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O35077 (GPDA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Short name=GPD-C
Short name=GPDH-C
EC=1.1.1.8
Gene names
Name:Gpd1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processNADH metabolic process

Inferred from direct assay PubMed 12351438. Source: RGD

NADH oxidation

Inferred from direct assay PubMed 9550546. Source: RGD

carbohydrate metabolic process

Inferred from direct assay PubMed 1834654. Source: RGD

cellular response to cAMP

Inferred from electronic annotation. Source: Ensembl

cellular response to tumor necrosis factor

Inferred from electronic annotation. Source: Ensembl

gluconeogenesis

Inferred from electronic annotation. Source: Ensembl

glycerol-3-phosphate catabolic process

Inferred from electronic annotation. Source: InterPro

glycerol-3-phosphate metabolic process

Inferred from direct assay PubMed 12351438. Source: RGD

glycerolipid metabolic process

Inferred from direct assay PubMed 2997397. Source: RGD

   Cellular_componentcytosol

Inferred from direct assay PubMed 1834654. Source: RGD

glycerol-3-phosphate dehydrogenase complex

Inferred from electronic annotation. Source: InterPro

mitochondrion

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionNAD binding

Inferred from direct assay PubMed 9550546. Source: RGD

glycerol-3-phosphate dehydrogenase [NAD+] activity

Inferred from direct assay PubMed 12351438PubMed 9550546. Source: RGD

glycerol-3-phosphate dehydrogenase activity

Inferred from direct assay PubMed 1834654. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
PRO_0000138082

Regions

Nucleotide binding10 – 156NAD By similarity
Region269 – 2702Substrate binding By similarity

Sites

Active site2041Proton acceptor Potential
Binding site411NAD By similarity
Binding site971NAD By similarity
Binding site1201Substrate By similarity
Binding site1531NAD; via amide nitrogen By similarity
Binding site2691NAD By similarity
Binding site2961NAD; via amide nitrogen By similarity
Binding site2981NAD By similarity

Amino acid modifications

Modified residue2891N6-succinyllysine By similarity

Experimental info

Sequence conflict1791E → D in BAA21763. Ref.1
Sequence conflict2381F → S in BAA21763. Ref.1
Sequence conflict3381F → I in BAA21763. Ref.1
Sequence conflict3401C → R in BAA21763. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O35077 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 26208A55DC033A9A

FASTA34937,453
        10         20         30         40         50         60 
MAGKKVCIVG SGNWGSAIAK IVGSNASQLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN 

        70         80         90        100        110        120 
VKYLPGHKLP PNVVAVPDVV QAATGADILV FVVPHQFIGK ICDQLKGHLK ANTIGISLIK 

       130        140        150        160        170        180 
GIDEGPNGLK LISEVIGESL GIPMSVLMGA NIASEVAEEK FCETTIGCKD PAQGQLLKEL 

       190        200        210        220        230        240 
MQTPNFRITV VQEVDTVEIC GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK 

       250        260        270        280        290        300 
LFCSGSVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 

       310        320        330        340 
QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIC CLQNHPEHM 

« Hide

References

« Hide 'large scale' references
[1]"Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure."
Daikoku T., Shinohara Y., Shima A., Yamazaki N., Terada H.
FEBS Lett. 410:383-386(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: White adipose tissue.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]Lubec G., Afjehi-Sadat L., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 6-48; 21-35; 70-101; 188-224 AND 241-270, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB002558 mRNA. Translation: BAA21763.1.
BC088396 mRNA. Translation: AAH88396.1.
RefSeqNP_071551.2. NM_022215.2.
XP_006257438.1. XM_006257376.1.
UniGeneRn.44452.

3D structure databases

ProteinModelPortalO35077.
SMRO35077. Positions 1-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248887. 1 interaction.
MINTMINT-4566686.
STRING10116.ENSRNOP00000026200.

Chemistry

ChEMBLCHEMBL3971.

Proteomic databases

PaxDbO35077.
PRIDEO35077.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026199; ENSRNOP00000026200; ENSRNOG00000019213.
GeneID60666.
KEGGrno:60666.
UCSCRGD:621381. rat.

Organism-specific databases

CTD2819.
RGD621381. Gpd1.

Phylogenomic databases

eggNOGCOG0240.
GeneTreeENSGT00390000003114.
HOGENOMHOG000246855.
HOVERGENHBG003669.
InParanoidO35077.
KOK00006.
OMAHILPFAR.
OrthoDBEOG7ZKSBS.
PhylomeDBO35077.
TreeFamTF300836.

Gene expression databases

GenevestigatorO35077.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR03376. glycerol3P_DH. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio612385.
PROO35077.

Entry information

Entry nameGPDA_RAT
AccessionPrimary (citable) accession number: O35077
Secondary accession number(s): Q5I0F4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 112 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families