayExpress	O35078.
GermOnline	ENSRNOG00000000694. Rattus norvegicus.
Family and domain databases
InterPro	IPR006181. D-amino_acid_oxidase_CS. IPR006076. FAD-dep_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PROSITE	PS00677. DAO. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618183.

Entry information

Entry name

OXDA_RAT

Accession

Primary (citable) accession number: O35078

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)