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Protein

Claudin-4

Gene

Cldn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-4
Alternative name(s):
Clostridium perfringens enterotoxin receptor
Short name:
CPE-R
Short name:
CPE-receptor
Gene namesi
Name:Cldn4
Synonyms:Cper, Cpetr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1313314. Cldn4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821HelicalSequence analysisAdd
BLAST
Topological domaini29 – 8153ExtracellularSequence analysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Topological domaini103 – 11614CytoplasmicSequence analysisAdd
BLAST
Transmembranei117 – 13721HelicalSequence analysisAdd
BLAST
Topological domaini138 – 16023ExtracellularSequence analysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence analysisAdd
BLAST
Topological domaini182 – 21029CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • apicolateral plasma membrane Source: UniProtKB
  • basal plasma membrane Source: Ensembl
  • bicellular tight junction Source: UniProtKB
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: MGI
  • lateral plasma membrane Source: Ensembl
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Claudin-4PRO_0000144744Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei209 – 2091Phosphotyrosine; by EPHA2By similarity

Post-translational modificationi

Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35054.
PRIDEiO35054.

PTM databases

iPTMnetiO35054.
PhosphoSiteiO35054.

Expressioni

Tissue specificityi

Expressed primarily in lung and kidney.1 Publication

Gene expression databases

BgeeiO35054.
CleanExiMM_CLDN4.
ExpressionAtlasiO35054. baseline and differential.
GenevisibleiO35054. MM.

Interactioni

Subunit structurei

Interacts with EPHA2; phosphorylates CLDN4 and may regulate tight junctions (By similarity). Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-40781N.
MINTiMINT-113301.
STRINGi10090.ENSMUSP00000053420.

Structurei

3D structure databases

ProteinModelPortaliO35054.
SMRiO35054. Positions 5-184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 103103Interaction with EPHA2By similarityAdd
BLAST
Regioni209 – 2102Interactions with TJP1, TJP2 and TJP3By similarity

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIJR. Eukaryota.
ENOG410YA61. LUCA.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiO35054.
KOiK06087.
OMAiTAHNVIR.
OrthoDBiEOG79KPGQ.
PhylomeDBiO35054.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003550. Claudin4.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01379. CLAUDIN4.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35054-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASMGLQVLG ISLAVLGWLG IILSCALPMW RVTAFIGSNI VTAQTSWEGL
60 70 80 90 100
WMNCVVQSTG QMQCKMYDSM LALPQDLQAA RALMVISIIV GALGMLLSVV
110 120 130 140 150
GGKCTNCMED ETVKAKIMIT AGAVFIVASM LIMVPVSWTA HNVIRDFYNP
160 170 180 190 200
MVASGQKREM GASLYVGWAA SGLLLLGGGL LCCSCPPRSN DKPYSAKYSA
210
ARSVPASNYV
Length:210
Mass (Da):22,339
Last modified:January 1, 1998 - v1
Checksum:i3B6D571EC71D6564
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000713 mRNA. Translation: BAA22985.1.
AF087822 mRNA. Translation: AAD09757.1.
CCDSiCCDS19728.1.
RefSeqiNP_034033.1. NM_009903.2.
UniGeneiMm.7339.

Genome annotation databases

EnsembliENSMUST00000051401; ENSMUSP00000053420; ENSMUSG00000047501.
GeneIDi12740.
KEGGimmu:12740.
UCSCiuc008zxd.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000713 mRNA. Translation: BAA22985.1.
AF087822 mRNA. Translation: AAD09757.1.
CCDSiCCDS19728.1.
RefSeqiNP_034033.1. NM_009903.2.
UniGeneiMm.7339.

3D structure databases

ProteinModelPortaliO35054.
SMRiO35054. Positions 5-184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-40781N.
MINTiMINT-113301.
STRINGi10090.ENSMUSP00000053420.

PTM databases

iPTMnetiO35054.
PhosphoSiteiO35054.

Proteomic databases

PaxDbiO35054.
PRIDEiO35054.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051401; ENSMUSP00000053420; ENSMUSG00000047501.
GeneIDi12740.
KEGGimmu:12740.
UCSCiuc008zxd.3. mouse.

Organism-specific databases

CTDi1364.
MGIiMGI:1313314. Cldn4.

Phylogenomic databases

eggNOGiENOG410IIJR. Eukaryota.
ENOG410YA61. LUCA.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiO35054.
KOiK06087.
OMAiTAHNVIR.
OrthoDBiEOG79KPGQ.
PhylomeDBiO35054.
TreeFamiTF331936.

Miscellaneous databases

ChiTaRSiCldn4. mouse.
PROiO35054.
SOURCEiSearch...

Gene expression databases

BgeeiO35054.
CleanExiMM_CLDN4.
ExpressionAtlasiO35054. baseline and differential.
GenevisibleiO35054. MM.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003550. Claudin4.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01379. CLAUDIN4.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Clostridium perfringens enterotoxin utilizes two structurally related membrane proteins as functional receptors in vivo."
    Katahira J., Sugiyama H., Inoue N., Horiguchi Y., Matsuda M., Sugimoto N.
    J. Biol. Chem. 272:26652-26658(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands."
    Morita K., Furuse M., Fujimoto K., Tsukita S.
    Proc. Natl. Acad. Sci. U.S.A. 96:511-516(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Kidney.
  3. "Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins."
    Itoh M., Furuse M., Morita K., Kubota K., Saitou M., Tsukita S.
    J. Cell Biol. 147:1351-1363(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TJP1; TJP2 AND TJP3.
  4. "EphA2 phosphorylates the cytoplasmic tail of Claudin-4 and mediates paracellular permeability."
    Tanaka M., Kamata R., Sakai R.
    J. Biol. Chem. 280:42375-42382(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TJP1.

Entry informationi

Entry nameiCLD4_MOUSE
AccessioniPrimary (citable) accession number: O35054
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.