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O35074

- PTGIS_MOUSE

UniProt

O35074 - PTGIS_MOUSE

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Protein
Prostacyclin synthase
Gene
Ptgis, Cyp8
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi442 – 4421Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. heme binding Source: RefGenome
  2. iron ion binding Source: InterPro
  3. monooxygenase activity Source: InterPro
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro
  5. prostaglandin-I synthase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. apoptotic signaling pathway Source: Ensembl
  2. cellular response to hypoxia Source: UniProtKB
  3. cellular response to interleukin-1 Source: UniProtKB
  4. cellular response to interleukin-6 Source: UniProtKB
  5. negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
  6. negative regulation of inflammatory response Source: UniProtKB
  7. negative regulation of nitric oxide biosynthetic process Source: UniProtKB
  8. positive regulation of angiogenesis Source: UniProtKB
  9. positive regulation of execution phase of apoptosis Source: UniProtKB
  10. positive regulation of peroxisome proliferator activated receptor signaling pathway Source: UniProtKB
  11. prostaglandin biosynthetic process Source: UniProtKB
  12. regulation of apoptotic process Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_188623. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin synthase (EC:5.3.99.4)
Alternative name(s):
Prostaglandin I2 synthase
Gene namesi
Name:Ptgis
Synonyms:Cyp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1097156. Ptgis.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. caveola Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. extracellular space Source: UniProtKB
  5. integral component of membrane Source: UniProtKB-KW
  6. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Prostacyclin synthase
PRO_0000051911Add
BLAST

Proteomic databases

MaxQBiO35074.
PaxDbiO35074.
PRIDEiO35074.

PTM databases

PhosphoSiteiO35074.

Expressioni

Gene expression databases

ArrayExpressiO35074.
BgeeiO35074.
CleanExiMM_PTGIS.
GenevestigatoriO35074.

Interactioni

Protein-protein interaction databases

IntActiO35074. 1 interaction.
MINTiMINT-4109067.
STRINGi10090.ENSMUSP00000085357.

Structurei

3D structure databases

ProteinModelPortaliO35074.
SMRiO35074. Positions 24-500.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
KOiK01831.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR027286. PTGIS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PIRSF500628. PTGIS. 1 hit.
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

O35074-1 [UniParc]FASTAAdd to Basket

« Hide

MSWAALLGLL AVLLLLLLLL SRRRARRPGE PPLDLGSIPW LGHALEFGRD    50
AASFLTRMKE KHGDIFTVLV GGRYVTVLLD PHSYDTVVWE LRTRLDFHPY 100
AIFLMERIFD LQLPNFNPSE EKARMKPTLM HRDLQALTEA MYTNLRTVLL 150
GDSTEAGSGW QETGLLEFSY NALLSAGYLT LYGVEASPRT HESQAQDRVH 200
SADVFHTFRQ LDLLLPKLAR GSLSAGDKDH ACSVKNRLWK LLSPARLASR 250
ADRSSWLESY LRHLEEMGVS EEMQARALVL QLWATQGNMG PTAFWLLLFL 300
LKNPEALAAV RAELKHTVWQ AEQPVSQMTT LPQKILDSMP VLDSVLNETL 350
RLTAAPFITR EVMADLALPM ADGREFSLRR GDRLLLFPFL SPQKDPEIYT 400
EPEVFKYNRF LNPDGSEKKD FYKDGKRLKN YNMPWGAGHN QCLGKSYAIN 450
SIKQFVVLLL THFDLELGSE DTEVPEFDLS RYGFGLMQPE EDVPIRYRAR 500
L 501
Length:501
Mass (Da):57,047
Last modified:January 1, 1998 - v1
Checksum:iF018F85D3A1B0EBB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001607 mRNA. Translation: BAA21717.1.
AK076585 mRNA. Translation: BAC36403.1.
BC062151 mRNA. Translation: AAH62151.1.
CCDSiCCDS17097.1.
RefSeqiNP_032994.1. NM_008968.3.
UniGeneiMm.2339.

Genome annotation databases

EnsembliENSMUST00000018113; ENSMUSP00000018113; ENSMUSG00000017969.
GeneIDi19223.
KEGGimmu:19223.
UCSCiuc008nzl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001607 mRNA. Translation: BAA21717.1 .
AK076585 mRNA. Translation: BAC36403.1 .
BC062151 mRNA. Translation: AAH62151.1 .
CCDSi CCDS17097.1.
RefSeqi NP_032994.1. NM_008968.3.
UniGenei Mm.2339.

3D structure databases

ProteinModelPortali O35074.
SMRi O35074. Positions 24-500.
ModBasei Search...

Protein-protein interaction databases

IntActi O35074. 1 interaction.
MINTi MINT-4109067.
STRINGi 10090.ENSMUSP00000085357.

PTM databases

PhosphoSitei O35074.

Proteomic databases

MaxQBi O35074.
PaxDbi O35074.
PRIDEi O35074.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000018113 ; ENSMUSP00000018113 ; ENSMUSG00000017969 .
GeneIDi 19223.
KEGGi mmu:19223.
UCSCi uc008nzl.1. mouse.

Organism-specific databases

CTDi 5740.
MGIi MGI:1097156. Ptgis.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00550000074551.
HOGENOMi HOG000231026.
HOVERGENi HBG051100.
KOi K01831.
TreeFami TF105090.

Enzyme and pathway databases

Reactomei REACT_188623. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Miscellaneous databases

NextBioi 296004.
PROi O35074.
SOURCEi Search...

Gene expression databases

ArrayExpressi O35074.
Bgeei O35074.
CleanExi MM_PTGIS.
Genevestigatori O35074.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR027286. PTGIS.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PIRSFi PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PIRSF500628. PTGIS. 1 hit.
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Inverse gene expression of prostacyclin and thromboxane synthases in resident and activated peritoneal macrophages."
    Kuwamoto S., Inoue H., Tone Y., Izumi Y., Tanabe T.
    FEBS Lett. 409:242-246(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Peritoneal macrophage.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Limb.

Entry informationi

Entry nameiPTGIS_MOUSE
AccessioniPrimary (citable) accession number: O35074
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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