Reviewed, UniProtKB/Swiss-Prot O35031 (ACYP_BACSU)

Names and origin

Protein names

Recommended name:
    Acylphosphatase
    EC=3.6.1.7
Alternative name(s):
    Acylphosphate phosphohydrolase

Gene names

Name:	acyP
Synonyms:	yflL
Ordered Locus Names:	BSU07640

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	91 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An acylphosphate + H2O = a carboxylate + phosphate. HAMAP MF_01450
Sequence similarities	Belongs to the acylphosphatase family. Contains 1 acylphosphatase-like domain.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Molecular function	acylphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 91

91

Acylphosphatase HAMAP MF_01450

PRO_0000158552

Regions

Domain

3 – 90

88

Acylphosphatase-like

Sites

Active site

18

1

By similarity

Active site

36

1

By similarity

Secondary structure

1

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91

Helix Strand Turn

Details...

Beta strand

2 – 11

10

Beta strand

13 – 16

4

Helix

17 – 27

11

Beta strand

31 – 36

6

Beta strand

42 – 48

7

Helix

50 – 62

13

Beta strand

68 – 78

11

Beta strand

85 – 88

4

Sequences

	Sequence		Length	Mass (Da)
	O35031-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 50795631BF3310F4	FASTA	91	10,318
	10 20 30 40 50 60 MLQYRIIVDG RVQGVGFRYF VQMEADKRKL AGWVKNRDDG RVEILAEGPE NALQSFVEAV 70 80 90 KNGSPFSKVT DISVTESRSL EGHHRFSIVY S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination proteins, an iron uptake system and a general stress response protein." Yamamoto H., Uchiyama S., Nugroho F.A., Sekiguchi J. Gene 194:191-199(1997) [PubMed: 9272861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / AC327.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D86417 Genomic DNA. Translation: BAA22305.1.
AL009126 Genomic DNA. Translation: CAB12593.1.

PIR

B69811.

RefSeq

NP_388645.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FHM	NMR	-	A	1-91	[»]
2HLT	NMR	-	A	1-91	[»]
2HLU	NMR	-	A	1-91	[»]
3BR8	X-ray	1.33	A	1-91	[»]

ModBase

Search...

Genome annotation databases

GeneID

939684.

GenomeReviews

Gene locus BSU07640 in contig AL009126_GR.

KEGG

bsu:BSU07640.

NMPDR

fig|224308.1.peg.764.

Organism-specific databases

SubtiList

BG12947. yflL. [Micado]

CMR

Search...

Phylogenomic databases

HOGENOM

O35031.

OMA

PFSKVTD.

Enzyme and pathway databases

BioCyc

BSUB224308:BSU0764-MON.

BRENDA

3.6.1.7. 150.

Family and domain databases

HAMAP

MF_01450.
[Tree]

InterPro

IPR020456. Acylphosphatase.
IPR001792. Acylphosphatase-like.
IPR017968. Acylphosphatase_CS.
[Graphical view]

PANTHER

PTHR10029. Acylphosphatase. 1 hit.

Pfam

PF00708. Acylphosphatase. 1 hit.
[Graphical view]

PRINTS

PR00112. ACYLPHPHTASE.

ProDom

PD001884. Acylphosphatase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00150. ACYLPHOSPHATASE_1. 1 hit.
PS00151. ACYLPHOSPHATASE_2. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ACYP_BACSU

Accession

Primary (citable) accession number: O35031

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)