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Protein

Prostacyclin synthase

Gene

Ptgis

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).By similarity

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107SubstrateBy similarity1
Binding sitei113Substrate; via amide nitrogenBy similarity1
Binding sitei288SubstrateBy similarity1
Binding sitei383SubstrateBy similarity1
Metal bindingi442Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-197264. Nicotinamide salvaging.
R-MMU-211979. Eicosanoids.
R-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin synthase (EC:5.3.99.4By similarity)
Alternative name(s):
Prostaglandin I2 synthase
Gene namesi
Name:Ptgis
Synonyms:Cyp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1097156. Ptgis.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519111 – 501Prostacyclin synthaseAdd BLAST501

Proteomic databases

MaxQBiO35074.
PaxDbiO35074.
PeptideAtlasiO35074.
PRIDEiO35074.

PTM databases

iPTMnetiO35074.
PhosphoSitePlusiO35074.

Expressioni

Gene expression databases

BgeeiENSMUSG00000017969.
CleanExiMM_PTGIS.
ExpressionAtlasiO35074. baseline and differential.
GenevisibleiO35074. MM.

Interactioni

Protein-protein interaction databases

IntActiO35074. 1 interactor.
MINTiMINT-4109067.
STRINGi10090.ENSMUSP00000018113.

Structurei

3D structure databases

ProteinModelPortaliO35074.
SMRiO35074.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni359 – 360Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiO35074.
KOiK01831.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR036396. Cyt_P450_sf.
IPR027286. PTGIS.
PANTHERiPTHR24306:SF4. PTHR24306:SF4. 1 hit.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PIRSF500628. PTGIS. 1 hit.
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

O35074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWAALLGLL AVLLLLLLLL SRRRARRPGE PPLDLGSIPW LGHALEFGRD
60 70 80 90 100
AASFLTRMKE KHGDIFTVLV GGRYVTVLLD PHSYDTVVWE LRTRLDFHPY
110 120 130 140 150
AIFLMERIFD LQLPNFNPSE EKARMKPTLM HRDLQALTEA MYTNLRTVLL
160 170 180 190 200
GDSTEAGSGW QETGLLEFSY NALLSAGYLT LYGVEASPRT HESQAQDRVH
210 220 230 240 250
SADVFHTFRQ LDLLLPKLAR GSLSAGDKDH ACSVKNRLWK LLSPARLASR
260 270 280 290 300
ADRSSWLESY LRHLEEMGVS EEMQARALVL QLWATQGNMG PTAFWLLLFL
310 320 330 340 350
LKNPEALAAV RAELKHTVWQ AEQPVSQMTT LPQKILDSMP VLDSVLNETL
360 370 380 390 400
RLTAAPFITR EVMADLALPM ADGREFSLRR GDRLLLFPFL SPQKDPEIYT
410 420 430 440 450
EPEVFKYNRF LNPDGSEKKD FYKDGKRLKN YNMPWGAGHN QCLGKSYAIN
460 470 480 490 500
SIKQFVVLLL THFDLELGSE DTEVPEFDLS RYGFGLMQPE EDVPIRYRAR

L
Length:501
Mass (Da):57,047
Last modified:January 1, 1998 - v1
Checksum:iF018F85D3A1B0EBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001607 mRNA. Translation: BAA21717.1.
AK076585 mRNA. Translation: BAC36403.1.
BC062151 mRNA. Translation: AAH62151.1.
CCDSiCCDS17097.1.
RefSeqiNP_032994.1. NM_008968.3.
UniGeneiMm.2339.

Genome annotation databases

EnsembliENSMUST00000018113; ENSMUSP00000018113; ENSMUSG00000017969.
GeneIDi19223.
KEGGimmu:19223.
UCSCiuc008nzl.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiPTGIS_MOUSE
AccessioniPrimary (citable) accession number: O35074
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: October 25, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families