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Protein

D-amino-acid oxidase

Gene

Dao

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.

Catalytic activityi

A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521SubstrateBy similarity
Binding sitei163 – 1631FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei181 – 1811FADBy similarity
Binding sitei216 – 2161SubstrateBy similarity
Binding sitei227 – 2271SubstrateBy similarity
Binding sitei282 – 2821SubstrateBy similarity
Binding sitei312 – 3121Substrate; via carbonyl oxygenBy similarity
Binding sitei316 – 3161FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi3 – 1715FADBy similarityAdd
BLAST
Nucleotide bindingi36 – 372FADBy similarity
Nucleotide bindingi43 – 442FADBy similarity
Nucleotide bindingi48 – 503FADBy similarity
Nucleotide bindingi311 – 3155FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.4.3.3. 5301.
SABIO-RKO35078.

Names & Taxonomyi

Protein namesi
Recommended name:
D-amino-acid oxidase (EC:1.4.3.3)
Short name:
DAAO
Short name:
DAMOX
Short name:
DAO
Gene namesi
Name:Dao
Synonyms:Dao1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621138. Dao.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5756.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346D-amino-acid oxidasePRO_0000162765Add
BLAST

Proteomic databases

PaxDbiO35078.
PRIDEiO35078.

PTM databases

iPTMnetiO35078.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiO35078. 1 interaction.
MINTiMINT-4582363.
STRINGi10116.ENSRNOP00000000881.

Chemistry

BindingDBiO35078.

Structurei

3D structure databases

ProteinModelPortaliO35078.
SMRiO35078. Positions 1-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi344 – 3463Microbody targeting signal

Sequence similaritiesi

Belongs to the DAMOX/DASOX family.Curated

Phylogenomic databases

eggNOGiKOG3923. Eukaryota.
COG0665. LUCA.
HOGENOMiHOG000046303.
HOVERGENiHBG003493.
InParanoidiO35078.
KOiK00273.
OMAiIHEHYHS.
OrthoDBiEOG7B31NB.
PhylomeDBiO35078.
TreeFamiTF313887.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR006181. D-amino_acid_oxidase_CS.
IPR023209. DAO.
IPR006076. FAD-dep_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11530. PTHR11530. 1 hit.
PfamiPF01266. DAO. 1 hit.
[Graphical view]
PIRSFiPIRSF000189. D-aa_oxidase. 1 hit.
PROSITEiPS00677. DAO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVAVIGAGV IGLSTALCIH ERYHPAQPLH MKIYADRFTP FTTSDVAAGL
60 70 80 90 100
WQPYLSDPSN PQEAEWNQQT FDHLQSCLHS PNAEKMGLAL ISGYNLFRDE
110 120 130 140 150
VPDPFWKSTV LGFRKLTPSE LDMFPDYSYG WFNTSLLLEG KSYLSWLTER
160 170 180 190 200
LTERGVKFIH RKVASFEEVV RGGVDVIINC TGVWAGALQA DASLQPGRGQ
210 220 230 240 250
IIQVEAPWIK HFILTHDPSL GIYNSPYIIP GSKTVTLGGV FQLGNWSELN
260 270 280 290 300
SVHDHNTIWK SCCQLEPTLK NARIMGELTG FRPVRPQVRL ERERLRFGSS
310 320 330 340
SAEVIHNYGH GGYGLTIHWG CAMEAANLFG KILEEKNLSR MPPSHL
Length:346
Mass (Da):38,820
Last modified:January 1, 1998 - v1
Checksum:i2C4697960137A4FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003400 mRNA. Translation: BAA22840.1.
BC088395 mRNA. Translation: AAH88395.1.
RefSeqiNP_446078.1. NM_053626.1.
UniGeneiRn.81058.

Genome annotation databases

GeneIDi114027.
KEGGirno:114027.
UCSCiRGD:621138. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003400 mRNA. Translation: BAA22840.1.
BC088395 mRNA. Translation: AAH88395.1.
RefSeqiNP_446078.1. NM_053626.1.
UniGeneiRn.81058.

3D structure databases

ProteinModelPortaliO35078.
SMRiO35078. Positions 1-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35078. 1 interaction.
MINTiMINT-4582363.
STRINGi10116.ENSRNOP00000000881.

Chemistry

BindingDBiO35078.
ChEMBLiCHEMBL5756.

PTM databases

iPTMnetiO35078.

Proteomic databases

PaxDbiO35078.
PRIDEiO35078.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114027.
KEGGirno:114027.
UCSCiRGD:621138. rat.

Organism-specific databases

CTDi1610.
RGDi621138. Dao.

Phylogenomic databases

eggNOGiKOG3923. Eukaryota.
COG0665. LUCA.
HOGENOMiHOG000046303.
HOVERGENiHBG003493.
InParanoidiO35078.
KOiK00273.
OMAiIHEHYHS.
OrthoDBiEOG7B31NB.
PhylomeDBiO35078.
TreeFamiTF313887.

Enzyme and pathway databases

BRENDAi1.4.3.3. 5301.
SABIO-RKO35078.

Miscellaneous databases

PROiO35078.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR006181. D-amino_acid_oxidase_CS.
IPR023209. DAO.
IPR006076. FAD-dep_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11530. PTHR11530. 1 hit.
PfamiPF01266. DAO. 1 hit.
[Graphical view]
PIRSFiPIRSF000189. D-aa_oxidase. 1 hit.
PROSITEiPS00677. DAO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat D-amino-acid oxidase cDNA: rat D-amino-acid oxidase as an intermediate form between mouse and other mammalian D-amino-acid oxidases."
    Konno R.
    Biochim. Biophys. Acta 1395:165-170(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiOXDA_RAT
AccessioniPrimary (citable) accession number: O35078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.