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O35077

- GPDA_RAT

UniProt

O35077 - GPDA_RAT

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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

Gpd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411NADBy similarity
Binding sitei97 – 971NADBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei153 – 1531NAD; via amide nitrogenBy similarity
Active sitei204 – 2041Proton acceptorSequence Analysis
Binding sitei269 – 2691NADBy similarity
Binding sitei296 – 2961NAD; via amide nitrogenBy similarity
Binding sitei298 – 2981NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADBy similarity

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: RGD
  2. glycerol-3-phosphate dehydrogenase activity Source: RGD
  3. NAD binding Source: RGD

GO - Biological processi

  1. carbohydrate metabolic process Source: RGD
  2. cellular response to cAMP Source: Ensembl
  3. cellular response to tumor necrosis factor Source: Ensembl
  4. gluconeogenesis Source: Ensembl
  5. glycerol-3-phosphate catabolic process Source: InterPro
  6. glycerol-3-phosphate metabolic process Source: RGD
  7. glycerolipid metabolic process Source: RGD
  8. glycerophosphate shuttle Source: Ensembl
  9. NADH metabolic process Source: RGD
  10. NADH oxidation Source: RGD
  11. positive regulation of glycolytic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_194934. Synthesis of PA.
REACT_232174. Triglyceride Biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
Short name:
GPD-C
Short name:
GPDH-C
Gene namesi
Name:Gpd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome X

Organism-specific databases

RGDi621381. Gpd1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RGD
  2. extracellular vesicular exosome Source: Ensembl
  3. glycerol-3-phosphate dehydrogenase complex Source: InterPro
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000138082Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei289 – 2891N6-succinyllysineBy similarity

Proteomic databases

PaxDbiO35077.
PRIDEiO35077.

Expressioni

Gene expression databases

GenevestigatoriO35077.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi248887. 1 interaction.
MINTiMINT-4566686.
STRINGi10116.ENSRNOP00000026200.

Structurei

3D structure databases

ProteinModelPortaliO35077.
SMRiO35077. Positions 1-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni269 – 2702Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0240.
GeneTreeiENSGT00390000003114.
HOGENOMiHOG000246855.
HOVERGENiHBG003669.
InParanoidiO35077.
KOiK00006.
OMAiPVGEFIH.
OrthoDBiEOG7ZKSBS.
PhylomeDBiO35077.
TreeFamiTF300836.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35077-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGKKVCIVG SGNWGSAIAK IVGSNASQLA HFDPRVTMWV FEEDIGGRKL
60 70 80 90 100
TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAATGADILV FVVPHQFIGK
110 120 130 140 150
ICDQLKGHLK ANTIGISLIK GIDEGPNGLK LISEVIGESL GIPMSVLMGA
160 170 180 190 200
NIASEVAEEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC
210 220 230 240 250
GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGSVSSA
260 270 280 290 300
TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP
310 320 330 340
QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIC CLQNHPEHM
Length:349
Mass (Da):37,453
Last modified:January 23, 2007 - v4
Checksum:i26208A55DC033A9A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791E → D in BAA21763. (PubMed:9237667)Curated
Sequence conflicti238 – 2381F → S in BAA21763. (PubMed:9237667)Curated
Sequence conflicti338 – 3381F → I in BAA21763. (PubMed:9237667)Curated
Sequence conflicti340 – 3401C → R in BAA21763. (PubMed:9237667)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002558 mRNA. Translation: BAA21763.1.
BC088396 mRNA. Translation: AAH88396.1.
RefSeqiNP_071551.2. NM_022215.2.
XP_006257438.1. XM_006257376.1.
UniGeneiRn.44452.

Genome annotation databases

EnsembliENSRNOT00000026199; ENSRNOP00000026200; ENSRNOG00000019213.
GeneIDi60666.
KEGGirno:60666.
UCSCiRGD:621381. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002558 mRNA. Translation: BAA21763.1 .
BC088396 mRNA. Translation: AAH88396.1 .
RefSeqi NP_071551.2. NM_022215.2.
XP_006257438.1. XM_006257376.1.
UniGenei Rn.44452.

3D structure databases

ProteinModelPortali O35077.
SMRi O35077. Positions 1-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248887. 1 interaction.
MINTi MINT-4566686.
STRINGi 10116.ENSRNOP00000026200.

Chemistry

ChEMBLi CHEMBL3971.

Proteomic databases

PaxDbi O35077.
PRIDEi O35077.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000026199 ; ENSRNOP00000026200 ; ENSRNOG00000019213 .
GeneIDi 60666.
KEGGi rno:60666.
UCSCi RGD:621381. rat.

Organism-specific databases

CTDi 2819.
RGDi 621381. Gpd1.

Phylogenomic databases

eggNOGi COG0240.
GeneTreei ENSGT00390000003114.
HOGENOMi HOG000246855.
HOVERGENi HBG003669.
InParanoidi O35077.
KOi K00006.
OMAi PVGEFIH.
OrthoDBi EOG7ZKSBS.
PhylomeDBi O35077.
TreeFami TF300836.

Enzyme and pathway databases

Reactomei REACT_194934. Synthesis of PA.
REACT_232174. Triglyceride Biosynthesis.

Miscellaneous databases

NextBioi 612385.
PROi O35077.

Gene expression databases

Genevestigatori O35077.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11728. PTHR11728. 1 hit.
Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSi PR00077. GPDHDRGNASE.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
PROSITEi PS00957. NAD_G3PDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure."
    Daikoku T., Shinohara Y., Shima A., Yamazaki N., Terada H.
    FEBS Lett. 410:383-386(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: White adipose tissue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 6-48; 21-35; 70-101; 188-224 AND 241-270, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiGPDA_RAT
AccessioniPrimary (citable) accession number: O35077
Secondary accession number(s): Q5I0F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 117 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3