Reviewed,
UniProtKB/Swiss-Prot O35052 (CDS1_RAT)
Last modified
November 3, 2009.
Version 67.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Phosphatidate cytidylyltransferase 1 EC=2.7.7.41 Alternative name(s): CDP-diglyceride pyrophosphorylase 1 CDP-diglyceride synthetase 1 CDP-diacylglycerol synthase 1 Short name=CDS 1 CTP:phosphatidate cytidylyltransferase 1 CDP-DG synthetase 1 CDP-DAG synthase 1 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 461 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Provides CDP-diacylglycerol an important precursor for the synthesis of phosphatidylinositol (PtdIns), phosphatidylglycerol, and cardiolipin. Overexpression may amplify cellular signaling responses from cytokines. May also play an important role in the signal transduction mechanism of retina and neural cells By similarity. May play an important role in phosphoinositide synthesis, prefers 1-stearoyl-2-arachidonoyl phosphatidic acid as substrate. |
| Catalytic activity | CTP + phosphatidate = diphosphate + CDP-diacylglycerol. |
| Cofactor | Magnesium. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Cytoplasmic aspect of the endoplasmic reticulum. Ref.4 |
| Tissue specificity | Brain, retina and testis. Found in cerebellar Purkinje cells, pineal body, inner segment of photoreceptor cells and postmitotic spermatocytes and spermatids. |
| Induction | Activated by GTP. Inhibited by CDP-diacylglycerol (product inhibition). |
| Sequence similarities | Belongs to the CDS family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Phospholipid biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | Magnesium |
| Molecular function | Nucleotidyltransferase Transferase |
| Gene Ontology (GO) | |
| Biological process | phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Ref.1 Inferred from direct assay. Source: RGD integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidate cytidylyltransferase activity Ref.5Inferred from direct assay. Source: RGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 461 | 461 | Phosphatidate cytidylyltransferase 1 | PRO_0000090715 | |||||
Regions | |||||||||
| Transmembrane | 96 – 116 | 21 | Potential | ||||||
| Transmembrane | 149 – 169 | 21 | Potential | ||||||
| Transmembrane | 183 – 203 | 21 | Potential | ||||||
| Transmembrane | 230 – 250 | 21 | Potential | ||||||
| Transmembrane | 279 – 299 | 21 | Potential | ||||||
| Transmembrane | 357 – 377 | 21 | Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 172 | 1 | R → K in BAA28787. Ref.2 | ||||||
| Sequence conflict | 299 – 308 | 10 | SKYQYFVCPV → VQVSVLCGARW Ref.1 | ||||||
| Sequence conflict | 326 | 1 | L → V in BAA28787. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain." Saito S., Goto K., Tonosaki A., Kondo H. J. Biol. Chem. 272:9503-9509(1997) [PubMed: 9083091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain. |
| [2] | "Rat brain CDP-diacylglycerol synthase." Hosaka K., Imai H., Nikawa J. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver. |
| [4] | "Topography of glycerolipid synthetic enzymes. Synthesis of phosphatidylserine, phosphatidylinositol and glycerolipid intermediates occurs on the cytoplasmic surface of rat liver microsomal vesicles." Ballas L.M., Bell R.M. Biochim. Biophys. Acta 665:586-595(1981) [PubMed: 6271231] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| [5] | "Extraction and stabilization of mammalian CDP-diacylglycerol synthase activity." Monaco M.E., Feldman M. Biochem. Biophys. Res. Commun. 239:166-170(1997) [PubMed: 9345289] [Abstract] Cited for: CHARACTERIZATION. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AB000517 mRNA. Translation: BAA22085.1. AB009999 mRNA. Translation: BAA28787.1. BC127492 mRNA. Translation: AAI27493.1. | |
| IPI | IPI00326213. |
| RefSeq | NP_112521.2. |
| UniGene | Rn.18983 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O35052. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000002918; ENSRNOP00000002918; ENSRNOG00000002142; Rattus norvegicus. [Genome view] |
| GeneID | 81925. |
| KEGG | rno:81925. |
| UCSC | NM_031242. rat. |
Organism-specific databases | |
| CTD | 81925. |
| RGD | 621185. Cds1. |
Phylogenomic databases | |
| HOVERGEN | O35052. |
| OMA | IEISPKK. |
Enzyme and pathway databases | |
| BRENDA | 2.7.7.41. 248. |
Gene expression databases | |
| ArrayExpress | O35052. |
| Genevestigator | O35052. |
| GermOnline | ENSRNOG00000002142. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000374. PC_trans. IPR016720. PC_Trfase_euk. [Graphical view] |
| Pfam | PF01148. CTP_transf_1. 1 hit. [Graphical view] |
| PIRSF | PIRSF018269. PC_trans_euk. 1 hit. |
| PROSITE | PS01315. CDS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 615829. |
Entry information
| Entry name | CDS1_RAT | ||||||||
| Accession | Primary (citable) accession number: O35052 Secondary accession number(s): A0JPL6, O88208 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


