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O35077

- GPDA_RAT

UniProt

O35077 - GPDA_RAT

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

Gpd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411NADBy similarity
    Binding sitei97 – 971NADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei153 – 1531NAD; via amide nitrogenBy similarity
    Active sitei204 – 2041Proton acceptorSequence Analysis
    Binding sitei269 – 2691NADBy similarity
    Binding sitei296 – 2961NAD; via amide nitrogenBy similarity
    Binding sitei298 – 2981NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: RGD
    2. glycerol-3-phosphate dehydrogenase activity Source: RGD
    3. NAD binding Source: RGD

    GO - Biological processi

    1. carbohydrate metabolic process Source: RGD
    2. cellular response to cAMP Source: Ensembl
    3. cellular response to tumor necrosis factor Source: Ensembl
    4. gluconeogenesis Source: Ensembl
    5. glycerol-3-phosphate catabolic process Source: InterPro
    6. glycerol-3-phosphate metabolic process Source: RGD
    7. glycerolipid metabolic process Source: RGD
    8. NADH metabolic process Source: RGD
    9. NADH oxidation Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_194934. Synthesis of PA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
    Short name:
    GPD-C
    Short name:
    GPDH-C
    Gene namesi
    Name:Gpd1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome X

    Organism-specific databases

    RGDi621381. Gpd1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. glycerol-3-phosphate dehydrogenase complex Source: InterPro
    3. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000138082Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei289 – 2891N6-succinyllysineBy similarity

    Proteomic databases

    PaxDbiO35077.
    PRIDEiO35077.

    Expressioni

    Gene expression databases

    GenevestigatoriO35077.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi248887. 1 interaction.
    MINTiMINT-4566686.
    STRINGi10116.ENSRNOP00000026200.

    Structurei

    3D structure databases

    ProteinModelPortaliO35077.
    SMRiO35077. Positions 1-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni269 – 2702Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0240.
    GeneTreeiENSGT00390000003114.
    HOGENOMiHOG000246855.
    HOVERGENiHBG003669.
    InParanoidiO35077.
    KOiK00006.
    OMAiPVGEFIH.
    OrthoDBiEOG7ZKSBS.
    PhylomeDBiO35077.
    TreeFamiTF300836.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O35077-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGKKVCIVG SGNWGSAIAK IVGSNASQLA HFDPRVTMWV FEEDIGGRKL    50
    TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAATGADILV FVVPHQFIGK 100
    ICDQLKGHLK ANTIGISLIK GIDEGPNGLK LISEVIGESL GIPMSVLMGA 150
    NIASEVAEEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC 200
    GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGSVSSA 250
    TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 300
    QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIC CLQNHPEHM 349
    Length:349
    Mass (Da):37,453
    Last modified:January 23, 2007 - v4
    Checksum:i26208A55DC033A9A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti179 – 1791E → D in BAA21763. (PubMed:9237667)Curated
    Sequence conflicti238 – 2381F → S in BAA21763. (PubMed:9237667)Curated
    Sequence conflicti338 – 3381F → I in BAA21763. (PubMed:9237667)Curated
    Sequence conflicti340 – 3401C → R in BAA21763. (PubMed:9237667)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB002558 mRNA. Translation: BAA21763.1.
    BC088396 mRNA. Translation: AAH88396.1.
    RefSeqiNP_071551.2. NM_022215.2.
    XP_006257438.1. XM_006257376.1.
    UniGeneiRn.44452.

    Genome annotation databases

    EnsembliENSRNOT00000026199; ENSRNOP00000026200; ENSRNOG00000019213.
    GeneIDi60666.
    KEGGirno:60666.
    UCSCiRGD:621381. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB002558 mRNA. Translation: BAA21763.1 .
    BC088396 mRNA. Translation: AAH88396.1 .
    RefSeqi NP_071551.2. NM_022215.2.
    XP_006257438.1. XM_006257376.1.
    UniGenei Rn.44452.

    3D structure databases

    ProteinModelPortali O35077.
    SMRi O35077. Positions 1-349.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248887. 1 interaction.
    MINTi MINT-4566686.
    STRINGi 10116.ENSRNOP00000026200.

    Chemistry

    ChEMBLi CHEMBL3971.

    Proteomic databases

    PaxDbi O35077.
    PRIDEi O35077.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000026199 ; ENSRNOP00000026200 ; ENSRNOG00000019213 .
    GeneIDi 60666.
    KEGGi rno:60666.
    UCSCi RGD:621381. rat.

    Organism-specific databases

    CTDi 2819.
    RGDi 621381. Gpd1.

    Phylogenomic databases

    eggNOGi COG0240.
    GeneTreei ENSGT00390000003114.
    HOGENOMi HOG000246855.
    HOVERGENi HBG003669.
    InParanoidi O35077.
    KOi K00006.
    OMAi PVGEFIH.
    OrthoDBi EOG7ZKSBS.
    PhylomeDBi O35077.
    TreeFami TF300836.

    Enzyme and pathway databases

    Reactomei REACT_194934. Synthesis of PA.

    Miscellaneous databases

    NextBioi 612385.
    PROi O35077.

    Gene expression databases

    Genevestigatori O35077.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure."
      Daikoku T., Shinohara Y., Shima A., Yamazaki N., Terada H.
      FEBS Lett. 410:383-386(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: White adipose tissue.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 6-48; 21-35; 70-101; 188-224 AND 241-270, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiGPDA_RAT
    AccessioniPrimary (citable) accession number: O35077
    Secondary accession number(s): Q5I0F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3