Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable coenzyme A biosynthesis bifunctional protein CoaBC

Gene

coaBC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine (By similarity).By similarity

Catalytic activityi

N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2.
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase (coaA), Type III pantothenate kinase (coaX)
  2. Probable coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  3. Probable coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  4. Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei124 – 1241SubstrateBy similarity
Active sitei157 – 1571Proton donorBy similarity
Binding sitei281 – 2811CTPBy similarity
Binding sitei291 – 2911CTPBy similarity
Binding sitei339 – 3391CTPBy similarity
Binding sitei343 – 3431CTPBy similarity

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Decarboxylase, Ligase, Lyase

Keywords - Ligandi

Flavoprotein, FMN, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15700-MONOMER.
UniPathwayiUPA00241; UER00353.
UPA00241; UER00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable coenzyme A biosynthesis bifunctional protein CoaBC
Alternative name(s):
DNA/pantothenate metabolism flavoprotein
Including the following 2 domains:
Phosphopantothenoylcysteine decarboxylase (EC:4.1.1.36)
Short name:
PPCDC
Alternative name(s):
CoaC
Phosphopantothenate--cysteine ligase (EC:6.3.2.5)
Alternative name(s):
CoaB
PPC synthetase
Short name:
PPCS
Phosphopantothenoylcysteine synthase
Gene namesi
Name:coaBC
Synonyms:yloI
Ordered Locus Names:BSU15700
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406Probable coenzyme A biosynthesis bifunctional protein CoaBCPRO_0000387986Add
BLAST

Proteomic databases

PaxDbiO35033.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008671.

Structurei

3D structure databases

ProteinModelPortaliO35033.
SMRiO35033. Positions 1-181, 184-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 188188Phosphopantothenoylcysteine decarboxylaseBy similarityAdd
BLAST
Regioni189 – 406218Phosphopantothenate--cysteine ligaseBy similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.Curated
In the C-terminal section; belongs to the PPC synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJS. Bacteria.
COG0452. LUCA.
HOGENOMiHOG000037525.
InParanoidiO35033.
KOiK13038.
OMAiVRFIGNH.
OrthoDBiEOG6FFSBB.
PhylomeDBiO35033.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
3.40.50.1950. 1 hit.
InterProiIPR005252. CoaBC.
IPR007085. DNA/pantothenate-metab_flavo_C.
IPR003382. Flavoprotein.
[Graphical view]
PfamiPF04127. DFP. 1 hit.
PF02441. Flavoprotein. 1 hit.
[Graphical view]
SUPFAMiSSF102645. SSF102645. 1 hit.
SSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00521. coaBC_dfp. 1 hit.

Sequencei

Sequence statusi: Complete.

O35033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNNRNVLLC VSGGIAVYKA CALTSKLVQA GANVKVIMTE SACRFVSPLT
60 70 80 90 100
FQALSRHEVY TDTFKEQNPS VISHIDAADW ADLIIVAPAT ANVIGKLANG
110 120 130 140 150
IADDMLTTTL LAATAPVWIA PAMNVHMYDH PAVKRNISVL YQDGYCFIEP
160 170 180 190 200
SEGYLACGYV GKGRLEEPEN IVKLAEKHFA EETSAPLEGK HVVITAGPTR
210 220 230 240 250
EAIDPVRFFT NKSTGKMGYA LAEAAVQLGA RVILISGPVS LDQPKGLAEF
260 270 280 290 300
IPVQSAADMR EAVLSVYDAS DIVIKTAAVA DFTPKTVFDH KMKKQDGGMT
310 320 330 340 350
LELKRTVDIL KELGEKKKEQ ILVGFAAETQ DIEHYARKKL AAKNLDLIVA
360 370 380 390 400
NDVKANGAGF GADTNIVTIF FKDGHKRELP IMSKLDVSFE ILQEIAALSK

QTGERS
Length:406
Mass (Da):43,978
Last modified:January 1, 1998 - v1
Checksum:i10EC58AD6EEE3FF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13937 Genomic DNA. Translation: CAA74260.1.
AL009126 Genomic DNA. Translation: CAB13443.1.
PIRiD69878.
RefSeqiNP_389452.1. NC_000964.3.
WP_009967219.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13443; CAB13443; BSU15700.
GeneIDi938487.
KEGGibsu:BSU15700.
PATRICi18974947. VBIBacSub10457_1665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13937 Genomic DNA. Translation: CAA74260.1.
AL009126 Genomic DNA. Translation: CAB13443.1.
PIRiD69878.
RefSeqiNP_389452.1. NC_000964.3.
WP_009967219.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO35033.
SMRiO35033. Positions 1-181, 184-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008671.

Proteomic databases

PaxDbiO35033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13443; CAB13443; BSU15700.
GeneIDi938487.
KEGGibsu:BSU15700.
PATRICi18974947. VBIBacSub10457_1665.

Phylogenomic databases

eggNOGiENOG4105CJS. Bacteria.
COG0452. LUCA.
HOGENOMiHOG000037525.
InParanoidiO35033.
KOiK13038.
OMAiVRFIGNH.
OrthoDBiEOG6FFSBB.
PhylomeDBiO35033.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00353.
UPA00241; UER00354.
BioCyciBSUB:BSU15700-MONOMER.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
3.40.50.1950. 1 hit.
InterProiIPR005252. CoaBC.
IPR007085. DNA/pantothenate-metab_flavo_C.
IPR003382. Flavoprotein.
[Graphical view]
PfamiPF04127. DFP. 1 hit.
PF02441. Flavoprotein. 1 hit.
[Graphical view]
SUPFAMiSSF102645. SSF102645. 1 hit.
SSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00521. coaBC_dfp. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A 28 kbp segment from the spoVM region of the Bacillus subtilis 168 genome."
    Foulger D., Errington J.
    Microbiology 144:801-805(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiCOABC_BACSU
AccessioniPrimary (citable) accession number: O35033
Secondary accession number(s): Q799L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: January 1, 1998
Last modified: February 17, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.