Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O35074

- PTGIS_MOUSE

UniProt

O35074 - PTGIS_MOUSE

Protein

Prostacyclin synthase

Gene

Ptgis

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).

    Catalytic activityi

    (5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: RefGenome
    2. iron ion binding Source: InterPro
    3. monooxygenase activity Source: InterPro
    4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro
    5. prostaglandin-I synthase activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic signaling pathway Source: Ensembl
    2. cellular response to hypoxia Source: UniProtKB
    3. cellular response to interleukin-1 Source: UniProtKB
    4. cellular response to interleukin-6 Source: UniProtKB
    5. negative regulation of inflammatory response Source: UniProtKB
    6. negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
    7. negative regulation of nitric oxide biosynthetic process Source: UniProtKB
    8. positive regulation of angiogenesis Source: UniProtKB
    9. positive regulation of execution phase of apoptosis Source: UniProtKB
    10. positive regulation of peroxisome proliferator activated receptor signaling pathway Source: UniProtKB
    11. prostaglandin biosynthetic process Source: UniProtKB
    12. regulation of apoptotic process Source: RefGenome

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_188623. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prostacyclin synthase (EC:5.3.99.4)
    Alternative name(s):
    Prostaglandin I2 synthase
    Gene namesi
    Name:Ptgis
    Synonyms:Cyp8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1097156. Ptgis.

    Subcellular locationi

    GO - Cellular componenti

    1. caveola Source: UniProtKB
    2. endoplasmic reticulum Source: UniProtKB
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. extracellular space Source: UniProtKB
    5. integral component of membrane Source: UniProtKB-KW
    6. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501Prostacyclin synthasePRO_0000051911Add
    BLAST

    Proteomic databases

    MaxQBiO35074.
    PaxDbiO35074.
    PRIDEiO35074.

    PTM databases

    PhosphoSiteiO35074.

    Expressioni

    Gene expression databases

    ArrayExpressiO35074.
    BgeeiO35074.
    CleanExiMM_PTGIS.
    GenevestigatoriO35074.

    Interactioni

    Protein-protein interaction databases

    IntActiO35074. 1 interaction.
    MINTiMINT-4109067.
    STRINGi10090.ENSMUSP00000085357.

    Structurei

    3D structure databases

    ProteinModelPortaliO35074.
    SMRiO35074. Positions 24-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1 – 2121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00550000074551.
    HOGENOMiHOG000231026.
    HOVERGENiHBG051100.
    KOiK01831.
    TreeFamiTF105090.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR024204. Cyt_P450_CYP7A1-type.
    IPR002403. Cyt_P450_E_grp-IV.
    IPR027286. PTGIS.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
    PIRSF500628. PTGIS. 1 hit.
    PRINTSiPR00465. EP450IV.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O35074-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSWAALLGLL AVLLLLLLLL SRRRARRPGE PPLDLGSIPW LGHALEFGRD    50
    AASFLTRMKE KHGDIFTVLV GGRYVTVLLD PHSYDTVVWE LRTRLDFHPY 100
    AIFLMERIFD LQLPNFNPSE EKARMKPTLM HRDLQALTEA MYTNLRTVLL 150
    GDSTEAGSGW QETGLLEFSY NALLSAGYLT LYGVEASPRT HESQAQDRVH 200
    SADVFHTFRQ LDLLLPKLAR GSLSAGDKDH ACSVKNRLWK LLSPARLASR 250
    ADRSSWLESY LRHLEEMGVS EEMQARALVL QLWATQGNMG PTAFWLLLFL 300
    LKNPEALAAV RAELKHTVWQ AEQPVSQMTT LPQKILDSMP VLDSVLNETL 350
    RLTAAPFITR EVMADLALPM ADGREFSLRR GDRLLLFPFL SPQKDPEIYT 400
    EPEVFKYNRF LNPDGSEKKD FYKDGKRLKN YNMPWGAGHN QCLGKSYAIN 450
    SIKQFVVLLL THFDLELGSE DTEVPEFDLS RYGFGLMQPE EDVPIRYRAR 500
    L 501
    Length:501
    Mass (Da):57,047
    Last modified:January 1, 1998 - v1
    Checksum:iF018F85D3A1B0EBB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001607 mRNA. Translation: BAA21717.1.
    AK076585 mRNA. Translation: BAC36403.1.
    BC062151 mRNA. Translation: AAH62151.1.
    CCDSiCCDS17097.1.
    RefSeqiNP_032994.1. NM_008968.3.
    UniGeneiMm.2339.

    Genome annotation databases

    EnsembliENSMUST00000018113; ENSMUSP00000018113; ENSMUSG00000017969.
    GeneIDi19223.
    KEGGimmu:19223.
    UCSCiuc008nzl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001607 mRNA. Translation: BAA21717.1 .
    AK076585 mRNA. Translation: BAC36403.1 .
    BC062151 mRNA. Translation: AAH62151.1 .
    CCDSi CCDS17097.1.
    RefSeqi NP_032994.1. NM_008968.3.
    UniGenei Mm.2339.

    3D structure databases

    ProteinModelPortali O35074.
    SMRi O35074. Positions 24-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35074. 1 interaction.
    MINTi MINT-4109067.
    STRINGi 10090.ENSMUSP00000085357.

    PTM databases

    PhosphoSitei O35074.

    Proteomic databases

    MaxQBi O35074.
    PaxDbi O35074.
    PRIDEi O35074.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018113 ; ENSMUSP00000018113 ; ENSMUSG00000017969 .
    GeneIDi 19223.
    KEGGi mmu:19223.
    UCSCi uc008nzl.1. mouse.

    Organism-specific databases

    CTDi 5740.
    MGIi MGI:1097156. Ptgis.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00550000074551.
    HOGENOMi HOG000231026.
    HOVERGENi HBG051100.
    KOi K01831.
    TreeFami TF105090.

    Enzyme and pathway databases

    Reactomei REACT_188623. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

    Miscellaneous databases

    NextBioi 296004.
    PROi O35074.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35074.
    Bgeei O35074.
    CleanExi MM_PTGIS.
    Genevestigatori O35074.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR024204. Cyt_P450_CYP7A1-type.
    IPR002403. Cyt_P450_E_grp-IV.
    IPR027286. PTGIS.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
    PIRSF500628. PTGIS. 1 hit.
    PRINTSi PR00465. EP450IV.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Inverse gene expression of prostacyclin and thromboxane synthases in resident and activated peritoneal macrophages."
      Kuwamoto S., Inoue H., Tone Y., Izumi Y., Tanabe T.
      FEBS Lett. 409:242-246(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Peritoneal macrophage.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Limb.

    Entry informationi

    Entry nameiPTGIS_MOUSE
    AccessioniPrimary (citable) accession number: O35074
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3