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Protein

Claudin-4

Gene

Cldn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney (PubMed:20921420). Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts (PubMed:20921420). Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.1 Publication

GO - Molecular functioni

  • chloride channel activity Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • structural molecule activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel
Biological processIon transport, Transport
LigandChloride

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-41 Publication
Alternative name(s):
Clostridium perfringens enterotoxin receptor1 Publication
Short name:
CPE-R1 Publication
Short name:
CPE-receptor1 Publication
Gene namesi
Name:Cldn4Imported
Synonyms:Cper1 Publication, Cpetr11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1313314 Cldn4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Topological domaini29 – 81ExtracellularSequence analysisAdd BLAST53
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 116CytoplasmicSequence analysisAdd BLAST14
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 160ExtracellularSequence analysisAdd BLAST23
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Topological domaini182 – 210CytoplasmicSequence analysisAdd BLAST29

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31R → T: No effect. 1 Publication1
Mutagenesisi48E → Q: No effect. 1 Publication1
Mutagenesisi65K → T: Abolishes ability to form paracellular chloride channel. 1 Publication1
Mutagenesisi68D → N: No effect. 1 Publication1
Mutagenesisi76D → N: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447441 – 210Claudin-4Add BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199PhosphoserineBy similarity1
Modified residuei209Phosphotyrosine; by EPHA2By similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35054
PRIDEiO35054

PTM databases

iPTMnetiO35054
PhosphoSitePlusiO35054

Expressioni

Tissue specificityi

Expressed primarily in lung and kidney (PubMed:9892664). Present in both cortical and medullar collecting ducts (at protein level) (PubMed:20921420).2 Publications

Gene expression databases

BgeeiENSMUSG00000047501
CleanExiMM_CLDN4
ExpressionAtlasiO35054 baseline and differential
GenevisibleiO35054 MM

Interactioni

Subunit structurei

Interacts with EPHA2; phosphorylates CLDN4 and may regulate tight junctions (By similarity). Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (PubMed:10601346, PubMed:16236711). Interacts with CLDN1 (By similarity). Interacts with CLDN4 (PubMed:20921420).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-40781N
IntActiO35054, 1 interactor
MINTiO35054
STRINGi10090.ENSMUSP00000053420

Structurei

3D structure databases

ProteinModelPortaliO35054
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 103Interaction with EPHA2By similarityAdd BLAST103
Regioni209 – 210Interactions with TJP1, TJP2 and TJP3By similarity2

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIJR Eukaryota
ENOG410YA61 LUCA
GeneTreeiENSGT00760000118928
HOGENOMiHOG000220937
HOVERGENiHBG000643
InParanoidiO35054
KOiK06087
OMAiTAHNVIR
OrthoDBiEOG091G0MMR
PhylomeDBiO35054
TreeFamiTF331936

Family and domain databases

InterProiView protein in InterPro
IPR006187 Claudin
IPR003550 Claudin4
IPR017974 Claudin_CS
IPR004031 PMP22/EMP/MP20/Claudin
PANTHERiPTHR12002 PTHR12002, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01379 CLAUDIN4
PROSITEiView protein in PROSITE
PS01346 CLAUDIN, 1 hit

Sequencei

Sequence statusi: Complete.

O35054-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASMGLQVLG ISLAVLGWLG IILSCALPMW RVTAFIGSNI VTAQTSWEGL
60 70 80 90 100
WMNCVVQSTG QMQCKMYDSM LALPQDLQAA RALMVISIIV GALGMLLSVV
110 120 130 140 150
GGKCTNCMED ETVKAKIMIT AGAVFIVASM LIMVPVSWTA HNVIRDFYNP
160 170 180 190 200
MVASGQKREM GASLYVGWAA SGLLLLGGGL LCCSCPPRSN DKPYSAKYSA
210
ARSVPASNYV
Length:210
Mass (Da):22,339
Last modified:January 1, 1998 - v1
Checksum:i3B6D571EC71D6564
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000713 mRNA Translation: BAA22985.1
AF087822 mRNA Translation: AAD09757.1
CCDSiCCDS19728.1
RefSeqiNP_034033.1, NM_009903.2
UniGeneiMm.7339

Genome annotation databases

EnsembliENSMUST00000051401; ENSMUSP00000053420; ENSMUSG00000047501
GeneIDi12740
KEGGimmu:12740
UCSCiuc008zxd.3 mouse

Similar proteinsi

Entry informationi

Entry nameiCLD4_MOUSE
AccessioniPrimary (citable) accession number: O35054
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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