Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic

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Reviewed, UniProtKB/Swiss-Prot O35077 (GPDA_RAT)

Last modified June 16, 2009. Version 69. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
      Short name=GPDH-C
      Short name=GPD-C
    EC=1.1.1.8

Gene names

Name:

Gpd1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD⁺ = glycerone phosphate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

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Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	NADH oxidation Inferred from direct assay. Source: RGD glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro glycerolipid metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: RGD glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro soluble fraction Inferred from direct assay. Source: RGD
Molecular function	NAD or NADH binding Inferred from direct assay. Source: RGD glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from direct assay. Source: RGD glycerol-3-phosphate dehydrogenase activity Inferred from direct assay. Source: RGD protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 349

348

Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic

PRO_0000138082

Regions

Nucleotide binding

10 – 15

NAD By similarity

Region

269 – 270

Substrate binding By similarity

Sites

Active site

204

Proton acceptor Potential

Binding site

NAD By similarity

Binding site

NAD By similarity

Binding site

120

Substrate By similarity

Binding site

153

NAD; via amide nitrogen By similarity

Binding site

269

NAD By similarity

Binding site

296

NAD; via amide nitrogen By similarity

Binding site

298

NAD By similarity

Experimental info

Sequence conflict

179

E → D in BAA21763. Ref.1

Sequence conflict

238

F → S in BAA21763. Ref.1

Sequence conflict

338

F → I in BAA21763. Ref.1

Sequence conflict

340

C → R in BAA21763. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

O35077-1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 26208A55DC033A9A
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FASTA

349

37,453

        10         20         30         40         50         60 
MAGKKVCIVG SGNWGSAIAK IVGSNASQLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN 

        70         80         90        100        110        120 
VKYLPGHKLP PNVVAVPDVV QAATGADILV FVVPHQFIGK ICDQLKGHLK ANTIGISLIK 

       130        140        150        160        170        180 
GIDEGPNGLK LISEVIGESL GIPMSVLMGA NIASEVAEEK FCETTIGCKD PAQGQLLKEL 

       190        200        210        220        230        240 
MQTPNFRITV VQEVDTVEIC GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK 

       250        260        270        280        290        300 
LFCSGSVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 

       310        320        330        340 
QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIC CLQNHPEHM

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure." Daikoku T., Shinohara Y., Shima A., Yamazaki N., Terada H. FEBS Lett. 410:383-386(1997) [PubMed: 9237667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: White adipose tissue.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 6-48; 21-35; 70-101; 188-224 AND 241-270, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.

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Cross-references

Sequence databases
	AB002558 mRNA. Translation: BAA21763.1. BC088396 mRNA. Translation: AAH88396.1.
IPI	IPI00231148.
RefSeq	NP_071551.2.
UniGene	Rn.44452
3D structure databases
HSSP	HSSP built from PDB template 1EVY based on UniProtKB P90551.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000019213. Rattus norvegicus. [Contig view]
GeneID	60666.
KEGG	rno:60666.
NMPDR	fig\|10116.3.peg.27756.
Organism-specific databases
RGD	621381. Gpd1.
Phylogenomic databases
HOVERGEN	O35077.
OMA	O35077. THENVKY.
Enzyme and pathway databases
BRENDA	1.1.1.8. 248.
Gene expression databases
ArrayExpress	O35077.
GermOnline	ENSRNOG00000019213. Rattus norvegicus.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	612385.

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Entry information

Entry name

GPDA_RAT

Accession

Primary (citable) accession number: O35077
Secondary accession number(s): Q5I0F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 69 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents