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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

Gpd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41NADBy similarity1
Binding sitei97NADBy similarity1
Binding sitei120SubstrateBy similarity1
Binding sitei153NAD; via amide nitrogenBy similarity1
Active sitei204Proton acceptorSequence analysis1
Binding sitei269NADBy similarity1
Binding sitei296NAD; via amide nitrogenBy similarity1
Binding sitei298NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15NADBy similarity6

GO - Molecular functioni

  • glycerol-3-phosphate dehydrogenase [NAD+] activity Source: RGD
  • glycerol-3-phosphate dehydrogenase activity Source: RGD
  • NAD binding Source: RGD
  • protein homodimerization activity Source: InterPro

GO - Biological processi

  • carbohydrate metabolic process Source: RGD
  • cellular response to cAMP Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • gluconeogenesis Source: RGD
  • glycerol-3-phosphate catabolic process Source: InterPro
  • glycerol-3-phosphate metabolic process Source: RGD
  • glycerolipid metabolic process Source: RGD
  • glycerophosphate shuttle Source: RGD
  • NADH metabolic process Source: RGD
  • NADH oxidation Source: RGD
  • positive regulation of glycolytic process Source: RGD

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

ReactomeiR-RNO-1483166. Synthesis of PA.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
Short name:
GPD-C
Short name:
GPDH-C
Gene namesi
Name:Gpd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi621381. Gpd1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3971.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001380821 – 349Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicAdd BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei154PhosphoserineBy similarity1
Modified residuei289N6-succinyllysineBy similarity1
Modified residuei326PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35077.
PRIDEiO35077.

PTM databases

iPTMnetiO35077.
PhosphoSitePlusiO35077.

Expressioni

Gene expression databases

BgeeiENSRNOG00000056457.
GenevisibleiO35077. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248887. 1 interactor.
MINTiMINT-4566686.
STRINGi10116.ENSRNOP00000026200.

Structurei

3D structure databases

ProteinModelPortaliO35077.
SMRiO35077.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni269 – 270Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2711. Eukaryota.
COG0240. LUCA.
GeneTreeiENSGT00390000003114.
HOGENOMiHOG000246855.
HOVERGENiHBG003669.
InParanoidiO35077.
KOiK00006.
OMAiNMIGKGY.
OrthoDBiEOG091G0DO6.
PhylomeDBiO35077.
TreeFamiTF300836.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
InterProiView protein in InterPro
IPR008927. 6-PGluconate_DH-like_C_sf.
IPR013328. 6PGD_dom2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiView protein in PROSITE
PS00957. NAD_G3PDH. 1 hit.

Sequencei

Sequence statusi: Complete.

O35077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKKVCIVG SGNWGSAIAK IVGSNASQLA HFDPRVTMWV FEEDIGGRKL
60 70 80 90 100
TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAATGADILV FVVPHQFIGK
110 120 130 140 150
ICDQLKGHLK ANTIGISLIK GIDEGPNGLK LISEVIGESL GIPMSVLMGA
160 170 180 190 200
NIASEVAEEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC
210 220 230 240 250
GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGSVSSA
260 270 280 290 300
TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP
310 320 330 340
QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIC CLQNHPEHM
Length:349
Mass (Da):37,453
Last modified:January 23, 2007 - v4
Checksum:i26208A55DC033A9A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179E → D in BAA21763 (PubMed:9237667).Curated1
Sequence conflicti238F → S in BAA21763 (PubMed:9237667).Curated1
Sequence conflicti338F → I in BAA21763 (PubMed:9237667).Curated1
Sequence conflicti340C → R in BAA21763 (PubMed:9237667).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002558 mRNA. Translation: BAA21763.1.
BC088396 mRNA. Translation: AAH88396.1.
RefSeqiNP_071551.2. NM_022215.2.
XP_006257438.1. XM_006257376.2.
UniGeneiRn.44452.

Genome annotation databases

EnsembliENSRNOT00000087662; ENSRNOP00000074647; ENSRNOG00000056457.
GeneIDi60666.
KEGGirno:60666.
UCSCiRGD:621381. rat.

Similar proteinsi

Entry informationi

Entry nameiGPDA_RAT
AccessioniPrimary (citable) accession number: O35077
Secondary accession number(s): Q5I0F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 140 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families